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Article: Acceptance and needs of medication literacy education among children by their caregivers: A multicenter study in mainland China.

Xu, Xiaolin / Wang, Zhonglan / Li, Xialei / Li, Ying / Wang, Yu / Wu, Xuexin / Hao, Lina / Wang, Xiaoling

2022 Volume 13, Page(s) 963251

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-09-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.963251
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Article ; Online: Cell population data in identifying active tuberculosis and community-acquired pneumonia

Sun Tingting / Wu Bin / Luo Zhonglan / Wang Jing / Deng Shaoli / Huang Qing

Open Medicine, Vol 16, Iss 1, Pp 1143-

2021 Volume 1149

Abstract: Leukocyte morphological parameters known as CPD (cell population data) is detected by hematology analyzer UniCel DxH800 with VCS technology. This study aimed to investigate the diagnostic efficacy of morphological changes in CPD parameters in ... ...

Abstract	Leukocyte morphological parameters known as CPD (cell population data) is detected by hematology analyzer UniCel DxH800 with VCS technology. This study aimed to investigate the diagnostic efficacy of morphological changes in CPD parameters in distinguishing active tuberculosis from community-acquired pneumonia.
Keywords	active tuberculosis ; cell population data ; community-acquired pneumonia ; vcs technology ; cpd parameters ; Medicine ; R
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	De Gruyter
Document type	Article ; Online
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Article ; Online: De novo transcriptomic analysis and identification of EST-SSR markers in Stephanandra incisa

Cuiping Zhang / Zhonglan Wu / Xinqiang Jiang / Wei Li / Yizeng Lu / Kuiling Wang

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 10

Abstract: Abstract Stephanandra incisa is a wild-type shrub with beautiful leaves and white flowers and is commonly used as a garden decoration accessory. However, the limited availability of genomic data of S. incisa has restricted its breeding process. Here, we ... ...

Abstract	Abstract Stephanandra incisa is a wild-type shrub with beautiful leaves and white flowers and is commonly used as a garden decoration accessory. However, the limited availability of genomic data of S. incisa has restricted its breeding process. Here, we identified EST-SSR markers using de novo transcriptome sequencing. In this study, a transcriptome database containing 35,251 unigenes, having an average length of 985 bp, was obtained from S. incisa. From these unigene sequences, we identified 5,555 EST-SSRs, with a distribution density of one SSR per 1.60 kb. Dinucleotides (52.96%) were the most detected SSRs, followed by trinucleotides (34.64%). From the EST-SSR loci, we randomly selected 100 sites for designing primer and used the DNA of 60 samples to verify the polymorphism. The average value of the effective number of alleles (Ne), Shannon’s information index (I), and expective heterozygosity (He) was 1.969, 0.728, and 0.434, respectively. The polymorphism information content (PIC) value was in the range of 0.108 to 0.669, averaging 0.406, which represented a middle polymorphism level. Cluster analysis of S. incisa were also performed based on the obtained EST-SSR data in our work. As shown by structure analysis, 60 individuals could be classified into two groups. Thus, the identification of these novel EST-SSR markers provided valuable sequence information for analyzing the population structure, genetic diversity, and genetic resource assessment of S. incisa and other related species.
Keywords	Medicine ; R ; Science ; Q
Subject code	571
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
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Article ; Online: The unique features of SARS-CoV-2 transmission: Comparison with SARS-CoV, MERS-CoV and 2009 H1N1 pandemic influenza virus.

Wu, Zhonglan / Harrich, David / Li, Zhongyang / Hu, Dongsheng / Li, Dongsheng

Reviews in medical virology

2020 Volume 31, Issue 2, Page(s) e2171

Abstract: From 2002 to 2019, three deadly human coronaviruses (hCoVs), severe acute respiratory syndrome coronavirus (SARS-CoV), Middle Eastern respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) emerged to ... ...

