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Article: Response to Comments on Transcriptional Changes in Cancer Cells Induced by Exposure to a Healing Method.

Beseme, Sarah / Bengston, William / Mc Michael, John

Dose-response : a publication of International Hormesis Society

2018 Volume 16, Issue 4, Page(s) 1559325818807780

Language	English
Publishing date	2018-10-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2440820-7
ISSN	1559-3258 ; 1559-3258
ISSN (online)	1559-3258
ISSN	1559-3258
DOI	10.1177/1559325818807780
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Effects of cannabis oil extract on immune response gene expression in human small airway epithelial cells (HSAEpC): implications for chronic obstructive pulmonary disease (COPD).

Mamber, Stephen W / Gurel, Volkan / Lins, Jeremy / Ferri, Fred / Beseme, Sarah / McMichael, John

Journal of cannabis research

2020 Volume 2, Issue 1, Page(s) 5

Abstract: Background: Chronic obstructive pulmonary disease (COPD) is commonly associated with both a pro-inflammatory and a T-helper 1 (Th1) immune response. It was hypothesized that cannabis oil extract can alleviate COPD symptoms by eliciting an anti- ... ...

Abstract	Background: Chronic obstructive pulmonary disease (COPD) is commonly associated with both a pro-inflammatory and a T-helper 1 (Th1) immune response. It was hypothesized that cannabis oil extract can alleviate COPD symptoms by eliciting an anti-inflammatory Th2 immune response. Accordingly, the effects of cannabis oil extract on the expression of 84 Th2 and related immune response genes in human small airways epithelial cells (HSAEpC) were investigated. Methods: HSAEpC from a single donor were treated with three dilutions of a standardized cannabis oil extract (1:400, 1:800 and 1:1600) along with a solvent control (0.25% [2.5 ul/ml] ethanol) for 24 h. There were four replicates per treatment dilution, and six for the control. RNA isolated from cells were employed in pathway-focused quantitative polymerase chain reaction (qPCR) microarray assays. Results: The extract induced significant (P < 0.05) changes in expression of 37 tested genes. Six genes (CSF2, IL1RL1, IL4, IL13RA2, IL17A and PPARG) were up-regulated at all three dilutions. Another two (CCL22 and TSLP) were up-regulated while six (CLCA1, CMA1, EPX, LTB4R, MAF and PMCH) were down-regulated at the 1:400 and 1:800 dilutions. The relationship of differentially-expressed genes of interest to biologic pathways was explored using the Database for Annotation, Visualization and Integrated Discovery (DAVID). Conclusions: This exploratory investigation indicates that cannabis oil extract may affect expression of specific airway epithelial cell genes that could modulate pro-inflammatory or Th1 processes in COPD. These results provide a basis for further investigations and have prompted in vivo studies of the effects of cannabis oil extract on pulmonary function. Trial registration: NONE (all in vitro experiments).
Language	English
Publishing date	2020-01-31
Publishing country	England
Document type	Journal Article
ISSN	2522-5782
ISSN (online)	2522-5782
DOI	10.1186/s42238-019-0014-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Effects Induced In Vivo by Exposure to Magnetic Signals Derived From a Healing Technique.

Beseme, Sarah / Fast, Loren / Bengston, William / Turner, Michael / Radin, Dean / McMichael, John

Dose-response : a publication of International Hormesis Society

2020 Volume 18, Issue 1, Page(s) 1559325820907741

Abstract: Energy healing is a therapy said to manipulate and balance the flow of "energies" in the body. One such technique, the Bengston Healing Method (BHM), has shown some success in healing malignant tumors in animals and humans, but the mechanism of action ... ...

Abstract	Energy healing is a therapy said to manipulate and balance the flow of "energies" in the body. One such technique, the Bengston Healing Method (BHM), has shown some success in healing malignant tumors in animals and humans, but the mechanism of action and factors influencing therapeutic success of this method are poorly understood. In this study, we tested in vivo the antitumor potential of magnetic signals recorded during BHM healing. Balb/c mice engrafted with 4T1 breast cancer cells were exposed to this recording for 4 h/d on a weekly or daily basis for 28 days; control mice were not exposed at all. Tumors showed a trend to grow slower in the treatment versus control group during the fourth week of treatment. Elevated leukocyte counts, associated with an increase in blood levels of granulocyte-macrophage colony stimulating factor and interleukin-6, were observed in tumor-bearing mice exposed to the BHM recording but not in healthy animals exposed to the recording. This suggests that exposure to a recording of BHM may induce a biological response in tumor-bearing mice, but limited effects on tumor growth when observed within the predefined end point of 28 days. Studies involving longer end points are recommended to observe the progression of tumor growth.
Language	English
Publishing date	2020-03-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2440820-7
ISSN	1559-3258
ISSN	1559-3258
DOI	10.1177/1559325820907741
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A story-telling cloth approach to motivating cervical cancer screening in Mali.

Crippin, Tiffani / Tounkara, Karamoko / Squibb, Eliza / Beseme, Sarah / Barry, Katherine / Sangare, Kotou / Coulibaly, Saidou / Fané, Pinda / Bagayoko, Aliou / Koita, Ousmane A / Teguété, Ibrahima / De Groot, Anne S

Frontiers in public health

2022 Volume 10, Page(s) 1051536

Abstract: Ninety percent of deaths from Cervical cancer (CC) caused by Human Papilloma Virus (HPV) occur in low- and middle-income countries. CC is the 2nd most common cause of cancer in women in West Africa, where 12,000 women develop cervical cancer and more ... ...

Abstract	Ninety percent of deaths from Cervical cancer (CC) caused by Human Papilloma Virus (HPV) occur in low- and middle-income countries. CC is the 2nd most common cause of cancer in women in West Africa, where 12,000 women develop cervical cancer and more than 6,000 die from the disease, annually. While HPV vaccination and CC screening have dramatically reduced the incidence of CC and mortality from CC in developed countries, prevention of CC in West Africa is often limited to visual inspection of the cervix and surgical intervention. In previous studies of CC in Mali, we demonstrated that knowledge about the link between HPV and CC is limited, and that screening for CC is often delayed until women are symptomatic. For this intervention, a story-telling cloth (West African-style printed pagne) was designed for use as a starting point for educational sessions run by community health workers. Community outreach using the cloth during 6 months of 2015 resulted in a 5-fold higher uptake of cervical cancer screening and increased awareness of the potential to vaccinate adolescents against CC. 3,271 women were motivated to visit one of five participating clinics for CC screening, where a mere 600 women had been screened during the previous year. This study shows that a comprehensive, visual, community-centered education campaign coupled with coordinated support for local clinics improves uptake of CC screening.
MeSH term(s)	Adolescent ; Humans ; Female ; Uterine Cervical Neoplasms/diagnosis ; Uterine Cervical Neoplasms/prevention & control ; Early Detection of Cancer/methods ; Mali ; Papillomavirus Infections/diagnosis ; Papillomavirus Infections/prevention & control ; Vaccination/adverse effects ; Human Papillomavirus Viruses
Language	English
Publishing date	2022-12-15
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2022.1051536
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Article: Transcriptional Changes in Cancer Cells Induced by Exposure to a Healing Method.

Beseme, Sarah / Bengston, William / Radin, Dean / Turner, Michael / McMichael, John

Dose-response : a publication of International Hormesis Society

2018 Volume 16, Issue 3, Page(s) 1559325818782843

Abstract: Energy healing, or healing with intent, is a complementary and alternative medicine therapy reported to be beneficial with a wide variety of conditions. We are developing a delivery technology for a method previously tested in mouse models with solid ... ...

Abstract	Energy healing, or healing with intent, is a complementary and alternative medicine therapy reported to be beneficial with a wide variety of conditions. We are developing a delivery technology for a method previously tested in mouse models with solid tumors (the Bengston method) independent of the presence of a healer. The goal of this study was to assess whether stored or recorded energy has an impact on breast cancer cells in vitro, using energy-charged cotton and electromagnetic recording of healers practicing the method. Expression of genes involved in cancer and inflammation pathways was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Treatment of cells using energy-charged cotton resulted in statistically significant changes <1.5-fold. In cells exposed to an electromagnetic recording, 37 genes of 167 tested showed a >1.5-fold change when compared to the control, and 68 genes showing statistically significant fold changes. Two genes, ATP citrate lyase (
Language	English
Publishing date	2018-07-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2440820-7
ISSN	1559-3258 ; 1559-3258
ISSN (online)	1559-3258
ISSN	1559-3258
DOI	10.1177/1559325818782843
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A story-telling cloth approach to motivating cervical cancer screening in Mali

Tiffani Crippin / Karamoko Tounkara / Eliza Squibb / Sarah Beseme / Katherine Barry / Kotou Sangare / Saidou Coulibaly / Pinda Fané / Aliou Bagayoko / Ousmane A. Koita / Ibrahima Teguété / Anne S. De Groot

Frontiers in Public Health, Vol

2022 Volume 10

Abstract	Ninety percent of deaths from Cervical cancer (CC) caused by Human Papilloma Virus (HPV) occur in low- and middle-income countries. CC is the 2nd most common cause of cancer in women in West Africa, where 12,000 women develop cervical cancer and more than 6,000 die from the disease, annually. While HPV vaccination and CC screening have dramatically reduced the incidence of CC and mortality from CC in developed countries, prevention of CC in West Africa is often limited to visual inspection of the cervix and surgical intervention. In previous studies of CC in Mali, we demonstrated that knowledge about the link between HPV and CC is limited, and that screening for CC is often delayed until women are symptomatic. For this intervention, a story-telling cloth (West African-style printed pagne) was designed for use as a starting point for educational sessions run by community health workers. Community outreach using the cloth during 6 months of 2015 resulted in a 5-fold higher uptake of cervical cancer screening and increased awareness of the potential to vaccinate adolescents against CC. 3,271 women were motivated to visit one of five participating clinics for CC screening, where a mere 600 women had been screened during the previous year. This study shows that a comprehensive, visual, community-centered education campaign coupled with coordinated support for local clinics improves uptake of CC screening.
Keywords	Human Papillomavirus ; cervical cancer ; West Africa ; infectious disease ; education ; screening ; Public aspects of medicine ; RA1-1270
Subject code	610
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online ; Conference proceedings: 8th vaccine renaissance: A creative nexus for vaccine developers.

Johnson, Heather / Moise, Lenny / Menge, Austin / Beseme, Sarah / De Groot, Anne S

Human vaccines & immunotherapeutics

2015 Volume 11, Issue 9, Page(s) 2294–2295

MeSH term(s)	Drug Approval ; Drug Discovery/methods ; Drug Discovery/trends ; Humans ; Vaccines/immunology ; Vaccines/isolation & purification
Chemical Substances	Vaccines
Language	English
Publishing date	2015
Publishing country	United States
Document type	Congresses ; Editorial
ZDB-ID	2664176-8
ISSN	2164-554X ; 2164-5515
ISSN (online)	2164-554X
ISSN	2164-5515
DOI	10.1080/21645515.2015.1069453
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Can Unconventional Immunomodulatory Agents Help Alleviate COVID-19 Symptoms and Severity?

Mamber, Stephen W / Krakowka, Steven / Osborn, Jeffrey / Saberski, Lloyd / Rhodes, Ryan G / Dahlberg, Albert E / Pond-Tor, Sunthorn / Fitzgerald, Kara / Wright, Neal / Beseme, Sarah / McMichael, John

mSphere

2020 Volume 5, Issue 3

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS coronavirus 2, or SARS-CoV-2) is the cause of the respiratory infection known as COVID-19. From an immunopathological standpoint, coronaviruses such as SARS-CoV-2 induce increased levels of a variety ... ...

Abstract	Severe acute respiratory syndrome coronavirus 2 (SARS coronavirus 2, or SARS-CoV-2) is the cause of the respiratory infection known as COVID-19. From an immunopathological standpoint, coronaviruses such as SARS-CoV-2 induce increased levels of a variety of T-helper 1 (Th1) and inflammatory cytokines and chemokines, including interleukin-1 (IL-1), IL-6, CCL2 protein, and CXCL10 protein. In the absence of proven antiviral agents or an effective vaccine, substances with immunomodulatory activity may be able to inhibit inflammatory and Th1 cytokines and/or yield an anti-inflammatory and/or Th2 immune response to counteract COVID-19 symptoms and severity. This report briefly describes the following four unconventional but commercially accessible immunomodulatory agents that can be employed in clinical trials to evaluate their effectiveness at alleviating disease symptoms and severity: low-dose oral interferon alpha, microdose DNA, low-dose thimerosal, and phytocannabinoids.
MeSH term(s)	Betacoronavirus ; COVID-19 ; Cannabinoids/therapeutic use ; Coronavirus Infections/drug therapy ; Cytokines/immunology ; DNA/therapeutic use ; Humans ; Immunomodulation ; Interferon-alpha/therapeutic use ; Pandemics ; Phytochemicals/therapeutic use ; Pneumonia, Viral/drug therapy ; SARS-CoV-2 ; Thimerosal/therapeutic use ; COVID-19 Drug Treatment
Chemical Substances	Cannabinoids ; Cytokines ; Interferon-alpha ; Phytochemicals ; Thimerosal (2225PI3MOV) ; DNA (9007-49-2)
Keywords	covid19
Language	English
Publishing date	2020-05-13
Publishing country	United States
Document type	Journal Article
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00288-20
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The ‘Story-telling Cloth’

Anne De Groot / Karamoko Tounkara / Eliza Squibb / Ibrahima Teguété / Aliou Bagayoko / Yssouf Kone / Kotou Sangare / Sarah Beseme / Ousmane Koita

Journal of Virus Eradication, Vol 4, Iss , Pp 36- (2018)

community-based education intervention for human papillomavirus vaccination in Bamako, Mali. A model for future HIV vaccination campaigns?

2018

Keywords	Microbiology ; QR1-502 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2018-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Healthy moms and healthy infants

Anne De Groot / Karamoko Tounkara / Lassina Diarra / Yssouf Kone / Zoumana Koty / Eliza Squibb / Sarah Beseme / Fanta Siby Diallo / Ousmane Koita

Journal of Virus Eradication, Vol 4, Iss , Pp 18-

12 years of successful mother-to-child HIV transmission prevention in a community health center in Bamako, Mali

2018 Volume 19

Keywords	Microbiology ; QR1-502 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2018-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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