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  1. Book ; Thesis: Transkriptionelle Kontrolle der Differenzierung und Funktion follikulärer T-Helferzellen (TFH-Zellen)

    Stauß, Dennis

    2013  

    Author's details von Dennis Stauß
    Language German
    Size 2 Mikrofiches, Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis @Berlin, Humboldt-Univ., Diss., 2013
    Database Special collection on veterinary medicine and general parasitology

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  2. Book ; Thesis: Transkriptionelle Kontrolle der Differenzierung und Funktion follikulärer T-Helferzellen (TFH-Zellen)

    Stauß, Dennis

    2013  

    Author's details von Dennis Stauß
    Language German
    Size 2 Mikrofiches, Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Humboldt-Univ., Diss.--Berlin, 2013
    Database Former special subject collection: coastal and deep sea fishing

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  3. Article ; Online: The transcriptional coactivator Bob1 promotes the development of follicular T helper cells via Bcl6.

    Stauss, Dennis / Brunner, Cornelia / Berberich-Siebelt, Friederike / Höpken, Uta E / Lipp, Martin / Müller, Gerd

    The EMBO journal

    2016  Volume 35, Issue 8, Page(s) 881–898

    Abstract: Follicular T helper (Tfh) cells are key regulators of the germinal center reaction and long-term humoral immunity. Tfh cell differentiation requires the sustained expression of the transcriptional repressor Bcl6; however, its regulation in CD4(+)T cells ... ...

    Abstract Follicular T helper (Tfh) cells are key regulators of the germinal center reaction and long-term humoral immunity. Tfh cell differentiation requires the sustained expression of the transcriptional repressor Bcl6; however, its regulation in CD4(+)T cells is incompletely understood. Here, we report that the transcriptional coactivator Bob1, encoded by thePou2af1gene, promotes Bcl6 expression and Tfh cell development. We found that Bob1 together with the octamer transcription factors Oct1/Oct2 can directly bind to and transactivate theBcl6andBtlapromoters. Mixed bone marrow chimeras revealed that Bob1 is required for the expression of normal levels of Bcl6 andBTLA, thereby controlling the pool size and composition of the Tfh compartment in a T cell-intrinsic manner. Our data indicate that T cell-expressed Bob1 is directly involved in Tfh cell differentiation and required for mounting normal T cell-dependent B-cell responses.
    MeSH term(s) Animals ; Binding Sites ; CD4-Positive T-Lymphocytes/immunology ; Cell Differentiation ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Female ; Gene Expression Regulation ; Immunization ; Male ; Mice, Inbred C57BL ; Mice, Mutant Strains ; Promoter Regions, Genetic ; Proto-Oncogene Proteins c-bcl-6 ; Receptors, Immunologic/genetics ; Receptors, Immunologic/metabolism ; Receptors, Interleukin-21/genetics ; Receptors, Interleukin-21/metabolism ; Receptors, Interleukin-6/genetics ; Receptors, Interleukin-6/metabolism ; T-Lymphocytes, Helper-Inducer/cytology ; T-Lymphocytes, Helper-Inducer/physiology ; Trans-Activators/genetics ; Trans-Activators/metabolism
    Chemical Substances BTLA protein, mouse ; Bcl6 protein, mouse ; DNA-Binding Proteins ; Pou2af1 protein, mouse ; Proto-Oncogene Proteins c-bcl-6 ; Receptors, Immunologic ; Receptors, Interleukin-21 ; Receptors, Interleukin-6 ; Trans-Activators
    Language English
    Publishing date 2016-03-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 586044-1
    ISSN 1460-2075 ; 0261-4189
    ISSN (online) 1460-2075
    ISSN 0261-4189
    DOI 10.15252/embj.201591459
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1808: Show issues Location:
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    Zs.MG 100: Show issues

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  4. Article ; Online: Dysregulated development of IL-17- and IL-21-expressing follicular helper T cells and increased germinal center formation in the absence of RORγt.

    Wichner, Katharina / Stauss, Dennis / Kampfrath, Branka / Krüger, Kerstin / Müller, Gerd / Rehm, Armin / Lipp, Martin / Höpken, Uta E

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    2016  Volume 30, Issue 2, Page(s) 761–774

    Abstract: Interleukin 17-producing helper T (Th17) cells have been widely defined by the lineage transcription factor retinoid-related orphan receptor (ROR)γt. Pathophysiologically, these cells play a crucial role in autoimmune diseases and have been linked to ... ...

    Abstract Interleukin 17-producing helper T (Th17) cells have been widely defined by the lineage transcription factor retinoid-related orphan receptor (ROR)γt. Pathophysiologically, these cells play a crucial role in autoimmune diseases and have been linked to dysregulated germinal center (GC) reactions and autoantibody production. In this study, we used gene expression and flow cytometric analyses for the characterization of Rorγt(-/-) and Rorγt(-/-)Il21(RFP/+) mice to demonstrate a previously unknown transcriptional flexibility in the development of IL-17-producing Th-cell subsets. We found an accumulation of follicular Th (Tfh) cells by 5.2-fold, spontaneous 13-fold higher GC formation, decreased frequency of follicular Foxp3(+) T-regulatory (Treg) cells (50%), and a 3.4-fold increase in the number of proliferating follicular B cells in RORγt-deficient vs. wild-type mice. Dysregulated B-cell responses were associated with enhanced production of IL-17 (6.4-fold), IL-21 (2.2-fold), and B-cell-activating factor (BAFF) (2-fold) and were partially rescued by adoptive transfer of Treg cells. In an unexpected finding, we detected RORγt-independent IL-17 expression in ICOS(+)CXCR5(+)Tfh and in ICOS(+)CXCR5(-)Th cells. Based on the observed high Irf4 and Batf gene expression, we suggest that CD4(+) T-cell transcription factors other than RORγt can cooperatively induce differentiation of IL-17-producing Th cells, including Th17-like Tfh-cell subsets. We conclude that the occurrence of aberrant Tfh and follicular Treg cells support spontaneous GC formation and dysregulated B-cell responses in RORγt-deficient mice.
    MeSH term(s) Animals ; B-Cell Activating Factor/genetics ; B-Cell Activating Factor/immunology ; B-Lymphocytes/cytology ; B-Lymphocytes/immunology ; Basic-Leucine Zipper Transcription Factors/genetics ; Basic-Leucine Zipper Transcription Factors/immunology ; Cell Differentiation/genetics ; Cell Differentiation/immunology ; Germinal Center/cytology ; Germinal Center/immunology ; Interferon Regulatory Factors/genetics ; Interferon Regulatory Factors/immunology ; Interleukin-17/genetics ; Interleukin-17/immunology ; Interleukins/genetics ; Interleukins/immunology ; Mice ; Mice, Knockout ; Nuclear Receptor Subfamily 1, Group F, Member 3/deficiency ; Nuclear Receptor Subfamily 1, Group F, Member 3/immunology ; T-Lymphocytes, Regulatory/cytology ; T-Lymphocytes, Regulatory/immunology
    Chemical Substances B-Cell Activating Factor ; Basic-Leucine Zipper Transcription Factors ; Batf protein, mouse ; Interferon Regulatory Factors ; Interleukin-17 ; Interleukins ; Nuclear Receptor Subfamily 1, Group F, Member 3 ; Tnfsf13b protein, mouse ; interferon regulatory factor-4 ; interleukin-21
    Language English
    Publishing date 2016-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639186-2
    ISSN 1530-6860 ; 0892-6638
    ISSN (online) 1530-6860
    ISSN 0892-6638
    DOI 10.1096/fj.15-274001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2266: Show issues Location:
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    Zs.MO 296: Show issues

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  5. Article ; Online: Follicular regulatory T cells control humoral autoimmunity via NFAT2-regulated CXCR5 expression.

    Vaeth, Martin / Müller, Gerd / Stauss, Dennis / Dietz, Lena / Klein-Hessling, Stefan / Serfling, Edgar / Lipp, Martin / Berberich, Ingolf / Berberich-Siebelt, Friederike

    The Journal of experimental medicine

    2014  Volume 211, Issue 3, Page(s) 545–561

    Abstract: Maturation of high-affinity B lymphocytes is precisely controlled during the germinal center reaction. This is dependent on CD4(+)CXCR5(+) follicular helper T cells (TFH) and inhibited by CD4(+)CXCR5(+)Foxp3(+) follicular regulatory T cells (TFR). ... ...

    Abstract Maturation of high-affinity B lymphocytes is precisely controlled during the germinal center reaction. This is dependent on CD4(+)CXCR5(+) follicular helper T cells (TFH) and inhibited by CD4(+)CXCR5(+)Foxp3(+) follicular regulatory T cells (TFR). Because NFAT2 was found to be highly expressed and activated in follicular T cells, we addressed its function herein. Unexpectedly, ablation of NFAT2 in T cells caused an augmented GC reaction upon immunization. Consistently, however, TFR cells were clearly reduced in the follicular T cell population due to impaired homing to B cell follicles. This was TFR-intrinsic because only in these cells NFAT2 was essential to up-regulate CXCR5. The physiological relevance for humoral (auto-)immunity was corroborated by exacerbated lupuslike disease in the presence of NFAT2-deficient TFR cells.
    MeSH term(s) Adoptive Transfer ; Analysis of Variance ; Animals ; Autoimmunity/immunology ; Chromatin Immunoprecipitation ; DNA Primers/genetics ; Electrophoretic Mobility Shift Assay ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Fluorescent Antibody Technique ; Gene Expression Profiling ; Germinal Center/immunology ; Immunity, Humoral/immunology ; Immunohistochemistry ; Mice ; Mice, Knockout ; NFATC Transcription Factors/genetics ; NFATC Transcription Factors/immunology ; NFATC Transcription Factors/metabolism ; Real-Time Polymerase Chain Reaction ; Receptors, CXCR5/immunology ; Receptors, CXCR5/metabolism ; T-Lymphocytes, Regulatory/immunology
    Chemical Substances CXCR5 protein, mouse ; DNA Primers ; NFATC Transcription Factors ; Nfatc1 protein, mouse ; Receptors, CXCR5
    Language English
    Publishing date 2014-03-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218343-2
    ISSN 1540-9538 ; 0022-1007
    ISSN (online) 1540-9538
    ISSN 0022-1007
    DOI 10.1084/jem.20130604
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Ua VI Zs.107: Show issues Location:
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    Zs.MG 45: Show issues

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  6. Article: Identification of specific genes in Staphylococcus aureus strains associated with bovine mastitis

    Kozytska, Svitlana / Stauß, Dennis / Pawlik, Marie-Christin / Hensen, Selma / Eckart, Martin / Ziebuhr, Wilma / Witte, Wolfgang / Ohlsen, Knut

    Veterinary microbiology. 2010 Oct. 26, v. 145, no. 3-4

    2010  

    Abstract: Staphylococcus aureus is a common cause of bovine mastitis that is responsible for the main economic loss to the dairy industry. For identification of putative, bovine-specific molecular marker a genome comparison between bovine S. aureus strain RF122 ... ...

    Abstract Staphylococcus aureus is a common cause of bovine mastitis that is responsible for the main economic loss to the dairy industry. For identification of putative, bovine-specific molecular marker a genome comparison between bovine S. aureus strain RF122 and 52 previously sequenced S. aureus isolates associated with human infections using genome viewer, annotation tool Artemis Comparison Tool (ACT), KEGG and NCBI BLAST databases was carried out. This led to the identification of 16 unique RF122 gene sequences that may be used as molecular marker to distinguish bovine from human strains. The distribution of these genes was analyzed in a collection of bovine mastitis strains from the Netherlands and human clinical isolates by PCR and Southern blotting. Only four genes within the pathogenicity island SaPIbov3 (sab1890, sab1891, sab1892, sab1893) were present in the majority of isolates from cattle but were absent from human clinical S. aureus isolates. These results suggest that there is no gene/ORF uniformly shared by all bovine S. aureus strains that could be uniformly used as a diagnostic marker gene.
    Keywords dairy cows ; bovine mastitis ; Staphylococcus aureus ; bacterial infections ; strains ; phenotype ; genotype ; genetic variation ; microbial genetics ; genome ; polymerase chain reaction ; Southern blotting ; pathogenicity ; open reading frames ; Netherlands
    Language English
    Dates of publication 2010-1026
    Size p. 360-365.
    Publishing place Amsterdam; New York: Elsevier
    Document type Article
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2010.03.020
    Database NAL-Catalogue (AGRICOLA)

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    Zs.A 2917: Show issues Location:
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  7. Article ; Online: Identification of specific genes in Staphylococcus aureus strains associated with bovine mastitis.

    Kozytska, Svitlana / Stauss, Dennis / Pawlik, Marie-Christin / Hensen, Selma / Eckart, Martin / Ziebuhr, Wilma / Witte, Wolfgang / Ohlsen, Knut

    Veterinary microbiology

    2010  Volume 145, Issue 3-4, Page(s) 360–365

    Abstract: Staphylococcus aureus is a common cause of bovine mastitis that is responsible for the main economic loss to the dairy industry. For identification of putative, bovine-specific molecular marker a genome comparison between bovine S. aureus strain RF122 ... ...

    Abstract Staphylococcus aureus is a common cause of bovine mastitis that is responsible for the main economic loss to the dairy industry. For identification of putative, bovine-specific molecular marker a genome comparison between bovine S. aureus strain RF122 and 52 previously sequenced S. aureus isolates associated with human infections using genome viewer, annotation tool Artemis Comparison Tool (ACT), KEGG and NCBI BLAST databases was carried out. This led to the identification of 16 unique RF122 gene sequences that may be used as molecular marker to distinguish bovine from human strains. The distribution of these genes was analyzed in a collection of bovine mastitis strains from the Netherlands and human clinical isolates by PCR and Southern blotting. Only four genes within the pathogenicity island SaPIbov3 (sab1890, sab1891, sab1892, sab1893) were present in the majority of isolates from cattle but were absent from human clinical S. aureus isolates. These results suggest that there is no gene/ORF uniformly shared by all bovine S. aureus strains that could be uniformly used as a diagnostic marker gene.
    MeSH term(s) Animals ; Cattle ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Electrophoresis, Gel, Pulsed-Field/veterinary ; Female ; Genome, Bacterial ; Genomic Islands/genetics ; Humans ; Mastitis, Bovine/microbiology ; Netherlands ; Polymerase Chain Reaction/veterinary ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/veterinary ; Staphylococcus aureus/genetics ; Staphylococcus aureus/pathogenicity
    Chemical Substances DNA, Bacterial
    Language English
    Publishing date 2010-10-26
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2010.03.020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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