Abstract	From 2002 to 2019, three deadly human coronaviruses (hCoVs), severe acute respiratory syndrome coronavirus (SARS-CoV), Middle Eastern respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) emerged to produce outbreaks of SARS, MERS and coronavirus disease 2019 (Covid-19), respectively. All three hCoVs are members of the Betacoronavirus genus in the subfamily Orthocoronavirinae and share many similarities in virology and epidemiology. However, the pattern and scale of Covid-19 global spread is similar to 2009 pandemic H1N1 influenza (H1N1pdm09), rather than SARS or MERS. Covid-19 exhibits high viral shedding in the upper respiratory tract at an early stage of infection, and has a high proportion of transmission competent individuals that are pre-symptomatic, asymptomatic and mildly symptomatic, characteristics seen in H1N1pdm09 but not in SARS or MERS. These two traits of Covid-19 and H1N1pdm09 result in reduced efficiency in identification of transmission sources by symptomatic screening and play important roles in their ability to spread unchecked to cause pandemics. To overcome these attributes of Covid-19 in community transmission, identifying the transmission source by testing for virus shedding and interrupting chains of transmission by social distancing and public masking are required.
MeSH term(s)	Animals ; COVID-19/epidemiology ; COVID-19/transmission ; COVID-19/virology ; Disease Outbreaks/prevention & control ; Humans ; Influenza A Virus, H1N1 Subtype/pathogenicity ; Influenza, Human/epidemiology ; Influenza, Human/transmission ; Influenza, Human/virology ; Middle East Respiratory Syndrome Coronavirus/pathogenicity ; Pandemics/prevention & control ; SARS Virus/pathogenicity ; SARS-CoV-2/pathogenicity ; Severe Acute Respiratory Syndrome/epidemiology ; Severe Acute Respiratory Syndrome/transmission ; Severe Acute Respiratory Syndrome/virology
Language	English
Publishing date	2020-09-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1086043-5
ISSN	1099-1654 ; 1052-9276
ISSN (online)	1099-1654
ISSN	1052-9276
DOI	10.1002/rmv.2171
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Article ; Online: De novo transcriptomic analysis and identification of EST-SSR markers in Stephanandra incisa.

Zhang, Cuiping / Wu, Zhonglan / Jiang, Xinqiang / Li, Wei / Lu, Yizeng / Wang, Kuiling

Scientific reports

2021 Volume 11, Issue 1, Page(s) 1059

Abstract: Stephanandra incisa is a wild-type shrub with beautiful leaves and white flowers and is commonly used as a garden decoration accessory. However, the limited availability of genomic data of S. incisa has restricted its breeding process. Here, we ... ...

Abstract	Stephanandra incisa is a wild-type shrub with beautiful leaves and white flowers and is commonly used as a garden decoration accessory. However, the limited availability of genomic data of S. incisa has restricted its breeding process. Here, we identified EST-SSR markers using de novo transcriptome sequencing. In this study, a transcriptome database containing 35,251 unigenes, having an average length of 985 bp, was obtained from S. incisa. From these unigene sequences, we identified 5,555 EST-SSRs, with a distribution density of one SSR per 1.60 kb. Dinucleotides (52.96%) were the most detected SSRs, followed by trinucleotides (34.64%). From the EST-SSR loci, we randomly selected 100 sites for designing primer and used the DNA of 60 samples to verify the polymorphism. The average value of the effective number of alleles (Ne), Shannon's information index (I), and expective heterozygosity (He) was 1.969, 0.728, and 0.434, respectively. The polymorphism information content (PIC) value was in the range of 0.108 to 0.669, averaging 0.406, which represented a middle polymorphism level. Cluster analysis of S. incisa were also performed based on the obtained EST-SSR data in our work. As shown by structure analysis, 60 individuals could be classified into two groups. Thus, the identification of these novel EST-SSR markers provided valuable sequence information for analyzing the population structure, genetic diversity, and genetic resource assessment of S. incisa and other related species.
MeSH term(s)	Expressed Sequence Tags ; Genes, Plant/genetics ; Genetic Markers/genetics ; Microsatellite Repeats/genetics ; Polymorphism, Genetic/genetics ; Rosaceae/genetics ; Sequence Analysis, DNA ; Transcriptome
Chemical Substances	Genetic Markers
Language	English
Publishing date	2021-01-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-80329-7
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Article: Cell population data in identifying active tuberculosis and community-acquired pneumonia.

Sun, Tingting / Wu, Bin / Luo, Zhonglan / Wang, Jing / Deng, Shaoli / Huang, Qing

Open medicine (Warsaw, Poland)

2021 Volume 16, Issue 1, Page(s) 1143–1149

Abstract: Objective: Leukocyte morphological parameters known as CPD (cell population data) is detected by hematology analyzer UniCel DxH800 with VCS technology. This study aimed to investigate the diagnostic efficacy of morphological changes in CPD parameters in ...

Abstract	Objective: Leukocyte morphological parameters known as CPD (cell population data) is detected by hematology analyzer UniCel DxH800 with VCS technology. This study aimed to investigate the diagnostic efficacy of morphological changes in CPD parameters in distinguishing active tuberculosis from community-acquired pneumonia. Methods: From October 2018 to February 2019, 88 patients with active tuberculosis, 78 patients with community-acquired pneumonia, and 89 healthy controls were enrolled in this study. CPD was obtained using Unicel DxH800 analyzer for all whole blood samples, one-way ANOVA (non-parametric) and area analysis under ROC curve were performed. Results: The neutrophil mean conductivity (NMC), monocyte mean volume (MMV), monocyte mean conductivity (MMC), lymphocyte percentage (LY%), and monocyte percentage (MO%) were significantly higher in the active tuberculosis group than in the community-acquired pneumonia group. The white blood cell (WBC) count and neutrophil percentage (NE%) were significantly lower in the active tuberculosis group than in the community-acquired pneumonia group. The analysis of the area under the ROC curve proved that WBC count, neutrophil percentage (NE%), lymphocyte percentage (LY%), and monocyte percentage (MO%) did not achieve a higher area under the curve (AUC: 0.63, 0.71, 0.62, and 0.7, respectively). However, the AUC of NMC, MMV, and MMC in the CPD parameters was 0.951, 0.877, 0.98, respectively, and the simultaneous measurement of the three parameters was 0.99. The sensitivity and specificity were 98.5% and 91.1%, respectively. Conclusion: The combined diagnosis of NMC, MMV, and MMC could assist the clinical diagnosis of active tuberculosis and community-acquired pneumonia.
Language	English
Publishing date	2021-08-11
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2829380-0
ISSN	2391-5463
ISSN	2391-5463
DOI	10.1515/med-2021-0322
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Article: Contradictory tendency of As(V) releasing from Fe–As complexes: Influence of organic and inorganic anions

Yang, Zhonglan / Zhang, Nan / Sun, Benhua / Su, Shiming / Wang, Yanan / Zhang, Yang / Wu, Cuixia / Zeng, Xibai

Chemosphere. 2022 Jan., v. 286

2022

Abstract: The strong ability of ferrihydrite and its aged minerals for fixing arsenate is a key factor in remediating arsenate-polluted environments. It is therefore crucial to clarify the stability of Fe–As complexes and the release conditions for As(V). The As(V) ...

Abstract	The strong ability of ferrihydrite and its aged minerals for fixing arsenate is a key factor in remediating arsenate-polluted environments. It is therefore crucial to clarify the stability of Fe–As complexes and the release conditions for As(V). The As(V) release amount was evaluated and compared in the presence of six representative anions, namely, phosphate, silicate, sulfate, inositol hexaphosphate, citrate, and oxalate. It was found that the As(V) release amount changed with the aging time of ferrihydrite and that this tendency generally followed two rules. These are, longer aging time leads to lower As(V) release (Rule 1), and longer aging time leads to higher As(V) release (Rule 2). Whether Rule 1 or Rule 2 dominated As release depended on the number of surface groups, size of competing anions, and contribution of As(V) re-adsorption. Characterization results using X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) provided evidence for the predicted mechanisms of As(V) release under various circumstances. In this work, it was demonstrated that when inorganic anions such as sulfate and silicate are present, ferrihydrite with longer aging time led to decreased As(V) release. When organic anions are present, ferrihydrite with less aging time results in reduced As(V) leaching. For anions such as phosphate, the As(V) release amount in relation to the ferrihydrite aging time depends on the concentration of phosphate ions. Nevertheless, the ligand concentration and As(V) loading rate on ferrihydrite should be simultaneously considered for the rule governing As(V) releasing.
Keywords	Fourier transform infrared spectroscopy ; X-ray diffraction ; X-ray photoelectron spectroscopy ; arsenates ; citrates ; ferrihydrite ; inositols ; ligands ; oxalates ; phosphates ; silicates ; sulfates
Language	English
Dates of publication	2022-01
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2021.131469
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Article ; Online: The unique features of SARS‐CoV‐2 transmission

Wu, Zhonglan / Harrich, David / Li, Zhongyang / Hu, Dongsheng / Li, Dongsheng

Reviews in Medical Virology ; ISSN 1052-9276 1099-1654

Comparison with SARS‐CoV, MERS‐CoV and 2009 H1N1 pandemic influenza virus

2020

Keywords	Virology ; Infectious Diseases ; covid19
Language	English
Publisher	Wiley
Publishing country	us
Document type	Article ; Online
DOI	10.1002/rmv.2171
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Article: Stability of Fe–As composites formed with As(V) and aged ferrihydrite

Yang, Zhonglan / Bai, Lingyu / Su, Shiming / Wang, Yanan / Wu, Cuixia / Zeng, Xibai / Sun, Benhua

Journal of environmental sciences (China). 2021 Feb., v. 100

2021

Abstract: During the aging process, ferrihydrite was transformed into mineral mixtures composed of different proportions of ferrihydrite, goethite, lepidocrocite and hematite. Such a transformation may affect the fixed ability of arsenic. In this study, the ... ...

Abstract	During the aging process, ferrihydrite was transformed into mineral mixtures composed of different proportions of ferrihydrite, goethite, lepidocrocite and hematite. Such a transformation may affect the fixed ability of arsenic. In this study, the stability of Fe-As composites formed with As(V) and the minerals aged for 0, 1, 4, 10 and 30 days of ferrihydrite were systematically examined, and the effects of molar of ratios Fe/As were also clarified using kinetic methods combined with multiple spectroscopic techniques. The results indicated that As(V) was rapidly adsorbed on minerals during the initial polymerization process, which delayed both the ferrihydrite conversion and the hematite formation. When the Fe/As molar ratio was 1.875 and 5.66, the As(V) adsorbed by ferrihydrite began to release after 6 hr and 12 hr, respectively. The corresponding release amounts of As(V) were 0.55 g/L and 0.07 g/L, and the adsorption rates were 92.43% and 97.50% at 60 days, respectively. However, the As(V) adsorbed by the transformation products aged for 30 days of ferrihydrite began to release after adsorbed 30 days. The corresponding release amounts of As(V) were 0.25 g/L and 0.03 g/L, and the adsorption rates were 84.23% and 92.18% after adsorbed 60 days, for the Fe/As=1.875 and 5.66, respectively. Overall, the combination of As(V) with ferrihydrite and aged products transformed from a thermodynamically metastable phase to a dynamically stable state within a certain duration. Moreover, the aging process of ferrihydrite reduced the sorption ability of arsenate by iron (hydr)oxide but enhanced the stability of the Fe-As composites.
Keywords	adsorption ; arsenates ; arsenic ; ferrihydrite ; goethite ; hematite ; iron ; lepidocrocite ; polymerization ; spectroscopy ; thermodynamics ; China
Language	English
Dates of publication	2021-02
Size	p. 43-50.
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	1092300-7
ISSN	1878-7320 ; 1001-0742
ISSN (online)	1878-7320
ISSN	1001-0742
DOI	10.1016/j.jes.2020.07.015
Database	NAL-Catalogue (AGRICOLA)

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Article: Once We Find Grade III Meconium Stained Amniotic Fluid, Must We Act as Early as Possible?

Zhu, Xinxin / Huang, Shiyun / Tang, Yuxuan / Wu, Zhonglan / Sun, Yue / Ren, Huiyan / Lu, Hongmei / Yin, Tingting / Zuo, Qing / Ge, Zhiping / Jiang, Ziyan

International journal of women's health

2023 Volume 15, Page(s) 7–23

Abstract: Background: Grade III meconium stained amniotic fluid (MSAF) is a common obstetric disease, and has the greatest impact on poor maternal and neonatal outcomes.: Question or hypothesis or aim: There is no consensus on treatment, especially on the ... ...

Abstract	Background: Grade III meconium stained amniotic fluid (MSAF) is a common obstetric disease, and has the greatest impact on poor maternal and neonatal outcomes. Question or hypothesis or aim: There is no consensus on treatment, especially on the timing of delivery. Methods: We collected the medical records of 345 women who gave birth with grade III MSAF and analyzed the difference in baseline characteristics and maternal and neonatal outcomes relative to different labor stage, observation times in the first stage of labor, and the presence or absence of abnormal fetal heart rate (FHR) or thick amniotic fluid. Findings: Higher rate of cesarean section was observed when grade III MSAF was found in active labor. Intervention occurred at an observation time of 90-120 min, but there were no significant differences in maternal or neonatal outcomes shown when the observation time was greater than 3 or 4 hours. However, a higher rate of admission to the neonatal intensive care unit was demonstrated in cases with grade III MSAF with abnormal FHR either in the first or second stage of labor or in cases with thick MSAF in the second stage of labor. Discussion: Higher rate of composite adverse neonatal outcomes was found when secondary MSAF (a transition from clear AF to MSAF) was diagnosed >3 h before delivery. Conclusion: In the first stage of labor, an observation time of greater than 4 hours might be possible after grade III MSAF is found if the labor has progressed and is without abnormal FHR.
Language	English
Publishing date	2023-01-05
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2508161-5
ISSN	1179-1411
ISSN	1179-1411
DOI	10.2147/IJWH.S385356
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