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Article: Theory of mind and Verstehen (understanding) methodology.

Kumazaki, Tsutomu

2016 Volume 27, Issue 3, Page(s) 253–267

Abstract: Theory of mind is a prominent, but highly controversial, field in psychology, psychiatry, and philosophy of mind. Simulation theory, theory-theory and other views have been presented in recent decades, none of which are monolithic. In this article, ... ...

Abstract	Theory of mind is a prominent, but highly controversial, field in psychology, psychiatry, and philosophy of mind. Simulation theory, theory-theory and other views have been presented in recent decades, none of which are monolithic. In this article, various views on theory of mind are reviewed, and methodological problems within each view are investigated. The relationship between simulation theory and Verstehen (understanding) methodology in traditional human sciences is an intriguing issue, although the latter is not a direct ancestor of the former. From that perspective, lessons for current clinical psychiatry are drawn.
MeSH term(s)	Empathy ; History, 20th Century ; History, 21st Century ; Humans ; Theory of Mind
Language	English
Publishing date	2016
Publishing country	England
Document type	Historical Article ; Journal Article ; Review
ZDB-ID	1028145-9
ISSN	1740-2360 ; 0957-154X
ISSN (online)	1740-2360
ISSN	0957-154X
DOI	10.1177/0957154X16650791
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Article: The theoretical root of Karl Jaspers' General Psychopathology. Part 1: Reconsidering the influence of phenomenology and hermeneutics.

Kumazaki, Tsutomu

History of psychiatry

2014 Volume 24, Issue 2, Page(s) 212–226

Abstract: The present paper investigates the methodology involved in Jaspers' psychopathology and compares it with Husserl's phenomenology and with Dilthey's cultural science. Allgemeine Psychopathologie and other methodological works by Jaspers, the works of ... ...

Abstract	The present paper investigates the methodology involved in Jaspers' psychopathology and compares it with Husserl's phenomenology and with Dilthey's cultural science. Allgemeine Psychopathologie and other methodological works by Jaspers, the works of Husserl and Dilthey that Jaspers cited, and previous research papers on Jaspers are reviewed. Jaspers had conflicting views on understanding, which were comprised of both empathic understanding and rational, ideal-typical understanding. Such a standpoint on understanding is considerably different from Dilthey's. Additionally, the present paper reconfirms that Jaspers' 'phenomenology' as a form of descriptive psychology for the understanding of empirical psychic states is different from Husserl's phenomenology. Thus, this paper casts doubt on the common opinion that Jaspers was under the profound influence of Husserl or Dilthey.
Language	English
Publishing date	2014-02-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1028145-9
ISSN	1740-2360 ; 0957-154X
ISSN (online)	1740-2360
ISSN	0957-154X
DOI	10.1177/0957154X13476201
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Article: The theoretical root of Karl Jaspers' General Psychopathology. Part 2: The influence of Max Weber.

Kumazaki, Tsutomu

History of psychiatry

2013 Volume 24, Issue 3, Page(s) 259–273

Abstract: The present study explores and compares Jaspers' methodology of psychopathology with Weber's methodology of sociology. In his works, Weber incorporated the arguments of many other researchers into his own methodology. Jaspers respected Weber as a mentor ... ...

Abstract	The present study explores and compares Jaspers' methodology of psychopathology with Weber's methodology of sociology. In his works, Weber incorporated the arguments of many other researchers into his own methodology. Jaspers respected Weber as a mentor and presented arguments that were very similar to Weber's. Both Weber and Jaspers began from empathic understanding, but at the same time aimed for a rational and ideal-typical conceptualization. In addition, their methodologies were similar with respect to their detailed terminology. Such similarities cannot be seen with any other scholars. This suggests that Weber may have played an integral role as a mediator between his contemporary scholars and Jaspers. Thus, Weber may have had the most significant influence on Jaspers.
Language	English
Publishing date	2013-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1028145-9
ISSN	1740-2360 ; 0957-154X
ISSN (online)	1740-2360
ISSN	0957-154X
DOI	10.1177/0957154X13482833
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Article: What is a'mood-congruent' delusion? History and conceptual problems.

Kumazaki, Tsutomu

History of psychiatry

2011 Volume 22, Issue 87 Pt 3, Page(s) 315–331

Abstract: This article investigates the history of the concept of mood-congruent delusions and the problems accompanying this concept. In the late nineteenth century, there were conflicting views regarding the relationship between the contents of an individual's ... ...

Abstract	This article investigates the history of the concept of mood-congruent delusions and the problems accompanying this concept. In the late nineteenth century, there were conflicting views regarding the relationship between the contents of an individual's delusional thought and his/her affective state. The differentiation between delusion-like ideas secondary to affective state and incomprehensible primary delusions was introduced in the early twentieth century; this differentiation is the origin of the present-day distinction between mood-congruent and -incongruent delusions. Although the themes of delusions are clearly described in the operational diagnostic criteria for mood-congruent psychotic symptoms, the concept of mood congruence inevitably involves ambiguity. This article argues that a dilemma between reliability and validity emerges when diagnosing mood-congruent (and -incongruent) psychotic symptoms.
MeSH term(s)	Delusions/history ; Europe ; Hallucinations/history ; History, 19th Century ; History, 20th Century ; History, 21st Century ; Humans ; United States
Language	English
Publishing date	2011-09
Publishing country	England
Document type	Historical Article ; Journal Article
ZDB-ID	1028145-9
ISSN	1740-2360 ; 0957-154X
ISSN (online)	1740-2360
ISSN	0957-154X
DOI	10.1177/0957154X10390438
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Article ; Online: Modeling the sake brewing characteristics of rice from brown rice metabolites.

Kobayashi, Takuji / Kumazaki, Tsutomu / Morikawa, Kana / Komatsu-Hata, Yuko / Okuda, Masaki / Takahashi, Masayuki / Saito, Ryota / Oda, Ken / Yazawa, Hisashi / Iwashita, Kazuhiro

Journal of bioscience and bioengineering

2022 Volume 134, Issue 2, Page(s) 116–124

Abstract: Sake, soy sauce, miso (Japanese bean paste), and beer are made from grains. The characteristics of the grain significantly affect the quality of the final product. Many studies have been performed to evaluate the sake-brewing characteristics of rice. ... ...

Abstract	Sake, soy sauce, miso (Japanese bean paste), and beer are made from grains. The characteristics of the grain significantly affect the quality of the final product. Many studies have been performed to evaluate the sake-brewing characteristics of rice. However, current rice analysis methods are time and labor intensive and require large samples. We developed a novel method for predicting the brewing characteristics of sake rice using <1 g of sample. Brown rice extracts were analyzed by ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry, and mass chromatogram data were used as explanatory variables. The objective variables were the physical and chemical properties of the rice, the enzymatic activity of the rice-koji, the fermentation properties of the sake mash, the standard analytical values of the sake, and the flavor component concentrations in the sake. Prediction models were developed using the orthogonal projections to latent structures method. The prediction performances of the models were verified, and 32 out of the 54 objective variables were used in well-performing models. In conclusion, we developed a method for predicting the rice properties and brewing characteristics from results acquired by analyzing <1 g of brown rice. The method is a powerful tool for breeding new sake rice cultivars for good brewing characteristics in early generations and will improve our understanding of fluctuations in the brewing characteristics of sake rice before each sake brewing season starts.
MeSH term(s)	Alcoholic Beverages/analysis ; Fermentation ; Oryza/chemistry ; Saccharomyces cerevisiae/metabolism
Language	English
Publishing date	2022-05-21
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1465387-4
ISSN	1347-4421 ; 1389-1723
ISSN (online)	1347-4421
ISSN	1389-1723
DOI	10.1016/j.jbiosc.2022.04.007
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Article: Detection of mRNA expression and alternative splicing in a single cell.

Kumazaki, Tsutomu

Methods in molecular biology (Clifton, N.J.)

2002 Volume 193, Page(s) 59–64

MeSH term(s)	Alternative Splicing ; RNA, Messenger/analysis ; Reverse Transcriptase Polymerase Chain Reaction/methods
Chemical Substances	RNA, Messenger
Language	English
Publishing date	2002
Publishing country	United States
Document type	Journal Article
ISSN	1064-3745
ISSN	1064-3745
DOI	10.1385/1-59259-283-X:059
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Article: Modeling the sake brewing characteristics of rice from brown rice metabolites

Kobayashi, Takuji / Kumazaki, Tsutomu / Morikawa, Kana / Komatsu-Hata, Yuko / Okuda, Masaki / Takahashi, Masayuki / Saito, Ryota / Oda, Ken / Yazawa, Hisashi / Iwashita, Kazuhiro

The Society for Biotechnology, Japan Journal of bioscience and bioengineering. 2022 Apr. 16,

