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Article ; Online: High-temperature Raman spectra of dipeptide α-L-aspartyl-L-alanine crystal.

Silva, R F / Luz-Lima, C / Freire, P T C / Melo, F E A / Pinheiro, G S

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2023 Volume 297, Page(s) 122746

Abstract: Crystals of dipeptide α-L-aspartyl-L-alanine (α-Asp-Ala), C ...

Abstract	Crystals of dipeptide α-L-aspartyl-L-alanine (α-Asp-Ala), C
Language	English
Publishing date	2023-04-14
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2023.122746
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Article ; Online: Antinociceptive constituents from Saccharum officinarum L. juice

Gomes, Anne C. C. / Soares, Mariana A. / Gomes, Anne K. C. / Silva, Natália L. C. / Miranda, Ana L. P. / Tributino, Jorge L. M. / Luz, Dandara A. / de Amorim, Gisele C. / Simas, Naomi K. / Kuster, Ricardo M.

Natural Product Research. 2021 Dec. 2, v. 35, no. 23 p.5392-5396

2021

Abstract: ... from Saccharum officinarum L. juice and their in vivo antinociceptive potential. After LC-ESI-MS and ESI-MS/MS analysis ... tricin-7-O-(2″- α-L-rhamnopyranosyl)-α-D-galacturonide (1), were identified. MFSC and MFSCf significantly ... highlighting the isolation and the structural elucidation of tricin-7-O-(2″-α-L-rhamnopyranosyl)-α-D ...

Abstract	This work aimed to investigate the main components of methanol fractions (MFSC and MFSCf) from Saccharum officinarum L. juice and their in vivo antinociceptive potential. After LC-ESI-MS and ESI-MS/MS analysis, phenolic compounds, such as dicaffeoylquinic acid, schaftoside, vicenin-2, stilbene glycoside and the major compound tricin-7-O-(2″- α-L-rhamnopyranosyl)-α-D-galacturonide (1), were identified. MFSC and MFSCf significantly inhibited nociceptive responses in classical mice pain models. The isolated flavone, 1, inhibited strongly the neurogenic phase in formalin test without interfering with the inflammatory one. The co-administration of the opioid antagonist, naloxone, significantly reversed the antinociceptive effects on the neurogenic phase of both methanol fractions and 1, demonstrating the involvement of the opioid system on the antinociceptive effect. This work describes for the first time the antinociceptive effect of flavonoids present in sugarcane juice, highlighting the isolation and the structural elucidation of tricin-7-O-(2″-α-L-rhamnopyranosyl)-α-D-galacturonide through ESI-MS/MS, 1D- and 2D-NMR.
Keywords	Saccharum officinarum ; analgesic effect ; antagonists ; flavones ; formalin test ; glycosides ; methanol ; naloxone ; pain ; research ; stilbenes ; sugarcane juice ; Poaceae ; flavonoids ; tricin-7-O-(2″-α-L-rhamnopyranosyl)-α-D-galacturonide ; bidimensional NMR ; antinociception
Language	English
Dates of publication	2021-1202
Size	p. 5392-5396.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2020.1771708
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Diversity and geographical distribution of Leishmania species and the emergence of Leishmania (Leishmania) infantum and L. (Viannia) panamensis in Central-Western Venezuela.

Acta tropica

2023 Volume 242, Page(s) 106901

Abstract: ... sub urban area of the Irribaren municipality. L.(L.) amazonensis appears to be widely dispersed ... L. (L.) infantum as an etiologic agent of cutaneous leishmaniasis in this region. Our findings ... support that Leishmania endemism in central-western Venezuela is caused mainly by L.(L.) amazonensis ...

