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  1. Article ; Online: Maize requires Embryo defective27 for embryogenesis and seedling development.

    Liu, Xin-Yuan / Jiang, Rui-Cheng / Ma, Bing / Wang, Yong / Yang, Yan-Zhuo / Xu, Chunhui / Sun, Feng / Tan, Bao-Cai

    Plant physiology

    2024  Volume 195, Issue 1, Page(s) 430–445

    Abstract: The essential role of plastid translation in embryogenesis has been established in many plants, but a retrograde signal triggered by defective plastid translation machinery that may leads to embryogenesis arrest remains unknown. In this study, we ... ...

    Abstract The essential role of plastid translation in embryogenesis has been established in many plants, but a retrograde signal triggered by defective plastid translation machinery that may leads to embryogenesis arrest remains unknown. In this study, we characterized an embryo defective27 (emb27) mutant in maize (Zea mays), and cloning indicates that Emb27 encodes the plastid ribosomal protein S13. The null mutant emb27-1 conditions an emb phenotype with arrested embryogenesis; however, the leaky mutant emb27-2 exhibits normal embryogenesis but an albino seedling-lethal phenotype. The emb27-1/emb27-2 trans-heterozygotes display varying phenotypes from emb to normal seeds but albino seedlings. Analysis of the Emb27 transcription levels in these mutants revealed that the Emb27 expression level in the embryo corresponds with the phenotypic expression of the emb27 mutants. In the W22 genetic background, an Emb27 transcription level higher than 6% of the wild-type level renders normal embryogenesis, whereas lower than that arrests embryogenesis. Mutation of Emb27 reduces the level of plastid 16S rRNA and the accumulation of the plastid-encoded proteins. As a secondary effect, splicing of several plastid introns was impaired in emb27-1 and 2 other plastid translation-defective mutants, emb15 and emb16, suggesting that plastome-encoded factors are required for the splicing of these introns, such as Maturase K (MatK). Our results indicate that EMB27 is essential for plastid protein translation, embryogenesis, and seedling development in maize and reveal an expression threshold of Emb27 for maize embryogenesis.
    MeSH term(s) Zea mays/genetics ; Zea mays/embryology ; Seedlings/genetics ; Seedlings/growth & development ; Seeds/genetics ; Seeds/growth & development ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Gene Expression Regulation, Plant ; Mutation/genetics ; Plastids/genetics ; Plastids/metabolism ; Phenotype ; RNA Splicing/genetics ; Introns/genetics ; Ribosomal Proteins/genetics ; Ribosomal Proteins/metabolism
    Chemical Substances Plant Proteins ; Ribosomal Proteins
    Language English
    Publishing date 2024-01-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1093/plphys/kiae010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Online: Surface wrinkling of a film coated to a graded substrate

    Liu, Rui-Cheng / Liu, Yang / Goriely, Alain

    2023  

    Abstract: We study the surface wrinkling of a stiff thin elastic film bonded to a compliant graded elastic substrate subject to compressive stress generated either by compression or growth of the bilayer. Our aim is to clarify the influence of the modulus gradient ...

    Abstract We study the surface wrinkling of a stiff thin elastic film bonded to a compliant graded elastic substrate subject to compressive stress generated either by compression or growth of the bilayer. Our aim is to clarify the influence of the modulus gradient on the onset and surface pattern in this bilayers. Within the framework of finite elasticity, an exact bifurcation condition is obtained using the Stroh formulation and the surface impedance matrix method. Further analytical progress is made by focusing on the case of short wavelength limit for which the Wentzel-Kramers-Brillouin method can be used to resolve the eigenvalue problem of ordinary differential equations with variable coefficients. An explicit bifurcation condition is obtained from which asymptotic the critical buckling load and the critical wavelength are derived. In particular, we consider two distinct situations depending on the ratio $\beta$ of the shear modulus at the substrate surface to that at infinity. If $\beta$ is of $\mathcal{O}(1)$ or small, the parameters related to modulus gradient all appear in the high order terms and play an insignificant role in the bifurcation. In that case, it is the modulus ratio between the film and substrate surface that governs the onset of surface wrinkling. If, however, $\beta\gg1$, the modulus gradient affects the critical condition through leading-order terms. Through our analysis we unravel the influence of different material and geometric parameters, including the modulus gradient, on the bifurcation threshold and the associated wavelength which can be of importance in many biological and technological settings.
    Keywords Condensed Matter - Materials Science
    Publishing date 2023-12-15
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: NOA61-Polymer Fiber Fizeau Interferometer with a Flexible NOA65-Polymer Taper for Simultaneous Measurement of Tilt Angle and Temperature.

    Lee, Cheng-Ling / Chen, Chi-Shiang / Yang, Chun-Ren / Zeng, Rui-Cheng

    Polymers

    2021  Volume 13, Issue 16

    Abstract: This study proposes and experimentally demonstrates a NOA61-polymer fiber Fizeau interferometer (PFFI) connected to a flexible NOA65-polymer taper (PT) for simultaneous measurement of tilt angle and temperature (T). The PT/PFFI fiber sensor consists of a ...

    Abstract This study proposes and experimentally demonstrates a NOA61-polymer fiber Fizeau interferometer (PFFI) connected to a flexible NOA65-polymer taper (PT) for simultaneous measurement of tilt angle and temperature (T). The PT/PFFI fiber sensor consists of a taper-shaped flexible NOA65 polymer and single-mode fiber with an endface that is attached to a NOA61-polymer. The NOA61-polymer of PFFI is highly sensitive to variations of T with high repeatability and enables the simultaneous measurement of tilt angle by connecting with the highly flexible NOA65-PT. the interference fringe visibility of optical spectra in the PFFI can be highly controlled by the tilt angle of the PT and is thus capable of measuring tilt angles with high sensitivity. On the other hand, wavelength shifts of the spectra in the PFFI only occur when T varies. The proposed PT/PFFI can simultaneously detect the tilt state and the variation of surrounding T by measuring the optical spectral responses and eliminating cross sensitivity. Experimental results demonstrate the PT/PFFI can simultaneously measure tilt angles and T with good sensitivities and obtain averages of 0.4 dB/° and 0.17 nm/°C, respectively.
    Language English
    Publishing date 2021-08-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym13162798
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: NOA61-Polymer Fiber Fizeau Interferometer with a Flexible NOA65-Polymer Taper for Simultaneous Measurement of Tilt Angle and Temperature

    Cheng-Ling Lee / Chi-Shiang Chen / Chun-Ren Yang / Rui-Cheng Zeng

    Polymers, Vol 13, Iss 2798, p

    2021  Volume 2798

    Abstract: This study proposes and experimentally demonstrates a NOA61-polymer fiber Fizeau interferometer (PFFI) connected to a flexible NOA65-polymer taper (PT) for simultaneous measurement of tilt angle and temperature (T). The PT/PFFI fiber sensor consists of a ...

    Abstract This study proposes and experimentally demonstrates a NOA61-polymer fiber Fizeau interferometer (PFFI) connected to a flexible NOA65-polymer taper (PT) for simultaneous measurement of tilt angle and temperature (T). The PT/PFFI fiber sensor consists of a taper-shaped flexible NOA65 polymer and single-mode fiber with an endface that is attached to a NOA61-polymer. The NOA61-polymer of PFFI is highly sensitive to variations of T with high repeatability and enables the simultaneous measurement of tilt angle by connecting with the highly flexible NOA65-PT. the interference fringe visibility of optical spectra in the PFFI can be highly controlled by the tilt angle of the PT and is thus capable of measuring tilt angles with high sensitivity. On the other hand, wavelength shifts of the spectra in the PFFI only occur when T varies. The proposed PT/PFFI can simultaneously detect the tilt state and the variation of surrounding T by measuring the optical spectral responses and eliminating cross sensitivity. Experimental results demonstrate the PT/PFFI can simultaneously measure tilt angles and T with good sensitivities and obtain averages of 0.4 dB/° and 0.17 nm/°C, respectively.
    Keywords fiber tilt sensor ; NOA61 ; NOA65 ; polymer ; simultaneous measurement ; fiber Fizeau interferometer ; Organic chemistry ; QD241-441
    Subject code 660
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Determining postoperative analgesic efficacy of a regional block for pediatric patients.

    Huang, Yang-Xin-Rui / Li, Cheng-Wen / Xue, Fu-Shan

    Paediatric anaesthesia

    2024  Volume 34, Issue 5, Page(s) 483–484

    MeSH term(s) Humans ; Child ; Anesthesia, Conduction ; Analgesics ; Pain, Postoperative/drug therapy
    Chemical Substances Analgesics
    Language English
    Publishing date 2024-02-22
    Publishing country France
    Document type Letter ; Comment
    ZDB-ID 1086049-6
    ISSN 1460-9592 ; 1155-5645
    ISSN (online) 1460-9592
    ISSN 1155-5645
    DOI 10.1111/pan.14862
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Online: Influence of the growth gradient on surface wrinkling and pattern transition in growing tubular tissues

    Liu, Rui-Cheng / Liu, Yang / Cai, Zongxi

    2021  

    Abstract: Growth-induced pattern formations in curved film-substrate structures have attracted extensive attentions recently. In most existing literature, the growth tensor is assumed to be homogeneous or piecewise homogeneous. In this paper, we aim at clarifying ... ...

    Abstract Growth-induced pattern formations in curved film-substrate structures have attracted extensive attentions recently. In most existing literature, the growth tensor is assumed to be homogeneous or piecewise homogeneous. In this paper, we aim at clarifying the influence of a growth gradient on pattern formation and pattern evolution in bilayered tubular tissues under plane-strain deformation. In the framework of finite elasticity, a bifurcation condition is derived for a general material model and a generic growth function. Then we suppose that both layers are composed of neo-Hookean materials. In particular, the growth function is assumed to decay linearly either from the inner surface or from the outer surface. It is found that a gradient in the growth has a weak effect on the critical state, compared to the homogeneous growth type where both layers share the same growth factor. Furthermore, a finite element model is built to validate the theoretical model and to investigate the post-buckling behaviors. It is found that the associated pattern transition is not controlled by the growth gradient but by the ratio of the shear modulus between two layers. Different morphologies can occur when the modulus ratio is varied. The current analysis could provide useful insight into the influence of a growth gradient on surface instabilities and suggests that a homogeneous growth field may provide a good approximation on interpreting complicated morphological formations in multiple systems.

    Comment: 21 pages, 12 figures
    Keywords Nonlinear Sciences - Pattern Formation and Solitons ; Condensed Matter - Soft Condensed Matter
    Subject code 530 ; 612
    Publishing date 2021-09-15
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Transcriptional landscape of human neuroblastoma cells in response to SARS-CoV-2.

    Yang, Rui-Cheng / Huang, Kun / Zhang, Hui-Peng / Li, Liang / Tan, Chen / Chen, Huan-Chun / Jin, Mei-Lin / Wang, Xiang-Ru

    BMC neuroscience

    2022  Volume 23, Issue 1, Page(s) 43

    Abstract: Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and the neurological symptoms of SARS-CoV-2 infection have already been reported. However, the mechanisms underlying the effect of SARS-CoV-2 infection on ... ...

    Abstract Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and the neurological symptoms of SARS-CoV-2 infection have already been reported. However, the mechanisms underlying the effect of SARS-CoV-2 infection on patients with central nervous system injuries remain unclear.
    Methods: The high-throughput RNA sequencing was applied to analyze the transcriptomic changes in SK-N-SH cells after SARS-CoV-2 infection. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to identify the functions of differentially expressed genes and related pathways.
    Results: A total of 820 mRNAs were significantly altered, including 671 upregulated and 149 downregulated mRNAs (showing an increase of ≥ 2-fold or decrease to ≤ 0.5-fold, respectively; p ≤ 0.05). Moreover, we verified the significant induction of cytokines, chemokines, and their receptors, as well as the activation of NF-κB, p38, and Akt signaling pathways, in SK-N-SH by SARS-CoV-2.
    Conclusions: To our knowledge, this is the first time the transcriptional profiles of the host mRNAs involved in SARS-CoV-2 infection of SK-N-SH cells have been reported. These findings provide novel insight into the pathogenic mechanism of SARS-CoV-2 and might constitute a new approach for future prevention and treatment of SARS-CoV-2-induced central nervous system infection.
    MeSH term(s) COVID-19 ; Cytokines ; Humans ; NF-kappa B ; Neuroblastoma ; RNA, Messenger/metabolism ; SARS-CoV-2
    Chemical Substances Cytokines ; NF-kappa B ; RNA, Messenger
    Language English
    Publishing date 2022-07-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2041344-0
    ISSN 1471-2202 ; 1471-2202
    ISSN (online) 1471-2202
    ISSN 1471-2202
    DOI 10.1186/s12868-022-00728-6
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  8. Article ; Online: Passively Q-switched 2.3 μm Tm:YVO

    Zhao, Jiaqun / Yang, Xinyuan / Liu, Yuantong / Jin, Lei / Yu, Rui / Zhou, Xiaofeng / Cheng, Ping

    Optics letters

    2024  Volume 49, Issue 4, Page(s) 862–865

    Abstract: A diode-pumped wavelength switchable passively Q-switched 2.3 μm Tm: ... ...

    Abstract A diode-pumped wavelength switchable passively Q-switched 2.3 μm Tm:YVO
    Language English
    Publishing date 2024-02-07
    Publishing country United States
    Document type Journal Article
    ISSN 1539-4794
    ISSN (online) 1539-4794
    DOI 10.1364/OL.516389
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Analysis of Phase Mismatch for Mercurous Bromide-Based Non-Collinear AOTF Design in Spectral Imaging Applications.

    Zhao, Huijie / Cheng, Chi / Guo, Qi / Ma, Rui / Yang, Yutian

    Materials (Basel, Switzerland)

    2024  Volume 17, Issue 7

    Abstract: The spectral and spatial characteristics of Acousto-Optic Tunable Filters (AOTFs), such as a tuning curve, spectral resolution, angular aperture, and diffraction efficiency, are determined by the device's acousto-optic crystal configuration and ... ...

    Abstract The spectral and spatial characteristics of Acousto-Optic Tunable Filters (AOTFs), such as a tuning curve, spectral resolution, angular aperture, and diffraction efficiency, are determined by the device's acousto-optic crystal configuration and piezoelectric transducer. For high-throughput spectral imaging applications, it is essential to enlarge the spectral bandwidth and angular aperture during the design phase of AOTFs. Thus, phase mismatch due to incident angle or wavelength was studied analytically using phase diagrams in this paper. Additionally, a performance parameter analysis model was established based on the use of mercurous bromide crystals for large angular aperture AOTF device design, and the impact of crystal and transducer design parameters on the spectral bandwidth and angular aperture was evaluated. This also experimentally validates the diffraction capability of AOTFs made from mercurous bromide crystal, which possess a broad spectral transmission ability ranging from visible to long-wave infrared.
    Language English
    Publishing date 2024-04-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma17071703
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  10. Article ; Online: Protocol for quantitative evaluation of misfolded protein degradation using Agrobacterium-mediated expression system in Nicotiana benthamiana.

    Cheng, Yang / Meng, Rui / Niu, Siqi / Peng, Hao / Jing, Maofeng

    STAR protocols

    2024  Volume 5, Issue 2, Page(s) 103034

    Abstract: Cellular protein homeostasis is maintained by the disposal of aggregated misfolded proteins. Here, we present a protocol for investigating the involvement of the proteins of interest in misfolded protein degradation via Agrobacterium-mediated transient ... ...

    Abstract Cellular protein homeostasis is maintained by the disposal of aggregated misfolded proteins. Here, we present a protocol for investigating the involvement of the proteins of interest in misfolded protein degradation via Agrobacterium-mediated transient expression in Nicotiana benthamiana. We describe in detail the steps of misfolded protein design, transient protein expression in N. benthamiana, subsequent total protein extraction, and quantification of misfolded proteins through western blotting. This generalizable system can be used for misfolded proteins derived from various plants or microbes. For complete details on the use and execution of this protocol, please refer to Ai et al.
    Language English
    Publishing date 2024-04-24
    Publishing country United States
    Document type Journal Article
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2024.103034
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