2022

Abstract	Sake, soy sauce, miso (Japanese bean paste), and beer are made from grains. The characteristics of the grain significantly affect the quality of the final product. Many studies have been performed to evaluate the sake-brewing characteristics of rice. However, current rice analysis methods are time and labor intensive and require large samples. We developed a novel method for predicting the brewing characteristics of sake rice using <1 g of sample. Brown rice extracts were analyzed by ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry, and mass chromatogram data were used as explanatory variables. The objective variables were the physical and chemical properties of the rice, the enzymatic activity of the rice-koji, the fermentation properties of the sake mash, the standard analytical values of the sake, and the flavor component concentrations in the sake. Prediction models were developed using the orthogonal projections to latent structures method. The prediction performances of the models were verified, and 32 out of the 54 objective variables were used in well-performing models. In conclusion, we developed a method for predicting the rice properties and brewing characteristics from results acquired by analyzing <1 g of brown rice. The method is a powerful tool for breeding new sake rice cultivars for good brewing characteristics in early generations and will improve our understanding of fluctuations in the brewing characteristics of sake rice before each sake brewing season starts.
Keywords	beans ; beers ; brown rice ; cultivars ; enzyme activity ; fermentation ; flavor compounds ; mash ; mass spectrometry ; metabolites ; miso ; prediction ; sake ; soy sauce ; ultra-performance liquid chromatography
Language	English
Dates of publication	2022-0416
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	1465387-4
ISSN	1347-4421 ; 1389-1723
ISSN (online)	1347-4421
ISSN	1389-1723
DOI	10.1016/j.jbiosc.2022.04.007
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Article ; Online: Establishment of ultra long-lived cell lines by transfection of TERT into normal human fibroblast TIG-1 and their characterization.

Kamada, Mizuna / Kumazaki, Tsutomu / Matsuo, Taira / Mitsui, Youji / Takahashi, Tomoko

Cell biology international

2012 Volume 36, Issue 6, Page(s) 519–527

Abstract: To establish useful human normal cell lines, TERT (telomerase reverse transcriptase) cDNA was transfected into normal female lung fibroblast, TIG-1. After long-term-sub-cultivation of 74 individual clones selected for resistance to G418, we obtained 55 ... ...

Abstract	To establish useful human normal cell lines, TERT (telomerase reverse transcriptase) cDNA was transfected into normal female lung fibroblast, TIG-1. After long-term-sub-cultivation of 74 individual clones selected for resistance to G418, we obtained 55 cultures with normal range of life span [75 PDL (population doubling level)], 16 cultures with extended life span (75-140 PDL). In addition, 3 immortal cell strains and unexpectedly, one ultra long-lived cell line (ULT-1) with life span of 166 PDL were established. IMT-1, one of the immortal cell strains was confirmed to maintain long telomere length, high telomerase activity and an extremely low level of p16INK4A. They also showed moderate p53 and p21CIP1 expression, keeping vigorous growth rate even at 450 PDL. High level of fibronectin and collagen 1α expression confirmed IMT-1 as normal fibroblasts, although one X chromosome had been lost. ULT-1, however, kept a near normal karyotypes and had shortening of telomere length, high expression of p16INK4A, moderate levels of senescence associated-β-galactosidase positive cells and decreased growth rate only after 150 PDs (population doublings), and finally reached senescence at 166 PDL with morphology of normal senescent fibroblasts. As resources of standard normal human cell, abundant vials of early and middle passages of ULT-1 have been stocked. The use of the cell line is discussed, focusing on isograft of artificial skin and screening of anti-aging or safe chemical agents.
MeSH term(s)	Abnormal Karyotype ; Animals ; Cell Line ; Cell Shape ; Cell Survival ; Cell Transformation, Neoplastic ; Cyclin-Dependent Kinase Inhibitor p21/metabolism ; Female ; Fibroblasts/metabolism ; Fibroblasts/physiology ; Fibroblasts/transplantation ; Gentamicins/pharmacology ; Humans ; Mice ; Mice, SCID ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Telomerase/biosynthesis ; Telomerase/genetics ; Telomere Homeostasis ; Transfection ; Tumor Suppressor Protein p53/metabolism
Chemical Substances	CDKN1A protein, human ; Cyclin-Dependent Kinase Inhibitor p21 ; Gentamicins ; Recombinant Proteins ; TP53 protein, human ; Tumor Suppressor Protein p53 ; antibiotic G 418 (A08F5XTI6G) ; TERT protein, human (EC 2.7.7.49) ; Telomerase (EC 2.7.7.49)
Language	English
Publishing date	2012-06-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1143453-3
ISSN	1095-8355 ; 1065-6995
ISSN (online)	1095-8355
ISSN	1065-6995
DOI	10.1042/CBI20110460
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Article ; Online: LIN54 harboring a mutation in CHC domain is localized to the cytoplasm and inhibits cell cycle progression.

Matsuo, Taira / Kuramoto, Hiroyo / Kumazaki, Tsutomu / Mitsui, Youji / Takahashi, Tomoko

Cell cycle (Georgetown, Tex.)

2012 Volume 11, Issue 17, Page(s) 3227–3236

Abstract: The mammalian LIN complex (LINC) plays important roles in regulation of cell cycle genes. LIN54 is an essential core subunit of the LINC and has a DNA binding region (CHC domain), which consists of two cysteine-rich (CXC) domains separated by a short ... ...