Abstract	Transmission of cutaneous leishmaniasis in Venezuela reveals diverse and changing epidemiological landscapes, as well as a spectrum of clinical phenotypes presumed to be linked to a variety of Leishmania species. Central-western Venezuela constitutes one of the highest endemic epicenters in the country, and updated molecular epidemiological information is still lacking. Therefore, in this study we aimed to characterize the landscape of circulating Leishmania species across central-western Venezuela through the last two decades, performed comparisons of haplotype and nucleotide diversity, and built a geospatial map of parasite species distribution. A total of 120 clinical samples were collected from patients across the cutaneous disease spectrum, retrieving parasitic DNA, and further characterizing by PCR and sequencing of the HSP70 gene fragment. This data was later collated with further genetic, geospatial and epidemiological analyses. A peculiar pattern of species occurrence including Leishmania (Leishmania) amazonensis (77.63% N=59), Leishmania (Leishmania) infantum (14.47% N=11), Leishmania (Viannia) panamensis (5.26% N=4) and Leishmania (Viannia) braziliensis (2.63% N=2) was revealed, also highlighting a very low genetic diversity amongst all analyzed sequences. Geographical distribution showed that most cases are widely distributed across the greater urban-sub urban area of the Irribaren municipality. L.(L.) amazonensis appears to be widely dispersed throughout Lara state. Statistical analyses failed to reveal significance for any comparisons, leading to conclude a lack of association between the infective Leishmania species and clinical phenotypes. To the best of our knowledge, this is an unprecedented study which addresses comprehensively the geographical distribution of Leishmania species in central-western Venezuela throughout the last two decades, and the first to incriminate L. (L.) infantum as an etiologic agent of cutaneous leishmaniasis in this region. Our findings support that Leishmania endemism in central-western Venezuela is caused mainly by L.(L.) amazonensis. Future studies are needed to unveil additional details on the ecological intricacies and transmission aspects of leishmaniasis (i.e. sampling phlebotomines and mammals) and to adopt adequate public health prevention and control strategies and mitigate disease impact in this endemic region.
MeSH term(s)	Animals ; Leishmania infantum/genetics ; Venezuela/epidemiology ; Leishmaniasis, Cutaneous/epidemiology ; Leishmania braziliensis/genetics ; Leishmania guyanensis/genetics ; Mammals
Language	English
Publishing date	2023-03-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	210415-5
ISSN	1873-6254 ; 0001-706X
ISSN (online)	1873-6254
ISSN	0001-706X
DOI	10.1016/j.actatropica.2023.106901
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Article ; Online: Producción orgánica de Rábano (Raphanus sativus L.) y Lechuga (Lactuca sativa L.).

Yaqueline Antonia Gheno Heredia / Ana María del Pilar Navarro Rodríguez / Norma Berzabel Zilli Ponce / Anylu del Carmen Pérez Monjaras / Luz Anel López Garay

Revista Biológico Agropecuaria Tuxpan, Vol 11, Iss

2023 Volume 1

Abstract: El objetivo del estudio fue conocer el efecto de dos abonos orgánicos en los cultivos de rábano y lechuga en condiciones de cielo abierto. Se evaluaron dos abonos orgánicos (sólido y líquido) en las dos hortalizas, la semilla del rábano se sembró ... ...

Abstract	El objetivo del estudio fue conocer el efecto de dos abonos orgánicos en los cultivos de rábano y lechuga en condiciones de cielo abierto. Se evaluaron dos abonos orgánicos (sólido y líquido) en las dos hortalizas, la semilla del rábano se sembró directamente en las camas y en la lechuga se realizó trasplante. Se usó un diseño completamente al azar evaluando tres tratamientos (testigo, sólido y líquido), cada uno con tres repeticiones. Los datos se analizaron con el programa R Commander versión R x64 4.0.5. No se observaron diferencias estadísticas significativas en ninguna de las variables evaluadas, sin embargo, numéricamente en el rábano, el abono liquido observó mejores promedios en rendimiento fresco y seco de fruto, diámetro longitudinal y altura en la última fecha de muestreo con 4,869 kg ha- 1, 257.09 kg ha-1, 3.38 cm y 28.05 cm, respectivamente. Respecto a la lechuga, el abono líquido también obtuvo los mejores valores en ancho de hoja, rendimiento fresco y seco en hoja y número de hojas con 11.22 cm, 28,098.29 kg ha-1, 15,44.73 kg ha-1 y 25.47 hojas, respectivamente. Los resultados obtenidos en todas las variables evaluadas en las dos hortalizas, son similares a los reportados por otros autores no habiéndose observado diferencias estadísticas significativas debido a que los ciclos de estos cultivos son cortos y la asimilación de nutrientes que se encuentran en los abonos requieren de ciclos prolongados para ser aprovechado por la planta.
Keywords	Rábano ; Lechuga ; Abono orgánico ; Veterinary medicine ; SF600-1100 ; Agriculture ; S
Language	Spanish
Publishing date	2023-07-01T00:00:00Z
Publisher	Editorial Agrícola Tuxpan
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: L&D in the ED

Luz M. Silverio / Esther H. Chen

MedEdPORTAL, Vol

A Game-Based Approach to Learning High-Risk Obstetric Emergencies

2019 Volume 15

Abstract: ... delivery (L&D). To play the game, three to four learners took turns answering questions. Each correct ... while using reference materials. Participants rated the educational value of the L&D board game as 4.81 (out ...

Abstract	Introduction Teaching emergency medicine residents the principles of obstetric emergencies is a challenging task, as these emergencies are uncommon in general practice and include specific maneuvers for difficult and precipitous deliveries. These requirements are not easy to satisfy in a standard lecture and are labor intensive for small groups. This resource is a board game that leverages near-peer teaching, engages student collaboration, and supports friendly competition. Methods The game was designed to be used by emergency medicine residents and emphasized the evaluation and management of acute issues related to labor and delivery (L&D). To play the game, three to four learners took turns answering questions. Each correct answer was awarded points for forward movement on the game board. The first person to reach the end of the game won. A faculty moderator circulated among the players to clarify questions or answers as needed. Results Our residents loved this resource. They were able to teach each other throughout the game while using reference materials. Participants rated the educational value of the L&D board game as 4.81 (out of 5), as compared to 4.37 for prior lectures covering the same topics. The game was rated similarly to delivery procedural skills workshops implemented in prior years (4.62–4.82). Discussion Game-based exercises are low-tech, highly interactive teaching tools enabling students to learn from each other in a fun and engaging way. Although we did not assess knowledge retention, this activity's educational value was rated similarly to delivery procedural skills sessions.
Keywords	Emergency Medicine ; Game ; Obstetrics ; Labor and Delivery ; Precipitous Delivery ; Board Game ; Medicine (General) ; R5-920 ; Education ; L
Subject code	796
Language	English
Publishing date	2019-03-01T00:00:00Z
Publisher	Association of American Medical Colleges
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Assessment of the microbiological quality of natural mineral waters according to the manufacturing time of 20 L returnable packs in Brazil.

Luz, Isabelle da Silva / Vasconcellos, Luiza / Medeiros, Valéria de Mello / Miranda, Catia Aparecida Chaia / Rosas, Carla de Oliveira / Pimenta, Márcia Maria Araújo / Ferreira, Fernando César / Romão, Célia Maria Carvalho Pereira Araújo / Brandão, Marcelo Luiz Lima / Miagostovich, Marize Pereira

FEMS microbiology letters

2020 Volume 367, Issue 15

Abstract: ... in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs ... in 20 L packs with Tm ≥ 2 years presented more microbiological contamination (P ≤ 0.012) than ones ... with a Tm of 0-1 year or a Tm of 1-2 years. These results suggest that the validity period of reusable 20 L ...

Abstract	This study aimed to assess the microbiological quality of natural mineral waters commercialized in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs with different manufacturing times (Tm). With this purpose, 99 water samples from 33 lots (n = 3/batch) of 15 brands, obtained from packs with three intervals of Tm, were analyzed. Total coliforms (16.2%), Pseudomonas aeruginosa (9.9%), sulphite-reducing Clostridium (5.0%) and Escherichia coli (2.0%) were detected but enterococci and norovirus GII not. Regarding brands, 11 (73.3%) presented unsatisfactory results for at least one of the lots analyzed. Pseudomonas aeruginosa analysis revealed six sequence types and strains were susceptible to all antibiotics tested and were able to produce biofilms. Human adenovirus (4) and norovirus GI (9) were also identified in nine samples randomly selected. Natural mineral waters commercialized in 20 L packs with Tm ≥ 2 years presented more microbiological contamination (P ≤ 0.012) than ones with a Tm of 0-1 year or a Tm of 1-2 years. These results suggest that the validity period of reusable 20 L packs should be reduced or that they can no longer be reused.
MeSH term(s)	Bacteria/classification ; Bacteria/isolation & purification ; Brazil ; Mineral Waters/microbiology ; Mineral Waters/virology ; Time Factors ; Viruses/isolation & purification ; Water Microbiology
Chemical Substances	Mineral Waters
Language	English
Publishing date	2020-07-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1093/femsle/fnaa120
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Article ; Online: Seasonal variation in the chemical composition and larvicidal activity against Aedes aegypti L. of essential oils from Brazilian Amazon.

Luz, Tássio Rômulo Silva Araújo / Leite, José Antonio Costa / de Mesquita, Ludmilla Santos Silva / Bezerra, Samara Araújo / Gomes Ribeiro, Edilene Carvalho / Silveira, Daniella Patrícia Brandão / Mesquita, José Wilson Carvalho de / do Amaral, Flávia Maria Mendonça / Coutinho, Denise Fernandes

Experimental parasitology

2022 Volume 243, Page(s) 108405

Abstract: ... transmitted by Aedes aegypti L. are responsible for high rates of morbidity and mortality in the populations ... their larvicidal activity and safety. The species were collected in the rainy and dry periods (Eugenia uniflora L ... Lantana camara L., Ocimum basilicum L., Plectranthus neochilus Schlrt.). The essential oils were extracted ...

