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Article ; Online: Secreted phosphoprotein 1 regulates natural compound 3',4',5,7-tetrahydroxyflavone to inhibit mast cell-mediated allergic inflammation.

Hu, Shiling / Wang, Jue / Bai, Haoyun / Feng, Chaohua / Zhou, Zhenqi / Xue, Zhuoyin / Zhang, Wen / Zhang, Yongjing / Wang, Nan / He, Langchong

Immunopharmacology and immunotoxicology

2023 Volume 45, Issue 6, Page(s) 672–681

Abstract: Background: Mast cells (MCs) are important effector cells in anaphylaxis and anaphylactic disease. 3',4',5,7-tetrahydroxyflavone (THF) presents in many medicinal plants and exerts a variety of pharmacological effects. In this study, we evaluated the ... ...

Abstract	Background: Mast cells (MCs) are important effector cells in anaphylaxis and anaphylactic disease. 3',4',5,7-tetrahydroxyflavone (THF) presents in many medicinal plants and exerts a variety of pharmacological effects. In this study, we evaluated the effect of THF on C48/80-induced anaphylaxis and the mechanisms underlying its effects, including the role of secreted phosphoprotein 1 (SPP1), which has not been reported to IgE-independent MC activation. Results: THF inhibited C48/80-induced Ca Conclusions: Our results validated SPP1 is involved in IgE-independent MC activation anaphylactoid reactions. THF inhibited C48/80-mediated anaphylactoid reactions both
MeSH term(s)	Humans ; Anaphylaxis/chemically induced ; Anaphylaxis/drug therapy ; Luteolin/pharmacology ; Osteopontin/metabolism ; Osteopontin/pharmacology ; Mast Cells ; Inflammation/metabolism ; Cell Degranulation ; Immunoglobulin E/metabolism
Chemical Substances	Luteolin (KUX1ZNC9J2) ; Osteopontin (106441-73-0) ; Immunoglobulin E (37341-29-0)
Language	English
Publishing date	2023-07-03
Publishing country	England
Document type	Journal Article
ZDB-ID	807033-7
ISSN	1532-2513 ; 0892-3973
ISSN (online)	1532-2513
ISSN	0892-3973
DOI	10.1080/08923973.2023.2228478
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Zs.A 1481: Show issues

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Article ; Online: Chrysin Inhibits Pseudo-allergic Reaction by Suppressing Mitochondrial STAT3 Activation

Zhang, Yongjing / Xue, Zhuoyin / Hu, Shiling / Bai, Haoyun / Wang, Jue / Wang, Nan

Journal of agricultural and food chemistry

2021 Volume 69, Issue 23, Page(s) 6569–6577

Abstract: Chrysin, one of the most pharmacologically active natural flavonoids, has been extracted from various plants. Mast cells are an important part of innate immunity-mediating anaphylaxis. Pseudo-allergic reactions are currently believed to be associated ... ...

Abstract	Chrysin, one of the most pharmacologically active natural flavonoids, has been extracted from various plants. Mast cells are an important part of innate immunity-mediating anaphylaxis. Pseudo-allergic reactions are currently believed to be associated with the MAS-related GPR family member X2 (MrgX2). In this study, the anti-pseudo allergy effect of chrysin and its underlying mechanisms were studied
MeSH term(s)	Anaphylaxis ; Cell Degranulation ; Flavonoids ; Humans ; Mast Cells ; Receptors, G-Protein-Coupled/genetics
Chemical Substances	Flavonoids ; Receptors, G-Protein-Coupled ; chrysin (3CN01F5ZJ5)
Language	English
Publishing date	2021-06-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.1c02565
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Zs.A 1172: Show issues

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Article: [Detection of drugs in urine by ambient direct ionization mass spectrometry].

Xiong, Shiling / Hong, Huanhuan / Wen, Luhong / Hu, Shundi / Chen, Anqi / Xiong, Wei / Chen, La

Se pu = Chinese journal of chromatography

2022 Volume 40, Issue 7, Page(s) 677–683

Abstract: According to the Report of Drug Situation in China (2020), the growth rate of the number of drug abusers in China has decreased, but the number of drug abusers is still large. An efficient screening method is necessary for controlling drug abuse. As an ... ...

