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Article ; Online: Behind the Scenes of the Human Breast Cell Atlas Project.

van Amerongen, Renée

Journal of mammary gland biology and neoplasia

2021 Volume 26, Issue 1, Page(s) 67–70

MeSH term(s)	Animals ; Biomedical Research/methods ; Biomedical Research/organization & administration ; Epithelial Cells/pathology ; Epithelial Cells/physiology ; Female ; Humans ; International Cooperation ; Mammary Glands, Animal/cytology ; Mammary Glands, Animal/pathology ; Mammary Glands, Animal/physiology ; Mammary Glands, Human/cytology ; Mammary Glands, Human/pathology ; Mammary Glands, Human/physiology ; Mice ; Sequence Analysis, RNA/methods ; Single-Cell Analysis/methods ; Spatial Analysis ; Transcriptome
Language	English
Publishing date	2021-04-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1327345-0
ISSN	1573-7039 ; 1083-3021
ISSN (online)	1573-7039
ISSN	1083-3021
DOI	10.1007/s10911-021-09482-7
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Article ; Online: Visualizing WNT signaling in mammalian systems.

van der Wal, Tanne / van Amerongen, Renée

Current topics in developmental biology

2023 Volume 153, Page(s) 61–93

Abstract: WNT/CTNNB1 signaling plays a critical role in the development of all multicellular animals. Here, we include both the embryonic stages, during which tissue morphogenesis takes place, and the postnatal stages of development, during which tissue ... ...

Abstract	WNT/CTNNB1 signaling plays a critical role in the development of all multicellular animals. Here, we include both the embryonic stages, during which tissue morphogenesis takes place, and the postnatal stages of development, during which tissue homeostasis occurs. Thus, embryonic development concerns lineage development and cell fate specification, while postnatal development involves tissue maintenance and regeneration. Multiple tools are available to researchers who want to investigate, and ideally visualize, the dynamic and pleiotropic involvement of WNT/CTNNB1 signaling in these processes. Here, we discuss and evaluate the decisions that researchers need to make in identifying the experimental system and appropriate tools for the specific question they want to address, covering different types of WNT/CTNNB1 reporters in cells and mice. At a molecular level, advanced quantitative imaging techniques can provide spatio-temporal information that cannot be provided by traditional biochemical assays. We therefore also highlight some recent studies to show their potential in deciphering the complex and dynamic mechanisms that drive WNT/CTNNB1 signaling.
MeSH term(s)	Animals ; Mice ; Wnt Signaling Pathway ; beta Catenin/metabolism ; Cell Differentiation ; Mammals/metabolism
Chemical Substances	beta Catenin
Language	English
Publishing date	2023-03-16
Publishing country	United States
Document type	Journal Article
ISSN	1557-8933 ; 0070-2153
ISSN (online)	1557-8933
ISSN	0070-2153
DOI	10.1016/bs.ctdb.2023.02.001
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Article ; Online: Celebrating Discoveries in Wnt Signaling: How One Man Gave Wings to an Entire Field.

van Amerongen, Renée

Cell

2020 Volume 181, Issue 3, Page(s) 487–491

Abstract: This year's Gairdner Foundation Award for Biomedical Research goes to Roel Nusse for his pioneering work on the Wnt signaling pathway and its many roles in development, cancer, and stem cells. ...

Abstract	This year's Gairdner Foundation Award for Biomedical Research goes to Roel Nusse for his pioneering work on the Wnt signaling pathway and its many roles in development, cancer, and stem cells.
MeSH term(s)	Animals ; Animals, Genetically Modified/metabolism ; Bibliographies as Topic ; Cell Communication ; Drosophila ; Drosophila Proteins/metabolism ; Female ; Humans ; Mammary Neoplasms, Animal/metabolism ; Mammary Neoplasms, Animal/pathology ; Mice ; Wnt Proteins/metabolism ; Wnt Signaling Pathway ; Wnt1 Protein/metabolism ; beta Catenin/metabolism
Chemical Substances	Drosophila Proteins ; Wnt Proteins ; Wnt1 Protein ; beta Catenin ; wg protein, Drosophila
Language	English
Publishing date	2020-03-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2020.03.033
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Zs.A 1167: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 58: Show issues

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Article ; Online: Connecting the Dots: Mammary Gland and Breast Cancer at Single Cell Resolution.

van Amerongen, Renée / Kordon, Edith C / Koledova, Zuzana

Journal of mammary gland biology and neoplasia

2021 Volume 26, Issue 1, Page(s) 1–2

MeSH term(s)	Animals ; Breast Neoplasms/pathology ; Female ; Humans ; Mammary Glands, Animal/cytology ; Mammary Glands, Animal/pathology ; Mammary Glands, Animal/physiology ; Mammary Glands, Human/cytology ; Mammary Glands, Human/pathology ; Mammary Glands, Human/physiology ; Mammary Neoplasms, Animal/pathology ; Mice ; Microscopy/methods ; Microscopy/trends ; Single-Cell Analysis/methods ; Single-Cell Analysis/trends
Language	English
Publishing date	2021-06-14
Publishing country	United States
Document type	Editorial ; Introductory Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1327345-0
ISSN	1573-7039 ; 1083-3021
ISSN (online)	1573-7039
ISSN	1083-3021
DOI	10.1007/s10911-021-09492-5
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Zs.A 4478: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Zooming in on the WNT/CTNNB1 Destruction Complex: Functional Mechanistic Details with Implications for Therapeutic Targeting.

de Man, Saskia Madelon Ada / van Amerongen, Renée

Handbook of experimental pharmacology

2021 Volume 269, Page(s) 137–173

Abstract: WNT/CTNNB1 signaling is crucial for balancing cell proliferation and differentiation in all multicellular animals. CTNNB1 accumulation is the hallmark of WNT/CTNNB1 pathway activation and the key downstream event in both a physiological and an oncogenic ... ...

Abstract	WNT/CTNNB1 signaling is crucial for balancing cell proliferation and differentiation in all multicellular animals. CTNNB1 accumulation is the hallmark of WNT/CTNNB1 pathway activation and the key downstream event in both a physiological and an oncogenic context. In the absence of WNT stimulation, the cytoplasmic and nuclear levels of CTNNB1 are kept low because of its sequestration and phosphorylation by the so-called destruction complex, which targets CTNNB1 for proteasomal degradation. In the presence of WNT proteins, or as a result of oncogenic mutations, this process is impaired and CTNNB1 levels become elevated.Here we discuss recent advances in our understanding of destruction complex activity and inactivation, focusing on the individual components and interactions that ultimately control CTNNB1 turnover (in the "WNT off" situation) and stabilization (in the "WNT on" situation). We especially highlight the insights gleaned from recent quantitative, image-based studies, which paint an unprecedentedly detailed picture of the dynamic events that control destruction protein complex composition and function. We argue that these mechanistic details may reveal new opportunities for therapeutic intervention and could result in the destruction complex re-emerging as a target for therapy in cancer.
MeSH term(s)	Animals ; Cell Proliferation ; Phosphorylation ; Wnt Proteins/metabolism ; Wnt Signaling Pathway ; beta Catenin/metabolism
Chemical Substances	Wnt Proteins ; beta Catenin
Language	English
Publishing date	2021-09-06
Publishing country	Germany
Document type	Journal Article
ISSN	0171-2004
ISSN	0171-2004
DOI	10.1007/164_2021_522
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Article: Aberrant WNT/CTNNB1 Signaling as a Therapeutic Target in Human Breast Cancer: Weighing the Evidence.

van Schie, Emma H / van Amerongen, Renée

Frontiers in cell and developmental biology

2020 Volume 8, Page(s) 25

Abstract: WNT signaling is crucial for tissue morphogenesis during development in all multicellular animals. After birth, WNT/CTNNB1 responsive stem cells are responsible for tissue homeostasis in various organs and hyperactive WNT/CTNNB1 signaling is observed in ... ...

Abstract	WNT signaling is crucial for tissue morphogenesis during development in all multicellular animals. After birth, WNT/CTNNB1 responsive stem cells are responsible for tissue homeostasis in various organs and hyperactive WNT/CTNNB1 signaling is observed in many different human cancers. The first link between WNT signaling and breast cancer was established almost 40 years ago, when
Language	English
Publishing date	2020-01-31
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2020.00025
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Article ; Online: A molecular toolbox to study progesterone receptor signaling.

Aarts, Marleen T / Wagner, Muriel / van der Wal, Tanne / van Boxtel, Antonius L / van Amerongen, Renée

Journal of mammary gland biology and neoplasia

2023 Volume 28, Issue 1, Page(s) 24

Abstract: Progesterone receptor (PR) signaling is required for mammary gland development and homeostasis. A major bottleneck in studying PR signaling is the lack of sensitive assays to measure and visualize PR pathway activity both quantitatively and spatially. ... ...

Abstract	Progesterone receptor (PR) signaling is required for mammary gland development and homeostasis. A major bottleneck in studying PR signaling is the lack of sensitive assays to measure and visualize PR pathway activity both quantitatively and spatially. Here, we develop new tools to study PR signaling in human breast epithelial cells. First, we generate optimized Progesterone Responsive Element (PRE)-luciferase constructs and demonstrate that these new reporters are a powerful tool to quantify PR signaling activity across a wide range of progesterone concentrations in two luminal breast cancer cell lines, MCF7 and T47D. We also describe a fluorescent lentiviral PRE-GFP reporter as a novel tool to visualize PR signaling at the single-cell level. Our reporter constructs are sensitive to physiological levels of progesterone. Second, we show that low background signaling, and high levels of PR expression are a prerequisite for robustly measuring PR signaling. Increasing PR expression by transient transfection, stable overexpression in MCF7 or clonal selection in T47D, drastically improves both the dynamic range of luciferase reporter assays, and the induction of endogenous PR target genes as measured by qRT-PCR. We find that the PR signaling response differs per cell line, target gene and hormone concentration used. Taken together, our tools allow a more rationally designed approach for measuring PR signaling in breast epithelial cells.
MeSH term(s)	Humans ; Progesterone ; Receptors, Progesterone ; Signal Transduction ; MCF-7 Cells ; Luciferases
Chemical Substances	Progesterone (4G7DS2Q64Y) ; Receptors, Progesterone ; Luciferases (EC 1.13.12.-)
Language	English
Publishing date	2023-11-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1327345-0
ISSN	1573-7039 ; 1083-3021
ISSN (online)	1573-7039
ISSN	1083-3021
DOI	10.1007/s10911-023-09550-0
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Zs.A 4478: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: How to Use Online Tools to Generate New Hypotheses for Mammary Gland Biology Research: A Case Study for Wnt7b.

van de Grift, Yorick Bernardus Cornelis / Heijmans, Nika / van Amerongen, Renée

Journal of mammary gland biology and neoplasia

2021 Volume 25, Issue 4, Page(s) 319–335

Abstract: An increasing number of '-omics' datasets, generated by labs all across the world, are becoming available. They contain a wealth of data that are largely unexplored. Not every scientist, however, will have access to the required resources and expertise ... ...

Abstract	An increasing number of '-omics' datasets, generated by labs all across the world, are becoming available. They contain a wealth of data that are largely unexplored. Not every scientist, however, will have access to the required resources and expertise to analyze such data from scratch. Fortunately, a growing number of investigators is dedicating their time and effort to the development of user friendly, online applications that allow researchers to use and investigate these datasets. Here, we will illustrate the usefulness of such an approach. Using regulation of Wnt7b expression as an example, we will highlight a selection of accessible tools and resources that are available to researchers in the area of mammary gland biology. We show how they can be used for in silico analyses of gene regulatory mechanisms, resulting in new hypotheses and providing leads for experimental follow up. We also call out to the mammary gland community to join forces in a coordinated effort to generate and share additional tissue-specific '-omics' datasets and thereby expand the in silico toolbox.
MeSH term(s)	Animals ; Breast Neoplasms/genetics ; Breast Neoplasms/pathology ; Computational Biology/methods ; Databases, Genetic ; Datasets as Topic ; Feasibility Studies ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Internet ; Mammary Glands, Human/growth & development ; Mammary Glands, Human/pathology ; Mice ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins/metabolism ; RNA-Seq ; Single-Cell Analysis ; Spatio-Temporal Analysis ; Wnt Proteins/genetics ; Wnt Proteins/metabolism ; Wnt Signaling Pathway/genetics
Chemical Substances	Proto-Oncogene Proteins ; WNT7B protein, human ; Wnt Proteins ; Wnt7b protein, mouse
Language	English
Publishing date	2021-02-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1327345-0
ISSN	1573-7039 ; 1083-3021
ISSN (online)	1573-7039
ISSN	1083-3021
DOI	10.1007/s10911-020-09474-z
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Zs.A 4478: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Walking the tight wire between cell adhesion and WNT signalling: a balancing act for β-catenin.

van der Wal, Tanne / van Amerongen, Renée

Open biology

2020 Volume 10, Issue 12, Page(s) 200267

Abstract: CTNNB1 (catenin β-1, also known as β-catenin) plays a dual role in the cell. It is the key effector of WNT/CTNNB1 signalling, acting as a transcriptional co-activator of TCF/LEF target genes. It is also crucial for cell adhesion and a critical component ... ...

Abstract	CTNNB1 (catenin β-1, also known as β-catenin) plays a dual role in the cell. It is the key effector of WNT/CTNNB1 signalling, acting as a transcriptional co-activator of TCF/LEF target genes. It is also crucial for cell adhesion and a critical component of cadherin-based adherens junctions. Two functional pools of CTNNB1, a transcriptionally active and an adhesive pool, can therefore be distinguished. Whether cells merely balance the distribution of available CTNNB1 between these functional pools or whether interplay occurs between them has long been studied and debated. While interplay has been indicated upon artificial modulation of cadherin expression levels and during epithelial-mesenchymal transition, it is unclear to what extent CTNNB1 exchange occurs under physiological conditions and in response to WNT stimulation. Here, we review the available evidence for both of these models, discuss how CTNNB1 binding to its many interaction partners is controlled and propose avenues for future studies.
MeSH term(s)	Animals ; Biomarkers ; Cadherins/genetics ; Cadherins/metabolism ; Cell Adhesion/genetics ; Epithelial-Mesenchymal Transition/genetics ; Gene Expression Regulation ; Humans ; Protein Transport ; Wnt Signaling Pathway ; beta Catenin/genetics ; beta Catenin/metabolism
Chemical Substances	Biomarkers ; Cadherins ; beta Catenin
Language	English
Publishing date	2020-12-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2630944-0
ISSN	2046-2441 ; 2046-2441
ISSN (online)	2046-2441
ISSN	2046-2441
DOI	10.1098/rsob.200267
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Article ; Online: Lineage Tracing in the Mammary Gland Using Cre/lox Technology and Fluorescent Reporter Alleles.

van Amerongen, Renée

Methods in molecular biology (Clifton, N.J.)

2015 Volume 1293, Page(s) 187–211

Abstract: Lineage tracing using Cre/lox technology has become a well-established technique to study the contribution of different (stem) cell populations to organ development and function. When used in the mammary gland, it forms a valuable addition to the already ...

Abstract	Lineage tracing using Cre/lox technology has become a well-established technique to study the contribution of different (stem) cell populations to organ development and function. When used in the mammary gland, it forms a valuable addition to the already existing experimental toolbox and an important alternative to other readouts measuring stem cell potential, such as the fat pad transplantation assay.Here I describe how to set up and analyze an in vivo lineage tracing experiment using tamoxifen-inducible Cre/lox technology, highlighting the specific challenges that the investigator faces when employing this method and interpreting the results in the mammary gland.
MeSH term(s)	Alleles ; Animals ; Cell Lineage/genetics ; Cell Tracking/methods ; Female ; Gene Expression ; Genes, Reporter ; Homologous Recombination ; Mammary Glands, Animal/cytology ; Mice ; Microscopy, Confocal ; Stem Cells/cytology ; Stem Cells/metabolism ; Tamoxifen
Chemical Substances	Tamoxifen (094ZI81Y45)
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-2519-3_11
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