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  1. Article ; Online: B-cell ELISpot assay to analyze human memory B cell and plasmablast responses specific to SARS-CoV-2 receptor-binding domain.

    Rouers, Angeline / Tay, Matthew Zirui / Ng, Lisa F P / Renia, Laurent

    STAR protocols

    2023  Volume 4, Issue 1, Page(s) 102130

    Abstract: ... details on the use and execution of this protocol, please refer to Tay et al. (2022). ...

    Abstract B-cell ELISpot is an extremely sensitive assay based on the secretion of antibodies by B cells that requires the differentiation of B cells into antibody-secreting cells. Here, we describe the procedure to analyze both plasmablast (PB) and memory B cell (MBC) responses specific to SARS-CoV-2 receptor-binding domain (RBD) in the context of acute SARS-CoV-2 infection and vaccination. We detail steps for MBC stimulation, MBC and PB plating, detection, and counting of total IgG and RBD-specific spots. For complete details on the use and execution of this protocol, please refer to Tay et al. (2022).
    MeSH term(s) Humans ; Memory B Cells ; SARS-CoV-2 ; COVID-19 ; B-Lymphocytes ; Plasma Cells
    Language English
    Publishing date 2023-02-09
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S. ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2023.102130
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: B-cell ELISpot assay to analyze human memory B cell and plasmablast responses specific to SARS-CoV-2 receptor-binding domain

    Angeline Rouers / Matthew Zirui Tay / Lisa F.P. Ng / Laurent Renia

    STAR Protocols, Vol 4, Iss 1, Pp 102130- (2023)

    2023  

    Abstract: ... details on the use and execution of this protocol, please refer to Tay et al. (2022).1 : Publisher’s note ...

    Abstract Summary: B-cell ELISpot is an extremely sensitive assay based on the secretion of antibodies by B cells that requires the differentiation of B cells into antibody-secreting cells. Here, we describe the procedure to analyze both plasmablast (PB) and memory B cell (MBC) responses specific to SARS-CoV-2 receptor-binding domain (RBD) in the context of acute SARS-CoV-2 infection and vaccination. We detail steps for MBC stimulation, MBC and PB plating, detection, and counting of total IgG and RBD-specific spots.For complete details on the use and execution of this protocol, please refer to Tay et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
    Keywords Cell Isolation ; Cell-based Assays ; Health Sciences ; Immunology ; Antibody ; Science (General) ; Q1-390
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Antibody-Dependent Cellular Phagocytosis in Antiviral Immune Responses.

    Tay, Matthew Zirui / Wiehe, Kevin / Pollara, Justin

    Frontiers in immunology

    2019  Volume 10, Page(s) 332

    Abstract: Antiviral activities of antibodies may either be dependent only on interactions between the antibody and cognate antigen, as in binding and neutralization of an infectious virion, or instead may require interactions between antibody-antigen immune ... ...

    Abstract Antiviral activities of antibodies may either be dependent only on interactions between the antibody and cognate antigen, as in binding and neutralization of an infectious virion, or instead may require interactions between antibody-antigen immune complexes and immunoproteins or Fc receptor expressing immune effector cells. These Fc receptor-dependent antibody functions provide a direct link between the innate and adaptive immune systems by combining the potent antiviral activity of innate effector cells with the diversity and specificity of the adaptive humoral response. The Fc receptor-dependent function of antibody-dependent cellular phagocytosis (ADCP) provides mechanisms for clearance of virus and virus-infected cells, as well as for stimulation of downstream adaptive immune responses by facilitating antigen presentation, or by stimulating the secretion of inflammatory mediators. In this review, we discuss the properties of Fc receptors, antibodies, and effector cells that influence ADCP. We also provide and interpret evidence from studies that support a potential role for ADCP in either inhibiting or enhancing viral infection. Finally, we describe current approaches used to measure antiviral ADCP and discuss considerations for the translation of studies performed in animal models. We propose that additional investigation into the role of ADCP in protective viral responses, the specific virus epitopes targeted by ADCP antibodies, and the types of phagocytes and Fc receptors involved in ADCP at sites of virus infection will provide insight into strategies to successfully leverage this important immune response for improved antiviral immunity through rational vaccine design.
    MeSH term(s) Animals ; Antibodies, Viral/immunology ; Antibody-Dependent Cell Cytotoxicity ; Epitopes/immunology ; Humans ; Phagocytes/immunology ; Phagocytosis ; Receptors, Fc/immunology ; Virus Diseases/immunology
    Chemical Substances Antibodies, Viral ; Epitopes ; Receptors, Fc
    Keywords covid19
    Language English
    Publishing date 2019-02-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2019.00332
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  4. Article ; Online: Functional and immunological mapping of domains of the reticulocyte binding protein, Plasmodium vivax PvRBP2a.

    Tay, Matthew Zirui / Tang, Weiyi / Lee, Wenn-Chyau / Ong, Alice Soh Meoy / Novera, Wisna / Malleret, Benoît / Carissimo, Guillaume / Chacko, Ann-Marie / El-Sahili, Abbas / Lescar, Julien / Fan, Yiping / McGready, Rose M / Chu, Cindy S / Chan, Jerry Kok Yen / Ng, Lisa F P / Russell, Bruce / Nosten, François / Rénia, Laurent

    The Journal of infectious diseases

    2024  

    Abstract: We previously described a novel Plasmodium vivax invasion mechanism into human reticulocytes via the PvRBP2a-CD98 receptor-ligand pair. We assessed the PvRBP2a epitopes involved in CD98 binding and recognised by antibodies from infected patients using ... ...

    Abstract We previously described a novel Plasmodium vivax invasion mechanism into human reticulocytes via the PvRBP2a-CD98 receptor-ligand pair. We assessed the PvRBP2a epitopes involved in CD98 binding and recognised by antibodies from infected patients using linear epitope mapping. We identified two epitope clusters mediating PvRBP2a-CD98 interaction. One cluster named cluster B (PvRBP2a431-448, TAALKEKGKLLANLYNKL) was the target of antibody responses in P. vivax-infected humans. Peptides from each cluster were able to prevent live parasite invasion of human reticulocytes. These results provide new insights for development of a malaria blood stage vaccine against P. vivax.
    Language English
    Publishing date 2024-03-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiae111
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  5. Article ; Online: Author Correction: Higher Delta variant-specific neutralizing antibodies prevented infection in close contacts vaccinated with ancestral mRNA vaccines during the SARS-CoV-2 Delta wave.

    Goh, Yun Shan / Fong, Siew-Wai / Tay, Matthew Zirui / Rouers, Angeline / Chang, Zi Wei / Chavatte, Jean-Marc / Hor, Pei Xiang / Loh, Chiew Yee / Huang, Yuling / Tan, Yong Jie / Wang, Bei / Ngoh, Eve Zi Xian / Mohd Salleh, Siti Nazihah / Lee, Raphael Tze Chuen / Lim, Georgina / Maurer-Stroh, Sebastian / Wang, Cheng-I / Leo, Yee-Sin / Lin, Raymond T P /
    Lam, Meng Chon / Lye, David C / Young, Barnaby Edward / Ng, Lisa F P / Renia, Laurent

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 1492

    Language English
    Publishing date 2024-01-17
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-51484-y
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  6. Article ; Online: The Role of Single-Cell Technology in the Study and Control of Infectious Diseases.

    Lin, Weikang Nicholas / Tay, Matthew Zirui / Lu, Ri / Liu, Yi / Chen, Chia-Hung / Cheow, Lih Feng

    Cells

    2020  Volume 9, Issue 6

    Abstract: The advent of single-cell research in the recent decade has allowed biological studies at an unprecedented resolution and scale. In particular, single-cell analysis techniques such as Next-Generation Sequencing (NGS) and Fluorescence-Activated Cell ... ...

    Abstract The advent of single-cell research in the recent decade has allowed biological studies at an unprecedented resolution and scale. In particular, single-cell analysis techniques such as Next-Generation Sequencing (NGS) and Fluorescence-Activated Cell Sorting (FACS) have helped show substantial links between cellular heterogeneity and infectious disease progression. The extensive characterization of genomic and phenotypic biomarkers, in addition to host-pathogen interactions at the single-cell level, has resulted in the discovery of previously unknown infection mechanisms as well as potential treatment options. In this article, we review the various single-cell technologies and their applications in the ongoing fight against infectious diseases, as well as discuss the potential opportunities for future development.
    MeSH term(s) Communicable Diseases/therapy ; Humans ; Single-Cell Analysis/methods
    Keywords covid19
    Language English
    Publishing date 2020-06-10
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells9061440
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  7. Article ; Online: The trinity of COVID-19

    Tay, Matthew Zirui / Poh, Chek Meng / Rénia, Laurent / MacAry, Paul A. / Ng, Lisa F. P.

    Nature Reviews Immunology

    immunity, inflammation and intervention

    2020  Volume 20, Issue 6, Page(s) 363–374

    Keywords Immunology ; covid19
    Language English
    Publisher Springer Science and Business Media LLC
    Publishing country us
    Document type Article ; Online
    ZDB-ID 2062776-2
    ISSN 1474-1741 ; 1474-1733
    ISSN (online) 1474-1741
    ISSN 1474-1733
    DOI 10.1038/s41577-020-0311-8
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5565: Show issues Location:
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  8. Article ; Online: Rapid microfluidic platform for screening and enrichment of cells secreting virus neutralizing antibodies.

    Lin, Weikang Nicholas / Tay, Matthew Zirui / Wong, Joel Xu En / Lee, Chia Yin / Fong, Siew-Wai / Wang, Cheng-I / Ng, Lisa Fong Poh / Renia, Laurent / Chen, Chia-Hung / Cheow, Lih Feng

    Lab on a chip

    2022  Volume 22, Issue 13, Page(s) 2578–2589

    Abstract: As part of the body's immune response, antibodies (Abs) have the ability to neutralize pathogenic viruses to prevent infection. To screen for neutralizing Abs (nAbs) from the immune repertoire, multiple screening techniques have been developed. However, ... ...

    Abstract As part of the body's immune response, antibodies (Abs) have the ability to neutralize pathogenic viruses to prevent infection. To screen for neutralizing Abs (nAbs) from the immune repertoire, multiple screening techniques have been developed. However, conventional methods have a trade-off between screening throughput and the ability to screen for nAbs
    MeSH term(s) Antibodies, Neutralizing ; Antibodies, Viral ; Microfluidics
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral
    Language English
    Publishing date 2022-06-28
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d2lc00018k
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  9. Article ; Online: The trinity of COVID-19: immunity, inflammation and intervention.

    Tay, Matthew Zirui / Poh, Chek Meng / Rénia, Laurent / MacAry, Paul A / Ng, Lisa F P

    Nature reviews. Immunology

    2020  Volume 20, Issue 6, Page(s) 363–374

    Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Alongside investigations into the virology of SARS-CoV-2, understanding the fundamental physiological and ... ...

    Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Alongside investigations into the virology of SARS-CoV-2, understanding the fundamental physiological and immunological processes underlying the clinical manifestations of COVID-19 is vital for the identification and rational design of effective therapies. Here, we provide an overview of the pathophysiology of SARS-CoV-2 infection. We describe the interaction of SARS-CoV-2 with the immune system and the subsequent contribution of dysfunctional immune responses to disease progression. From nascent reports describing SARS-CoV-2, we make inferences on the basis of the parallel pathophysiological and immunological features of the other human coronaviruses targeting the lower respiratory tract - severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV). Finally, we highlight the implications of these approaches for potential therapeutic interventions that target viral infection and/or immunoregulation.
    MeSH term(s) Animals ; Betacoronavirus/immunology ; Betacoronavirus/physiology ; COVID-19 ; Coronavirus Infections/immunology ; Coronavirus Infections/physiopathology ; Coronavirus Infections/therapy ; Coronavirus Infections/virology ; Disease Progression ; Humans ; Inflammation/etiology ; Inflammation/immunology ; Pandemics ; Pneumonia, Viral/immunology ; Pneumonia, Viral/physiopathology ; Pneumonia, Viral/therapy ; Pneumonia, Viral/virology ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-04-28
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2062776-2
    ISSN 1474-1741 ; 1474-1733
    ISSN (online) 1474-1741
    ISSN 1474-1733
    DOI 10.1038/s41577-020-0311-8
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  10. Article ; Online: Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells.

    Torres-Ruesta, Anthony / Teo, Teck-Hui / Chan, Yi-Hao / Amrun, Siti Naqiah / Yeo, Nicholas Kim-Wah / Lee, Cheryl Yi-Pin / Nguee, Samantha Yee-Teng / Tay, Matthew Zirui / Nosten, Francois / Fong, Siew-Wai / Lum, Fok-Moon / Carissimo, Guillaume / Renia, Laurent / Ng, Lisa Fp

    Life science alliance

    2022  Volume 5, Issue 4

    Abstract: O'nyongnyong virus (ONNV) is a re-emerging alphavirus previously known to be transmitted by main malaria vectors, thus suggesting the possibility of coinfections with arboviruses in co-endemic areas. However, the pathological outcomes of such infections ... ...

    Abstract O'nyongnyong virus (ONNV) is a re-emerging alphavirus previously known to be transmitted by main malaria vectors, thus suggesting the possibility of coinfections with arboviruses in co-endemic areas. However, the pathological outcomes of such infections remain unknown. Using murine coinfection models, we demonstrated that a preexisting blood-stage
    MeSH term(s) Alphavirus Infections ; Animals ; Cell Line ; Coinfection/parasitology ; Coinfection/virology ; Disease Models, Animal ; Host-Pathogen Interactions ; Malaria ; Mice ; Microbial Interactions ; O'nyong-nyong Virus/pathogenicity
    Language English
    Publishing date 2022-01-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2575-1077
    ISSN (online) 2575-1077
    DOI 10.26508/lsa.202101272
    Database MEDical Literature Analysis and Retrieval System OnLINE

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