Abstract	Arthropod-borne viral diseases are important public health threats in tropical countries. In the Americas, diseases such as Dengue, Zika, Chikungunya, Mayaro, and Yellow fever, which are mainly transmitted by Aedes aegypti L. are responsible for high rates of morbidity and mortality in the populations. The incidence of those viruses has increased in the last few years, due to the decrease of vector control programs, as well as, the resistance of this vector in front of many products that have been used in this proposal. Plant-based products such as essential oils are a promising alternative for Ae. aegypti control. In this context, the present work aims to investigate the seasonality effect on the larvicidal potential and safety of essential oils from 4 endemic plants of the Brazilian Amazon, as well as to verify their larvicidal activity and safety. The species were collected in the rainy and dry periods (Eugenia uniflora L., Lantana camara L., Ocimum basilicum L., Plectranthus neochilus Schlrt.). The essential oils were extracted by hydrodistillation and chemical composition was determined by gas chromatography coupled to mass spectrometry. The larvicidal activity was performed according to the methodology recommended by the World Health Organization. The safety of the oil use was evaluated against two non-target organisms: Artemia salina Leach and Danio rerio Hamilton Buchanan. Essential oils of E. uniflora, L. camara, O. basilicum, P. neochilus, presented as major compounds, in both collection periods, the substances Curzerene, Germacrene D, Methyl chavicol and, Caryophyllene, respectively. However, showed a qualitative and quantitative variation of this composition. All oils showed promising results against Ae. aegypti larvae with LC
MeSH term(s)	Animals ; Humans ; Aedes ; Oils, Volatile/pharmacology ; Oils, Volatile/chemistry ; Mosquito Vectors ; Seasons ; Brazil ; Insecticides/pharmacology ; Insecticides/chemistry ; Gas Chromatography-Mass Spectrometry ; Larva ; Zika Virus ; Zika Virus Infection
Chemical Substances	Oils, Volatile ; Insecticides
Language	English
Publishing date	2022-10-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	391089-1
ISSN	1090-2449 ; 0014-4894
ISSN (online)	1090-2449
ISSN	0014-4894
DOI	10.1016/j.exppara.2022.108405
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Article ; Online: Coconut Flour (Cocos nucifera L.) as a Partial Replacement in Wheat Flour (Triticum aestivum)-Based Tortillas and Its Effect on Dough Rheology and Tortilla Quality.

Islas-Rubio, Alma R / Laborin-Escalante, Francisco / Vásquez-Lara, Francisco / Montoya-Ballesteros, Luz C / Ramos-Clamont Montfort, Gabriela / Calderón de la Barca, Ana M / Heredia-Sandoval, Nina G

Plant foods for human nutrition (Dordrecht, Netherlands)

2023 Volume 78, Issue 2, Page(s) 314–319

Abstract: Wheat-flour (WF) tortillas are among the popular flatbreads used in northern Mexico to prepare 'burritos', which are widely accepted in the USA and other countries but have low nutritional quality. Therefore, to increase the protein and fiber contents, ... ...

Abstract	Wheat-flour (WF) tortillas are among the popular flatbreads used in northern Mexico to prepare 'burritos', which are widely accepted in the USA and other countries but have low nutritional quality. Therefore, to increase the protein and fiber contents, we replaced 10 or 20% of the WF with coconut (Cocos nucifera, variety Alto Saladita) flour (CF) and evaluated the effects on the dough rheology and quality of the composite tortillas. There were differences in the optimum mixing times among the doughs. The protein, fat, and ash contents of the tortillas increased (p < 0.05) with increasing CF content. The carbohydrate content was unchanged (p > 0.05), but the 20% CF tortillas contained more fiber than the control tortilla. Tortilla firmness increased (p < 0.05) and extensibility decreased (p < 0.05) with increasing CF content. The 80:20 blended tortillas showed the highest firmness (7.9 N), whereas the control and 90:10 blended tortillas did not differ (p > 0.05) in firmness. There were no differences (p > 0.05) in extensibility between the composite tortillas. The physicochemical properties of the tortillas indicated that the 20% CF-containing tortilla was a more nutritious option to the wheat flour tortilla due to its higher dietary fiber and protein contents, in addition to the slight reduction seen in extensibility compared to the WF tortilla.
MeSH term(s)	Flour ; Triticum/chemistry ; Cocos ; Bread ; Rheology
Language	English
Publishing date	2023-03-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	188869-9
ISSN	1573-9104 ; 0377-3205
ISSN (online)	1573-9104
ISSN	0377-3205
DOI	10.1007/s11130-023-01049-7
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Article ; Online: A phase 1/2 clinical trial of the nitric oxide synthase inhibitor L-NMMA and taxane for treating chemoresistant triple-negative breast cancer.

Science translational medicine

2021 Volume 13, Issue 624, Page(s) eabj5070

Abstract: ... signaling pathway using the pan-NOS inhibitor NG-monomethyl-l-arginine (L-NMMA) reduced tumor growth and enhanced ... survival in patients with TNBC. Here, we report a first-in-class phase 1/2 trial of L-NMMA combined ... noted in 21% of patients; however, no adverse events were attributed to L-NMMA. Immune cells analyzed ...

Abstract	The inducible nitric oxide signaling (iNOS) pathway is associated with poor prognosis in triple-negative breast cancer (TNBC). Prior studies using in vivo models showed that inhibition of the iNOS signaling pathway using the pan-NOS inhibitor NG-monomethyl-l-arginine (L-NMMA) reduced tumor growth and enhanced survival in patients with TNBC. Here, we report a first-in-class phase 1/2 trial of L-NMMA combined with taxane for treating patients with chemorefractory, locally advanced breast cancer (LABC) or metastatic TNBC. We also examined immune cell correlates of chemotherapy response. 35 patients with metastatic TNBC were recruited: 15 in the phase 1 trial and 24 in the phase 2 trial (including 4 recommended phase 2 dose patients from the phase 1 trial). The overall response rate was 45.8% (11 of 24): 81.8% (9 of 11) for patients with LABC and 15.4% (2 of 13) for patients with metastatic TNBC. Among the patients with LABC, three patients had a pathological complete response at surgery (27.3%). Grade ≥3 toxicity was noted in 21% of patients; however, no adverse events were attributed to L-NMMA. Immune cells analyzed by CyTOF indicated that chemotherapy nonresponders showed greater expression of markers associated with M2 macrophage polarization and increased concentrations of circulating IL-6 and IL-10 cytokines. In contrast, chemotherapy responders showed an increase in CD15
MeSH term(s)	Enzyme Inhibitors/pharmacology ; Humans ; Nitric Oxide/metabolism ; Nitric Oxide Synthase/therapeutic use ; Taxoids/pharmacology ; Taxoids/therapeutic use ; Triple Negative Breast Neoplasms/drug therapy ; Triple Negative Breast Neoplasms/pathology ; omega-N-Methylarginine/pharmacology ; omega-N-Methylarginine/therapeutic use
Chemical Substances	Enzyme Inhibitors ; Taxoids ; omega-N-Methylarginine (27JT06E6GR) ; Nitric Oxide (31C4KY9ESH) ; Nitric Oxide Synthase (EC 1.14.13.39)
Language	English
Publishing date	2021-12-15
Publishing country	United States
Document type	Clinical Trial, Phase I ; Clinical Trial, Phase II ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2518854-9
ISSN	1946-6242 ; 1946-6234
ISSN (online)	1946-6242
ISSN	1946-6234
DOI	10.1126/scitranslmed.abj5070
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Article ; Online: Mango ( Mangifera indica L.) Dehydration as a Women Entrepreneurship Alternative in Vulnerable Communities

Natalia Andrea Salazar-Camacho / Luz Adriana Sanchez-Echeverri / Francisco Javier Fonseca / Víctor Falguera / Daniel Castro-Bocanegra / Nelson Javier Tovar-Perilla

Sustainability, Vol 14, Iss 1548, p

2022 Volume 1548

Abstract: ... inequality. This study evaluated the color and water activity of the mango ( Mangifera indica L.) after ...

Abstract	In Colombia, most commercial drying processes are frequently performed through the burning of wood and fossil fuels with high costs and environmental damage. Nevertheless, solar drying is a technique that could also be used to reduce many of the problems present in traditional drying techniques. Finding low-cost technology to install a solar dryer in a vulnerable community of women is an appropriate alternative; the transfer of knowledge on this technique could lead to greater empowerment of women and prevent gender inequality. This study evaluated the color and water activity of the mango ( Mangifera indica L.) after a drying process in a greenhouse dryer. Furthermore, this study showed the relevance of implementing this technique in a community of women affected by the military conflict in Colombia. Results showed that a solar dryer achieved drying times of around 23–26 h, reaching water activity values below the 0.5 threshold which, according to international standards, has been considered as a food safety value. In addition, the color of the dry mango is slightly affected through this drying technique, providing a more acceptable consumer product. The application of the solar dryer has proven to be an easy-to-use and environmentally friendly method. On the other hand, it is also an opportunity to improve, with a low-cost investment, the living conditions of vulnerable communities.
Keywords	entrepreneurship ; solar drying ; social appropriation ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
Subject code	660
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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