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Book: Interstitium, connective tissue and lymphatics

Reed, Rolf K.

Glasgow, UK, [1993]

(Proceedings of the ... congress of the International Union of the Physiological Sciences ; 32 ; Portland Press proceedings ; 9)

1995

Author's details	ed. R. K. Reed
Series title	Proceedings of the ... congress of the International Union of the Physiological Sciences ; 32 Portland Press proceedings ; 9 Proceedings of the ... congress of the International Union of Physiological Sciences (IUPS)
Collection	Proceedings of the ... congress of the International Union of Physiological Sciences (IUPS)
Keywords	Extracellular Matrix / physiology / congresses ; Connective Tissue / physiology / congresses ; Lymphatic System / physiology / congresses
Language	English
Size	XIV, 341 S. : Ill., graph. Darst.
Publisher	Portland
Publishing place	London u.a.
Publishing country	Great Britain
Document type	Book
HBZ-ID	HT007243462
ISBN	1-85578-073-9 ; 978-1-85578-073-6
Database	Catalogue ZB MED Medicine, Health

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Book: AUTOREGULATION OF INTERSTITIAL FLUID VOLUME IN RAT SKELETAL MUSCLE

Reed, Rolf K.

1982

Size	GETR. ZAEHLUNG
Document type	Book
Note	BERGEN, UNIV., DISS., 1982
HBZ-ID	HT002724994
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Article ; Online: Protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative breast cancer xenograft model.

Tveitarås, Maria K / Selheim, Frode / Sortland, Kristina / Reed, Rolf K / Stuhr, Linda

PloS one

2019 Volume 14, Issue 5, Page(s) e0215909

Abstract: The main objective of this study was to identify single proteins or protein networks that might be used as diagnostic biomarkers or for therapeutic purposes by evaluating the protein expression profiling of plasma and lungs at different stages of ... ...

Abstract	The main objective of this study was to identify single proteins or protein networks that might be used as diagnostic biomarkers or for therapeutic purposes by evaluating the protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative MDA-MB-231 breast cancer xenograft model. MDA-MB-231 tumour cells were injected into the mammary fat pads on one side of the groin area. The mice were sacrificed day 19 (pre-metastases) and day 54 (metastases). Non-injected mice served as controls. Plasma was collected and lungs harvested for both immunohistochemistry and protein analysis. The most striking observation in plasma was the initial reduction in haptoglobin level at the pre-metastatic stage, to a following significant increase in haptoglobin level at the metastatic stage, with a more than 4000-fold increase from the pre-metastatic to the metastatic phase. A corresponding increase in haptoglobin level was also found in lung tissue after metastasis. Fibrinogen beta chain also had a similar change in expression level in plasma as haptoglobin, however not as prominent. There were also changes in plasma thrombospondin-4 and transferrin receptor protein 1 levels, from an increase at the pre-metastatic stage, to a significant fall when metastases were established. This suggests that especially changes in haptoglobin, but also fibrinogen beta chain, thrombospondin-4 and transferrin receptor protein 1 is indicative of metastasis, at least in this breast cancer model, and should be further evaluated as general breast cancer biomarkers.
MeSH term(s)	Animals ; Biomarkers, Tumor/blood ; Blood Proteins/analysis ; Blood Proteins/metabolism ; Cell Line, Tumor ; Female ; Humans ; Lung/metabolism ; Lung/pathology ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Lung Neoplasms/secondary ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Neoplasm Metastasis ; Neoplasm Staging ; Proteomics/methods ; Transplantation, Heterologous ; Triple Negative Breast Neoplasms/pathology
Chemical Substances	Biomarkers, Tumor ; Blood Proteins
Language	English
Publishing date	2019-05-01
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0215909
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Integrin α

Lidén, Åsa / Karlsen, Tine Veronika / Guss, Bengt / Reed, Rolf K / Rubin, Kristofer

Experimental physiology

2018 Volume 103, Issue 5, Page(s) 629–634

Abstract: New findings: What is the central question of this study? Collagen-binding β: Abstract: Accumulated data indicate that cell-mediated contraction of reconstituted collagenous gels in vitro can serve as a model for cell-mediated control of interstitial ...

Abstract	New findings: What is the central question of this study? Collagen-binding β Abstract: Accumulated data indicate that cell-mediated contraction of reconstituted collagenous gels in vitro can serve as a model for cell-mediated control of interstitial fluid pressure (P
MeSH term(s)	Animals ; Collagen/metabolism ; Edema/metabolism ; Extracellular Fluid/metabolism ; Extracellular Matrix/metabolism ; Fibronectins/metabolism ; Integrin alphaVbeta3/metabolism ; Integrin beta1/metabolism ; Mast Cells/metabolism ; Mice ; Mice, Inbred C57BL ; Platelet-Derived Growth Factor/metabolism ; Pressure
Chemical Substances	Fibronectins ; Integrin alphaVbeta3 ; Integrin beta1 ; Platelet-Derived Growth Factor ; Collagen (9007-34-5)
Language	English
Publishing date	2018-03-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1016295-1
ISSN	1469-445X ; 0958-0670
ISSN (online)	1469-445X
ISSN	0958-0670
DOI	10.1113/EP086902
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Article ; Online: Protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative breast cancer xenograft model.

Maria K Tveitarås / Frode Selheim / Kristina Sortland / Rolf K Reed / Linda Stuhr

PLoS ONE, Vol 14, Iss 5, p e

2019 Volume 0215909

Abstract	The main objective of this study was to identify single proteins or protein networks that might be used as diagnostic biomarkers or for therapeutic purposes by evaluating the protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative MDA-MB-231 breast cancer xenograft model. MDA-MB-231 tumour cells were injected into the mammary fat pads on one side of the groin area. The mice were sacrificed day 19 (pre-metastases) and day 54 (metastases). Non-injected mice served as controls. Plasma was collected and lungs harvested for both immunohistochemistry and protein analysis. The most striking observation in plasma was the initial reduction in haptoglobin level at the pre-metastatic stage, to a following significant increase in haptoglobin level at the metastatic stage, with a more than 4000-fold increase from the pre-metastatic to the metastatic phase. A corresponding increase in haptoglobin level was also found in lung tissue after metastasis. Fibrinogen beta chain also had a similar change in expression level in plasma as haptoglobin, however not as prominent. There were also changes in plasma thrombospondin-4 and transferrin receptor protein 1 levels, from an increase at the pre-metastatic stage, to a significant fall when metastases were established. This suggests that especially changes in haptoglobin, but also fibrinogen beta chain, thrombospondin-4 and transferrin receptor protein 1 is indicative of metastasis, at least in this breast cancer model, and should be further evaluated as general breast cancer biomarkers.
Keywords	Medicine ; R ; Science ; Q
Subject code	616
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Stromal integrin α11-deficiency reduces interstitial fluid pressure and perturbs collagen structure in triple-negative breast xenograft tumors.

Smeland, Hilde Ytre-Hauge / Lu, Ning / Karlsen, Tine V / Salvesen, Gerd / Reed, Rolf K / Stuhr, Linda

BMC cancer

2019 Volume 19, Issue 1, Page(s) 234

Abstract: Background: Cancer progression is influenced by a pro-tumorigenic microenvironment. The aberrant tumor stroma with increased collagen deposition, contractile fibroblasts and dysfunctional vessels has a major impact on the interstitial fluid pressure ( ... ...

Abstract	Background: Cancer progression is influenced by a pro-tumorigenic microenvironment. The aberrant tumor stroma with increased collagen deposition, contractile fibroblasts and dysfunctional vessels has a major impact on the interstitial fluid pressure (PIF) in most solid tumors. An increased tumor PIF is a barrier to the transport of interstitial fluid into and within the tumor. Therefore, understanding the mechanisms that regulate pressure homeostasis can lead to new insight into breast tumor progression, invasion and response to therapy. The collagen binding integrin α11β1 is upregulated during myofibroblast differentiation and expressed on fibroblasts in the tumor stroma. As a collagen organizer and a probable link between contractile fibroblasts and the complex collagen network in tumors, integrin α11β1 could be a potential regulator of tumor PIF. Methods: We investigated the effect of stromal integrin α11-deficiency on pressure homeostasis, collagen organization and tumor growth using orthotopic and ectopic triple-negative breast cancer xenografts (MDA-MB-231 and MDA-MB-468) in wild type and integrin α11-deficient mice. PIF was measured by the wick-in-needle technique, collagen by Picrosirius Red staining and electron microscopy, and uptake of radioactively labeled 5FU by microdialysis. Further, PIF in heterospheroids composed of MDA-MB-231 cells and wild type or integrin α11-deficient fibroblasts was measured by micropuncture. Results: Stromal integrin α11-deficiency decreased PIF in both the orthotopic breast cancer models. A concomitant perturbed collagen structure was seen, with fewer aligned and thinner fibrils. Integrin α11-deficiency also impeded MDA-MB-231 breast tumor growth, but no effect was observed on drug uptake. No effects were seen in the ectopic model. By investigating the isolated effect of integrin α11-positive fibroblasts on MDA-MB-231 cells in vitro, we provide evidence that PIF regulation was mediated by integrin α11-positive fibroblasts. Conclusion: We hereby show the importance of integrin α11β1 in pressure homeostasis in triple-negative breast tumors, indicating a new role for integrin α11β1 in the tumor microenvironment. Our data suggest that integrin α11β1 has a pro-tumorigenic effect on triple-negative breast cancer growth in vivo. The significance of the local microenvironment is shown by the different effects of integrin α11β1 in the orthotopic and ectopic models, underlining the importance of choosing an appropriate preclinical model.
MeSH term(s)	Animals ; Cell Line, Tumor ; Collagen/chemistry ; Cytoprotection ; Extracellular Fluid/metabolism ; Female ; Gene Knockout Techniques ; Humans ; Integrin alpha Chains/genetics ; Integrins/metabolism ; Mice ; Neoplasm Transplantation ; Receptors, Collagen/metabolism ; Stromal Cells ; Triple Negative Breast Neoplasms/chemistry ; Triple Negative Breast Neoplasms/genetics ; Triple Negative Breast Neoplasms/metabolism ; Tumor Microenvironment
Chemical Substances	Integrin alpha Chains ; Integrins ; Receptors, Collagen ; integrin alpha11, mouse ; integrin alpha11beta1 ; Collagen (9007-34-5)
Language	English
Publishing date	2019-03-15
Publishing country	England
Document type	Journal Article
ISSN	1471-2407
ISSN (online)	1471-2407
DOI	10.1186/s12885-019-5449-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Epac1 Is Crucial for Maintenance of Endothelial Barrier Function through A Mechanism Partly Independent of Rac1.

García-Ponce, Alexander / Schuster, Katharina / Døskeland, Stein-Ove / Reed, Rolf K / Curry, Fitz-Roy E / Waschke, Jens / Radeva, Mariya Y

Cells

2020 Volume 9, Issue 10

Abstract: Epac1 (exchange protein activated by cAMP) stabilizes the endothelial barrier, but detailed studies are limited by the side effects of pharmacological Epac1 modulators and transient transfections. Here, we compare the key properties of barriers between ... ...

Abstract	Epac1 (exchange protein activated by cAMP) stabilizes the endothelial barrier, but detailed studies are limited by the side effects of pharmacological Epac1 modulators and transient transfections. Here, we compare the key properties of barriers between endothelial cells derived from wild-type (WT) and Epac1-knockout (KO) mice myocardium. We found that KO cell layers, unlike WT layers, had low and cAMP-insensitive trans-endothelial resistance (TER). They also had fragmented VE-cadherin staining despite having augmented cAMP levels and increased protein expression of Rap1, Rac1, RhoA, and VE-cadherin. The simultaneous direct activation of Rac1 and RhoA by CN04 compensated Epac1 loss, since TER was increased. In KO-cells, inhibition of Rac1 activity had no additional effect on TER, suggesting that other mechanisms compensate the inhibition of the Rac1 function to preserve barrier properties. In summary, Epac1 is crucial for baseline and cAMP-mediated barrier stabilization through mechanisms that are at least partially independent of Rac1.
MeSH term(s)	Animals ; Antigens, CD/genetics ; Cadherins/genetics ; Cell Membrane Permeability/drug effects ; Cyclic AMP/genetics ; Endothelial Cells/metabolism ; Endothelial Cells/pathology ; Gene Expression Regulation/drug effects ; Guanine Nucleotide Exchange Factors/genetics ; Humans ; Mice ; Mice, Knockout ; Myocardium/metabolism ; Myocardium/pathology ; Neuropeptides/agonists ; Neuropeptides/genetics ; Signal Transduction/genetics ; Transcriptional Activation/drug effects ; rac1 GTP-Binding Protein/agonists ; rac1 GTP-Binding Protein/genetics ; rap1 GTP-Binding Proteins/drug effects ; rhoA GTP-Binding Protein/agonists ; rhoA GTP-Binding Protein/genetics
Chemical Substances	Antigens, CD ; Cadherins ; Epac protein, mouse ; Guanine Nucleotide Exchange Factors ; Neuropeptides ; Rac1 protein, mouse ; cadherin 5 ; Cyclic AMP (E0399OZS9N) ; Rap1 protein, mouse (EC 3.6.5.2) ; RhoA protein, mouse (EC 3.6.5.2) ; rac1 GTP-Binding Protein (EC 3.6.5.2) ; rap1 GTP-Binding Proteins (EC 3.6.5.2) ; rhoA GTP-Binding Protein (EC 3.6.5.2)
Language	English
Publishing date	2020-09-25
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells9102170
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Article ; Online: Hyperbaric oxygen treatment did not significantly affect radiation injury in the mandibular area of rats.

Sønstevold, Tonje / Johannessen, Anne Christine / Reed, Rolf K / Salvesen, Gerd S / Stuhr, Linda

Oral surgery, oral medicine, oral pathology and oral radiology

2018 Volume 125, Issue 2, Page(s) 112–119

Abstract: Objective: Hyperbaric oxygen therapy (HBOT) has been used to enhance microcirculation and thereby oxygen tension in tissues. The present study aimed to investigate the effect of HBOT on radiation injury in the mandibular area of rats.: Study design: ... ...

Abstract	Objective: Hyperbaric oxygen therapy (HBOT) has been used to enhance microcirculation and thereby oxygen tension in tissues. The present study aimed to investigate the effect of HBOT on radiation injury in the mandibular area of rats. Study design: The left mandibles of rats were irradiated by external radiotherapy (15 Gy every other week for a total of 75 Gy). Four HBOT strategies were used: 2 prophylactic groups receiving HBOT either between each radiation treatment or immediately following terminated radiation treatment, and 2 therapeutic groups receiving HBOT after the latent period of 6 weeks after irradiation either every day (standard HBOT protocol) or 3 days a week for 6 weeks. Tissue samples of the irradiated area were taken from skin, the salivary gland, and the mandible. All tissues were stained with hematoxylin and eosin for morphologic examination. Furthermore, skin samples were stained with CD31 for blood vessel analysis. Results: There was no change in blood vessel density or morphology between controls and HBOT tissues after radiation. The dentin of 2 of the 5 rats that received HBOT either normalized or was not affected by irradiation. Conclusions: HBOT did not affect radiation injury of the mandibular area in rats within 12 weeks after irradiation.
MeSH term(s)	Animals ; Disease Models, Animal ; Hyperbaric Oxygenation/methods ; Male ; Mandible/blood supply ; Mandible/radiation effects ; Microcirculation ; Radiation Dosage ; Radiation Injuries/therapy ; Random Allocation ; Rats ; Rats, Sprague-Dawley
Language	English
Publishing date	2018
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2650843-6
ISSN	2212-4411 ; 2212-4403
ISSN (online)	2212-4411
ISSN	2212-4403
DOI	10.1016/j.oooo.2017.10.014
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Article ; Online: Epac1 Is Crucial for Maintenance of Endothelial Barrier Function through A Mechanism Partly Independent of Rac1

Alexander García-Ponce / Katharina Schuster / Stein-Ove Døskeland / Rolf K. Reed / Fitz-Roy E. Curry / Jens Waschke / Mariya Y. Radeva

Cells, Vol 9, Iss 2170, p

2020 Volume 2170

Abstract	Epac1 (exchange protein activated by cAMP) stabilizes the endothelial barrier, but detailed studies are limited by the side effects of pharmacological Epac1 modulators and transient transfections. Here, we compare the key properties of barriers between endothelial cells derived from wild-type (WT) and Epac1-knockout (KO) mice myocardium. We found that KO cell layers, unlike WT layers, had low and cAMP-insensitive trans-endothelial resistance (TER). They also had fragmented VE-cadherin staining despite having augmented cAMP levels and increased protein expression of Rap1, Rac1, RhoA, and VE-cadherin. The simultaneous direct activation of Rac1 and RhoA by CN04 compensated Epac1 loss, since TER was increased. In KO-cells, inhibition of Rac1 activity had no additional effect on TER, suggesting that other mechanisms compensate the inhibition of the Rac1 function to preserve barrier properties. In summary, Epac1 is crucial for baseline and cAMP-mediated barrier stabilization through mechanisms that are at least partially independent of Rac1.
Keywords	cAMP ; endothelial barrier ; Epac1 ; Rho GTPases ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Gene expression patterns in Atlantic salmon (Salmo salar) with severe nephrocalcinosis.

Klykken, Christine / Boissonnot, Lauris / Reed, Anne Katrine / Whatmore, Paul / Attramadal, Kari / Olsen, Rolf Erik

Journal of fish diseases

2022 Volume 45, Issue 11, Page(s) 1645–1658

Abstract: Nephrocalcinosis is a common disorder in farmed Atlantic salmon, but the consequences for the fish physiology are not well understood. We performed a transcriptome study in kidneys of Atlantic salmon (Salmo salar) smolts without and with severe chronic ... ...

Abstract	Nephrocalcinosis is a common disorder in farmed Atlantic salmon, but the consequences for the fish physiology are not well understood. We performed a transcriptome study in kidneys of Atlantic salmon (Salmo salar) smolts without and with severe chronic nephrocalcinosis (NC). The study revealed that numerous genes are differentially expressed in fish with NC compared with healthy salmon. The most evident changes in gene expression patterns in the NC group were a massive downregulation of metabolism and energy production, upregulation of signalling pathways important for tissue repair and function maintenance and upregulation of inflammatory responses. Overall, the extensive tissue damage and the gene regulation responses that affect salmon with severe nephrocalcinosis are highly likely to have dramatic consequences on fish survival.
MeSH term(s)	Animals ; Fish Diseases/genetics ; Gene Expression Regulation ; Nephrocalcinosis/genetics ; Nephrocalcinosis/veterinary ; Salmo salar/genetics ; Transcriptome
Language	English
Publishing date	2022-07-21
Publishing country	England
Document type	Journal Article
ZDB-ID	432109-1
ISSN	1365-2761 ; 0140-7775
ISSN (online)	1365-2761
ISSN	0140-7775
DOI	10.1111/jfd.13687
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