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  1. Article ; Online: The status surrounding chloroquine and other drugs as potential anti-infective agents for COVID-19.

    Ramotar, Dindial

    Expert review of respiratory medicine

    2020  Volume 14, Issue 9, Page(s) 863–864

    Keywords covid19
    Language English
    Publishing date 2020-06-30
    Publishing country England
    Document type Editorial
    ZDB-ID 2479146-5
    ISSN 1747-6356 ; 1747-6348
    ISSN (online) 1747-6356
    ISSN 1747-6348
    DOI 10.1080/17476348.2020.1782199
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  3. Article ; Online: The status surrounding chloroquine and other drugs as potential anti-infective agents for COVID-19

    Ramotar, Dindial

    Expert Review of Respiratory Medicine

    2020  Volume 14, Issue 9, Page(s) 863–864

    Keywords Immunology and Allergy ; Public Health, Environmental and Occupational Health ; Pulmonary and Respiratory Medicine ; covid19
    Language English
    Publisher Informa UK Limited
    Publishing country uk
    Document type Article ; Online
    ZDB-ID 2479146-5
    ISSN 1747-6356 ; 1747-6348
    ISSN (online) 1747-6356
    ISSN 1747-6348
    DOI 10.1080/17476348.2020.1782199
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  4. Article ; Online: The histone H2B Arg95 residue efficiently recruits the transcription factor Spt16 to mediate Ste5 expression of the pheromone response pathway.

    Sulaiman, Abdallah Alhaj / Ali, Reem / Ramotar, Dindial

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 10189

    Abstract: In yeast Saccharomyces cerevisiae, the immunosuppressant rapamycin inhibits the TORC1 kinase causing rapid alteration in gene expression and leading to ... ...

    Abstract In yeast Saccharomyces cerevisiae, the immunosuppressant rapamycin inhibits the TORC1 kinase causing rapid alteration in gene expression and leading to G
    MeSH term(s) Histones/genetics ; Histones/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Nucleosomes/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Transcriptional Elongation Factors/metabolism ; Chromatin/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Pheromones/pharmacology ; Pheromones/metabolism ; Sirolimus/pharmacology ; Sirolimus/metabolism ; Adaptor Proteins, Signal Transducing/metabolism
    Chemical Substances Histones ; Transcription Factors ; Nucleosomes ; Saccharomyces cerevisiae Proteins ; Transcriptional Elongation Factors ; Chromatin ; Pheromones ; Sirolimus (W36ZG6FT64) ; STE5 protein, S cerevisiae ; Adaptor Proteins, Signal Transducing
    Language English
    Publishing date 2023-06-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-37339-y
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  5. Article ; Online: A Screening Method to Identify Essential Yeast Genes for Responses Towards Spermine.

    Aouida, Mustapha / Ramotar, Dindial

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2377, Page(s) 363–369

    Abstract: We exploited the yeast DAmP mutant collection to identify essential genes that play a role in polyamine resistance. Herein, we described in details the methodology to obtain these genes. This approach is applicable for screening many nontoxic and toxic ... ...

    Abstract We exploited the yeast DAmP mutant collection to identify essential genes that play a role in polyamine resistance. Herein, we described in details the methodology to obtain these genes. This approach is applicable for screening many nontoxic and toxic drugs.
    MeSH term(s) Genes, Essential ; Polyamines ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Spermine
    Chemical Substances Polyamines ; Saccharomyces cerevisiae Proteins ; Spermine (2FZ7Y3VOQX)
    Language English
    Publishing date 2021-10-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1720-5_20
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  6. Article ; Online: The histone H2B Arg95 residue efficiently recruits the transcription factor Spt16 to mediate Ste5 expression of the pheromone response pathway

    Abdallah Alhaj Sulaiman / Reem Ali / Dindial Ramotar

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 13

    Abstract: Abstract In yeast Saccharomyces cerevisiae, the immunosuppressant rapamycin inhibits the TORC1 kinase causing rapid alteration in gene expression and leading to G1 arrest. We recently reported the isolation and characterization from the histone mutant ... ...

    Abstract Abstract In yeast Saccharomyces cerevisiae, the immunosuppressant rapamycin inhibits the TORC1 kinase causing rapid alteration in gene expression and leading to G1 arrest. We recently reported the isolation and characterization from the histone mutant collection of a histone H2B R95A mutant that displays resistance to rapamycin. This mutant is defective in the expression of several genes belonging to the pheromone response pathway including STE5 encoding a scaffold protein that promotes the activation of downstream MAP kinases. Cells lacking Ste5 cannot arrest the cell cycle in response to rapamycin and as a consequence exhibit similar resistance to rapamycin as the H2B R95A mutant. Herein, we show that the H2B R95A mutation weakens the association of H2B with Spt16 a component of the FACT complex (FAcilitates Chromatin Transcription), and an essential factor that interacts with the histone H2A-H2B dimer to promote transcription and preserve chromatin integrity. From a collection of spt16 mutants, spt16 E857K and spt16-11 showed striking sensitivity to rapamycin as compared to the parent strain. spt16 E857K and spt16-11 expressed distinct forms of Ste5, while a suppressor mutation H2B A84D of the spt16-11 mutant prevents the expression of Ste5 and confers marked resistance to rapamycin. We interpret these findings to suggest that the Arg95 residue of histone H2B is required to recruit Spt16 to maintain the expression of STE5, which performs a role to arrest cells in the G1 phase in response to rapamycin.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
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  7. Article: Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity.

    Sulaiman, Abdallah Alhaj / Al-Ansari, Dana E / Ali, Reem / Aouida, Mustapha / Ramotar, Dindial

    Frontiers in genetics

    2024  Volume 14, Page(s) 1296383

    Abstract: Quinoxaline is a heterocyclic compound with a two-membered ring structure that undergoes redox cycling to produce toxic free radicals. It has antiviral, antibacterial, antifungal, and antitumor activities. However, the biological functions that are ... ...

    Abstract Quinoxaline is a heterocyclic compound with a two-membered ring structure that undergoes redox cycling to produce toxic free radicals. It has antiviral, antibacterial, antifungal, and antitumor activities. However, the biological functions that are involved in mounting a response against the toxic effects of quinoxaline have not been investigated. Herein, we performed a genome-wide screen using the yeast haploid mutant collection and reported the identification of 12 mutants that displayed varying sensitivity towards quinoxaline. No mutant was recovered that showed resistance to quinoxaline. The quinoxaline-sensitive mutants were deleted for genes that encode cell cycle function, as well as genes that belong to other physiological pathways such as the vacuolar detoxification process. Three of the highly sensitive gene-deletion mutants lack the
    Language English
    Publishing date 2024-01-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2023.1296383
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  8. Article ; Online: Caenorhabditis elegans organic cation transporter-2 is a novel drug uptake transporter that mediates induced mutagenesis by environmental genotoxic compounds

    Dindial Ramotar

    Journal of Radiation and Cancer Research, Vol 8, Iss 1, Pp 61-

    2017  Volume 73

    Abstract: Uptake transporters are being studied for roles in the entry of therapeutic drugs into cells and thus can be exploited to improve the treatment of various diseases. The live whole model organism, Caenorhabditis elegans, offers an array of advantages to ... ...

    Abstract Uptake transporters are being studied for roles in the entry of therapeutic drugs into cells and thus can be exploited to improve the treatment of various diseases. The live whole model organism, Caenorhabditis elegans, offers an array of advantages to investigate the roles of these transporters. This organism possesses two organic cation transporters (OCTs), OCT1 and OCT2 that are involved in the uptake of clinically relevant genotoxic anticancer drugs such as doxorubicin and cisplatin into the animal. C. elegans lacking OCT1 displays a shortened lifespan, a decreased brood size, an increased susceptibility to oxidative stress, and certain DNA damaging agents. Remarkably, these phenotypes can be rescued by downregulating the OCT1 paralog, OCT2, leading to the suggestion that OCT1 exerts control on OCT2. Indeed, the loss of OCT1 led to the upregulation of OCT2. OCT2 is an uptake transporter involved in the influx of doxorubicin, as well as a number of other therapeutic agents and chemical compounds, some of which have been identified through ligand-protein docking analyses. The genotoxic compounds entering into C. elegans lead to DNA damage-induced apoptosis of germ cells, a process that can be attenuated by blocking OCT2 function. Thus, by combining the roles of the OCT1 and OCT2 transporters with defects in various DNA repair mechanisms, it is possible to engineer a set of supersensitive C. elegans strains that can serve as the most powerful living sensors to date. These tester C. elegans strains can be used to report on the cytotoxicities and genotoxicities of a battery of old and new drugs developed by pharmaceuticals, undocumented toxicants generated by various industries, and compounds that can cause cancers and are present in trace amounts in the environment around the world. These efforts are attainable as C. elegans can live in the soil and water, and a multitude of tools are available to monitor several readouts from the animals.
    Keywords Caenorhabditis elegans organic cation transporters ; DNA damage and repair pathways ; drug uptake and resistance ; germ cells apoptosis ; Medical physics. Medical radiology. Nuclear medicine ; R895-920 ; Neoplasms. Tumors. Oncology. Including cancer and carcinogens ; RC254-282
    Subject code 571 ; 500
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
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  9. Article ; Online: A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system.

    Aouida, Mustapha / Aljogol, Dina / Ali, Reem / Ramotar, Dindial

    STAR protocols

    2022  Volume 3, Issue 1, Page(s) 101216

    Abstract: Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; (3) ... ...

    Abstract Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; (3) single-cell isolation; and (4) knockout verification to determine indels in one or both alleles by Sanger sequencing. This strategy is based on the efficiency of DNA editing, avoids antibiotic selection, and bypasses the need for cell sorting.
    MeSH term(s) CRISPR-Cas Systems/genetics ; Gene Editing/methods ; Gene Knockout Techniques ; Humans ; Peroxiredoxins/genetics ; RNA, Guide, CRISPR-Cas Systems/genetics ; Transfection
    Chemical Substances RNA, Guide, CRISPR-Cas Systems ; PRDX1 protein, human (EC 1.11.1.15) ; Peroxiredoxins (EC 1.11.1.15)
    Language English
    Publishing date 2022-03-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2022.101216
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  10. Article ; Online: A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system

    Mustapha Aouida / Dina Aljogol / Reem Ali / Dindial Ramotar

    STAR Protocols, Vol 3, Iss 1, Pp 101216- (2022)

    2022  

    Abstract: Summary: Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; ( ...

    Abstract Summary: Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; (3) single-cell isolation; and (4) knockout verification to determine indels in one or both alleles by Sanger sequencing. This strategy is based on the efficiency of DNA editing, avoids antibiotic selection, and bypasses the need for cell sorting.
    Keywords Cell Biology ; Cell culture ; Cell isolation ; Molecular Biology ; CRISPR ; Biotechnology and bioengineering ; Science (General) ; Q1-390
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
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