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  1. Article ; Online: Measurement of SQSTM1 by flow cytometry.

    Hargarten, Jessica C / Hu, Guowu / Elsegeiny, Waleed / Williamson, Peter R

    Autophagy

    2023  Volume 19, Issue 10, Page(s) 2789–2799

    Abstract: Macroautophagy/autophagy is a regulated cellular degradation process essential as a pro-survival mechanism and integral to the regulation of diverse cellular processes in eukaryotes. During cellular stress and nutrient sensing, SQSTM1/p62 (sequestosome 1) ...

    Abstract Macroautophagy/autophagy is a regulated cellular degradation process essential as a pro-survival mechanism and integral to the regulation of diverse cellular processes in eukaryotes. During cellular stress and nutrient sensing, SQSTM1/p62 (sequestosome 1) functions as a key receptor for selective autophagy by shuttling ubiquitinated cargoes toward autophagic degradation making it a useful marker for monitoring autophagic flux. We present a straightforward and rapid flow cytometric assay for the quantitative measurement of intracellular SQSTM1 with improved sensitivity to conventional immunoblotting and with the benefit of higher throughput and reduced requirements for starting cellular materials for adequate analysis. We demonstrate that flow cytometry is able to detect similar trends in the measurement of intracellular SQSTM1 levels following serum starvation, genetic manipulations, and bafilomycin A
    MeSH term(s) Humans ; Mice ; Animals ; Sequestosome-1 Protein/metabolism ; Autophagy/physiology ; Leukocytes, Mononuclear/metabolism ; Flow Cytometry ; Epstein-Barr Virus Infections ; Herpesvirus 4, Human ; Transcription Factors/metabolism
    Chemical Substances Sequestosome-1 Protein ; Transcription Factors ; SQSTM1 protein, human
    Language English
    Publishing date 2023-06-19
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2454135-7
    ISSN 1554-8635 ; 1554-8627
    ISSN (online) 1554-8635
    ISSN 1554-8627
    DOI 10.1080/15548627.2023.2224074
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6741: Show issues Location:
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  2. Article ; Online: Fabrication and characterization of a multi-functional GBR membrane of gelatin-chitosan for osteogenesis and angiogenesis.

    Hu, Xiaofei / He, Yuzhu / Tong, Yunmeng / Sun, Na / Ma, Guowu / Liu, Huiying / Kou, Ni

    International journal of biological macromolecules

    2024  Volume 266, Issue Pt 2, Page(s) 130978

    Abstract: Guided bone regeneration (GBR) membranes are widely used to treat bone defects. In this study, sequential electrospinning and electrospraying techniques were used to prepare a dual-layer GBR membrane composed of gelatin (Gel) and chitosan (CS) containing ...

    Abstract Guided bone regeneration (GBR) membranes are widely used to treat bone defects. In this study, sequential electrospinning and electrospraying techniques were used to prepare a dual-layer GBR membrane composed of gelatin (Gel) and chitosan (CS) containing simvastatin (Sim)-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres (Sim@PLGA/Gel-CS). As a GBR membrane, Sim@PLGA/Gel-CS could act as a barrier to prevent soft tissue from occupying regions of bone tissue. Furthermore, compared with traditional GBR membranes, Sim@PLGA/Gel-CS played an active role on stimulating osteogenesis and angiogenesis. Determination of the physical, chemical, and biological properties of Sim@PLGA/Gel-CS membranes revealed uniform sizes of the nanofibers and microspheres and appropriate morphologies. Fourier-transform infrared spectroscopy was used to characterize the interactions between Sim@PLGA/Gel-CS molecules and the increase in the number of amide groups in crosslinked membranes. The thermal stability and tensile strength of the membranes increased after N-(3-dimethylaminopropyl)-N9- ethylcarbodiimide/N-hydroxysuccinimide crosslinking. The increased fiber density of the barrier layer decreased fibroblast migration compared with that in the osteogenic layer. Osteogenic function was indicated by the increased alkaline phosphatase activity, calcium deposition, and neovascularization. In conclusion, the multifunctional effects of Sim@PLGA/Gel-CS on the barrier and bone microenvironment were achieved via its dual-layer structure and simvastatin coating. Sim@PLGA/Gel-CS has potential applications in bone tissue regeneration.
    MeSH term(s) Chitosan/chemistry ; Gelatin/chemistry ; Osteogenesis/drug effects ; Neovascularization, Physiologic/drug effects ; Membranes, Artificial ; Simvastatin/chemistry ; Simvastatin/pharmacology ; Bone Regeneration/drug effects ; Guided Tissue Regeneration/methods ; Polylactic Acid-Polyglycolic Acid Copolymer/chemistry ; Humans ; Animals ; Tissue Scaffolds/chemistry ; Nanofibers/chemistry ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Microspheres ; Angiogenesis
    Chemical Substances Chitosan (9012-76-4) ; Gelatin (9000-70-8) ; Membranes, Artificial ; Simvastatin (AGG2FN16EV) ; Polylactic Acid-Polyglycolic Acid Copolymer (1SIA8062RS) ; Biocompatible Materials
    Language English
    Publishing date 2024-03-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.130978
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
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  3. Article ; Online: Semantic Consistent Embedding for Domain Adaptive Zero-Shot Learning.

    Zhang, Jianyang / Yang, Guowu / Hu, Ping / Lin, Guosheng / Lv, Fengmao

    IEEE transactions on image processing : a publication of the IEEE Signal Processing Society

    2023  Volume 32, Page(s) 4024–4035

    Abstract: Unsupervised domain adaptation has limitations when encountering label discrepancy between the source and target domains. While open-set domain adaptation approaches can address situations when the target domain has additional categories, these methods ... ...

    Abstract Unsupervised domain adaptation has limitations when encountering label discrepancy between the source and target domains. While open-set domain adaptation approaches can address situations when the target domain has additional categories, these methods can only detect them but not further classify them. In this paper, we focus on a more challenging setting dubbed Domain Adaptive Zero-Shot Learning (DAZSL), which uses semantic embeddings of class tags as the bridge between seen and unseen classes to learn the classifier for recognizing all categories in the target domain when only the supervision of seen categories in the source domain is available. The main challenge of DAZSL is to perform knowledge transfer across categories and domain styles simultaneously. To this end, we propose a novel end-to-end learning mechanism dubbed Three-way Semantic Consistent Embedding (TSCE) to embed the source domain, target domain, and semantic space into a shared space. Specifically, TSCE learns domain-irrelevant categorical prototypes from the semantic embedding of class tags and uses them as the pivots of the shared space. The source domain features are aligned with the prototypes via their supervised information. On the other hand, the mutual information maximization mechanism is introduced to push the target domain features and prototypes towards each other. By this way, our approach can align domain differences between source and target images, as well as promote knowledge transfer towards unseen classes. Moreover, as there is no supervision in the target domain, the shared space may suffer from the catastrophic forgetting problem. Hence, we further propose a ranking-based embedding alignment mechanism to maintain the consistency between the semantic space and the shared space. Experimental results on both I2AwA and I2WebV clearly validate the effectiveness of our method. Code is available at https://github.com/tiggers23/TSCE-Domain-Adaptive-Zero-Shot-Learning.
    Language English
    Publishing date 2023-07-19
    Publishing country United States
    Document type Journal Article
    ISSN 1941-0042
    ISSN (online) 1941-0042
    DOI 10.1109/TIP.2023.3293769
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Low-noise mode-locking in a GHz repetition rate Tm

    Liang, Zhaoheng / Lin, Wei / Wang, Wenlong / Hu, Xu / Pan, Minjie / Fan, Yiheng / Wei, Chiyi / Wang, Luyi / Tang, Guowu / Wei, Xiaoming / Yang, Zhongmin

    Optics letters

    2024  Volume 49, Issue 2, Page(s) 403–406

    Abstract: We demonstrate a GHz repetition rate mode-locked ... ...

    Abstract We demonstrate a GHz repetition rate mode-locked Tm
    Language English
    Publishing date 2024-01-10
    Publishing country United States
    Document type Journal Article
    ISSN 1539-4794
    ISSN (online) 1539-4794
    DOI 10.1364/OL.511218
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Reciprocal polarization imaging of optical activity in reflection.

    Xie, Zhineng / Zhu, Mengjiao / Lin, Weihao / Huang, Guowu / Ge, Yifan / Hu, Yansen / Jin, Xin / Xu, Min

    Optics letters

    2024  Volume 49, Issue 9, Page(s) 2349–2352

    Abstract: We present reciprocal polarization imaging for the optical activity of chiral media in reflection geometry. The method is based on the reciprocal polar decomposition of backscattering Mueller matrices accounting for the reciprocity of light waves in ... ...

    Abstract We present reciprocal polarization imaging for the optical activity of chiral media in reflection geometry. The method is based on the reciprocal polar decomposition of backscattering Mueller matrices accounting for the reciprocity of light waves in forward and backward scattering paths. Anisotropic depolarization is introduced to gain sensitivity to optical activity in backscattering. Experiments with glucose solutions show that while the Lu-Chipman decomposition of the backscattering Mueller matrices produces erroneous results, reciprocal polarization imaging correctly retrieves the optical activity of chiral media. The recovered optical rotation agrees with that obtained in the forward geometry and increases linearly with the concentration and thickness of the chiral media. The potential for in vivo glucose monitoring based on optical activity sensing using reciprocal polarization imaging is then discussed.
    Language English
    Publishing date 2024-05-01
    Publishing country United States
    Document type Journal Article
    ISSN 1539-4794
    ISSN (online) 1539-4794
    DOI 10.1364/OL.516468
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Dynamic changes in PSA levels predict prognostic outcomes in prostate cancer patients undergoing androgen -deprivation therapy: A multicenter retrospective analysis.

    Hu, Mingqiu / Mao, Yifeng / Guan, Chao / Tang, Zhizhong / Bao, Zhihang / Li, Yingbang / Liang, Guowu

    Frontiers in oncology

    2023  Volume 13, Page(s) 1047388

    Abstract: Background: Androgen-deprivation therapy (ADT) is used for the treatment of prostate cancer. However, the specific risk factors for the development of castration-resistant disease are still unclear. The present study sought to identify predictors of ... ...

    Abstract Background: Androgen-deprivation therapy (ADT) is used for the treatment of prostate cancer. However, the specific risk factors for the development of castration-resistant disease are still unclear. The present study sought to identify predictors of patient prognostic outcomes through analyses of clinical findings in large numbers of prostate cancer patients following ADT treatment.
    Methods: Data pertaining to 163 prostate cancer patients treated at the Second Affiliated Hospital of Bengbu Medical University and Maoming People's Hospital from January 1, 2015, to December 30, 2020, were retrospectively analyzed. Dynamic changes in prostate-specific antigen (PSA) levels were regularly assessed, including both time to nadir (TTN) and nadir PSA (nPSA). Univariate and multivariate analyses were performed with Cox risk proportional regression models, while differences in biochemical progression-free survival (bPFS) were compared among groups with Kaplan-Meier curves and log-rank tests.
    Results: The bPFS values over the median 43.5-month follow-up period differed significantly between patients with nPSA levels < 0.2 ng/mL and ≥ 0.2 ng/mL, being 27.6 months and 13.5 months, respectively (log-rank P < 0.001). A significant difference in median bPFS was also observed when comparing patients with a TTN ≥ 9 months (27.8 months) to those with a TTN < 9 months (13.5 months) (log-rank P < 0.001).
    Conclusions: TTN and nPSA are valuable predictors of prognosis in prostate cancer patients after ADT treatment, with better outcomes evident in patients with nPSA < 0.2 ng/mL and TTN > 9 months.
    Language English
    Publishing date 2023-02-09
    Publishing country Switzerland
    Document type Systematic Review
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2023.1047388
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Speed-Related Energy Flow and Joint Function Change During Human Walking.

    Hu, Zheqi / Ren, Lei / Hu, Dan / Gao, Yilei / Wei, Guowu / Qian, Zhihui / Wang, Kunyang

    Frontiers in bioengineering and biotechnology

    2021  Volume 9, Page(s) 666428

    Abstract: During human walking, mechanical energy transfers between ... ...

    Abstract During human walking, mechanical energy transfers between segments
    Language English
    Publishing date 2021-05-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2021.666428
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Cobalt Phthalocyanine Supported on Mesoporous CeO

    Song, Yibo / Hu, Siyuan / Cai, Dongren / Xiao, Jingran / Zhou, Shu-Feng / Zhan, Guowu

    ACS applied materials & interfaces

    2022  Volume 14, Issue 7, Page(s) 9151–9160

    Abstract: Heterogenization of biomolecules by immobilizing on a metal oxide support could greatly enhance their catalytic activity and stability, but their interactions are generally weak. Herein, cobalt phthalocyanine (CoPc) molecules were firmly anchored on a Ce- ...

    Abstract Heterogenization of biomolecules by immobilizing on a metal oxide support could greatly enhance their catalytic activity and stability, but their interactions are generally weak. Herein, cobalt phthalocyanine (CoPc) molecules were firmly anchored on a Ce-based metal-organic framework (Ce-BTC) due to π-π stacking interaction between CoPc and aromatic frameworks of the BTC linker, which was followed by a calcination treatment to convert Ce-BTC to mesoporous CeO
    Language English
    Publishing date 2022-02-08
    Publishing country United States
    Document type Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.1c23582
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Preparation of Polyclonal Antibody against ZmBT1 Protein and Its Application in Hormone-Regulated Starch Synthesis

    Liu, Lun / Qing, Yun / Shoaib, Noman / Di, Runze / Liu, Hanmei / Li, Yangping / Hu, Yufeng / Huang, Yubi / Yu, Guowu

    Agronomy. 2023 July 07, v. 13, no. 7

    2023  

    Abstract: In order to investigate the crucial role of ZmBT1 in starch accumulation during maize grain development and analyze the expression and distribution of ZmBT1 in various maize tissues, we prepared a polyclonal antibody. Specifically, we successfully ... ...

    Abstract In order to investigate the crucial role of ZmBT1 in starch accumulation during maize grain development and analyze the expression and distribution of ZmBT1 in various maize tissues, we prepared a polyclonal antibody. Specifically, we successfully expressed the recombinant plasmid pGEX-6p-ZmBT1-C (382-437aa) and purified Gst-ZmBT1-C as the antigen for antibody preparation. Our results confirmed that the ZmBT1 protein in maize tissues can be specifically recognized by the ZmBT1 antibody. Through Western blotting, we observed that the expression protein of ZmBT1 varied by tissues, with the highest content in the grain and endosperm. Furthermore, we employed a combination of Western blotting and quantitative real-time PCR to show that the expression level of ZmBT1 can be influenced by plant hormones. This finding suggests that ZmBT1 plays a critical role in the accumulation of starch and opens up new avenues for functional studies of this protein.
    Keywords agronomy ; antigens ; corn ; endosperm ; plasmids ; polyclonal antibodies ; quantitative polymerase chain reaction ; seed development ; starch
    Language English
    Dates of publication 2023-0707
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2607043-1
    ISSN 2073-4395
    ISSN 2073-4395
    DOI 10.3390/agronomy13071805
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Advances in the Current Understanding of the Mechanisms Governing the Acquisition of Castration-Resistant Prostate Cancer.

    Mao, Yifeng / Yang, Gaowei / Li, Yingbang / Liang, Guowu / Xu, Wangwang / Hu, Mingqiu

    Cancers

    2022  Volume 14, Issue 15

    Abstract: Despite aggressive treatment and androgen-deprivation therapy, most prostate cancer patients ultimately develop castration-resistant prostate cancer (CRPC), which is associated with high mortality rates. However, the mechanisms governing the development ... ...

    Abstract Despite aggressive treatment and androgen-deprivation therapy, most prostate cancer patients ultimately develop castration-resistant prostate cancer (CRPC), which is associated with high mortality rates. However, the mechanisms governing the development of CRPC are poorly understood, and androgen receptor (AR) signaling has been shown to be important in CRPC through AR gene mutations, gene overexpression, co-regulatory factors, AR shear variants, and androgen resynthesis. A growing number of non-AR pathways have also been shown to influence the CRPC progression, including the Wnt and Hh pathways. Moreover, non-coding RNAs have been identified as important regulators of the CRPC pathogenesis. The present review provides an overview of the relevant literature pertaining to the mechanisms governing the molecular acquisition of castration resistance in prostate cancer, providing a foundation for future, targeted therapeutic efforts.
    Language English
    Publishing date 2022-07-31
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers14153744
    Database MEDical Literature Analysis and Retrieval System OnLINE

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