LIVIVO - Search results -

Search results

Result 1 - 10 of total 360

Search options

Article ; Online: Forward Genetics in Apicomplexa Biology: The Host Side of the Story.

Sánchez-Arcila, Juan C / Jensen, Kirk D C

Frontiers in cellular and infection microbiology

2022 Volume 12, Page(s) 878475

Abstract: Forward genetic approaches have been widely used in parasitology and have proven their power to reveal the complexities of host-parasite interactions in an unbiased fashion. Many aspects of the parasite's biology, including the identification of ... ...

Abstract	Forward genetic approaches have been widely used in parasitology and have proven their power to reveal the complexities of host-parasite interactions in an unbiased fashion. Many aspects of the parasite's biology, including the identification of virulence factors, replication determinants, antibiotic resistance genes, and other factors required for parasitic life, have been discovered using such strategies. Forward genetic approaches have also been employed to understand host resistance mechanisms to parasitic infection. Here, we will introduce and review all forward genetic approaches that have been used to identify host factors involved with Apicomplexa infections, which include classical genetic screens and QTL mapping, GWAS, ENU mutagenesis, overexpression, RNAi and CRISPR-Cas9 library screens. Collectively, these screens have improved our understanding of host resistance mechanisms, immune regulation, vaccine and drug designs for Apicomplexa parasites. We will also discuss how recent advances in molecular genetics give present opportunities to further explore host-parasite relationships.
MeSH term(s)	Apicomplexa/genetics ; Biology ; Genetic Testing ; Host-Parasite Interactions/genetics ; Mutagenesis
Language	English
Publishing date	2022-05-12
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2022.878475
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Training the Fetal Immune System Through Maternal Inflammation-A Layered Hygiene Hypothesis.

Apostol, April C / Jensen, Kirk D C / Beaudin, Anna E

Frontiers in immunology

2020 Volume 11, Page(s) 123

Abstract: Over the last century, the alarming surge in allergy and autoimmune disease has led to the hypothesis that decreasing exposure to microbes, which has accompanied industrialization and modern life in the Western world, has fundamentally altered the immune ...

Abstract	Over the last century, the alarming surge in allergy and autoimmune disease has led to the hypothesis that decreasing exposure to microbes, which has accompanied industrialization and modern life in the Western world, has fundamentally altered the immune response. In its current iteration, the "hygiene hypothesis" suggests that reduced microbial exposures during
MeSH term(s)	Adult ; Child ; Female ; Fetus/immunology ; Hematopoietic Stem Cells/immunology ; Hematopoietic Stem Cells/microbiology ; Host Microbial Interactions/immunology ; Humans ; Hygiene Hypothesis ; Hypersensitivity/immunology ; Immune System/immunology ; Infant, Newborn ; Inflammation/immunology ; Microbial Interactions/immunology ; Pregnancy ; T-Lymphocytes, Helper-Inducer/immunology
Language	English
Publishing date	2020-02-11
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2020.00123
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Variation in CD8 T cell IFNγ differentiation to strains of

Kongsomboonvech, Angel K / García-López, Laura / Njume, Ferdinand / Rodriguez, Felipe / Souza, Scott P / Rosenberg, Alex / Jensen, Kirk D C

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1130965

Abstract: Introduction: Toxoplasma gondii: Methods: Therefore, we screened F1 progeny from genetic crosses between the clonal lineage strains to identify ROCTR. Naïve antigen-specific CD8 T cells (T57) isolated from transnuclear mice, which are specific for ... ...

Abstract	Introduction: Toxoplasma gondii Methods: Therefore, we screened F1 progeny from genetic crosses between the clonal lineage strains to identify ROCTR. Naïve antigen-specific CD8 T cells (T57) isolated from transnuclear mice, which are specific for the endogenous and vacuolar TGD057 antigen, were measured for their ability to become activated, transcribe Results: Genetic mapping returned four non-interacting quantitative trait loci (QTL) with small effect on Discussion: Collectively, our data suggest that while CD8 T cell IFNγ production to
MeSH term(s)	Animals ; Mice ; Toxoplasma ; Quantitative Trait Loci ; Protozoan Proteins/metabolism ; Interferon-gamma/metabolism ; CD8-Positive T-Lymphocytes ; Cell Differentiation
Chemical Substances	Protozoan Proteins ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2023-05-23
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1130965
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: T cell exhaustion dynamics in systemic autoimmune disease.

Turner, Christi N / Camilo Sanchez Arcila, Juan / Huerta, Noah / Quiguoe, Avi Rae / Jensen, Kirk D C / Hoyer, Katrina K

bioRxiv : the preprint server for biology

2023

Abstract: Unlike in infection and cancer, T cell exhaustion in autoimmune disease has not been clearly defined. Here we set out to understand inhibitory protein (PD-1, Tim3, CTLA4, Lag3) expression in CXCR5- and CXCR5+ CD8 and CD4 T cells in systemic lupus ... ...

Abstract	Unlike in infection and cancer, T cell exhaustion in autoimmune disease has not been clearly defined. Here we set out to understand inhibitory protein (PD-1, Tim3, CTLA4, Lag3) expression in CXCR5- and CXCR5+ CD8 and CD4 T cells in systemic lupus erythematosus. CXCR5+ CD8 and CD4 T cells express PD-1 and engage B cells in germinal center reactions, leading to autoantibody formation in autoimmunity. We hypothesized that CXCR5+ CD8 T cells develop an exhausted phenotype as SLE autoimmunity expands from initial to chronic, self-perpetuating disease due to chronic self-antigen exposure. Our results indicate that there is no exhaustion frequency differences between sexes, although disease kinetics vary by sex. CXCR5+ CD8 T cells express primarily IFNγ, known to promote autoimmune disease development, whereas CXCR5-CD8 T cells express TNFα and IFNγ as disease progresses from 2-6 months. Tim3 is the highest expressed inhibitory marker for all CD4 and CD8 T cell populations demonstrating potential for terminally exhausted populations. CTLA4 expression on CD4 T cells suggests potential tolerance induction in these cells. We identified exhaustion phenotypes within autoimmune disease that progress with increasing lupus erythematosus severity and possibly provide a feedback mechanism for immunological tolerance. Highlights: CXCR5- and CXCR5+ CD8 T cells expand with rate of disease in SLE mouse model.CXCR5+ CD8 T cells are low contributors to TNFα disease progression unlike CXCR5-CD8 T cells but may increase disease mechanisms through high IFNγ production.Inhibitory markers upregulate in frequency with the highest amounts seen in Tim3+ populations. Tim3+Lag3+ expression may be an indicator of terminal differentiation for all populations.Inhibitory marker expression frequency was unrelated to sex.
Language	English
Publishing date	2023-12-23
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.12.23.573167
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Antigen Presentation of Vacuolated Apicomplexans--Two Gateways to a Vaccine Antigen.

Jensen, Kirk D C

Trends in parasitology

2015 Volume 32, Issue 2, Page(s) 88–90

Abstract: For parasites that sequester themselves within a vacuole, new rules governing antigen presentation are coming into focus. Components of the host's autophagy machinery and the parasite's membranous nanotubular network within the parasitophorous vacuole ... ...

Abstract	For parasites that sequester themselves within a vacuole, new rules governing antigen presentation are coming into focus. Components of the host's autophagy machinery and the parasite's membranous nanotubular network within the parasitophorous vacuole play a major role in determining antigenicity of Toxoplasma proteins. As such, both parasite and vaccinologist may exploit these pathways to regulate the ever important CD8 T cell response to apicomplexan parasites.
MeSH term(s)	Animals ; Antigens/immunology ; Apicomplexa/immunology ; Autophagy ; CD8-Positive T-Lymphocytes/immunology ; Humans ; Protozoan Vaccines
Chemical Substances	Antigens ; Protozoan Vaccines
Language	English
Publishing date	2015-12-28
Publishing country	England
Document type	Journal Article
ZDB-ID	2036227-4
ISSN	1471-5007 ; 1471-4922
ISSN (online)	1471-5007
ISSN	1471-4922
DOI	10.1016/j.pt.2015.12.011
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2898: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 16: Show issues
Zs.MO 251: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Antigen Presentation of Vacuolated Apicomplexans – Two Gateways to a Vaccine Antigen

Jensen, Kirk D.C

Trends in parasitology. 2016 Feb., v. 32, no. 2

2016

Abstract	For parasites that sequester themselves within a vacuole, new rules governing antigen presentation are coming into focus. Components of the host's autophagy machinery and the parasite's membranous nanotubular network within the parasitophorous vacuole play a major role in determining antigenicity of Toxoplasma proteins. As such, both parasite and vaccinologist may exploit these pathways to regulate the ever important CD8 T cell response to apicomplexan parasites.
Keywords	antigen presentation ; antigens ; autophagy ; CD8-positive T-lymphocytes ; parasites ; proteins ; Toxoplasma ; vaccines ; vacuoles
Language	English
Dates of publication	2016-02
Size	p. 88-90.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2036227-4
ISSN	1471-5007 ; 1471-4922
ISSN (online)	1471-5007
ISSN	1471-4922
DOI	10.1016/j.pt.2015.12.011
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.B 2898: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 16: Show issues
Zs.MO 251: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Author Correction: Cell-based versus corticosteroid injections for knee pain in osteoarthritis: a randomized phase 3 trial.

Nature medicine

2023 Volume 30, Issue 3, Page(s) 905

Language	English
Publishing date	2023-12-22
Publishing country	United States
Document type	Published Erratum
ZDB-ID	1220066-9
ISSN	1546-170X ; 1078-8956
ISSN (online)	1546-170X
ISSN	1078-8956
DOI	10.1038/s41591-023-02776-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4212: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 39: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: T cell signaling and Treg dysfunction correlate to disease kinetics in IL-2Rα-KO autoimmune mice.

Mullins, Genevieve N / Valentine, Kristen M / Al-Kuhlani, Mufadhal / Davini, Dan / Jensen, Kirk D C / Hoyer, Katrina K

Scientific reports

2020 Volume 10, Issue 1, Page(s) 21994

Abstract: IL-2Rα, in part, comprises the high affinity receptor for IL-2, a cytokine important in immune proliferation, activation, and regulation. IL-2Rα deficient mice (IL-2Rα-KO) develop systemic autoimmune disease and die from severe anemia between 18 and 80 ... ...

Abstract	IL-2Rα, in part, comprises the high affinity receptor for IL-2, a cytokine important in immune proliferation, activation, and regulation. IL-2Rα deficient mice (IL-2Rα-KO) develop systemic autoimmune disease and die from severe anemia between 18 and 80 days of age. These mice develop kinetically distinct autoimmune progression, with approximately a quarter dying by 21 days of age and half dying after 30 days. This research aims to define immune parameters and cytokine signaling that distinguish cohorts of IL-2Rα-KO mice that develop early- versus late-stage autoimmune disease. To investigate these differences, we evaluated complete blood counts (CBC), antibody binding of RBCs, T cell numbers and activation, hematopoietic progenitor changes, and signaling kinetics, during autoimmune hemolytic anemia (AIHA) and bone marrow failure. We identified several alterations that, when combined, correlate to disease kinetics. Early onset disease correlates with anti-RBC antibodies, lower hematocrit, and reduced IL-7 signaling. CD8 regulatory T cells (Tregs) have enhanced apoptosis in early disease. Further, early and late end stage disease, while largely similar, had several differences suggesting distinct mechanisms drive autoimmune disease kinetics. Therefore, IL-2Rα-KO disease pathology rates, driven by T cell signaling, promote effector T cell activation and expansion and Treg dysfunction.
MeSH term(s)	Animals ; Apoptosis ; CD8-Positive T-Lymphocytes/immunology ; Cell Proliferation ; Erythrocytes/metabolism ; Immunologic Memory ; Interleukin-2/metabolism ; Interleukin-2 Receptor alpha Subunit/deficiency ; Interleukin-2 Receptor alpha Subunit/metabolism ; Kinetics ; Lymphocyte Activation/immunology ; Mice, Inbred BALB C ; Mice, Knockout ; Signal Transduction ; T-Lymphocytes, Regulatory/immunology ; Thymus Gland/growth & development
Chemical Substances	Interleukin-2 ; Interleukin-2 Receptor alpha Subunit
Language	English
Publishing date	2020-12-15
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-78975-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Cell-based versus corticosteroid injections for knee pain in osteoarthritis: a randomized phase 3 trial.

Nature medicine

2023 Volume 29, Issue 12, Page(s) 3120–3126

Abstract: Various types of cellular injection have become a popular and costly treatment option for patients with knee osteoarthritis despite a paucity of literature establishing relative efficacy to each other or corticosteroid injections. Here we aimed to ... ...

Abstract	Various types of cellular injection have become a popular and costly treatment option for patients with knee osteoarthritis despite a paucity of literature establishing relative efficacy to each other or corticosteroid injections. Here we aimed to identify the safety and efficacy of cell injections from autologous bone marrow aspirate concentrate, autologous adipose stromal vascular fraction and allogeneic human umbilical cord tissue-derived mesenchymal stromal cells, in comparison to corticosteroid injection (CSI). The study was a phase 2/3, four-arm parallel, multicenter, single-blind, randomized, controlled clinical trial with 480 patients with a diagnosis of knee osteoarthritis (Kellgren-Lawrence II-IV). Participants were randomized to the three different arms with a 3:1 distribution. Arm 1: autologous bone marrow aspirate concentrate (n = 120), CSI (n = 40); arm 2: umbilical cord tissue-derived mesenchymal stromal cells (n = 120), CSI (n = 40); arm 3: stromal vascular fraction (n = 120), CSI (n = 40). The co-primary endpoints were the visual analog scale pain score and Knee injury and Osteoarthritis Outcome Score pain score at 12 months versus baseline. Analyses of our primary endpoints, with 440 patients, revealed that at 1 year post injection, none of the three orthobiologic injections was superior to another, or to the CSI control. In addition, none of the four groups showed a significant change in magnetic resonance imaging osteoarthritis score compared to baseline. No procedure-related serious adverse events were reported during the study period. In summary, this study shows that at 1 year post injection, there was no superior orthobiologic as compared to CSI for knee osteoarthritis. ClinicalTrials.gov Identifier: NCT03818737.
MeSH term(s)	Humans ; Osteoarthritis, Knee/complications ; Osteoarthritis, Knee/diagnostic imaging ; Osteoarthritis, Knee/drug therapy ; Pain/drug therapy ; Pain/etiology ; Single-Blind Method ; Treatment Outcome
Language	English
Publishing date	2023-11-02
Publishing country	United States
Document type	Randomized Controlled Trial ; Multicenter Study ; Clinical Trial, Phase II ; Clinical Trial, Phase III ; Journal Article
ZDB-ID	1220066-9
ISSN	1546-170X ; 1078-8956
ISSN (online)	1546-170X
ISSN	1078-8956
DOI	10.1038/s41591-023-02632-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4212: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 39: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Genetic mapping reveals Nfkbid as a central regulator of humoral immunity to Toxoplasma gondii.

Souza, Scott P / Splitt, Samantha D / Sànchez-Arcila, Juan C / Alvarez, Julia A / Wilson, Jessica N / Wizzard, Safuwra / Luo, Zheng / Baumgarth, Nicole / Jensen, Kirk D C

PLoS pathogens

2021 Volume 17, Issue 12, Page(s) e1010081

Abstract: Protective immunity to parasitic infections has been difficult to elicit by vaccines. Among parasites that evade vaccine-induced immunity is Toxoplasma gondii, which causes lethal secondary infections in chronically infected mice. Here we report that ... ...

Abstract	Protective immunity to parasitic infections has been difficult to elicit by vaccines. Among parasites that evade vaccine-induced immunity is Toxoplasma gondii, which causes lethal secondary infections in chronically infected mice. Here we report that unlike susceptible C57BL/6J mice, A/J mice were highly resistant to secondary infection. To identify correlates of immunity, we utilized forward genetics to identify Nfkbid, a nuclear regulator of NF-κB that is required for B cell activation and B-1 cell development. Nfkbid-null mice ("bumble") did not generate parasite-specific IgM and lacked robust parasite-specific IgG, which correlated with defects in B-2 cell maturation and class-switch recombination. Though high-affinity antibodies were B-2 derived, transfer of B-1 cells partially rescued the immunity defects observed in bumble mice and were required for 100% vaccine efficacy in bone marrow chimeric mice. Immunity in resistant mice correlated with robust isotype class-switching in both B cell lineages, which can be fine-tuned by Nfkbid gene expression. We propose a model whereby humoral immunity to T. gondii is regulated by Nfkbid and requires B-1 and B-2 cells for full protection.
MeSH term(s)	Animals ; B-Lymphocytes/immunology ; Disease Susceptibility/immunology ; I-kappa B Proteins/immunology ; Immunity, Humoral/immunology ; Mice ; Toxoplasma ; Toxoplasmosis, Animal/immunology
Chemical Substances	I-kappa B Proteins ; Nfkbid protein, mouse
Language	English
Publishing date	2021-12-06
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7374
ISSN (online)	1553-7374
ISSN	1553-7374
DOI	10.1371/journal.ppat.1010081
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito