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  1. Book ; Online: Nanobody

    Rothbauer, Ulrich / Chames, Patrick

    2021  

    Keywords Medicine ; Bacillus anthracis ; immunoassay ; single-domain antibody ; genetic fusion ; Beta galactosidase ; P-type ATPase ; nanobody ; llama ; Zinc-transport ; Zinc-transporting P-ATPase ; ZntA ; TNF ; fluorescent ; nanobodies ; sensor ; anti-cytokine therapy ; autoimmune disease ; Western equine encephalitis virus ; MagPlex ; toxin ; antibody ; camelid ; vaccine ; biodefense ; hydrogen exchange-mass spectrometry ; virus ; formatting ; Fc-domain ; half-life ; ischemia ; stroke ; MCAO ; single domain antibodies ; phage display ; intrabody ; intracellular antibody ; GTPase RHO ; BRET ; RAS ; chromobodies ; live-cell imaging ; compound screening ; cellular models ; single-domain antibody fragments ; molecular imaging ; molecular therapy ; nuclear imaging ; targeted fluorescence imaging ; intraoperative imaging ; Nanobody ; Single Domain Antibody ; Cancer ; Immunotherapy ; Imaging ; influenza ; influenza B virus ; hemagglutinin ; single domain antibody ; NanobodyTM ; yeast display ; epitope mapping ; GFP ; C. elegans ; development ; drosophila ; zebrafish ; targeted photodynamic therapy ; hepatocyte growth factor receptor ; HGFR ; c-Met ; Met ; VHH ; photosensitizer ; single-domain antibodies ; neurodegenerative diseases ; brain imaging ; blood-brain barrier ; delivery ; Aids ; HIV ; Llama Antibodies ; bi-specific VHH ; pepscan ; competition studies ; co-crystallisation ; n/a
    Size 1 electronic resource (300 pages)
    Publisher MDPI - Multidisciplinary Digital Publishing Institute
    Publishing place Basel, Switzerland
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021044013
    ISBN 9783036503790 ; 303650379X
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book: Antibody engineering

    Nevoltris, Damien / Chames, Patrick

    methods and protocols

    (Methods in molecular biology ; 1827 ; Springer protocols)

    2018  

    Author's details edited by Damien Nevoltris, Patrick Chames
    Series title Methods in molecular biology ; 1827
    Springer protocols
    Collection
    Language English
    Size xvi, 543 Seiten, Illustrationen
    Edition Third edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT019807462
    ISBN 978-1-4939-8647-7 ; 9781493986484 ; 1-4939-8647-3 ; 1493986481
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1827- verfügbar in Königswinter Königswinter

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  3. Book: Antibody engineering

    Chames, Patrick

    methods and protocols

    (Methods in molecular biology ; 907 ; Springer protocols)

    2012  

    Author's details ed. by Patrick Chames
    Series title Methods in molecular biology ; 907
    Springer protocols
    Collection
    Language English
    Size XVI, 734 S. : Ill., graph. Darst.
    Edition 2. ed.
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    HBZ-ID HT017383167
    ISBN 978-1-61779-973-0 ; 9781617799747 ; 1-61779-973-4 ; 1617799742
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2012 A 3719 order for loan Magazin

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  4. Article ; Online: Specific Targeting of Mesothelin-Expressing Malignant Cells Using Nanobody-Functionalized Magneto-Fluorescent Nanoassemblies.

    Briolay, Tina / Fresquet, Judith / Meyer, Damien / Kerfelec, Brigitte / Chames, Patrick / Ishow, Eléna / Blanquart, Christophe

    International journal of nanomedicine

    2024  Volume 19, Page(s) 633–650

    Abstract: Introduction: Most current anti-cancer therapies are associated with major side effects due to a lack of tumor specificity. Appropriate vectorization of drugs using engineered nanovectors is known to increase local concentration of therapeutic molecules ...

    Abstract Introduction: Most current anti-cancer therapies are associated with major side effects due to a lack of tumor specificity. Appropriate vectorization of drugs using engineered nanovectors is known to increase local concentration of therapeutic molecules in tumors while minimizing their side effects. Mesothelin (MSLN) is a well-known tumor associated antigen overexpressed in many malignancies, in particular in malignant pleural mesothelioma (MPM), and various MSLN-targeting anticancer therapies are currently evaluated in preclinical and clinical assays. In this study, we described, for the first time, the functionalization of fluorescent organic nanoassemblies (NA) with a nanobody (Nb) targeting MSLN for the specific targeting of MSLN expressing MPM cancer cells.
    Methods: Cell lines from different cancer origin expressing or not MSLN were used. An Nb directed against MSLN was coupled to fluorescent NA using click chemistry. A panel of endocytosis inhibitors was used to study targeted NA internalization by cells. Cancer cells were grown in 2D or 3D and under a flow to evaluate the specificity of the targeted NA. Binding and internalization of the targeted NA were studied using flow cytometry, confocal microscopy and transmission electron microscopy.
    Results: We show that the targeted NA specifically bind to MSLN-expressing tumor cells. Moreover, such functionalized NA appear to be internalized more rapidly and in significantly larger proportions compared to naked ones in MSLN+ MPM cells, thereby demonstrating both the functionality and interest of the active targeting strategy. We demonstrated that targeted NA are mainly internalized through a clathrin-independent/dynamin-dependent endocytosis pathway and are directed to lysosomes for degradation. A 3D cell culture model based on MSLN-expressing multicellular tumor spheroids reveals NA penetration in the first superficial layers.
    Conclusion: Altogether, these results open the path to novel anticancer strategies based on MSLN-activated internalization of NA incorporating drugs to promote specific accumulation of active treatments in tumors.
    MeSH term(s) Mesothelin ; Biological Assay ; Cell Line ; Coloring Agents ; Endocytosis
    Chemical Substances Mesothelin (J27WDC343N) ; Coloring Agents
    Language English
    Publishing date 2024-01-20
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2364941-0
    ISSN 1178-2013 ; 1176-9114
    ISSN (online) 1178-2013
    ISSN 1176-9114
    DOI 10.2147/IJN.S435787
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6606: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Special Issue: Nanobody.

    Chames, Patrick / Rothbauer, Ulrich

    Antibodies (Basel, Switzerland)

    2020  Volume 9, Issue 1

    Abstract: Since their first description in 1993 [1], single-domain antibody fragments derived from heavy-chain-only antibodies of camelids have received increasing attention as highly versatile binding molecules in the fields of biotechnology and medicine [ ... ]. ...

    Abstract Since their first description in 1993 [1], single-domain antibody fragments derived from heavy-chain-only antibodies of camelids have received increasing attention as highly versatile binding molecules in the fields of biotechnology and medicine [...].
    Language English
    Publishing date 2020-03-06
    Publishing country Switzerland
    Document type Editorial
    ISSN 2073-4468
    ISSN (online) 2073-4468
    DOI 10.3390/antib9010006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Les anticorps et scaffold bispécifiques, des médicaments innovants en oncologie impliquant le ciblage des cellules immunitaires.

    Chames, Patrick / Wurch, Thierry

    Medecine sciences : M/S

    2020  Volume 35, Issue 12, Page(s) 1072–1082

    Abstract: Over the past ten years, an increased knowledge of tumor biology and immunology allowed the design and development of novel therapeutic antibody and protein scaffold formats, where bispecific antibodies (Abs) play a major role. The latter molecules can ( ... ...

    Title translation Bispecific antibodies, novel therapeutic candidates harnessing the immune system.
    Abstract Over the past ten years, an increased knowledge of tumor biology and immunology allowed the design and development of novel therapeutic antibody and protein scaffold formats, where bispecific antibodies (Abs) play a major role. The latter molecules can (1) bring novel pharmacological properties through the co-engagement of two targets, (2) increase the safety profile as compared to a combination of two antibodies thanks to a targeted relocation to the tumor and (3) reduce development and manufacturing costs associated with single drug product. This review analyzes the different bispecific antibodies and scaffolds described in the field of immuno-oncology, their structure and major pharmacological and physico-chemical properties.
    MeSH term(s) Antibodies, Bispecific/therapeutic use ; Antigens, Neoplasm ; Humans ; Immune System/drug effects ; Immune System/physiology ; Immunotherapy/methods ; Immunotherapy/trends ; Molecular Targeted Therapy/methods ; Molecular Targeted Therapy/trends ; Neoplasms/immunology ; Neoplasms/pathology ; Neoplasms/therapy ; Protein Engineering/methods ; Protein Engineering/trends ; Therapies, Investigational/methods ; Therapies, Investigational/trends
    Chemical Substances Antibodies, Bispecific ; Antigens, Neoplasm
    Language French
    Publishing date 2020-01-06
    Publishing country France
    Document type Journal Article ; Review
    ZDB-ID 632733-3
    ISSN 1958-5381 ; 0767-0974
    ISSN (online) 1958-5381
    ISSN 0767-0974
    DOI 10.1051/medsci/2019242
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2872: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Epitope Identification of an mGlu5 Receptor Nanobody Using Physics-Based Molecular Modeling and Deep Learning Techniques.

    Eshak, Floriane / Pion, Léo / Scholler, Pauline / Nevoltris, Damien / Chames, Patrick / Rondard, Philippe / Pin, Jean-Philippe / Acher, Francine C / Goupil-Lamy, Anne

    Journal of chemical information and modeling

    2024  

    Abstract: The world has witnessed a revolution in therapeutics with the development of biological medicines such as antibodies and antibody fragments, notably nanobodies. These nanobodies possess unique characteristics including high specificity and modulatory ... ...

    Abstract The world has witnessed a revolution in therapeutics with the development of biological medicines such as antibodies and antibody fragments, notably nanobodies. These nanobodies possess unique characteristics including high specificity and modulatory activity, making them promising candidates for therapeutic applications. Identifying their binding mode is essential for their development. Experimental structural techniques are effective to get such information, but they are expensive and time-consuming. Here, we propose a computational approach, aiming to identify the epitope of a nanobody that acts as an agonist and a positive allosteric modulator at the rat metabotropic glutamate receptor 5. We employed multiple structure modeling tools, including various artificial intelligence algorithms for epitope mapping. The computationally identified epitope was experimentally validated, confirming the success of our approach. Additional dynamics studies provided further insights on the modulatory activity of the nanobody. The employed methodologies and approaches initiate a discussion on the efficacy of diverse techniques for epitope mapping and later nanobody engineering.
    Language English
    Publishing date 2024-02-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 190019-5
    ISSN 1549-960X ; 0095-2338
    ISSN (online) 1549-960X
    ISSN 0095-2338
    DOI 10.1021/acs.jcim.3c01620
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1230: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Nanobody Engineering: Toward Next Generation Immunotherapies and Immunoimaging of Cancer.

    Chanier, Timothée / Chames, Patrick

    Antibodies (Basel, Switzerland)

    2019  Volume 8, Issue 1

    Abstract: In the last decade, cancer immunotherapies have produced impressive therapeutic results. However, the potency of immunotherapy is tightly linked to immune cell infiltration within the tumor and varies from patient to patient. Thus, it is becoming ... ...

    Abstract In the last decade, cancer immunotherapies have produced impressive therapeutic results. However, the potency of immunotherapy is tightly linked to immune cell infiltration within the tumor and varies from patient to patient. Thus, it is becoming increasingly important to monitor and modulate the tumor immune infiltrate for an efficient diagnosis and therapy. Various bispecific approaches are being developed to favor immune cell infiltration through specific tumor targeting. The discovery of antibodies devoid of light chains in camelids has spurred the development of single domain antibodies (also called VHH or nanobody), allowing for an increased diversity of multispecific and/or multivalent formats of relatively small sizes endowed with high tissue penetration. The small size of nanobodies is also an asset leading to high contrasts for non-invasive imaging. The approval of the first therapeutic nanobody directed against the von Willebrand factor for the treatment of acquired thrombotic thrombocypenic purpura (Caplacizumab, Ablynx), is expected to bolster the rise of these innovative molecules. In this review, we discuss the latest advances in the development of nanobodies and nanobody-derived molecules for use in cancer immunotherapy and immunoimaging.
    Language English
    Publishing date 2019-01-21
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2661514-9
    ISSN 2073-4468 ; 2073-4468
    ISSN (online) 2073-4468
    ISSN 2073-4468
    DOI 10.3390/antib8010013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Phage Display and Selections on Purified Antigens.

    Colazet, Magali / Chames, Patrick

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1827, Page(s) 165–178

    Abstract: The isolation of antibody fragments targeting proteins implicated in cancers and other diseases remains a crucial issue on targeted therapy or diagnostic tool development. In many case, the protein of interest, or a relevant portion of this protein such ... ...

    Abstract The isolation of antibody fragments targeting proteins implicated in cancers and other diseases remains a crucial issue on targeted therapy or diagnostic tool development. In many case, the protein of interest, or a relevant portion of this protein such as its extracellular domain, is available as purified protein. In such cases, phage display on purified antigen is an easy and fast way to select antibody fragment able to efficiently bind this antigen. However the output of phage selection can vary significantly depending on the way to immobilize the purified antigen during selection. The following protocols describe the selection of phage antibody on purified antigen adsorbed on plastic, i.e., panning, or a selection in solution, using a biotinylated antigen as well as the corresponding screening produces, and give hints on the advantage and drawbacks of each approach.
    MeSH term(s) Antibodies/metabolism ; Antigens/isolation & purification ; Bacteriophages/metabolism ; Biotinylation ; Cell Surface Display Techniques/methods ; Chemical Precipitation ; Enzyme-Linked Immunosorbent Assay ; Peptide Library ; Solubility
    Chemical Substances Antibodies ; Antigens ; Peptide Library
    Language English
    Publishing date 2018-09-09
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-8648-4_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Rapid nanobody-based imaging of mesothelin expressing malignancies compatible with blocking therapeutic antibodies.

    Benloucif, Abdennour / Meyer, Damien / Balasse, Laure / Goubard, Armelle / Danner, Lucile / Bouhlel, Ahlem / Castellano, Rémy / Guillet, Benjamin / Chames, Patrick / Kerfelec, Brigitte

    Frontiers in immunology

    2023  Volume 14, Page(s) 1200652

    Abstract: Introduction: Mesothelin (MSLN) is overexpressed in a wide variety of cancers with few therapeutic options and has recently emerged as an attractive target for cancer therapy, with a large number of approaches currently under preclinical and clinical ... ...

    Abstract Introduction: Mesothelin (MSLN) is overexpressed in a wide variety of cancers with few therapeutic options and has recently emerged as an attractive target for cancer therapy, with a large number of approaches currently under preclinical and clinical investigation. In this respect, developing mesothelin specific tracers as molecular companion tools for predicting patient eligibility, monitoring then response to mesothelin-targeting therapies, and tracking the evolution of the disease or for real-time visualisation of tumours during surgery is of growing importance.
    Methods: We generated by phage display a nanobody (Nb S1) and used enzymatic approaches were used to site-directed conjugate Nb S1 with either ATTO 647N fluorochrome or NODAGA chelator for fluorescence and positron emission tomography imaging (PET) respectively.
    Results: We demonstrated that Nb S1 displays a high apparent affinity and specificity for human mesothelin and demonstrated that the binding, although located in the membrane distal domain of mesothelin, is not impeded by the presence of MUC16, the only known ligand of mesothelin, nor by the therapeutic antibody amatuximab.
    Conclusion: We demonstrated for the first time the use of an anti-MSLN nanobody as PET radiotracer for same day imaging of MSLN
    MeSH term(s) Humans ; Mesothelin ; Tissue Distribution ; Positron-Emission Tomography ; Neoplasms/diagnostic imaging ; Neoplasms/therapy ; Antibodies, Blocking
    Chemical Substances 1-(1,3-carboxypropyl)-4,7-carboxymethyl-1,4,7-triazacyclononane ; Mesothelin (J27WDC343N) ; Antibodies, Blocking
    Language English
    Publishing date 2023-06-14
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1200652
    Database MEDical Literature Analysis and Retrieval System OnLINE

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