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Article ; Online: Induction of G₂/M arrest, caspase activation and apoptosis by α-santonin derivatives in HL-60 cells.

Ferreira, José Roberto Oliveira / Cavalcanti, Bruno Coêlho / da Costa, Patricia Marçal / de Arantes, Francisco Frederico Perlinson / de Alvarenga, Elson Santiago / Maltha, Célia Regina Alvares / de Almeida Barbosa, Luiz Cláudio / Militão, Gardenia Carmen Gadelha / Pessoa, Claúdia / Ferreira, Paulo Michel Pinheiro

Toxicology in vitro : an international journal published in association with BIBRA

2013 Volume 27, Issue 5, Page(s) 1458–1466

Abstract: ... a significant inhibition on cell-cycle progression and leading to an increasing of cells in G2/M [7.6% and 9.0 ... all compounds caused G2/M reduction and a significant DNA fragmentation. Compounds 2, 3 and 4 were able ...

Abstract	Sesquiterpene lactones (SLs) are natural products with a variety of biological activities. Previously, we demonstrated the cytotoxic effects of three new α-santonin derivatives on different tumor cell lines with low toxic effects upon peripheral human leukocytes. Here, we evaluated the mechanism of action triggered by these derivatives. HL-60 cell cycle determined after 24h treatment revealed a significant inhibition on cell-cycle progression and leading to an increasing of cells in G2/M [7.6% and 9.0% for compound 3% and 9.0% and 8.6% for compound 4 (1 and 2 μM, respectively)]. However, after 48 h exposure, all compounds caused G2/M reduction and a significant DNA fragmentation. Compounds 2, 3 and 4 were able to induce apoptosis on leukemia cells, which was corroborated by phosphatidyserine externalization and activation of caspases-3 and -7 after 24h exposure. None of the derivatives analyzed caused depolarization of mitochondrial membrane within 24h of incubation, suggesting the involvement of the extrinsic apoptotic pathway in the death process. The antiproliferative action of these compounds is related to the DNA synthesis inhibition and cell cycle arrest, which probably lead to apoptosis activation. Therefore, these santonin derivatives are promising lead candidates for development of new cytotoxic agents.
MeSH term(s)	Apoptosis/drug effects ; Caspase 3/metabolism ; Caspase 7/metabolism ; Cell Cycle Checkpoints/drug effects ; Cell Proliferation/drug effects ; Cytotoxins/pharmacology ; DNA Fragmentation ; G2 Phase ; HL-60 Cells ; Humans ; Membrane Potential, Mitochondrial/drug effects ; Santonin/analogs & derivatives ; Santonin/pharmacology
Chemical Substances	Cytotoxins ; Santonin (1VL8J38ERO) ; Caspase 3 (EC 3.4.22.-) ; Caspase 7 (EC 3.4.22.-)
Language	English
Publishing date	2013-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639064-x
ISSN	1879-3177 ; 0887-2333
ISSN (online)	1879-3177
ISSN	0887-2333
DOI	10.1016/j.tiv.2013.03.010
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Zs.A 2223: Show issues

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Book ; Online: As teorias e o caso

Batista, Mariana / Ribeiro, Ednaldo / Arantes, Rogério

2021

Keywords	Political ideologies
Language	Portuguese
Size	1 electronic resource (568 pages)
Publisher	Editora Universidade Federal do ABC
Document type	Book ; Online
Note	Portuguese
HBZ-ID	HT030379246
ISBN	9786589992028 ; 6589992029
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article: Induction of G₂/M arrest, caspase activation and apoptosis by α-santonin derivatives in HL-60 cells

Toxicology in vitro. 2013 Aug., v. 27, no. 5

2013

Abstract: ... a significant inhibition on cell-cycle progression and leading to an increasing of cells in G₂/M [7.6% and 9.0 ... all compounds caused G₂/M reduction and a significant DNA fragmentation. Compounds 2, 3 and 4 were able ...

Abstract	Sesquiterpene lactones (SLs) are natural products with a variety of biological activities. Previously, we demonstrated the cytotoxic effects of three new α-santonin derivatives on different tumor cell lines with low toxic effects upon peripheral human leukocytes. Here, we evaluated the mechanism of action triggered by these derivatives. HL-60 cell cycle determined after 24h treatment revealed a significant inhibition on cell-cycle progression and leading to an increasing of cells in G₂/M [7.6% and 9.0% for compound 3% and 9.0% and 8.6% for compound 4 (1 and 2μM, respectively)]. However, after 48h exposure, all compounds caused G₂/M reduction and a significant DNA fragmentation. Compounds 2, 3 and 4 were able to induce apoptosis on leukemia cells, which was corroborated by phosphatidyserine externalization and activation of caspases-3 and -7 after 24h exposure. None of the derivatives analyzed caused depolarization of mitochondrial membrane within 24h of incubation, suggesting the involvement of the extrinsic apoptotic pathway in the death process. The antiproliferative action of these compounds is related to the DNA synthesis inhibition and cell cycle arrest, which probably lead to apoptosis activation. Therefore, these santonin derivatives are promising lead candidates for development of new cytotoxic agents.
Keywords	DNA ; DNA fragmentation ; apoptosis ; caspases ; cell cycle ; cytotoxicity ; humans ; leukemia ; leukocytes ; mechanism of action ; mitochondrial membrane ; neoplasm cells ; sesquiterpenoid lactones
Language	English
Dates of publication	2013-08
Size	p. 1458-1466.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	639064-x
ISSN	1879-3177 ; 0887-2333
ISSN (online)	1879-3177
ISSN	0887-2333
DOI	10.1016/j.tiv.2013.03.010
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 2223: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Dissecting Reaction Mechanisms and Catalytic Contributions in Flavoprotein Fumarate Reductases.

Curtolo, Felipe / Arantes, Guilherme M

Journal of chemical information and modeling

2023 Volume 63, Issue 11, Page(s) 3510–3520

Abstract: The interconversion between fumarate and succinate is fundamental to the energy metabolism of nearly all organisms. This redox reaction is catalyzed by a large family of enzymes, fumarate reductases and succinate dehydrogenases, using hydride and proton ... ...

Abstract	The interconversion between fumarate and succinate is fundamental to the energy metabolism of nearly all organisms. This redox reaction is catalyzed by a large family of enzymes, fumarate reductases and succinate dehydrogenases, using hydride and proton transfers from a flavin cofactor and a conserved Arg side-chain. These flavoenzymes also have substantial biomedical and biotechnological importance. Therefore, a detailed understanding of their catalytic mechanisms is valuable. Here, calibrated electronic structure calculations in a cluster model of the active site of the Fcc
MeSH term(s)	Protons ; Oxidation-Reduction ; Catalysis ; Succinates ; Fumarates/metabolism ; Flavins/metabolism ; Kinetics
Chemical Substances	Protons ; Succinates ; Fumarates ; Flavins
Language	English
Publishing date	2023-05-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	190019-5
ISSN	1549-960X ; 0095-2338
ISSN (online)	1549-960X
ISSN	0095-2338
DOI	10.1021/acs.jcim.3c00292
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Zs.A 1230: Show issues

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Article ; Online: Endoglucanase pretreatment aids in isolating tailored-cellulose nanofibrils combining energy saving and high-performance packaging.

Las-Casas, Bruno / Arantes, Valdeir

International journal of biological macromolecules

2023 Volume 242, Issue Pt 4, Page(s) 125057

Abstract: ... oxygen transmission rate (1.00 - 3.17 cc·m ...

Abstract	Cellulose nanofibrils (CNFs) have emerged as a potential alternative to synthetic polymers in packaging applications owing to their oxygen and grease barrier performance, as well as their strong mechanical properties. However, the performance of CNF films relies on the inherent characteristics of fibers, which undergo changes during the CNF isolation process. Understanding these variations in characteristics during CNF isolation is crucial for tailoring CNF film properties to achieve optimum performance in packaging applications. In this study, CNFs were isolated by endoglucanase-assisted mechanical ultra-refining. The alterations in the intrinsic characteristics of CNFs and their impact on CNF films were systematically investigated by considering the degree of defibrillation, enzyme loading, and reaction time through a design of experiments. Enzyme loading had a significant influence on the crystallinity index, crystallite size, surface area, and viscosity. Meanwhile, the degree of defibrillation greatly affected the aspect ratio, degree of polymerization, and particle size. CNF films prepared from CNFs isolated under two optimized scenarios (casting and coating applications) exhibited remarkable properties, including high thermal stability (approximately 300 °C), high tensile strength (104 - 113 MPa), excellent oil resistance (kit n°12), and low oxygen transmission rate (1.00 - 3.17 cc·m
MeSH term(s)	Cellulose ; Cellulase ; Product Packaging ; Tensile Strength ; Oxygen ; Nanofibers
Chemical Substances	Cellulose (9004-34-6) ; Cellulase (EC 3.2.1.4) ; Oxygen (S88TT14065)
Language	English
Publishing date	2023-05-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.125057
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Zs.B 2374: Show issues

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Article ; Online: Molecular properties and tautomeric equilibria of isolated flavins.

Curtolo, Felipe / Arantes, Guilherme M

Journal of computational chemistry

2022 Volume 43, Issue 23, Page(s) 1561–1572

Abstract: Flavins are employed as redox cofactors and chromophores in a plethora of flavoenzymes. Their versatility is an outcome of intrinsic molecular properties of the isoalloxazine ring modulated by the protein scaffold and surrounding solvent. Thus, an ... ...

Abstract	Flavins are employed as redox cofactors and chromophores in a plethora of flavoenzymes. Their versatility is an outcome of intrinsic molecular properties of the isoalloxazine ring modulated by the protein scaffold and surrounding solvent. Thus, an investigation of isolated flavins with high-level electronic-structure methods and with error assessment of the calculated properties will contribute to building better models of flavin reactivity. Here, we benchmarked ground-state properties such as electron affinity, gas-phase basicity, dipole moment, torsion energy, and tautomer stability for lumiflavins in all biologically relevant oxidation and charge states. Overall, multiconfigurational effects are small and chemical accuracy is achieved by coupled-cluster treatments of energetic properties. Augmented basis sets and extrapolations to the complete basis-set limit are necessary for consistent agreement with experimental energetics. Among DFT functionals tested, M06-2X shows the best performance for most properties, except gas-phase basicity, in which M06 and CAM-B3LYP perform better. Moreover, dipole moments of radical flavins show large deviations for all functionals studied. Tautomers with noncanonical protonation states are significantly populated at normal temperatures, adding to the complexity of modeling flavins. These results will guide future computational studies of flavoproteins and flavin chemistry by indicating the limitations of electronic-structure methodologies and the contributions of multiple tautomeric states.
MeSH term(s)	Flavins/chemistry ; Oxidation-Reduction
Chemical Substances	Flavins
Language	English
Publishing date	2022-07-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1479181-X
ISSN	1096-987X ; 0192-8651
ISSN (online)	1096-987X
ISSN	0192-8651
DOI	10.1002/jcc.26957
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Article ; Online: Mapping research paths and perspectives over the fieldwork of human physiology in Antarctica: reflections on the integration of science, environment, and subjectivity.

Marques, Alice L / Moraes, Michele M / Arantes, Rosa M E

Anais da Academia Brasileira de Ciencias

2022 Volume 94, Issue suppl 1, Page(s) e20210396

Abstract: We summarize and elaborate on the challenges of researching in the field of human health in Antarctica based on the conceptual and methodological specificities of a line of investigation that aims to study the human presence in Antarctica in all of its ... ...

Abstract	We summarize and elaborate on the challenges of researching in the field of human health in Antarctica based on the conceptual and methodological specificities of a line of investigation that aims to study the human presence in Antarctica in all of its dimensions (biological, psychological, and socio-anthropological). Herein, we discuss the principal results and limitations of the research carried out by researchers of MEDIANTAR (Antarctic medicine, physiology, and anthropology) group of Programa Antártico Brasileiro in isolated, confined, and extreme environments over the last six years. Fieldwork has been carried out in remote research camps, Brazilian navy ships (Almirante Maximiano/H-41 and Ary Rongel/H-44), and Comandante Ferraz Antarctic Station. Adaptative responses to isolated, confined, and extreme environments were studied based on questionnaires, interviews, participative observation, biological samples, anthropometric, and physiological parameters. Our researchers face the unique situation of concomitantly working under the stressful living conditions that are the object of their investigation. A critical examination of the socio-methodological characteristics and challenges of this research niche indicates the need for exchanging the lessons learned and limitations of these practices with researchers in the humanities field, with attention to the human resources needs in multidisciplinary human-related studies.
MeSH term(s)	Adaptation, Physiological ; Antarctic Regions ; Brazil ; Extreme Environments ; Humans ; Research Personnel
Language	English
Publishing date	2022-02-18
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2046885-4
ISSN	1678-2690 ; 0001-3765
ISSN (online)	1678-2690
ISSN	0001-3765
DOI	10.1590/0001-3765202220210396
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The great discovery of DOACs and why physicians insist on misusing it: A paradox of the 21 century.

Arantes, Flávia Bittar B / Furtado, Remo H M

International journal of cardiology

2022 Volume 364, Page(s) 124–125

MeSH term(s)	Administration, Oral ; Anticoagulants ; Humans ; Physicians
Chemical Substances	Anticoagulants
Language	English
Publishing date	2022-06-02
Publishing country	Netherlands
Document type	Editorial ; Comment
ZDB-ID	779519-1
ISSN	1874-1754 ; 0167-5273
ISSN (online)	1874-1754
ISSN	0167-5273
DOI	10.1016/j.ijcard.2022.05.061
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Zs.A 1673: Show issues

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Article: Meningioma Presenting With Intratumoral Hemorrhage on Active Surveillance.

Matias, Rafael / Arantes, Mavilde / Azevedo, Júlia / Jacome, Manuel / Aguiar, Artur

Cureus

2023 Volume 15, Issue 7, Page(s) e41787

Abstract: Meningiomas are relatively common primary adult brain tumors. They are slow-growing, highly vascular, and graded according to histology, phenotypic and genotypic features. We present a case of a 66-year-old male with a history of tongue squamous cell ... ...

Abstract	Meningiomas are relatively common primary adult brain tumors. They are slow-growing, highly vascular, and graded according to histology, phenotypic and genotypic features. We present a case of a 66-year-old male with a history of tongue squamous cell carcinoma, which presented multiple risk factors for cardiovascular and thromboembolic events. A brain lesion was initially detected on a computed tomography (CT) scan and later characterized by magnetic resonance imaging (MRI). The multidisciplinary team decided to maintain surveillance due to the lack of associated symptoms. Upon expansion in size and acute intralesional hemorrhage seen on follow-up imaging, the patient was submitted to surgical excision. The histopathological testing determined it to be an atypical meningioma. Two months later, the patient received stereotactic radiotherapy, and a post-surgical MRI showed no evidence of tumor recurrence. This case report describes a rare occurrence of intratumoral hemorrhage in a meningioma during surveillance, highlighting the importance of vigilant monitoring and consideration of potential risk factors for hemorrhagic events.
Language	English
Publishing date	2023-07-12
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.41787
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Sclerosing Epithelioid Fibrosarcoma of the Spine: Diagnosis and Treatment of a Rare Entity.

Lima, João / Coutada, Andreia / Afonso, Mariana / Aguiar, Artur / Arantes, Mavilde

Cureus

2023 Volume 15, Issue 7, Page(s) e42143

Abstract: Sclerosing epithelioid fibrosarcoma (SEF) is a rare subtype of sarcoma with high rates of local recurrence and distant metastasis. Morphologically, it resembles other mesenchymal and non-mesenchymal tumors, making it a diagnostic challenge. Treatment ... ...

Abstract	Sclerosing epithelioid fibrosarcoma (SEF) is a rare subtype of sarcoma with high rates of local recurrence and distant metastasis. Morphologically, it resembles other mesenchymal and non-mesenchymal tumors, making it a diagnostic challenge. Treatment relies mostly on surgery with adjuvant chemotherapy or radiotherapy (RT). A 46-year-old woman who presented with lumbar pain and weight loss underwent a computed tomography (CT) scan, magnetic resonance imaging (MRI), and a [18F]-fluorodeoxyglucose positron emission tomography-computed tomography (18F-FDG PET/CT) scan, which showed a lesion involving the L5 vertebra. An incisional biopsy of the lesion established the diagnosis of SEF, with diffuse expression of MUC4 and focal expression of EMA. The patient was treated with neoadjuvant RT followed by surgery. Histology was congruent with the previous diagnosis and demonstrated post-radiation changes. In conclusion, SEF is an aggressive type of sarcoma that is easily misdiagnosed, so it is important to consider it in the differential diagnosis to avoid unbeneficial treatments and a detriment to patient survival.
Language	English
Publishing date	2023-07-19
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.42143
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