LIVIVO - Search results -

Search results

Result 1 - 10 of total 2855

Search options

Article ; Online: Gestion des sols et des substrats pour la nature et l’agriculture urbaines à Strasbourg

Adrien Baysse-Lainé / Laure Cormier / Kevin Gaulier

Projets de Paysage, Vol

vers un renouvellement des représentations et des pratiques des circulations de matière ?

2022 Volume 27

Abstract: The development of natural and agricultural areas in cities which is accompanied by the circulation of topsoil along with other substrates or amendments is rarely studied. In fact, the attention of landscape architects and urban planners does not ... ...

Abstract	The development of natural and agricultural areas in cities which is accompanied by the circulation of topsoil along with other substrates or amendments is rarely studied. In fact, the attention of landscape architects and urban planners does not directly focus on the soils which are often considered as secondary components of plant-substrate combinations reconstituted ex nihilo. Based on an exploratory qualitative survey in Strasbourg, the article investigates the modes of soil management in urban nature and agricultural projects between which tensions exist due to the differing conceptions of soils as (situated) ecosystems and as materials (to be employed for producing spaces and landscapes defined ex ante). Soil inputs from outside the sites in development are part of a linear economy, while the production of soil amendments (primarily compost) from urban waste illustrates the trend towards a circular economy. As a result, the in situ management of inherited soils remains limited to community gardens and a joint development zone (in French zone d’aménagement concerté or ZAC) due to economic reasons and emerging political constraints. Although not exhaustive, the cross-sectional analysis on the scale of a city presented in the article sheds light on the systemic and multi-scalar nature of the circulation of materials, as well as of the trends affecting the balances between the typologies of circuits and materials.
Keywords	landscape enterprises ; composting ; circular economy ; Eurométropole ; topsoil ; Social Sciences ; H
Subject code	710
Language	French
Publishing date	2022-12-01T00:00:00Z
Publisher	Agrocampus Angers, Ecole nationale supérieure du paysage, ENP Blois, ENSAP Bordeaux, ENSAP Lille
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Marie-Claude Maurel, 2021, Terre et propriété à l’est de l’Europe depuis 1990. Faisceau de droits, relations de pouvoir, Besançon, Presses universitaires de Franche-Comté, coll. « Les cahiers de la MSHE Ledoux », 246 pages.

Adrien Baysse-Lainé

Développement Durable et Territoires, Vol

2021 Volume 12

Keywords	Environmental sciences ; GE1-350 ; Social Sciences ; H
Language	French
Publishing date	2021-11-01T00:00:00Z
Publisher	Réseau Développement Durable et Territoires Fragiles
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: The control exerted by ABA on lignan biosynthesis in flax (Linum usitatissimum L.) is modulated by a Ca

Markulin, Lucija / Drouet, Samantha / Corbin, Cyrielle / Decourtil, Cédric / Garros, Laurine / Renouard, Sullivan / Lopez, Tatiana / Mongelard, Gaëlle / Gutierrez, Laurent / Auguin, Daniel / Lainé, Eric / Hano, Christophe

Journal of plant physiology

2019 Volume 236, Page(s) 74–87

Abstract: ... L.). Abscisic acid (ABA) plays a key role in the regulation of LuPLR1 gene expression and lignan ...

Abstract	The LuPLR1 gene encodes a pinoresinol lariciresinol reductase responsible for the biosynthesis of (+)-secoisolariciresinol, a cancer chemopreventive lignan, highly accumulated in the seedcoat of flax (Linum usitatissimum L.). Abscisic acid (ABA) plays a key role in the regulation of LuPLR1 gene expression and lignan accumulation in both seeds and cell suspensions, which require two cis-acting elements (ABRE and MYB2) for this regulation. Ca
MeSH term(s)	Abscisic Acid/metabolism ; Abscisic Acid/physiology ; Butylene Glycols/metabolism ; Calcium/metabolism ; Calmodulin/metabolism ; Chromatography, High Pressure Liquid ; Flax/metabolism ; Gene Expression Regulation, Plant ; Glucuronidase/metabolism ; Lignans/biosynthesis ; Lignans/metabolism ; Plant Growth Regulators/metabolism ; Plant Growth Regulators/physiology ; Plant Proteins/metabolism ; Protein Kinase C/metabolism ; Real-Time Polymerase Chain Reaction ; Signal Transduction/physiology ; Transcriptome
Chemical Substances	Butylene Glycols ; Calmodulin ; Lignans ; Plant Growth Regulators ; Plant Proteins ; Abscisic Acid (72S9A8J5GW) ; Protein Kinase C (EC 2.7.11.13) ; Glucuronidase (EC 3.2.1.31) ; secoisolariciresinol (M8QRJ7JEJH) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2019-03-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	283647-6
ISSN	1618-1328 ; 0176-1617
ISSN (online)	1618-1328
ISSN	0176-1617
DOI	10.1016/j.jplph.2019.03.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 46/302: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Assessing the Effect of Silicon Supply on Root Sulfur Uptake in S-Fed and S-Deprived Brassica napus L

Laîné, Philippe / Coquerel, Raphaël / Arkoun, Mustapha / Trouverie, Jacques / Etienne, Philippe

Plants. 2022 June 18, v. 11, no. 12

2022

Abstract: Silicon (Si) is known to alleviate many nutritional stresses. However, in Brassica napus, which is a highly S-demanding species, the Si effect on S deficiency remains undocumented. The aim of this study was to assess whether Si alleviates the negative ... ...

Abstract	Silicon (Si) is known to alleviate many nutritional stresses. However, in Brassica napus, which is a highly S-demanding species, the Si effect on S deficiency remains undocumented. The aim of this study was to assess whether Si alleviates the negative effects of S deficiency on Brassica napus and modulates root sulfate uptake capacity and S accumulation. For this, Brassica napus plants were cultivated with or without S and supplied or not supplied with Si. The effects of Si on S content, growth, expression of sulfate transporter genes (BnaSultr1.1; BnaSultr1.2) and sulfate transporters activity in roots were monitored. Si supply did not mitigate growth or S status alterations due to S deprivation but moderated the expression of BnaSultr1.1 in S-deprived plants without affecting the activity of root sulfate transporters. The effects of Si on the amount of S taken-up and on S transporter gene expression were also evaluated after 72 h of S resupply. In S-deprived plants, S re-feeding led to a strong decrease in the expression of both S transporter genes as expected, except in Si-treated plants where BnaSultr1.1 expression was maintained over time. This result is discussed in relation to the similar amount of S accumulated regardless of the Si treatment.
Keywords	Brassica napus ; gene expression ; refeeding ; silicon ; sulfates ; sulfur
Language	English
Dates of publication	2022-0618
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11121606
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Nitrogen transfer from Lupinus albus L., Trifolium incarnatum L. and Vicia sativa L. contribute differently to rapeseed (Brassica napus L.) nitrogen nutrition.

Génard, Thaïs / Etienne, Philippe / Laîné, Philippe / Yvin, Jean-Claude / Diquélou, Sylvain

Heliyon

2016 Volume 2, Issue 9, Page(s) e00150

Abstract: ... The present study was carried out to quantify N transfer from legumes (Lupinus albus L., Trifolium incarnatum ... L. or Vicia sativa L.) to rapeseed (Brassica napus L.) using the split-root (15)N-labelling method ...

Abstract	Nitrogen (N) transfer is well documented in legume-cereal intercropping but this is less often reported for legume-Brassica intercrops even though Brassica crops require higher levels of N fertilizers. The present study was carried out to quantify N transfer from legumes (Lupinus albus L., Trifolium incarnatum L. or Vicia sativa L.) to rapeseed (Brassica napus L.) using the split-root (15)N-labelling method. After three months we observed that legumes did not alter the growth of rapeseed. Vetch showed the lowest growth and demonstrated low (15)N shoot to root translocation and no significant N transfer to rapeseed. In contrast, significant (15)N enrichment was found in lupine and clover and (15)N was transferred to the associated rapeseed plants (around 6 and 4 mg N plant(-1), respectively), which contributed 2 to 3% of the rapeseed total N. Additionally, the data revealed that N2 fixation dominated the N nutrition in lupine despite the high N level provided in the donor compartment, suggesting a greater niche segregation between companion plants. Based on the results of this study we suggest that intercropping can be a relevant contributor to rapeseed N nutrition. Among the three legumes tested, clover and lupine seemed to be the best intercropping candidates.
Language	English
Publishing date	2016-09-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2016.e00150
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Prevalence of contamination by intestinal parasites in vegetables (Lactuca sativa L. and Coriandrum sativum L.) sold in markets in Belém, northern Brazil.

Rodrigues, Alessandro Cardoso / da Silva, Melanie Dayane Castro / Pereira, Regina Ângela Seixas / Pinto, Laine Celestino

Journal of the science of food and agriculture

2020 Volume 100, Issue 7, Page(s) 2859–2865

Abstract: ... Lactuca sativa L.) and coriander (Coriandrum sativum L.) destined for human consumption. This study determined ...

Abstract	Background: Previous studies have recorded a high prevalence of intestinal parasites in lettuce (Lactuca sativa L.) and coriander (Coriandrum sativum L.) destined for human consumption. This study determined the prevalence of contamination by intestinal parasites in these two plants sold in two street markets and two supermarkets in the city of Belém, northern Brazil. Results: A total of 200 plant samples were analyzed (100 of each species). The samples were collected randomly between August and October 2018, examined by the spontaneous sedimentation method with two washes, and stored for 24 h. The analysis found that 89% (89/100) of the lettuce samples and 86% (86/100) of the coriander samples were contaminated. Polyparasitism was more frequent in lettuce, but monoparasitism predominated in the coriander. A total of 226 intestinal parasites were found in the lettuce, with a predominance of non-pathogenic parasites in the supermarket samples and more pathogenic parasites in the samples from street markets. In the coriander samples, 172 intestinal parasites were identified, with a predominance of pathogenic parasites in samples from both types of market. In the case of the protozoans, the most prevalent species was Endolimax nana, followed by Blastocystis hominis, in both vegetables. In the helminths, hookworms predominated in the lettuce, and Trichuris trichiura in the coriander. Conclusions: These results highlight the need for the monitoring of parasite contamination in vegetables destined for human consumption. Public health initiatives should include educational campaigns on the importance of disinfecting vegetables prior to consumption. © 2020 Society of Chemical Industry.
MeSH term(s)	Amoebida/classification ; Amoebida/isolation & purification ; Animals ; Brazil ; Coriandrum/parasitology ; Cross-Sectional Studies ; Food Contamination/analysis ; Helminths/classification ; Helminths/isolation & purification ; Lactuca/parasitology ; Parasites/classification ; Parasites/isolation & purification ; Prevalence
Language	English
Publishing date	2020-03-03
Publishing country	England
Document type	Journal Article
ZDB-ID	184116-6
ISSN	1097-0010 ; 0022-5142
ISSN (online)	1097-0010
ISSN	0022-5142
DOI	10.1002/jsfa.10265
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 808: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Silicon supply affects the root transcriptome of Brassica napus L.

Haddad, Cylia / Trouverie, Jacques / Arkoun, Mustapha / Yvin, Jean-Claude / Caïus, José / Brunaud, Véronique / Laîné, Philippe / Etienne, Philippe

Planta

2019 Volume 249, Issue 5, Page(s) 1645–1651

Abstract: Main conclusion: Modulation of gene expression in roots of Brassica napus by silicon (Si) supply could allow plants to cope with future stresses. The origin of the beneficial effects of silicon (Si) in plants, especially when they are subject to stress, ...

Abstract	Main conclusion: Modulation of gene expression in roots of Brassica napus by silicon (Si) supply could allow plants to cope with future stresses. The origin of the beneficial effects of silicon (Si) in plants, especially when they are subject to stress, remains poorly understood. Some authors have shown that Si alleviates plant stress and consider that this is mainly due to a mechanical effect on the cell wall. In addition, the other studies have shown that Si can also affect gene expression and modulate a number of metabolic pathways, especially in plants cultivated under stress conditions. Previously, Haddad et al. (Front Plant Sci 9:5-16, 2018) showed that a pretreatment of Brassica napus plants with Si (1.7 mM) for 1 week alleviated the stress induced by N privation. These results suggest that this improved resistance in Si-treated plants might be due to the establishment of defense mechanisms prior to exposure to the N stress. The aim of the current work was to test this assumption in Brassica napus roots (where Si is mainly stored) using a transcriptomic approach via the RNA sequencing. Our results indicated that the Si supply leads to a modulation of the expression of genes in Brassica napus roots. Functional categorization of the differentially expressed genes demonstrated that numerous genes are involved in different metabolic pathways and especially in cell wall synthesis, phytohormone metabolism, and stress responses. All these results show that Si modifies the root metabolism of B. napus, which could allow a better adaptation to future stresses.
MeSH term(s)	Brassica napus/drug effects ; Brassica napus/genetics ; Brassica napus/metabolism ; Gene Expression Regulation, Plant/drug effects ; Gene Expression Regulation, Plant/genetics ; Plant Roots/drug effects ; Plant Roots/genetics ; Silicon/pharmacology ; Transcriptome/drug effects ; Transcriptome/genetics
Chemical Substances	Silicon (Z4152N8IUI)
Language	English
Publishing date	2019-02-28
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/s00425-019-03120-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: A genome-wide analysis of the flax (Linum usitatissimum L.) dirigent protein family: from gene identification and evolution to differential regulation.

Corbin, Cyrielle / Drouet, Samantha / Markulin, Lucija / Auguin, Daniel / Lainé, Éric / Davin, Laurence B / Cort, John R / Lewis, Norman G / Hano, Christophe

Plant molecular biology

2018 Volume 97, Issue 1-2, Page(s) 73–101

Abstract: Key message: Identification of DIR encoding genes in flax genome. Analysis of phylogeny, gene/protein structures and evolution. Identification of new conserved motifs linked to biochemical functions. Investigation of spatio-temporal gene expression and ... ...

Abstract	Key message: Identification of DIR encoding genes in flax genome. Analysis of phylogeny, gene/protein structures and evolution. Identification of new conserved motifs linked to biochemical functions. Investigation of spatio-temporal gene expression and response to stress. Dirigent proteins (DIRs) were discovered during 8-8' lignan biosynthesis studies, through identification of stereoselective coupling to afford either (+)- or (-)-pinoresinols from E-coniferyl alcohol. DIRs are also involved or potentially involved in terpenoid, allyl/propenyl phenol lignan, pterocarpan and lignin biosynthesis. DIRs have very large multigene families in different vascular plants including flax, with most still of unknown function. DIR studies typically focus on a small subset of genes and identification of biochemical/physiological functions. Herein, a genome-wide analysis and characterization of the predicted flax DIR 44-membered multigene family was performed, this species being a rich natural grain source of 8-8' linked secoisolariciresinol-derived lignan oligomers. All predicted DIR sequences, including their promoters, were analyzed together with their public gene expression datasets. Expression patterns of selected DIRs were examined using qPCR, as well as through clustering analysis of DIR gene expression. These analyses further implicated roles for specific DIRs in (-)-pinoresinol formation in seed-coats, as well as (+)-pinoresinol in vegetative organs and/or specific responses to stress. Phylogeny and gene expression analysis segregated flax DIRs into six distinct clusters with new cluster-specific motifs identified. We propose that these findings can serve as a foundation to further systematically determine functions of DIRs, i.e. other than those already known in lignan biosynthesis in flax and other species. Given the differential expression profiles and inducibility of the flax DIR family, we provisionally propose that some DIR genes of unknown function could be involved in different aspects of secondary cell wall biosynthesis and plant defense.
MeSH term(s)	Amino Acid Motifs ; Butylene Glycols/metabolism ; Cell Wall/genetics ; Cell Wall/metabolism ; Evolution, Molecular ; Flax/classification ; Flax/genetics ; Gene Expression Regulation, Plant ; Lignans/metabolism ; Multigene Family ; Phylogeny ; Plant Proteins/chemistry ; Plant Proteins/genetics ; Real-Time Polymerase Chain Reaction
Chemical Substances	Butylene Glycols ; Lignans ; Plant Proteins ; secoisolariciresinol (M8QRJ7JEJH)
Language	English
Publishing date	2018-04-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	778032-1
ISSN	1573-5028 ; 0167-4412
ISSN (online)	1573-5028
ISSN	0167-4412
DOI	10.1007/s11103-018-0725-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3458: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Characterization of LuWRKY36, a flax transcription factor promoting secoisolariciresinol biosynthesis in response to Fusarium oxysporum elicitors in Linum usitatissimum L. hairy roots.

Markulin, Lucija / Corbin, Cyrielle / Renouard, Sullivan / Drouet, Samantha / Durpoix, Charlène / Mathieu, Charlotte / Lopez, Tatiana / Auguin, Daniel / Hano, Christophe / Lainé, Éric

Planta

2019 Volume 250, Issue 1, Page(s) 347–366

Abstract: Main conclusion: The involvement of a WRKY transcription factor in the regulation of lignan biosynthesis in flax using a hairy root system is described. Secoisolariciresinol is the main flax lignan synthesized by action of LuPLR1 (pinoresinol- ... ...

Abstract	Main conclusion: The involvement of a WRKY transcription factor in the regulation of lignan biosynthesis in flax using a hairy root system is described. Secoisolariciresinol is the main flax lignan synthesized by action of LuPLR1 (pinoresinol-lariciresinol reductase 1). LuPLR1 gene promoter deletion experiments have revealed a promoter region containing W boxes potentially responsible for the response to Fusarium oxysporum. W boxes are bound by WRKY transcription factors that play a role in the response to stress. A candidate WRKY transcription factor, LuWRKY36, was isolated from both abscisic acid and Fusarium elicitor-treated flax cell cDNA libraries. This transcription factors contains two WRKY DNA-binding domains and is a homolog of AtWRKY33. Different approaches confirmed LuWRKY36 binding to a W box located in the LuPLR1 promoter occurring through a unique direct interaction mediated by its N-terminal WRKY domain. Our results propose that the positive regulator action of LuWRKY36 on the LuPLR1 gene regulation and lignan biosynthesis in response to biotic stress is positively mediated by abscisic acid and inhibited by ethylene. Additionally, we demonstrate a differential Fusarium elicitor response in susceptible and resistant flax cultivars, seen as a faster and stronger LuPLR1 gene expression response accompanied with higher secoisolariciresinol accumulation in HR of the resistant cultivar.
MeSH term(s)	Abscisic Acid/pharmacology ; Ethylenes/pharmacology ; Flax/genetics ; Flax/metabolism ; Flax/microbiology ; Fusarium/physiology ; Gene Library ; Lignans/biosynthesis ; Models, Biological ; Oxidoreductases/genetics ; Oxidoreductases/metabolism ; Plant Diseases/microbiology ; Plant Growth Regulators/pharmacology ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Roots/genetics ; Plant Roots/metabolism ; Plant Roots/microbiology ; Promoter Regions, Genetic/genetics ; Stress, Physiological ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	Ethylenes ; Lignans ; Plant Growth Regulators ; Plant Proteins ; Transcription Factors ; Abscisic Acid (72S9A8J5GW) ; ethylene (91GW059KN7) ; Oxidoreductases (EC 1.-)
Language	English
Publishing date	2019-04-29
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/s00425-019-03172-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Antimetabolic cooperativity with the clinically approved l-asparaginase and tyrosine kinase inhibitors to eradicate CML stem cells.

Trinh, Anne / Khamari, Raeeka / Fovez, Quentin / Mahon, François-Xavier / Turcq, Béatrice / Bouscary, Didier / Maboudou, Patrice / Joncquel, Marie / Coiteux, Valérie / Germain, Nicolas / Laine, William / Dekiouk, Salim / Jean-Pierre, Sandrine / Maguer-Satta, Veronique / Ghesquiere, Bart / Idziorek, Thierry / Quesnel, Bruno / Kluza, Jerome / Marchetti, Philippe

Molecular metabolism

2021 Volume 55, Page(s) 101410

Abstract: ... as well as primary CD34+ stem-like cells from CML patients exposed to TKI and L-Asparaginases. Colony ... CML cell survival. Glutamine metabolism was inhibited by L-asparaginases such as Kidrolase or Erwinase ...

Abstract	Objective: Long-term treatment with tyrosine kinase inhibitors (TKI) represents an effective cure for chronic myeloid leukemia (CML) patients and discontinuation of TKI therapy is now proposed to patient with deep molecular responses. However, evidence demonstrating that TKI are unable to fully eradicate dormant leukemic stem cells (LSC) indicate that new therapeutic strategies are needed to control LSC and to prevent relapse. In this study we investigated the metabolic pathways responsible for CML surviving to imatinib exposure and its potential therapeutic utility to improve the efficacy of TKI against stem-like CML cells. Methods: Using complementary cell-based techniques, metabolism was characterized in a large panel of BCR-ABL+ cell lines as well as primary CD34+ stem-like cells from CML patients exposed to TKI and L-Asparaginases. Colony forming cell (CFC) assay and flow cytometry were used to identify CML progenitor and stem like-cells. Preclinical models of leukemia dormancy were used to test the effect of treatments. Results: Although TKI suppressed glycolysis, compensatory glutamine-dependent mitochondrial oxidation supported ATP synthesis and CML cell survival. Glutamine metabolism was inhibited by L-asparaginases such as Kidrolase or Erwinase without inducing predominant CML cell death. However, clinically relevant concentrations of TKI render CML cells susceptible to Kidrolase. The combination of TKI with Lasparaginase reactivates the intinsic apoptotic pathway leading to efficient CML cell death. Conclusion: Targeting glutamine metabolism with the FDA-approved drug, Kidrolase in combination with TKI that suppress glycolysis represents an effective and widely applicable therapeutic strategy for eradicating stem-like CML cells.
MeSH term(s)	Animals ; Apoptosis/drug effects ; Asparaginase/metabolism ; Asparaginase/pharmacology ; Asparagine/antagonists & inhibitors ; Asparagine/metabolism ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Drug Resistance, Neoplasm/drug effects ; Humans ; Imatinib Mesylate/metabolism ; Imatinib Mesylate/pharmacology ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism ; Mice ; Neoplastic Stem Cells/drug effects ; Neoplastic Stem Cells/metabolism ; Protein Kinase Inhibitors/pharmacology ; Protein-Tyrosine Kinases/antagonists & inhibitors ; Protein-Tyrosine Kinases/metabolism
Chemical Substances	Protein Kinase Inhibitors ; Asparagine (7006-34-0) ; Imatinib Mesylate (8A1O1M485B) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; Asparaginase (EC 3.5.1.1)
Language	English
Publishing date	2021-12-01
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2708735-9
ISSN	2212-8778 ; 2212-8778
ISSN (online)	2212-8778
ISSN	2212-8778
DOI	10.1016/j.molmet.2021.101410
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito