LIVIVO - Search results -

Search results

Result 1 - 10 of total 605

Search options

Article ; Online: Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy.

Ghoneim, Mohamed / Musselman, Catherine A

STAR protocols

2023 Volume 4, Issue 2, Page(s) 102229

Abstract: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing doubly ... ...

Abstract	Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing doubly labeled fluorescent nucleosomes for SMFM. We describe steps for over-expression in E. coli and purification of recombinant human core histones. We then detail fluorescent labeling of histones and nucleosomal double-stranded DNA followed by octamer refolding and nucleosome reconstitution.
Language	English
Publishing date	2023-04-19
Publishing country	United States
Document type	Journal Article
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2023.102229
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy

Mohamed Ghoneim / Catherine A. Musselman

STAR Protocols, Vol 4, Iss 2, Pp 102229- (2023)

2023

Abstract: Summary: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing ... ...

Abstract	Summary: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing doubly labeled fluorescent nucleosomes for SMFM. We describe steps for over-expression in E. coli and purification of recombinant human core histones. We then detail fluorescent labeling of histones and nucleosomal double-stranded DNA followed by octamer refolding and nucleosome reconstitution. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Keywords	Biophysics ; Single-molecule Assays ; Microscopy ; Molecular Biology ; Molecular/Chemical Probes ; Protein Biochemistry ; Science (General) ; Q1-390
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Single-Molecule Characterization of Cy3.5 -Cy5.5 Dye Pair for FRET Studies of Nucleic Acids and Nucleosomes.

Ghoneim, Mohamed / Musselman, Catherine A

Journal of fluorescence

2022 Volume 33, Issue 2, Page(s) 413–421

Abstract: Single molecule FRET (Forster resonance energy transfer) is very powerful method for studying biomolecular binding dynamics and conformational transitions. Only a few donor - acceptor dye pairs have been characterized for use in single-molecule FRET ( ... ...

Abstract	Single molecule FRET (Forster resonance energy transfer) is very powerful method for studying biomolecular binding dynamics and conformational transitions. Only a few donor - acceptor dye pairs have been characterized for use in single-molecule FRET (smFRET) studies. Hence, introducing and characterizing additional FRET dye pairs is important in order to widen the scope of applications of single-molecule FRET in biomolecular studies. Here we characterize the properties of the Cy3.5 and Cy5.5 dye pair under FRET at the single-molecule level using naked double-stranded DNA (dsDNA) and the nucleosome. We show that this pair of dyes is photostable for ~ 5 min under continuous illumination. We also report Cy3.5-Cy5.5 FRET proximity dependence and stability in the presence of several biochemical buffers and photoprotective reagents in the context of double-stranded DNA. Finally, we demonstrate compatibility of the Cy3.5-Cy5.5 pair for smFRET in vitro studies of nucleosomes.
MeSH term(s)	Fluorescence Resonance Energy Transfer/methods ; Nucleic Acids ; Nucleosomes ; Carbocyanines/chemistry ; DNA/chemistry ; Coloring Agents ; Fluorescent Dyes/chemistry
Chemical Substances	Nucleic Acids ; Nucleosomes ; cyanine dye 3 ; Carbocyanines ; DNA (9007-49-2) ; Coloring Agents ; Fluorescent Dyes
Language	English
Publishing date	2022-11-26
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2016892-5
ISSN	1573-4994 ; 1053-0509
ISSN (online)	1573-4994
ISSN	1053-0509
DOI	10.1007/s10895-022-03093-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Evaluation of postoperative pain and healing following regenerative endodontics using platelet-rich plasma versus conventional endodontic treatment in necrotic mature mandibular molars with chronic periapical periodontitis. A randomized clinical trial.

Ahmed, Yassmin Elsayed / Ahmed, Geraldine Mohamed / Ghoneim, Angie Galal

International endodontic journal

2023 Volume 56, Issue 4, Page(s) 404–418

Abstract: Aim: To evaluate the post-operative pain and the healing of necrotic mature permanent mandibular molar teeth with peri-apical periodontitis after conventional endodontic treatment versus after platelet-rich plasma (PRP) revascularization.: Methodology! ...

Abstract	Aim: To evaluate the post-operative pain and the healing of necrotic mature permanent mandibular molar teeth with peri-apical periodontitis after conventional endodontic treatment versus after platelet-rich plasma (PRP) revascularization. Methodology: The protocol of this randomized clinical trial was registered at www. Clinicaltrials: gov with identification number NCT03350841. Twenty-eight patients were included in the study after confirming the diagnosis clinically and radiographically. In the first visit, mandibular molar teeth of both groups were mechanically prepared. Double antibiotic paste was prepared and injected then the cavity that was sealed with glass ionomer. At the second visit, the patients were randomized either to control group where standard endodontic treatment was completed by lateral condensation technique or assigned to intervention group where PRP revascularization technique. The degree of spontaneous pain was assessed using numerical rating scale (NRS) pre-operatively, then post-operatively after 6, 12 h and daily for 5 days. Patients were given a placebo to be administrated in case of pain. An analgesic (ibuprofen 400mg) was prescribed in case of persistent pain. Clinical and radiographic healing was assessed after 6 and 12 months. All demographic, baseline and outcome data were collected and statistically analysed. Results: Regarding the post revascularization/obturation pain, there was no statistically significant difference between severity of pain in the two groups at all time intervals except after 12 h, revascularization group showed statistically significantly higher prevalence of no pain than endodontic treatment group. Regarding the healing; the periapical lesions decrease in size significantly from the pre-operative lesion size in both groups without significant difference between the both groups. Conclusion: Within the limitation of this study, PRP revascularization could be an alternative treatment to root canal treatment but further randomized clinical trials with standardized techniques and long follow up periods are recommended for more reliable results.
MeSH term(s)	Humans ; Regenerative Endodontics ; Dental Pulp Necrosis/therapy ; Root Canal Therapy/methods ; Necrosis ; Periapical Periodontitis/therapy ; Platelet-Rich Plasma ; Pain, Postoperative ; Molar
Language	English
Publishing date	2023-01-12
Publishing country	England
Document type	Randomized Controlled Trial ; Journal Article
ZDB-ID	603734-3
ISSN	1365-2591 ; 0143-2885
ISSN (online)	1365-2591
ISSN	0143-2885
DOI	10.1111/iej.13886
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 1508: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Assessment of maxillary sinus lifting procedure in the presence of chronic sinusitis, a retrospective comparative study.

Hammuda, Abdullah Atef / Ghoneim, Mohamed Moawad

Annals of medicine and surgery (2012)

2021 Volume 66, Page(s) 102379

Abstract: Introduction: Chronic sinusitis can be considered a relative contraindication for sinus lifting procedure. However, its specific effects on bone height, infection and healing have not been as well investigated as its incidence as a post-operative ... ...

Abstract	Introduction: Chronic sinusitis can be considered a relative contraindication for sinus lifting procedure. However, its specific effects on bone height, infection and healing have not been as well investigated as its incidence as a post-operative complication. Methods: A retrospective comparative investigation was executed to evaluate the impact of chronic sinusitis on sinus lifting procedures regarding bone height, infection, and healing scores. Pre-operative and 6-month postoperative records (CBCT or CT and panoramic radiographs) of 40 patients who underwent sinus lifting procedure with graft and implant placement were split into two sets of 20 patients each; Group A is comprised of a patient with healthy sinus, whereas Group B includes patients with chronic sinusitis (identified as the presence of thickening of Schniederian membrane ≥ 2 mm, mucosal cyst, polyp or fluid level). Records of both groups were assessed for the difference in bone height of alveolar ridge between pre-operative radiograph and after six months postoperatively, and clinical notifications at the postoperative follow-up to report the healing and infection scores. Results: Statistically, there was non-significant difference in mean bone height gain ( Conclusion: According to the available data, chronic sinusitis presenting as a thickening in the Schneiderian membrane has no significant effect on postoperative bone height, healing, or infection score in patients undergoing sinus augmentation with simultaneous implant placement. Further research is needed to better evaluate the effect of chronic sinusitis and its current status as a relative contraindication for sinus lifting procedure.
Language	English
Publishing date	2021-05-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2745440-X
ISSN	2049-0801
ISSN	2049-0801
DOI	10.1016/j.amsu.2021.102379
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Effect of Die Spacer Thickness on the Microshear Bond Strength of CAD/CAM Lithium Disilicate Veneers.

Farag, Sherine Mohamed / Ghoneim, Mona Mohamed / Afifi, Rania Reda

International journal of dentistry

2021 Volume 2021, Page(s) 4593131

Abstract: Aim: The aim of this study was to compare the microshear bond strength of ceramic veneers with digital die spacer settings at 20, 40, and 100 : Materials and methods: Eighteen milled lithium disilicate microdiscs (IPS e.max CAD, Ivoclar Vivadent) ... ...

Abstract	Aim: The aim of this study was to compare the microshear bond strength of ceramic veneers with digital die spacer settings at 20, 40, and 100 Materials and methods: Eighteen milled lithium disilicate microdiscs (IPS e.max CAD, Ivoclar Vivadent) were divided into three groups ( Results: The mean microshear bond strength values were calculated in MPa for group A = 31.91 ± 12.41, group B = 29.58 ± 5.03, and group C = 13.85 ± 4.12. One-way ANOVA ( Conclusion: Higher digital die spacer settings decrease the microshear bond strength of CAD/CAM lithium disilicate veneers.
Language	English
Publishing date	2021-07-23
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2546524-7
ISSN	1687-8736 ; 1687-8728
ISSN (online)	1687-8736
ISSN	1687-8728
DOI	10.1155/2021/4593131
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Current status of stem cell therapy for type 1 diabetes: a critique and a prospective consideration.

Ghoneim, Mohamed A / Gabr, Mahmoud M / El-Halawani, Sawsan M / Refaie, Ayman F

Stem cell research & therapy

2024 Volume 15, Issue 1, Page(s) 23

Abstract: Over the past decade, there had been progress in the development of cell therapy for insulin-dependent diabetes. Nevertheless, important hurdles that need to be overcome still remain. Protocols for the differentiation of pluripotent stem cells into ... ...

Abstract	Over the past decade, there had been progress in the development of cell therapy for insulin-dependent diabetes. Nevertheless, important hurdles that need to be overcome still remain. Protocols for the differentiation of pluripotent stem cells into pancreatic progenitors or fully differentiated β-cells have been developed. The resulting insulin-producing cells can control chemically induced diabetes in rodents and were the subject of several clinical trials. However, these cells are immunogenic and possibly teratogenic for their transplantation, and an immunoisolation device and/or immunosuppression is needed. A growing number of studies have utilized genetic manipulations to produce immune evasive cells. Evidence must be provided that in addition to the expected benefit, gene manipulations should not lead to any unforeseen complications. Mesenchymal stem/stromal cells (MSCs) can provide a viable alternative. MSCs are widely available from many tissues. They can form insulin-producing cells by directed differentiation. Experimentally, evidence has shown that the transplantation of allogenic insulin-producing cells derived from MSCs is associated with a muted allogeneic response that does not interfere with their functionality. This can be explained by the immunomodulatory functions of the MSC subpopulation that did not differentiate into insulin-producing cells. Recently, exosomes derived from naive MSCs have been used in the experimental domain to treat diabetes in rodents with varying degrees of success. Several mechanisms for their beneficial functions were proposed including a reduction in insulin resistance, the promotion of autophagy, and an increase in the T regulatory population. However, euglycemia was not achieved in any of these experiments. We suggest that exosomes derived from β-cells or insulin-producing cells (educated) can provide a better therapeutic effect than those derived from undifferentiated cells.
MeSH term(s)	Humans ; Diabetes Mellitus, Type 1/therapy ; Diabetes Mellitus, Type 1/metabolism ; Prospective Studies ; Mesenchymal Stem Cell Transplantation/methods ; Insulin-Secreting Cells ; Cell Differentiation ; Insulin/metabolism
Chemical Substances	Insulin
Language	English
Publishing date	2024-01-29
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-024-03636-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Occurrence, distribution, and composition of black sand along the Red Sea, Egypt.

Hamed, Mohamed / El-Aal, Mohamed Abd / Khaled, Mostafa A / Ghoneim, Sobhi M / Saad, Eman / Lee, Jae-Seong / Sayed, Alaa El-Din H

The Science of the total environment

2024 Volume 923, Page(s) 171277

Abstract: Black sand along the Red Sea is often composed of volcanic minerals and heavy minerals. The Red Sea region is known for its unique geological features, and black sand beaches can be found in various areas along its shores. The study presents a ... ...

Abstract	Black sand along the Red Sea is often composed of volcanic minerals and heavy minerals. The Red Sea region is known for its unique geological features, and black sand beaches can be found in various areas along its shores. The study presents a comprehensive semi-quantitative chemical analysis of black sand samples collected from various locations along the red sea, revealing significant variations in their elemental compositions. The main oxides were identified in each sample, determined through X-ray diffraction (XRD) and X-ray fluorescence (XRF) analyses, indicate diverse mineralogical compositions. The spatial distribution of minerals at each site is depicted through mapping. Additionally, Fourier-transform infrared (FTIR) spectra offer information on the functional groups present in the samples, revealing the existence of hydroxyl groups, aliphatic compounds, and adsorbed water molecules. For Qusier-Elsharm Alqbly, Safaga, Marsa Alam, Gabal Alrosass, Hurghada Titanic, Hurghada Elahiaa, Gemsa, and Ras Elbehar samples, the results highlight the presence of various minerals, such as Quartz, Calcite, Titanium Dioxide, Magnetite, Hematite, Aluminum Oxide, Zirconium Dioxide, Chromium (III) Oxide, and others, providing insights into the geological characteristics of each location. The differences in mineral content among the examined sites are linked to the geological and mineralogical makeup of the source rocks upstream and midstream in the basins that discharge into the surveyed regions. So, variations in black sand concentrations among different locations offer insights into the geological and mineralogical diversity of the studied areas along the Red Sea coast. This study addresses the existing knowledge gap by focusing on the preliminary exploration and description of the occurrence, distribution, and composition of black sand along the Red Sea in Egypt. whereas the results provide valuable insights into the geological diversity of black sand deposits in the surveyed areas, underscoring the need for additional research and interpretation of these variations. Therefore, the in-depth examination of mineralogical composition and crystal structures establishes a foundation for future investigations in the field of geology and earth sciences.
Language	English
Publishing date	2024-02-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.171277
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 949: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Book: Awareness during anesthesia

Ghoneim, Mohamed M.

2001

Author's details	ed. by Mohamed M. Ghoneim
Language	English
Size	XXII, 181 S. : graph. Darst.
Publisher	Butterworth Heinemann
Publishing place	Oxford u.a.
Publishing country	Great Britain
Document type	Book
HBZ-ID	HT012848381
ISBN	0-7506-7201-3 ; 978-0-7506-7201-6
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

Shelf mark	Loan status	Location
2000 A 4598	order for loan	Magazin

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Endoscopic third ventriculostomy for management of hydrocephalus associated with Chiari malformation type II in children.

El-Ghandour, Nasser M F / Salama, Mohamed M / Ghoneim, Mohamed Adel / Attia, Ahmed M

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery

2023 Volume 39, Issue 6, Page(s) 1565–1571

Abstract: Background: Hydrocephalus is commonly associated with Chiari malformation (CM) particularly CM type II. The traditional treatment of hydrocephalus in these patients has been cerebrospinal fluid diversion by shunts. Endoscopic third ventriculostomy (ETV) ...

Abstract	Background: Hydrocephalus is commonly associated with Chiari malformation (CM) particularly CM type II. The traditional treatment of hydrocephalus in these patients has been cerebrospinal fluid diversion by shunts. Endoscopic third ventriculostomy (ETV) has emerged as an alternative procedure in these patients. Purpose: Assessment of the clinical and radiological outcomes of ETV in the management of hydrocephalus in children with CM II. Methods: This is a prospective study conducted on 18 patients with CM II associated with hydrocephalus admitted to Cairo University hospitals between January 2020 and June 2021. These patients had been managed surgically by ETV. Clinical outcome was assessed based on improvement of manifestations of increased intracranial pressure while radiological outcome was based on the findings of postoperative computed tomography. In cases with early failure, serial lumbar puncture (LP) was performed for 2 days. Results: ETV was performed as a secondary procedure in 4 cases. The overall success rate of the procedure was 72%, and its success rate as a secondary procedure was 100%. Serial LP was effective in decreasing early failure in 44.4% of cases. Radiological regression of hydrocephalic changes was detected in 50% of the cases. Conclusion: ETV is an efficient and safe procedure in the treatment of hydrocephalus in children with Chiari malformation II, particularly when performed as a secondary procedure. Serial LP following the procedure increases the success rate in patients with early failure.
MeSH term(s)	Humans ; Child ; Arnold-Chiari Malformation/complications ; Arnold-Chiari Malformation/diagnostic imaging ; Arnold-Chiari Malformation/surgery ; Ventriculostomy/methods ; Prospective Studies ; Third Ventricle/diagnostic imaging ; Third Ventricle/surgery ; Treatment Outcome ; Neuroendoscopy/methods ; Hydrocephalus/diagnostic imaging ; Hydrocephalus/etiology ; Hydrocephalus/surgery ; Retrospective Studies
Language	English
Publishing date	2023-01-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	605988-0
ISSN	1433-0350 ; 0302-2803 ; 0256-7040
ISSN (online)	1433-0350
ISSN	0302-2803 ; 0256-7040
DOI	10.1007/s00381-023-05832-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2036: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito