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  1. Article ; Online: Introducing SELEX via a semester-long course-based undergraduate research experience (CURE).

    Kushner, David B

    Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology

    2021  Volume 49, Issue 4, Page(s) 605–618

    Abstract: With the growing importance of the field of RNA biology, undergraduates need to perform RNA-related research. Systematic evolution of ligands by exponential enrichment (SELEX) has become an important method in RNA biology. The principles of SELEX were ... ...

    Abstract With the growing importance of the field of RNA biology, undergraduates need to perform RNA-related research. Systematic evolution of ligands by exponential enrichment (SELEX) has become an important method in RNA biology. The principles of SELEX were applied to a semester-long course-based undergraduate research experience (CURE) in which two rounds of in vivo functional selection of regions of a viral RNA were performed. As the labwork had an unknown outcome, students indicated that they were excited by the work and became invested in the experience. By completing two rounds of SELEX, the students repeated molecular methods (e.g., RNA extraction, RT-PCR, agarose gel electrophoresis, DNA purification, cloning, and sequence analysis) and reported that repetition reinforced their learning and helped them build confidence in their lab abilities. Students also appreciated that they did not learn a "technique-per-week" without context, but rather they understood why certain methods were used for certain molecular tasks. Results from a 19-question multiple-choice assessment indicated increased comprehension of theory underlying methods performed. Details regarding experimental methods and timeline, and assessment and attitudinal results from three student cohorts, are described herein.
    MeSH term(s) Biomedical Research/education ; Brassica napus/virology ; Carmovirus/genetics ; Humans ; Molecular Biology/education ; Plant Diseases/genetics ; Plant Diseases/virology ; Plant Leaves/virology ; RNA, Viral/analysis ; RNA, Viral/genetics ; Research Design ; SELEX Aptamer Technique/methods ; Students/statistics & numerical data
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-04-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2039717-3
    ISSN 1539-3429 ; 1470-8175
    ISSN (online) 1539-3429
    ISSN 1470-8175
    DOI 10.1002/bmb.21519
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  2. Article ; Online: Virology in the Classroom: Current Approaches and Challenges to Undergraduate- and Graduate-Level Virology Education.

    Kushner, David B / Pekosz, Andrew

    Annual review of virology

    2021  Volume 8, Issue 1, Page(s) 537–558

    Abstract: The pervasive effects of the current coronavirus disease 2019 pandemic are but one reason for educators to refocus their efforts on virology teaching. Additionally, it is critical to understand how viruses function and to elucidate the relationship ... ...

    Abstract The pervasive effects of the current coronavirus disease 2019 pandemic are but one reason for educators to refocus their efforts on virology teaching. Additionally, it is critical to understand how viruses function and to elucidate the relationship between virus and host. An understanding of current virology education may improve pedagogical approaches for educating our students and trainees. Faculty who teach undergraduate microbiology indicate that approximately 10% of the course content features viruses; stand-alone virology courses are infrequently offered to undergraduates. Fortunately, virology taught to undergraduates includes foundational material; several approaches for delivery of lecture- and lab-based content exist. At the graduate education level, there is growing appreciation that an emphasis on logic, reasoning, inference, and statistics must be reintroduced into the curriculum to create a generation of scientists who have a greater capacity for creativity and innovation. Educators also need to remove barriers to student success, at all levels of education.
    MeSH term(s) COVID-19 ; Curriculum ; Education, Graduate ; Guidelines as Topic ; Humans ; Students ; Teaching ; Universities ; Virology/education
    Language English
    Publishing date 2021-07-09
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2764224-0
    ISSN 2327-0578 ; 2327-056X
    ISSN (online) 2327-0578
    ISSN 2327-056X
    DOI 10.1146/annurev-virology-091919-080047
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  3. Article ; Online: Vaccine Associated Measles Complicated by Suspected Measles Inclusion Body Encephalitis in a Pediatric Leukemia Patient and Stem Cell Transplant Recipient: A Focus on Clinical Evolution and Management.

    Kushner, Lauren E / Kamens, Jennifer / Bertaina, Alice / Shyr, David / Gans, Hayley A

    The Pediatric infectious disease journal

    2024  

    Abstract: Background: Immunocompromised individuals are at increased risk for severe disease and complications from viral infections, highlighting the importance of vaccination. However, in extremely rare situations, vaccine associated viral infections can be ... ...

    Abstract Background: Immunocompromised individuals are at increased risk for severe disease and complications from viral infections, highlighting the importance of vaccination. However, in extremely rare situations, vaccine associated viral infections can be associated with disseminated disease and complications in immunocompromised hosts.
    Case: Herein, we present a case of a 1-year-old child diagnosed with acute myeloid leukemia less than 2 weeks after receiving live viral vaccines who developed acute vaccine-strain measles virus disease, later complicated by central nervous system involvement following hematopoietic stem cell transplantation. A brain biopsy specimen was positive for vaccine-strain measles virus detected by reverse transcriptase polymerase chain reaction.
    Management and outcome: She was treated with intravenous ribavirin, inosine pranobex, intrathecal interferon-alpha and donor lymphocyte infusion following measles-mumps-rubella vaccine boost. Despite these measures, the patient suffered neurologic decline and dysautonomia, expiring after compassionate extubation. Management and ideal risk mitigation strategies are discussed within the context of existing literature for this rare complication.
    Language English
    Publishing date 2024-02-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 392481-6
    ISSN 1532-0987 ; 0891-3668
    ISSN (online) 1532-0987
    ISSN 0891-3668
    DOI 10.1097/INF.0000000000004299
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    Zs.A 1852: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Stroke Inpatient Rehabilitation Team Conferences: Leadership and Structure Improve Patient Outcomes.

    Kushner, David S / Strasser, Dale C

    Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association

    2020  Volume 29, Issue 4, Page(s) 104622

    Abstract: Consensus on how rehabilitation teamwork and services are optimally coordinated continues to be a work in progress. One area of recent research has been inpatient-rehabilitation team conferences in stroke. The prevalence of Americans living with stroke ... ...

    Abstract Consensus on how rehabilitation teamwork and services are optimally coordinated continues to be a work in progress. One area of recent research has been inpatient-rehabilitation team conferences in stroke. The prevalence of Americans living with stroke is expected to gradually increase as the U.S. population ages, as will the related direct and indirect costs. Effective interdisciplinary team conferences during acute-stroke inpatient-rehabilitation are key to managing long-term costs while improving functional outcomes. Effective team conferences help to identify patients at risk for medical complications and institutionalization and help to determine interventions that will focus on patients' medical, physical, cognitive, emotional, and social barriers to recovery and barriers to a community/home disposition. This scoping review paper identifies and analyzes literature on theory and structure of effective teams with the focus on stroke interdisciplinary rehabilitation team conferences and offers suggestions for improvement. Potential flaws of commonly used team conference formats are described. Studies are outlined showing associations between stroke patient outcomes and better care coordination and leadership in medical teamwork; and 2 examples of successful interdisciplinary team conference models used in stroke inpatient-rehabilitation are provided that support a case for a proactive, conscious structure to team conferences. Given the complexity of many stroke patients' clinical care, greater attention to team functioning, and especially team conference leadership and structure, may be a promising area of focus to improve the quality of health care services for people with stroke.
    MeSH term(s) Attitude of Health Personnel ; Cooperative Behavior ; Health Knowledge, Attitudes, Practice ; Humans ; Inpatients ; Interdisciplinary Communication ; Leadership ; Patient Care Planning/organization & administration ; Patient Care Team/organization & administration ; Quality Improvement/organization & administration ; Quality Indicators, Health Care/organization & administration ; Recovery of Function ; Stroke/diagnosis ; Stroke/physiopathology ; Stroke/therapy ; Stroke Rehabilitation ; Treatment Outcome
    Language English
    Publishing date 2020-02-06
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1131675-5
    ISSN 1532-8511 ; 1052-3057
    ISSN (online) 1532-8511
    ISSN 1052-3057
    DOI 10.1016/j.jstrokecerebrovasdis.2019.104622
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    Zs.A 3519: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  5. Article ; Online: 2016 ACR Presidential Address: Things Change: Patient- and Family-Centered Care.

    Kushner, David C

    Journal of the American College of Radiology : JACR

    2016  Volume 13, Issue 12 Pt A, Page(s) 1410–1414

    MeSH term(s) Delivery of Health Care/trends ; Family Health/trends ; Health Literacy/trends ; Informed Consent ; Patient-Centered Care/trends ; Physician-Patient Relations ; Radiology/trends ; United States
    Language English
    Publishing date 2016-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2274861-1
    ISSN 1558-349X ; 1546-1440
    ISSN (online) 1558-349X
    ISSN 1546-1440
    DOI 10.1016/j.jacr.2016.06.013
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    Zs.A 6636: Show issues Location:
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  6. Article ; Online: DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.

    Wolfson, Rachel L / Abdelaziz, Amira / Rankin, Genelle / Kushner, Sarah / Qi, Lijun / Mazor, Ofer / Choi, Seungwon / Sharma, Nikhil / Ginty, David D

    Cell

    2024  Volume 186, Issue 16, Page(s) 3368–3385.e18

    Abstract: The properties of dorsal root ganglia (DRG) neurons that innervate the distal colon are poorly defined, hindering our understanding of their roles in normal physiology and gastrointestinal (GI) disease. Here, we report genetically defined subsets of ... ...

    Abstract The properties of dorsal root ganglia (DRG) neurons that innervate the distal colon are poorly defined, hindering our understanding of their roles in normal physiology and gastrointestinal (GI) disease. Here, we report genetically defined subsets of colon-innervating DRG neurons with diverse morphologic and physiologic properties. Four colon-innervating DRG neuron populations are mechanosensitive and exhibit distinct force thresholds to colon distension. The highest threshold population, selectively labeled using Bmpr1b genetic tools, is necessary and sufficient for behavioral responses to high colon distension, which is partly mediated by the mechanosensory ion channel Piezo2. This Aδ-HTMR population mediates behavioral over-reactivity to colon distension caused by inflammation in a model of inflammatory bowel disease. Thus, like cutaneous DRG mechanoreceptor populations, colon-innervating mechanoreceptors exhibit distinct anatomical and physiological properties and tile force threshold space, and genetically defined colon-innervating HTMRs mediate pathophysiological responses to colon distension, revealing a target population for therapeutic intervention.
    MeSH term(s) Ganglia, Spinal ; Mechanoreceptors/physiology ; Colon ; Neurons ; Skin/innervation
    Language English
    Publishing date 2024-01-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2023.07.007
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    Zs.A 1167: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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    Zs.MG 58: Show issues

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  7. Article ; Online: Fluorescein Mapping in Vulvar Paget Disease.

    Wagar, Matthew K / Zhang, Ran Catherine / Weisman, Paul / Spencer, Ryan J / Kushner, David M

    Obstetrics and gynecology

    2023  Volume 141, Issue 3, Page(s) 608–612

    Abstract: Background: Vulvar Paget disease often requires extensive and, in some cases, multiple resections to treat. A fluorescein-mapping procedure followed by a staged vulvectomy may be an effective technique to tailor resection and identify clinically occult ... ...

    Abstract Background: Vulvar Paget disease often requires extensive and, in some cases, multiple resections to treat. A fluorescein-mapping procedure followed by a staged vulvectomy may be an effective technique to tailor resection and identify clinically occult lesions.
    Technique: We describe a two-step procedure; first, intravenous fluorescein sodium is injected, and the vulva is illuminated with a Wood's lamp. Representative biopsies are obtained and correlated on final pathology with the extent of disease to develop a final plan for excision. Second, using fluorescein to identify the confirmed areas of disease, the appropriate excisional procedure is performed once mapping biopsy pathology is known.
    Experience: We describe our experience with eight patients with vulvar Paget disease undergoing fluorescein mapping biopsies and staged vulvectomy. Using intravenous fluorescein sodium, all patients were found to have Paget disease beyond the visible margins of their gross lesions. No patients experienced a recurrence of Paget disease within a median follow-up time of 32 months, comparable with other directed methods of surgical resection.
    Conclusion: We report a technique for the injection of fluorescein sodium for the visualization of vulvar Paget disease capable of providing accurate surgical margins and identification of occult satellite lesions with a high degree of safety and a favorable cost profile. This staged approach to vulvectomy could offer improved accuracy of resection for vulvar Paget disease with few drawbacks.
    MeSH term(s) Female ; Humans ; Fluorescein ; Vulvar Neoplasms/diagnosis ; Vulvar Neoplasms/surgery ; Vulva/surgery ; Vulva/pathology ; Paget Disease, Extramammary/diagnosis ; Paget Disease, Extramammary/surgery ; Paget Disease, Extramammary/pathology ; Biopsy ; Neoplasm Recurrence, Local/pathology
    Chemical Substances Fluorescein (TPY09G7XIR)
    Language English
    Publishing date 2023-02-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 207330-4
    ISSN 1873-233X ; 0029-7844
    ISSN (online) 1873-233X
    ISSN 0029-7844
    DOI 10.1097/AOG.0000000000005084
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    Um I Zs.230: Show issues Location:
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  8. Article ; Online: Patient reported improvement in sexual health outcomes following care in a sexual health clinic for women with cancer.

    Rash, Joanne K / Seaborne, Lori A / Peterson, Megan / Kushner, David M / Sobecki, Janelle N

    Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer

    2023  Volume 31, Issue 3, Page(s) 171

    Abstract: Purpose: Sexual health concerns are common among female cancer survivors. Few data exist regarding patient-reported outcomes following interventions in this population. We aimed to determine patient-reported adherence and impact of interventions ... ...

    Abstract Purpose: Sexual health concerns are common among female cancer survivors. Few data exist regarding patient-reported outcomes following interventions in this population. We aimed to determine patient-reported adherence and impact of interventions provided in an academic specialty clinic for treatment of sexual health problems.
    Methods: A cross-sectional quality improvement survey regarding sexual problems, adherence with recommended therapies, and improvement following intervention was administered to all women seen at the Women's Integrative Sexual Health (WISH) program at the University of Wisconsin-Madison between November 2013 and July 2019. Descriptive and Kruskal-Wallis tests were used to explore differences between groups.
    Results: Two hundred twenty women (median age at first visit = 50 years, 53.1% breast cancer) were identified; N =113 surveys were completed (response rate = 49.6%). The most common presenting complaints were pain with intercourse (87.2%), vaginal dryness (85.3%), and low libido (82.6%). Menopausal women were more likely than premenopausal women to present with vaginal dryness (93.4% vs. 69.7%, p = .001) and pain with intercourse (93.4% vs. 76.5%, p = .02). Nearly all women adhered to recommendations for vaginal moisturizers/lubricants (96.9-100%) and vibrating vaginal wands (82.4-92.3%). A majority found recommended interventions helpful regardless of menopausal status or cancer type and reported persistent improvement. Nearly all women had improvement in understanding sexual health (92%) and would recommend the WISH program to others (91%).
    Conclusion: Women with cancer report integrative sexual health care to address sexual problems that are helpful and result in long-term improvement. Patients are overall highly adherent to recommended therapies, and nearly all would recommend the program to others.
    Implications for cancer survivors: Dedicated care to address sexual health in women after cancer treatment improves patient-reported sexual health outcomes across all cancer types.
    MeSH term(s) Female ; Humans ; Middle Aged ; Sexual Health ; Cross-Sectional Studies ; Breast Neoplasms/therapy ; Breast Neoplasms/epidemiology ; Surveys and Questionnaires ; Pain ; Patient Reported Outcome Measures ; Outcome Assessment, Health Care ; Sexual Behavior
    Language English
    Publishing date 2023-02-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1134446-5
    ISSN 1433-7339 ; 0941-4355
    ISSN (online) 1433-7339
    ISSN 0941-4355
    DOI 10.1007/s00520-023-07635-4
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    Zs.A 3697: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  9. Article: CURE on yeast genes of unknown function increases students' bioinformatics proficiency and research confidence.

    Miller, Mary / Tobin, Tammy / Aiello, David P / Hanson, Pamela / Strome, Erin / Johnston, Stephen D / Issel-Tarver, Laurie / Kushner, David B / Keeney, Jill B

    Journal of microbiology & biology education

    2024  Volume 25, Issue 1, Page(s) e0016523

    Abstract: Course-based undergraduate research experiences (CUREs) can reduce barriers to research opportunities while increasing student knowledge and confidence. However, the number of widely adopted, easily transferable CUREs is relatively small. Here, we ... ...

    Abstract Course-based undergraduate research experiences (CUREs) can reduce barriers to research opportunities while increasing student knowledge and confidence. However, the number of widely adopted, easily transferable CUREs is relatively small. Here, we describe a CURE aimed at determining the function of poorly characterized
    Language English
    Publishing date 2024-01-31
    Publishing country United States
    Document type Journal Article
    ISSN 1935-7877
    ISSN 1935-7877
    DOI 10.1128/jmbe.00165-23
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  10. Article: Strategies to avoid a missed diagnosis of co-occurring concussion in post-acute patients having a spinal cord injury.

    Kushner, David S

    Neural regeneration research

    2015  Volume 10, Issue 6, Page(s) 859–861

    Abstract: Research scientists and clinicians should be aware that missed diagnoses of mild-moderate traumatic brain injuries in post-acute patients having spinal cord injuries may approach 60-74% with certain risk factors, potentially causing clinical consequences ...

    Abstract Research scientists and clinicians should be aware that missed diagnoses of mild-moderate traumatic brain injuries in post-acute patients having spinal cord injuries may approach 60-74% with certain risk factors, potentially causing clinical consequences for patients, and confounding the results of clinical research studies. Factors leading to a missed diagnosis may include acute trauma-related life-threatening issues, sedation/intubation, subtle neuropathology on neuroimaging, failure to collect Glasgow Coma Scale scores or duration of posttraumatic amnesia, or lack of validity of this information, and overlap in neuro-cognitive symptoms with emotional responses to spinal cord injuries. Strategies for avoiding a missed diagnosis of mild-moderate traumatic brain injuries in patients having a spinal cord injuries are highlighted in this perspective.
    Language English
    Publishing date 2015-06-25
    Publishing country India
    Document type Journal Article
    ZDB-ID 2388460-5
    ISSN 1876-7958 ; 1673-5374
    ISSN (online) 1876-7958
    ISSN 1673-5374
    DOI 10.4103/1673-5374.158329
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