Abstract	According to the Report of Drug Situation in China (2020), the growth rate of the number of drug abusers in China has decreased, but the number of drug abusers is still large. An efficient screening method is necessary for controlling drug abuse. As an important type of biological sample, urine is widely used for the rapid screening of drug addicts. However, because of the complex composition, low content, and strong interference from the body's metabolism, the detection of drugs in urine remains a challenge. Traditional rapid screening techniques such as immunocolloidal gold analysis have a high false positive rate and insufficient quantitative capability. In addition, laboratory mass spectrometry methods require complicated time-consuming sample pre-processing and strict environmental conditions, and hence, are unsuitable for on-site rapid analysis. In recent years, various direct ionization mass spectrometry techniques such as direct analysis in real time (DART), desorption electrospray ionization (DESI), and dielectric barrier discharge ionization (DBDI) have advanced rapidly. These techniques have been applied to public safety, food safety, environmental detection, etc. In contrast to traditional ionization mass spectrometry methods, these direct ionization techniques allow for the
MeSH term(s)	China ; Limit of Detection ; Methamphetamine ; Spectrometry, Mass, Electrospray Ionization/methods
Chemical Substances	Methamphetamine (44RAL3456C)
Language	Chinese
Publishing date	2022-07-05
Publishing country	China
Document type	Journal Article
ISSN	1000-8713
ISSN	1000-8713
DOI	10.3724/SP.J.1123.2022.01013
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Article ; Online: Paeoniflorin inhibits IgE-mediated allergic reactions by suppressing the degranulation of mast cells though binding with FcϵRI alpha subunits.

Zhang, Yongjing / Hu, Shiling / Ge, Shuai / Wang, Jue / He, Langchong

European journal of pharmacology

2020 Volume 886, Page(s) 173415

Abstract: Paeoniflorin (PF), a monoterpene glycoside isolated from the aqueous extract of the Chinese herb Radix Paeoniae Alba, has been used for treating various inflammatory diseases. In this study, we aimed to investigate the anti-allergic activities of PF. The ...

Abstract	Paeoniflorin (PF), a monoterpene glycoside isolated from the aqueous extract of the Chinese herb Radix Paeoniae Alba, has been used for treating various inflammatory diseases. In this study, we aimed to investigate the anti-allergic activities of PF. The anti-anaphylactic activity of PF was investigated using mast cell (MC) degranulation assay as well as Ca
MeSH term(s)	Animals ; Calcium/metabolism ; Cell Degranulation/drug effects ; Chemokines/metabolism ; Dose-Response Relationship, Drug ; Glucosides/pharmacology ; Glucosides/therapeutic use ; Histamine Release/drug effects ; Hypersensitivity/drug therapy ; Hypersensitivity/prevention & control ; Immunoglobulin E/drug effects ; Male ; Mast Cells/drug effects ; Mice ; Mice, Inbred C57BL ; Molecular Docking Simulation ; Monoterpenes/pharmacology ; Monoterpenes/therapeutic use ; Ovalbumin/immunology ; Passive Cutaneous Anaphylaxis/drug effects ; Receptors, IgE/drug effects ; Signal Transduction/drug effects ; Skin/pathology
Chemical Substances	Chemokines ; Glucosides ; Monoterpenes ; Receptors, IgE ; peoniflorin (21AIQ4EV64) ; Immunoglobulin E (37341-29-0) ; Ovalbumin (9006-59-1) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2020-08-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80121-5
ISSN	1879-0712 ; 0014-2999
ISSN (online)	1879-0712
ISSN	0014-2999
DOI	10.1016/j.ejphar.2020.173415
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Zs.A 522: Show issues

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Article ; Online: Antiviral drugs suppress infection of 2019-nCoV spike pseudotyped virus by interacting with ACE2 protein.

Wang, Jue / Zhang, Yongjing / Hu, Shiling / Bai, Haoyun / Xue, Zhuoyin / Liu, Yanhong / Ma, Weina

Journal of biochemical and molecular toxicology

2021 Volume 36, Issue 2, Page(s) e22948

Abstract: The outbreak of coronavirus disease 2019 (COVID-19) has induced a large number of deaths worldwide. Angiotensin-converting enzyme 2 (ACE2) is the entry receptor for the 2019 novel coronavirus (2019-nCoV) to infect the host cells. Therefore, ACE2 may be ... ...

Abstract	The outbreak of coronavirus disease 2019 (COVID-19) has induced a large number of deaths worldwide. Angiotensin-converting enzyme 2 (ACE2) is the entry receptor for the 2019 novel coronavirus (2019-nCoV) to infect the host cells. Therefore, ACE2 may be an important target for the prevention and treatment of COVID-19. The aim of this study was to investigate the inhibition effect of valaciclovir hydrochloride (VACV), zidovudine (ZDV), saquinavir (SQV), and efavirenz (EFV) on 2019-nCoV infection. The results of molecule docking and surface plasmon resonance showed that VACV, ZDV, SQV, and EFV could bind to ACE2 protein, with the K
MeSH term(s)	Allosteric Site ; Antiviral Agents/metabolism ; Antiviral Agents/pharmacology ; COVID-19/drug therapy ; COVID-19/prevention & control ; COVID-19/virology ; HEK293 Cells ; Humans ; Molecular Docking Simulation ; Peptidyl-Dipeptidase A/drug effects ; Peptidyl-Dipeptidase A/metabolism ; Protein Binding ; SARS-CoV-2/drug effects ; Surface Plasmon Resonance ; Viral Pseudotyping
Chemical Substances	Antiviral Agents ; ACE protein, human (EC 3.4.15.1) ; Peptidyl-Dipeptidase A (EC 3.4.15.1)
Language	English
Publishing date	2021-11-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	1410020-4
ISSN	1099-0461 ; 1095-6670
ISSN (online)	1099-0461
ISSN	1095-6670
DOI	10.1002/jbt.22948
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Zs.A 2201: Show issues

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Article ; Online: Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2.

Hu, Shiling / Wang, Jue / Zhang, Yongjing / Bai, Haoyun / Wang, Cheng / Wang, Nan / He, Langchong

Journal of medical virology

2021 Volume 93, Issue 5, Page(s) 3143–3151

Abstract: Since December 2019, the new coronavirus (also known as severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2, 2019-nCoV])-induced disease, COVID-19, has spread rapidly worldwide. Studies have reported that the traditional Chinese medicine Salvia ... ...

Abstract	Since December 2019, the new coronavirus (also known as severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2, 2019-nCoV])-induced disease, COVID-19, has spread rapidly worldwide. Studies have reported that the traditional Chinese medicine Salvia miltiorrhiza possesses remarkable antiviral properties; however, the anti-coronaviral activity of its main components, salvianolic acid A (SAA), salvianolic acid B (SAB), and salvianolic acid C (SAC) is still debated. In this study, we used Cell Counting Kit-8 staining and flow cytometry to evaluate the toxicity of SAA, SAB, and SAC on ACE2 (angiotensin-converting enzyme 2) high-expressing HEK293T cells (ACE2
MeSH term(s)	Alkenes/chemistry ; Alkenes/pharmacology ; Angiotensin-Converting Enzyme 2/chemistry ; Angiotensin-Converting Enzyme 2/metabolism ; Benzofurans/chemistry ; Benzofurans/pharmacology ; COVID-19/drug therapy ; Caffeic Acids/chemistry ; Caffeic Acids/pharmacology ; Cell Survival ; HEK293 Cells ; Humans ; Lactates/chemistry ; Lactates/pharmacology ; Molecular Structure ; Polyphenols/chemistry ; Polyphenols/pharmacology ; Protein Binding ; SARS-CoV-2/drug effects ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/metabolism ; Virus Internalization
Chemical Substances	Alkenes ; Benzofurans ; Caffeic Acids ; Lactates ; Polyphenols ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; salvianolic acid A (51622542XO) ; salvianolic acid B (C1GQ844199) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) ; salvianolic acid C (I16H9Z53ZL)
Language	English
Publishing date	2021-02-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.26874
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Zs.A 1320: Show issues

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Article ; Online: Dictamnine is an effective anti-anaphylactoid compound acting via the MrgX2 receptor located on mast cells.

Liu, Rui / Hu, Shiling / Ding, Yuanyuan / Wang, Jue / Wang, Yuejin / Gao, Jiapan / He, Langchong

Phytotherapy research : PTR

2021 Volume 35, Issue 6, Page(s) 3181–3193

Abstract: Anaphylactoid reactions are potentially fatal allergic diseases caused by mast cells (MCs), which release histamine and lipid mediators under certain stimuli. Therefore, there is an urgent need to develop new drug candidates to treat anaphylactoid ... ...

Abstract	Anaphylactoid reactions are potentially fatal allergic diseases caused by mast cells (MCs), which release histamine and lipid mediators under certain stimuli. Therefore, there is an urgent need to develop new drug candidates to treat anaphylactoid reactions. The MrgX2 receptor mediates anaphylactoid reactions that cause inflammatory diseases. Cortex dictamni is a Chinese herb used for treating allergy-related diseases; however, its active compound is still unknown and its mechanism of action has not yet been reported. The aim of this study was to screen the anti-anaphylactoid compound from C. dictamni extracts. An MrgX2/CMC-HPLC method was established for screening MrgX2-specific compounds retained from the alcohol extract of C. dictamni. A mouse model of hindpaw extravasation was used to evaluate the anti-anaphylactoid effect of this ingredient. Intracellular Ca
MeSH term(s)	Anaphylaxis/drug therapy ; Animals ; Calcium/metabolism ; Cell Degranulation/drug effects ; Chemokines/metabolism ; Cytokines/metabolism ; Disease Models, Animal ; Histamine/metabolism ; Humans ; Male ; Mast Cells/drug effects ; Mice ; Mice, Inbred C57BL ; Nerve Tissue Proteins/metabolism ; Quinolines/pharmacology ; Receptors, G-Protein-Coupled/metabolism ; Receptors, Neuropeptide/metabolism ; Signal Transduction
Chemical Substances	Chemokines ; Cytokines ; MRGPRX2 protein, human ; Nerve Tissue Proteins ; Quinolines ; Receptors, G-Protein-Coupled ; Receptors, Neuropeptide ; Histamine (820484N8I3) ; dictamnine (HQZ3798D0A) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2021-04-24
Publishing country	England
Document type	Journal Article
ZDB-ID	639136-9
ISSN	1099-1573 ; 0951-418X
ISSN (online)	1099-1573
ISSN	0951-418X
DOI	10.1002/ptr.7007
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Zs.B 3092: Show issues

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Article ; Online: Resveratrol inhibits MRGPRX2-mediated mast cell activation via Nrf2 pathway.

Wang, Jue / Zhang, Yongjing / Hu, Shiling / Ge, Shuai / Jia, Min / Wang, Nan

International immunopharmacology

2021 Volume 93, Page(s) 107426

Abstract: Mast cells (MCs) are crucial effectors in inflammation and allergic reactions. The Mas-related G-protein-coupled receptor X2 (MRGPRX2) was the MC-specific receptor and play a key role in IgE-independent allergic reactions. The activation of the Nuclear ... ...

Abstract	Mast cells (MCs) are crucial effectors in inflammation and allergic reactions. The Mas-related G-protein-coupled receptor X2 (MRGPRX2) was the MC-specific receptor and play a key role in IgE-independent allergic reactions. The activation of the Nuclear factor erythroid derived 2-related factor 2 (Nrf2) is involved in IgE-mediated MC degranulation. Resveratrol (Res) is a polyphenolic compound in red wine and has been reported to exert a variety of pharmacological effects. In the current study, we investigated the effect of Res in regulating MRGPRX2-mediated MC activation and its underlyingmechanism. We demonstrated that Res reduced compound 48/80 (C48/80)-induced calcium flux in MCs and inhibited MCs degranulation in vitro. Res also suppressed C48/80-induced hind paw extravasation, active systemic anaphylaxis, and MCs degranulation in mouse models of pseudo-allergy in vivo. Furthermore, PCR and immunohistochemistry assay suggest that Res up-regulates Nrf2 expression and Nrf2 inhibitor attenuates the protective effects of Res. In conclusion, Res exerts an inhibitory effect on MRGPRX2-mediated MCs activation by targeting Nrf2 pathway and may present a promising new therapeutic agent for the treatment of MRGPRX2-dependent anaphylactoid reactions.
MeSH term(s)	Anaphylaxis/etiology ; Anaphylaxis/prevention & control ; Animals ; Anti-Allergic Agents/therapeutic use ; Cell Degranulation ; Cells, Cultured ; Disease Models, Animal ; Hypersensitivity/complications ; Hypersensitivity/drug therapy ; Hypersensitivity/immunology ; Male ; Mast Cells/immunology ; Mice ; Mice, Inbred C57BL ; NF-E2-Related Factor 2/metabolism ; Receptors, G-Protein-Coupled/metabolism ; Resveratrol/therapeutic use ; Signal Transduction
Chemical Substances	Anti-Allergic Agents ; Mrgprx2 protein, mouse ; NF-E2-Related Factor 2 ; Nfe2l2 protein, mouse ; Receptors, G-Protein-Coupled ; Resveratrol (Q369O8926L)
Language	English
Publishing date	2021-02-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2021.107426
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Zs.A 5408: Show issues

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Article: Chrysin Inhibits Pseudo-allergic Reaction by Suppressing Mitochondrial STAT3 Activation via MAS-Related GPR Family Member X2

Zhang, Yongjing / Xue, Zhuoyin / Hu, Shiling / Bai, Haoyun / Wang, Jue / Wang, Nan

Journal of agricultural and food chemistry. 2021 June 08, v. 69, no. 23

2021

Abstract	Chrysin, one of the most pharmacologically active natural flavonoids, has been extracted from various plants. Mast cells are an important part of innate immunity-mediating anaphylaxis. Pseudo-allergic reactions are currently believed to be associated with the MAS-related GPR family member X2 (MrgX2). In this study, the anti-pseudo allergy effect of chrysin and its underlying mechanisms were studied in vitro and in vivo. Chrysin inhibited passive cutaneous anaphylaxis and systemic pseudo-allergy in vivo. LAD2 cell degranulation, calcium ion (Ca²⁺) influx, and adenosine 5′-triphosphate (ATP) content were significantly suppressed in a dose-dependent manner. Chrysin suppressed pseudo-allergic reactions through the PLC/IP3/Ca²⁺ and ERK/STAT3 serine 727 pathways downstream of MrgX2. Therefore, mitochondrial ATP, but not glycolysis, is vital for pseudo-allergic reactions mediated by MrgX2. This study provides new insights for the treatment of pseudo-allergy.
Keywords	adenosine ; anaphylaxis ; calcium ; chrysin ; dose response ; food chemistry ; glycolysis ; medicinal properties ; mitochondria ; serine
Language	English
Dates of publication	2021-0608
Size	p. 6569-6577.
Publishing place	American Chemical Society
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.1c02565
Database	NAL-Catalogue (AGRICOLA)

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Article: Dictamnine is an effective anti‐anaphylactoid compound acting via the MrgX2 receptor located on mast cells

Liu, Rui / Hu, Shiling / Ding, Yuanyuan / Wang, Jue / Wang, Yuejin / Gao, Jiapan / He, Langchong

Phytotherapy research. 2021 June, v. 35, no. 6

2021

Abstract	Anaphylactoid reactions are potentially fatal allergic diseases caused by mast cells (MCs), which release histamine and lipid mediators under certain stimuli. Therefore, there is an urgent need to develop new drug candidates to treat anaphylactoid reactions. The MrgX2 receptor mediates anaphylactoid reactions that cause inflammatory diseases. Cortex dictamni is a Chinese herb used for treating allergy‐related diseases; however, its active compound is still unknown and its mechanism of action has not yet been reported. The aim of this study was to screen the anti‐anaphylactoid compound from C. dictamni extracts. An MrgX2/CMC‐HPLC method was established for screening MrgX2‐specific compounds retained from the alcohol extract of C. dictamni. A mouse model of hindpaw extravasation was used to evaluate the anti‐anaphylactoid effect of this ingredient. Intracellular Ca²⁺ mobilization was assessed using a calcium imaging assay. Enzyme immunoassays were performed to measure cytokine and chemokine release levels. The molecular signaling pathways were explored by western blotting. As a result, dictamnine was identified as an effective compound using the MrgX2/CMC method, which remarkably suppressed MC intracellular Ca²⁺ mobilization and the release of de novo degranulated substances, and inhibited PKC‐PLCγ‐IP3R‐associated protein signaling molecules. Hence, dictamnine is a novel therapeutic candidate for anaphylactoid reactions via MrgX2.
Keywords	active ingredients ; alcohols ; calcium ; chemokines ; cortex ; drugs ; enzymes ; histamine ; lipids ; mechanism of action ; mice ; phytotherapy ; research
Language	English
Dates of publication	2021-06
Size	p. 3181-3193.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	639136-9
ISSN	1099-1573 ; 0951-418X
ISSN (online)	1099-1573
ISSN	0951-418X
DOI	10.1002/ptr.7007
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