Abstract	The mammalian LIN complex (LINC) plays important roles in regulation of cell cycle genes. LIN54 is an essential core subunit of the LINC and has a DNA binding region (CHC domain), which consists of two cysteine-rich (CXC) domains separated by a short spacer. We generated various LIN54 mutants, such as CHC deletion mutant, and investigated their subcellular localizations and effects on cell cycle. Wild-type LIN54 was predominantly localized in the nucleus. We identified two nuclear localization signals (NLSs), both of which were required for nuclear localization of LIN54. Interestingly, deletion of one CXC domain resulted in an increased cytoplasmic localization. The cytoplasmic LIN54 mutant accumulated in the nucleus after leptomycin B treatment, suggesting CRM1-mediated nuclear export of LIN54. Point mutations (C525Y and C611Y) in conserved cysteine residues of CXC domain that abolish DNA binding activity also increased cytoplasmic localization. These data suggest that DNA binding activity of LIN54 is required for its nuclear retention. We also found that LIN54 (C525Y) and LIN54 (C611Y) inhibited cell cycle progression and led to abnormal nuclear morphology. Other CXC mutants also induced similar abnormalities in cell cycle progression. LIN54 (C525Y) led to a decreased expression of some G2/M genes, whose expressions are regulated by LINC. This cell cycle inhibition was partially restored by overexpression of wild-type LIN54. These results suggest that abnormal cellular localization of LIN54 may have effects on LINC activity.
MeSH term(s)	Amino Acid Sequence ; Animals ; Blotting, Western ; Cell Cycle/genetics ; Cell Cycle/physiology ; Cell Line, Tumor ; Cytoplasm/metabolism ; DNA Primers/genetics ; Flow Cytometry ; Green Fluorescent Proteins ; Humans ; Mice ; Microscopy, Fluorescence ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Nuclear Localization Signals/genetics ; Point Mutation/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Trans-Activators/genetics ; Trans-Activators/metabolism
Chemical Substances	DNA Primers ; Lin54 protein, human ; Nuclear Localization Signals ; Trans-Activators ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2012-08-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2146183-1
ISSN	1551-4005 ; 1538-4101 ; 1554-8627
ISSN (online)	1551-4005
ISSN	1538-4101 ; 1554-8627
DOI	10.4161/cc.21569
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Article ; Online: Tumorigenic risk of human induced pluripotent stem cell explants cultured on mouse SNL76/7 feeder cells.

Kamada, Mizuna / Mitsui, Youji / Kumazaki, Tsutomu / Kawahara, Yuta / Matsuo, Taira / Takahashi, Tomoko

Biochemical and biophysical research communications

2014 Volume 453, Issue 3, Page(s) 668–673

Abstract: The potential for tumor formation from transplanted human induced pluripotent stem cell (hiPSC) derivatives represents a high risk in their application to regenerative medicine. We examined the genetic origin and characteristics of tumors, that were ... ...

Abstract	The potential for tumor formation from transplanted human induced pluripotent stem cell (hiPSC) derivatives represents a high risk in their application to regenerative medicine. We examined the genetic origin and characteristics of tumors, that were formed when 13 hiPSC lines, established by ourselves, and 201B7 hiPSC from Kyoto University were transplanted into severe combined immune-deficient (SCID) mice. Though teratomas formed in 58% of mice, five angiosarcomas, one malignant solitary fibrous tumor and one undifferentiated pleomorphic sarcoma formed in the remaining mice. Three malignant cell lines were established from the tumors, which were derived from mitomycin C (MMC)-treated SNL76/7 (MMC-SNL) feeder cells, as tumor development from fusion cells between MMC-SNL and hiPSCs was negative by genetic analysis. While parent SNL76/7 cells produced malignant tumors, neither MMC-SNL nor MMC-treated mouse embryo fibroblast (MEF) produced malignant tumors. When MMC-SNL feeder cells were co-cultured with hiPSCs, growing cell lines were generated, that expressed genes similar to the parent SNL76/7 cells. Thus, hiPSCs grown on MMC-SNL feeder cells have a high risk of generating feeder-derived malignant tumors. The possible mechanism(s) of growth restoration and the formation of multiple tumor types are discussed with respect of the interactions between MMC-SNL and hiPSC.
MeSH term(s)	Animals ; Carcinogenesis ; Cell Line ; Feeder Cells ; Gene Expression Profiling ; Humans ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism ; Mice ; Mice, SCID ; Neoplasms, Experimental/pathology ; Stem Cell Transplantation
Language	English
Publishing date	2014-10-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2014.10.009
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