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Article ; Online: Liang-Ge-San

Liling Yang / Lijun Yan / Weifu Tan / Xiangjun Zhou / Guangli Yang / Jingtao Yu / Zibin Lu / Yong Liu / Liyi Zou / Wei Li / Linzhong Yu

Frontiers in Pharmacology, Vol

a classic traditional Chinese medicine formula, attenuates acute inflammation via targeting GSK3β

2023 Volume 14

Abstract: ... that burden the world, but there is still a lack of more effective and reliable drug treatment. Liang-Ge-San ...

Abstract	Sepsis is a serious life-threatening health disorder with high morbidity and mortality rates that burden the world, but there is still a lack of more effective and reliable drug treatment. Liang-Ge-San (LGS) has been shown to have anti-inflammatory effects and is a promising candidate for the treatment of sepsis. However, the anti-sepsis mechanism of LGS has still not been elucidated. In this study, a set of genes related to inflammatory chemotaxis pathways was downloaded from Encyclopedia of Genes and Genomes (KEGG) and integrated with sepsis patient information from the Gene Expression Omnibus (GEO) database to perform differential gene expression analysis. Glycogen synthase kinase-3β (GSK-3β) was found to be the feature gene after these important genes were examined using the three algorithms Random Forest, support vector machine recursive feature elimination (SVM-REF), and least absolute shrinkage and selection operator (LASSO), and then intersected with possible treatment targets of LGS found through the search. Upon evaluation, the receiver operating characteristic (ROC) curve of GSK-3β indicated an important role in the pathogenesis of sepsis. Immune cell infiltration analysis suggested that GSK-3β expression was associated with a variety of immune cells, including neutrophils and monocytes. Next, lipopolysaccharide (LPS)-induced zebrafish inflammation model and macrophage inflammation model was used to validate the mechanism of LGS. We found that LGS could protect zebrafish against a lethal challenge with LPS by down-regulating GSK-3β mRNA expression in a dose-dependent manner, as indicated by a decreased neutrophils infiltration and reduction of inflammatory damage. The upregulated mRNA expression of GSK-3β in LPS-induced stimulated RAW 264.7 cells also showed the same tendency of depression by LGS. Critically, LGS could induce M1 macrophage polarization to M2 through promoting GSK-3β inactivation of phosphorylation. Taken together, we initially showed that anti-septic effects of LGS is related to the ...
Keywords	Liang-Ge-San ; traditional Chinese medicine ; sepsis ; acute inflammation ; LPS ; GSK-3β ; Therapeutics. Pharmacology ; RM1-950
Subject code	570
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Liang-Ge-San: a classic traditional Chinese medicine formula, attenuates acute inflammation via targeting GSK3β.

Yang, Liling / Yan, Lijun / Tan, Weifu / Zhou, Xiangjun / Yang, Guangli / Yu, Jingtao / Lu, Zibin / Liu, Yong / Zou, Liyi / Li, Wei / Yu, Linzhong

Frontiers in pharmacology

2023 Volume 14, Page(s) 1181319

Abstract: ... that burden the world, but there is still a lack of more effective and reliable drug treatment. Liang-Ge-San ...

Abstract	Sepsis is a serious life-threatening health disorder with high morbidity and mortality rates that burden the world, but there is still a lack of more effective and reliable drug treatment. Liang-Ge-San (LGS) has been shown to have anti-inflammatory effects and is a promising candidate for the treatment of sepsis. However, the anti-sepsis mechanism of LGS has still not been elucidated. In this study, a set of genes related to inflammatory chemotaxis pathways was downloaded from Encyclopedia of Genes and Genomes (KEGG) and integrated with sepsis patient information from the Gene Expression Omnibus (GEO) database to perform differential gene expression analysis. Glycogen synthase kinase-3β (GSK-3β) was found to be the feature gene after these important genes were examined using the three algorithms Random Forest, support vector machine recursive feature elimination (SVM-REF), and least absolute shrinkage and selection operator (LASSO), and then intersected with possible treatment targets of LGS found through the search. Upon evaluation, the receiver operating characteristic (ROC) curve of GSK-3β indicated an important role in the pathogenesis of sepsis. Immune cell infiltration analysis suggested that GSK-3β expression was associated with a variety of immune cells, including neutrophils and monocytes. Next, lipopolysaccharide (LPS)-induced zebrafish inflammation model and macrophage inflammation model was used to validate the mechanism of LGS. We found that LGS could protect zebrafish against a lethal challenge with LPS by down-regulating GSK-3β mRNA expression in a dose-dependent manner, as indicated by a decreased neutrophils infiltration and reduction of inflammatory damage. The upregulated mRNA expression of GSK-3β in LPS-induced stimulated RAW 264.7 cells also showed the same tendency of depression by LGS. Critically, LGS could induce M1 macrophage polarization to M2 through promoting GSK-3β inactivation of phosphorylation. Taken together, we initially showed that anti-septic effects of LGS is related to the inhibition on GSK-3β, both
Language	English
Publishing date	2023-06-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2023.1181319
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Article ; Online: Study on the mechanism of Gao-Liang-Huo decoction against dextran sulfate sodium-induced ulcerative colitis based on metabolomics and network pharmacology

Qiang Tian / Pei Hu / Miaohua Wang / Yu Hu / Like Xie / Zhixiong Li / Zhou Xu / Fang Liu / Chenggang Huang

Pharmacological Research - Modern Chinese Medicine, Vol 9, Iss , Pp 100304- (2023)

2023

Abstract: Backgrounds: Gao-Liang-Huo decoction (GLH), a traditional Chinese medicine consisting of gao ...

Abstract	Backgrounds: Gao-Liang-Huo decoction (GLH), a traditional Chinese medicine consisting of gao liangjiang (the roots or tubers of Alpinia offinarum Hance) and guang huoxiang (the aerial parts of Pogostemon cablin (Blanco) Benth.), has been shown an effective therapeutic efficacy for ulcerative colitis (UC). However, the active compounds and underlying mechanisms involved in GLH treatment of UC remains unclear. This study aims to investigate the pharmacological mechanism of GLH on UC using an integrated strategy of metabolomics and network pharmacology. Methods: The experimental mice UC model was induced using dextran sulfate sodium (DSS). The disease activity index (DAI), colonic mucosa damage index (CMDI), biochemical assays, and histopathological analysis were used to evaluate the pharmacological effect of GLH. The LC/MS-based metabolomics was used for investigation of serum metabolites and metabolic pathways regulated by GLH. The potential targets and pathways of the active compounds in GLH were further explored using network pharmacology. Finally, the underlying mechanism of GLH on UC was verified by molecular docking and immunohistochemistry (IHC). Results: The pharmacological experiments showed that GLH significantly ameliorated the DAI, CMDI, MDA, MPO, and histopathological changes in DSS-induced UC. The 15 metabolites were identified by metabolomics, such as L-tryptophan, taurocholic acid, glycocholic acid, 3-indolebutyric acid, and tryptophol. These metabolites were found to be enriched in tryptophan metabolism, glycerophospholipid metabolism, and citrate cycle (TCA cycle). The results of network pharmacology revealed the key targets, such as PIK3CA, PIK3R1, JAK1, JAK2, and JAK3, mainly involved in the PI3K-AKT, JAK-STAT, and T-cell receptor signaling pathways. Molecular docking showed high affinity interaction of quercetin, isorhamnrtin, poriferast-5-en-3beta-ol, 1,7-diphenyl-5-hydroxy-3-heptanone, and medicarpin with PIK3CA, PIK3R1, JAK1, JAK2, and JAK3, respectively. Finally, the immunohistochemistry ...
Keywords	Gao-Liang-Huo decoction ; Ulcerative colitis ; Network pharmacology ; Metabolomics ; Molecular mechanism ; Other systems of medicine ; RZ201-999 ; Therapeutics. Pharmacology ; RM1-950
Subject code	570
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Traditional Chinese medicine (Liang-Xue-Di-Huang Decoction) for hemorrhoid hemorrhage: Study Protocol Clinical Trial (SPIRIT Compliant).

Shi, Shuo-Yang / Zhou, Qing / He, Zong-Qi / Shen, Zhao-Feng / Zhang, Wei-Xin / Zhang, Dan / Xu, Cheng-Biao / Geng, Ji / Wu, Ben-Sheng / Chen, Yu-Gen

Medicine

2020 Volume 99, Issue 16, Page(s) e19720

Abstract: ... in the general population. Medical therapy for HD has not been formally confirmed due to the inconsistent of results. Liang ... from the 19th century in China. However, clinical research of Liang-Xue-Di-Huang Decoction in the treatment ... of HD was lack. We designed this study to evaluate the efficacy and safety of Liang-Xue-Di-Huang ...

Abstract	Background: Hemorrhoidal disease (HD) is one of the commonest proctologic condition in the general population. Medical therapy for HD has not been formally confirmed due to the inconsistent of results. Liang-Xue-Di-Huang Decoction, a kind of ancient Chinese classical prescription, has been used to treat HD from the 19th century in China. However, clinical research of Liang-Xue-Di-Huang Decoction in the treatment of HD was lack. We designed this study to evaluate the efficacy and safety of Liang-Xue-Di-Huang Decoction in the treatment of HD. Methods/design: A randomized, controlled, double blind, double-mimetic agent, and multicenter trial to evaluate the efficacy and safety of Liang-Xue-Di-Huang Decoction is proposed. HD patients (stage I, II, III) will be randomly assigned into experimental group or control group. HD patients will receive a 7-day treatments and a 7-day follow-up. The primary outcome measure is the Hemorrhoid Bleeding Score in 7 and 14 days. The Secondary outcome measures are Goligher prolapse score and quality-of-life score in 7 and 14 days. Discussion: This study will provide objective evidences to evaluate the efficacy and safety of Liang-Xue-Di-Huang Decoction in treatment of HD.
MeSH term(s)	Humans ; Drugs, Chinese Herbal/therapeutic use ; Hemorrhage/drug therapy ; Hemorrhage/etiology ; Hemorrhoids/complications ; Hemorrhoids/drug therapy ; Medicine, Chinese Traditional ; Phytotherapy ; Multicenter Studies as Topic ; Randomized Controlled Trials as Topic
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2020-04-17
Publishing country	United States
Document type	Clinical Trial Protocol ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000019720
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21.

Yang, Huayi / Lu, Zibin / Huo, Chuying / Chen, Yuyao / Cao, Huihui / Xie, Pei / Zhou, Hongling / Liu, Dongyi / Liu, Junshan / Yu, Linzhong

Frontiers in pharmacology

2019 Volume 10, Page(s) 1332

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2019-11-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2019.01332
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Article ; Online: Liang-Ge-San, a classic traditional Chinese medicine formula, attenuates acute inflammation in zebrafish and RAW 264.7 cells.

Zhou, Hongling / Cao, Huihui / Zheng, Yuanru / Lu, Zibin / Chen, Yuyao / Liu, Dongyi / Yang, Huayi / Quan, Jingyu / Huo, Chuying / Liu, Junshan / Yu, Linzhong

Journal of ethnopharmacology

2019 Volume 249, Page(s) 112427

Abstract: Ethnopharmacological relevance: Liang-Ge-San (LGS) is a traditional Chinese medicine formula ...

Abstract	Ethnopharmacological relevance: Liang-Ge-San (LGS) is a traditional Chinese medicine formula that commonly used in acute inflammatory diseases. However, the anti-inflammatory effects and the underlying mechanisms of LGS are not fully studied. Aim of the study: This study aims to investigate the anti-inflammatory activity and explore the underlying mechanisms of LGS in zebrafish and cell inflammation models. Materials and methods: LPS-induced zebrafish inflammation model was established by LPS-yolk microinjection. The protective effect of LGS on zebrafish injected with LPS was observed using survival analysis. Infiltration of inflammatory cells was determined by H&E staining assay. Expression levels of key inflammatory cytokines TNF-α and IL-6 were measured by q-PCR assay. Recruitment of neutrophils and macrophages were observed by fluorescence microscopy, SB staining and NR staining. In vitro anti-inflammatory effects of LGS were evaluated on LPS-stimulated RAW 264.7 cells. The generation of IL-6 and TNF-α was detected by ELISA. The protein expression levels of JNK, p-JNK (Thr183/Tyr185), Nur77 and p-Nur77 (Ser351) were determined by Western blotting. Finally, two additional inflammatory models in zebrafish, which were induced by CuSO Results: LGS protected zebrafish against LPS-induced death and dose-dependently inhibited LPS-induced acute inflammatory response in zebrafish, as indicated by increased survival rate, reduced infiltration of inflammatory cells, decreased recruitment of macrophages and neutrophils, and downregulated expression levels of TNF-α and IL-6. Additionally, LGS inhibited the secretion of TNF-α and IL-6, increased the expression of Nur77, and reduced the expression of p-Nur77 (Ser351) and p-JNK (Thr183/Tyr185) in LPS-stimulated RAW 264.7 cells. The anti-inflammatory action of LGS was also observed in another two zebrafish inflammation models, which was supported by the inhibition on neutrophils and macrophages recruitment. Conclusion: The present study demonstrates that LGS possesses anti-inflammatory activity in zebrafish inflammation models and LPS-stimulated RAW 264.7 cells, which is related to the inhibition on p-JNK and p-Nur77. This finding provides a pharmacological basis for LGS in the control of inflammatory disorder.
MeSH term(s)	Acute Disease/therapy ; Animals ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Disease Models, Animal ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Female ; Humans ; Inflammation/drug therapy ; Inflammation/immunology ; Inflammation Mediators/immunology ; Inflammation Mediators/metabolism ; JNK Mitogen-Activated Protein Kinases/immunology ; JNK Mitogen-Activated Protein Kinases/metabolism ; MAP Kinase Signaling System/drug effects ; MAP Kinase Signaling System/immunology ; Macrophages/drug effects ; Macrophages/immunology ; Male ; Mice ; Neutrophils/drug effects ; Neutrophils/immunology ; Nuclear Receptor Subfamily 4, Group A, Member 1/immunology ; Nuclear Receptor Subfamily 4, Group A, Member 1/metabolism ; Phosphorylation/drug effects ; RAW 264.7 Cells ; Zebrafish
Chemical Substances	Anti-Inflammatory Agents ; Drugs, Chinese Herbal ; Inflammation Mediators ; Nuclear Receptor Subfamily 4, Group A, Member 1 ; JNK Mitogen-Activated Protein Kinases (EC 2.7.11.24)
Language	English
Publishing date	2019-11-25
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2019.112427
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Article: Liang-Ge-San, a classic traditional Chinese medicine formula, attenuates acute inflammation in zebrafish and RAW 264.7 cells

Zhou, Hongling / Cao, Huihui / Chen, Yuyao / Huo, Chuying / Liu, Dongyi / Liu, Junshan / Lu, Zibin / Quan, Jingyu / Yang, Huayi / Yu, Linzhong / Zheng, Yuanru

Journal of ethnopharmacology. 2020 Mar. 01, v. 249

2020

Abstract: Liang-Ge-San (LGS) is a traditional Chinese medicine formula that commonly used in acute ...

Abstract	Liang-Ge-San (LGS) is a traditional Chinese medicine formula that commonly used in acute inflammatory diseases. However, the anti-inflammatory effects and the underlying mechanisms of LGS are not fully studied.This study aims to investigate the anti-inflammatory activity and explore the underlying mechanisms of LGS in zebrafish and cell inflammation models.LPS-induced zebrafish inflammation model was established by LPS-yolk microinjection. The protective effect of LGS on zebrafish injected with LPS was observed using survival analysis. Infiltration of inflammatory cells was determined by H&E staining assay. Expression levels of key inflammatory cytokines TNF-α and IL-6 were measured by q-PCR assay. Recruitment of neutrophils and macrophages were observed by fluorescence microscopy, SB staining and NR staining. In vitro anti-inflammatory effects of LGS were evaluated on LPS-stimulated RAW 264.7 cells. The generation of IL-6 and TNF-α was detected by ELISA. The protein expression levels of JNK, p-JNK (Thr183/Tyr185), Nur77 and p-Nur77 (Ser351) were determined by Western blotting. Finally, two additional inflammatory models in zebrafish, which were induced by CuSO4 or tail fin injury, were also established and the recruitment of neutrophils and macrophages were observed for the determination of the anti-inflammatory activity of LGS.LGS protected zebrafish against LPS-induced death and dose-dependently inhibited LPS-induced acute inflammatory response in zebrafish, as indicated by increased survival rate, reduced infiltration of inflammatory cells, decreased recruitment of macrophages and neutrophils, and downregulated expression levels of TNF-α and IL-6. Additionally, LGS inhibited the secretion of TNF-α and IL-6, increased the expression of Nur77, and reduced the expression of p-Nur77 (Ser351) and p-JNK (Thr183/Tyr185) in LPS-stimulated RAW 264.7 cells. The anti-inflammatory action of LGS was also observed in another two zebrafish inflammation models, which was supported by the inhibition on neutrophils and macrophages recruitment.The present study demonstrates that LGS possesses anti-inflammatory activity in zebrafish inflammation models and LPS-stimulated RAW 264.7 cells, which is related to the inhibition on p-JNK and p-Nur77. This finding provides a pharmacological basis for LGS in the control of inflammatory disorder.
Keywords	anti-inflammatory activity ; copper sulfate ; Danio rerio ; death ; enzyme-linked immunosorbent assay ; fluorescence microscopy ; inflammation ; interleukin-6 ; macrophages ; mitogen-activated protein kinase ; models ; neutrophils ; Oriental traditional medicine ; protective effect ; protein synthesis ; secretion ; staining ; survival rate ; tail ; tumor necrosis factor-alpha ; Western blotting
Language	English
Dates of publication	2020-0301
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2019.112427
Database	NAL-Catalogue (AGRICOLA)

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Article: Response to Liang Hong and Ryan Martin on their comments on our paper entitled, "credibility estimation of distribution functions with applications to experience rating in general insurance"

Cai, Xiaoqiang / Hong, Liang / Martin, Ryan / Wen, Limin / Wu, Xianyi / Zhou, Xian

North American actuarial journal Vol. 20, No. 1 , p. 99-100

2016 Volume 20, Issue 1, Page(s) 99–100

Author's details	Xiaoqiang Cai, Limin Wen, Xianyi Wu, and Xian Zhou
Language	English
Publisher	Taylor & Francis
Publishing place	Philadelphia, Pa
Document type	Article
ZDB-ID	1380138-7
ISSN	1092-0277
Database	ECONomics Information System

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Article ; Online: Liang-Ge-San, a classic traditional Chinese medicine formula, protects against lipopolysaccharide-induced inflammation through cholinergic anti-inflammatory pathway.

Liu, Jun-Shan / Wei, Xi-Duan / Lu, Zi-Bin / Xie, Pei / Zhou, Hong-Ling / Chen, Yu-Yao / Ma, Jia-Mei / Yu, Lin-Zhong

Oncotarget

2016 Volume 7, Issue 16, Page(s) 21222–21234

Abstract: Liang-Ge-San (LGS) is a classic formula in traditional Chinese medicine, which is widely used ...

Abstract	Liang-Ge-San (LGS) is a classic formula in traditional Chinese medicine, which is widely used to treat acute lung injury (ALI), pharyngitis and amygdalitis in clinic. However, the underlying mechanisms remain poorly defined. In this study, we discovered that LGS exerted potent anti-inflammatory effects in lipopolysaccharide (LPS)-induced inflammation. We found that LGS significantly depressed the production of IL-6 and TNF-α in LPS-stimulated RAW 264.7 macrophage cells. The degradation and phosphorylation of IκBα and the nuclear translocation of NF-κB p65 were also inhibited. Moreover, LGS activated α7 nicotinic cholinergic receptor (α7nAchR). The blockage of α7nAchR by selective inhibitor methyllycaconitine (MLA) or α7nAchR siRNA attenuated the inhibitory effects of LGS on IκBα, NF-κB p65, IL-6 and TNF-α. Critically, LGS significantly inhibited inflammation in LPS-induced ALI rats through the activation of NF-κB signaling pathway. However, these protective effects could be counteracted by the treatment of MLA. Taken together, we first demonstrated anti-inflammatory effects of LGS both in vitro and in vivo through cholinergic anti-inflammatory pathway. The study provides a rationale for the clinical application of LGS as an anti-inflammatory agent and supports the critical role of cholinergic anti-inflammatory pathway in inflammation.
Language	English
Publishing date	2016-04-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.8452
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Article ; Online: Effects of modified Shu-Gan-Liang-Xue decoction combined with anastrozole on osteoblastic proliferation and differentiation of MC3T3-E1 cells.

Zhou, Fei / Han, Shuyan / Zhou, Ning / Zheng, Wenxian / Li, Pingping

Molecular medicine reports

2014 Volume 11, Issue 3, Page(s) 1639–1646

Abstract: ... receiving AI treatment. Modified Shu‑Gan‑Liang‑Xue decoction (mSGLXD), a clinical prescription, has been ...

Abstract	Aromatase inhibitors (AIs) are widely used in the treatment of hormone‑dependent breast cancer and as a result, aromatase inhibitor‑associated bone loss (AIBL) has become a major concern amongst patients receiving AI treatment. Modified Shu‑Gan‑Liang‑Xue decoction (mSGLXD), a clinical prescription, has been used for ameliorating AIBL in patients with breast cancer for decades and has achieved good clinical efficacy. However, the mechanism underlying how mSGLXD influences bone homeostasis and alleviates AIBL has remained elusive. In the present study, mSGLXD was supplemented with Rhizoma Drynariae containing phytoestrogens, and the safety of mSGLXD was evaluated. mSGLXD did not possess estrogenic activity and significantly inhibited the proliferation of estrogen receptor‑positive breast cancer cell line MCF‑7, which suggested that mSGLXD was safe for postmenopausal patients with breast cancer. Subsequently, the effects of mSGLXD alone or in combination with anastrozole on osteoblastic MC3T3‑E1 cell proliferation and differentiation were investigated. Cell counting kit‑8, reverse transcription‑polymerase chain reaction and biochemical methods, such as ELISA and alizarin red S staining, were used in the present study. It was revealed that mSGLXD not only stimulated MC3T3‑E1 cell proliferation, but also upregulated alkaline phosphatase and osteocalcin gene and protein expression levels. High concentrations of anastrozole (10 or 100 µmol/l) markedly inhibited MC3T3‑E1 cell proliferation, but this inhibitory effect was attenuated by mSGLXD. Furthermore, mSGLXD increased MC3T3‑E1 cell mineralization following β‑glycerophosphate and ascorbic acid induction. Therefore, the results of the present study suggested that mSGLXD may be a promising adjuvant therapy, with high safety and efficacy, for the prevention and treatment of AIBL in patients with breast cancer who receive AI treatment.
MeSH term(s)	Alkaline Phosphatase/genetics ; Alkaline Phosphatase/metabolism ; Anastrozole ; Animals ; Calcification, Physiologic/drug effects ; Cell Differentiation/drug effects ; Cell Line ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Drugs, Chinese Herbal/pharmacology ; Gene Expression ; Humans ; Mice ; Nitriles/pharmacology ; Osteoblasts/cytology ; Osteoblasts/drug effects ; Osteocalcin/genetics ; Osteocalcin/metabolism ; RNA, Messenger/genetics ; Triazoles/pharmacology
Chemical Substances	Drugs, Chinese Herbal ; Nitriles ; RNA, Messenger ; Triazoles ; shugan liangxue ; Osteocalcin (104982-03-8) ; Anastrozole (2Z07MYW1AZ) ; Alkaline Phosphatase (EC 3.1.3.1)
Language	English
Publishing date	2014-11-17
Publishing country	Greece
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2469505-1
ISSN	1791-3004 ; 1791-2997
ISSN (online)	1791-3004
ISSN	1791-2997
DOI	10.3892/mmr.2014.2962
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