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Article ; Online: Distinct presentation of melanoma in Black patients may inform strategies to improve outcomes.

Steadman, Jessica A / Glasgow, Amy E / Neequaye, Nikki N / Habermann, Elizabeth B / Hieken, Tina J

2024 Volume 129, Issue 6, Page(s) 1041–1050

Abstract: Introduction: Melanoma guidelines stem largely from data on non-Hispanic White (NHW) patients. We aimed to identify features of melanoma within non-Hispanic Black (NHB) patients to inform strategies for earlier detection and treatment.: Methods: From ...

Abstract	Introduction: Melanoma guidelines stem largely from data on non-Hispanic White (NHW) patients. We aimed to identify features of melanoma within non-Hispanic Black (NHB) patients to inform strategies for earlier detection and treatment. Methods: From 2004 to 2019 Surveillance, Epidemiology, and End Results (SEER) data, we identified nonmetastatic melanoma patients with known TN category and race. Kaplan-Meier cancer-specific survival (CSS) estimates and multivariable Cox proportional hazard modeling analyses were performed. Results: Of 492 597 patients, 1499 (0.3%) were NHB, who were younger (21% vs. 17% age <50) and more commonly female (54% vs. 41%) than NHW, both p < 0.0005. For NHBs, lower extremity was the most common site (52% vs. 15% for NHWs, p < 0.0001), T category was higher (55% Tis-T1 vs. 82%; 27% T3-T4 vs. 8%, p < 0.0001) and stage at presentation was higher (19% Stage III, vs. 6%, p < 0.0001). Within the NHB cohort, males were older, and more often node-positive than females. Five-year Stage III CSS was 42% for NHB males versus 71% for females, adjusting for age and clinical nodal status (hazard ratio 2.48). Conclusions: NHB melanoma patients presented with distinct tumor characteristics. NHB males with Stage III disease had inferior CSS. Focus on this high-risk patient cohort to promote earlier detection and treatment may improve outcomes.
MeSH term(s)	Humans ; Melanoma/pathology ; Melanoma/mortality ; Melanoma/therapy ; Melanoma/ethnology ; Male ; Female ; Middle Aged ; SEER Program ; Skin Neoplasms/pathology ; Skin Neoplasms/mortality ; Skin Neoplasms/therapy ; Skin Neoplasms/ethnology ; Survival Rate ; Black or African American/statistics & numerical data ; Aged ; Adult ; Prognosis ; Follow-Up Studies
Language	English
Publishing date	2024-03-04
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	82063-5
ISSN	1096-9098 ; 0022-4790
ISSN (online)	1096-9098
ISSN	0022-4790
DOI	10.1002/jso.27608
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Article ; Online: LiLA: lipid lung-based ATLAS built through a comprehensive workflow designed for an accurate lipid annotation.

Fernández Requena, Belén / Nadeem, Sajid / Reddy, Vineel P / Naidoo, Vanessa / Glasgow, Joel N / Steyn, Adrie J C / Barbas, Coral / Gonzalez-Riano, Carolina

Communications biology

2024 Volume 7, Issue 1, Page(s) 45

Abstract: Accurate lipid annotation is crucial for understanding the role of lipids in health and disease and identifying therapeutic targets. However, annotating the wide variety of lipid species in biological samples remains challenging in untargeted lipidomic ... ...

Abstract	Accurate lipid annotation is crucial for understanding the role of lipids in health and disease and identifying therapeutic targets. However, annotating the wide variety of lipid species in biological samples remains challenging in untargeted lipidomic studies. In this work, we present a lipid annotation workflow based on LC-MS and MS/MS strategies, the combination of four bioinformatic tools, and a decision tree to support the accurate annotation and semi-quantification of the lipid species present in lung tissue from control mice. The proposed workflow allowed us to generate a lipid lung-based ATLAS (LiLA), which was then employed to unveil the lipidomic signatures of the Mycobacterium tuberculosis infection at two different time points for a deeper understanding of the disease progression. This workflow, combined with manual inspection strategies of MS/MS data, can enhance the annotation process for lipidomic studies and guide the generation of sample-specific lipidome maps. LiLA serves as a freely available data resource that can be employed in future studies to address lipidomic alterations in mice lung tissue.
MeSH term(s)	Animals ; Mice ; Tandem Mass Spectrometry ; Workflow ; Ascomycota ; Computational Biology ; Lipids
Chemical Substances	Lipids
Language	English
Publishing date	2024-01-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-05680-7
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Article ; Online: A prospective bacterial whole-genome-sequencing-based surveillance programme for comprehensive early detection of healthcare-associated infection transmission in paediatric oncology patients.

Hakim, H / Glasgow, H L / Brazelton, J N / Gilliam, C H / Richards, L / Hayden, R T

The Journal of hospital infection

2023 Volume 143, Page(s) 53–63

Abstract: Background: Bacterial whole-genome sequencing (WGS) and determination of genetic relatedness is an important tool for investigation of epidemiologically suspected outbreaks.: Aim: This prospective cohort study evaluated a comprehensive, prospective ... ...

Abstract	Background: Bacterial whole-genome sequencing (WGS) and determination of genetic relatedness is an important tool for investigation of epidemiologically suspected outbreaks. Aim: This prospective cohort study evaluated a comprehensive, prospective bacterial WGS-based surveillance programme for early detection of transmission of most bacterial pathogens among patients at a paediatric oncology hospital. Methods: Cultured bacterial isolates from clinical diagnostic specimens collected prospectively from both inpatient and outpatient encounters between January 2019 and December 2021 underwent routine WGS and core genome multi-locus sequence typing to determine isolates' relatedness. Previously collected isolates from January to December 2018 were retrospectively analysed for identification of prior or ongoing transmission. Multi-patient clusters were investigated to identify potential transmission events based on temporal and spatial epidemiological links and interventions were introduced. Findings: A total of 1497 bacterial isolates from 1025 patients underwent WGS. A total of 259 genetically related clusters were detected, of which 18 (6.9%) multi-patient clusters involving 38 (3.7%) patients were identified. Sixteen clusters involved two patients each, and two clusters involved three patients. Following investigation, epidemiologically plausible transmission links were identified in five (27.8%) multi-patient clusters. None of the multi-patient clusters were suspected by conventional epidemiological surveillance. Conclusion: Bacterial WGS-based surveillance for early detection of hospital transmission detected several limited multi-patient clusters that were unrecognized by conventional epidemiological methods. Genomic surveillance helped efficiently focus interventions while reducing unnecessary investigations.
MeSH term(s)	Child ; Humans ; Multilocus Sequence Typing ; Prospective Studies ; Retrospective Studies ; Whole Genome Sequencing/methods ; Disease Outbreaks ; Cross Infection/microbiology ; Delivery of Health Care ; Genome, Bacterial
Language	English
Publishing date	2023-11-07
Publishing country	England
Document type	Journal Article
ZDB-ID	779366-2
ISSN	1532-2939 ; 0195-6701
ISSN (online)	1532-2939
ISSN	0195-6701
DOI	10.1016/j.jhin.2023.10.015
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Zs.A 1586: Show issues

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Article ; Online: Use of frailty assessment instruments in nephrology populations: a scoping review.

Kennard, Alice L / Rainsford, Suzanne / Glasgow, Nicholas J / Talaulikar, Girish S

BMC geriatrics

2023 Volume 23, Issue 1, Page(s) 449

Abstract: ... Studies of frailty in conservatively managed populations were infrequent (N = 4). We verified that frailty ...

Abstract	Background: Frailty is a clinical syndrome of accelerated aging associated with adverse outcomes. Frailty is prevalent among patients with chronic kidney disease but is infrequently assessed in clinical settings, due to lack of consensus regarding frailty definitions and diagnostic tools. This study aimed to review the practice of frailty assessment in nephrology populations and evaluate the context and timing of frailty assessment. Methods: The search included published reports of frailty assessment in patients with chronic kidney disease, undergoing dialysis or in receipt of a kidney transplant, published between January 2000 and November 2021. Medline, CINAHL, Embase, PsychINFO, PubMed and Cochrane Library databases were examined. A total of 164 articles were included for review. Results: We found that studies were most frequently set within developed nations. Overall, 161 studies were frailty assessments conducted as part of an observational study design, and 3 within an interventional study. Studies favoured assessment of participants with chronic kidney disease (CKD) and transplant candidates. A total of 40 different frailty metrics were used. The most frequently utilised tool was the Fried frailty phenotype. Frailty prevalence varied across populations and research settings from 2.8% among participants with CKD to 82% among patients undergoing haemodialysis. Studies of frailty in conservatively managed populations were infrequent (N = 4). We verified that frailty predicts higher rates of adverse patient outcomes. There is sufficient literature to justify future meta-analyses. Conclusions: There is increasing recognition of frailty in nephrology populations and the value of assessment in informing prognostication and decision-making during transitions in care. The Fried frailty phenotype is the most frequently utilised assessment, reflecting the feasibility of incorporating objective measures of frailty and vulnerability into nephrology clinical assessment. Further research examining frailty in low and middle income countries as well as first nations people is required. Future work should focus on interventional strategies exploring frailty rehabilitation.
MeSH term(s)	Humans ; Frailty/diagnosis ; Frailty/epidemiology ; Nephrology ; Aging ; Consensus ; Databases, Factual ; Observational Studies as Topic
Language	English
Publishing date	2023-07-21
Publishing country	England
Document type	Review ; Journal Article
ZDB-ID	2059865-8
ISSN	1471-2318 ; 1471-2318
ISSN (online)	1471-2318
ISSN	1471-2318
DOI	10.1186/s12877-023-04101-y
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Article ; Online: The mental health of Australians bereaved during the first two years of the COVID-19 pandemic: a latent class analysis.

Maccallum, F / Breen, L J / Phillips, J L / Agar, M R / Hosie, A / Tieman, J / DiGiacomo, M / Luckett, T / Philip, J / Ivynian, S / Chang, S / Dadich, A / Grossman, C H / Gilmore, I / Harlum, J / Kinchin, I / Glasgow, N / Lobb, E A

Psychological medicine

2024 Volume 54, Issue 7, Page(s) 1361–1372

Abstract: Background: The COVID-19 pandemic disrupted many areas of life, including culturally accepted practices at end-of-life care, funeral rites, and access to social, community, and professional support. This survey investigated the mental health outcomes of ...

Abstract	Background: The COVID-19 pandemic disrupted many areas of life, including culturally accepted practices at end-of-life care, funeral rites, and access to social, community, and professional support. This survey investigated the mental health outcomes of Australians bereaved during this time to determine how these factors might have impacted bereavement outcomes. Methods: An online survey indexing pandemic and bereavement experiences, levels of grief, depression, anxiety, and health, work, and social impairment. Latent class analysis (LCA) was used to identify groups of individuals who shared similar symptom patterns. Multinomial regressions identified pandemic-related, loss-related, and sociodemographic correlates of class membership. Results: 1911 Australian adults completed the survey. The LCA identified four classes: low symptoms (46.8%), grief (17.3%), depression/anxiety (17.7%), and grief/depression/anxiety (18.2%). The latter group reported the highest levels of health, work, and social impairment. The death of a child or partner and an inability to care for the deceased due to COVID-19 public health measures were correlated with grief symptoms (with or without depression and anxiety). Preparedness for the person's death and levels of pandemic-related loneliness and social isolation differentiated all four classes. Unemployment was associated with depression/anxiety (with or without grief). Conclusions: COVID-19 had profound impacts for the way we lived and died, with effects that are likely to ricochet through society into the foreseeable future. These lessons learned must inform policymakers and healthcare professionals to improve bereavement care and ensure preparedness during and following future predicted pandemics to prevent negative impacts.
MeSH term(s)	Adult ; Humans ; Australasian People ; Australia/epidemiology ; Bereavement ; COVID-19/psychology ; Grief ; Latent Class Analysis ; Mental Health ; Pandemics ; Stress Disorders, Post-Traumatic/psychology
Language	English
Publishing date	2024-01-05
Publishing country	England
Document type	Journal Article
ZDB-ID	217420-0
ISSN	1469-8978 ; 0033-2917
ISSN (online)	1469-8978
ISSN	0033-2917
DOI	10.1017/S0033291723003227
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Zs.B 1003: Show issues

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Article ; Online: An Analysis of Negative One-star Patient Reviews and Complaints for Pediatric Orthopaedic Surgeons throughout the United States: A Retrospective Study.

Hitchman, Kyle J / Baumann, Anthony N / Glasgow, Wyatt R / Rivera, Karla G / Anastasio, Albert T / Walley, Kempland C / Baldwin, Keith D

Journal of pediatric orthopedics

2023 Volume 44, Issue 2, Page(s) 129–134

Abstract: Objective: Patients commonly use physician review websites when choosing a surgeon for an elective procedure. Although data exist regarding other orthopaedic specialties, no study has investigated one-star reviews for pediatric orthopaedic surgeons. The ...

Abstract	Objective: Patients commonly use physician review websites when choosing a surgeon for an elective procedure. Although data exist regarding other orthopaedic specialties, no study has investigated one-star reviews for pediatric orthopaedic surgeons. The goal of this retrospective study was to classify the factors contributing to one-star reviews of pediatric orthopaedic surgeons to identify which areas contribute to lower patient satisfaction. Methods: Patient ratings on a 5-star system and comments about pediatric orthopaedic surgeons were collected from the state with the most physicians registered in the "Pediatric Orthopaedic Society of North American" database for each of the 9 geographical regions of the United States as defined by the Association of American Medical Colleges. One-star reviews that included comments were classified as either surgical or nonsurgical. These comments were then further classified based on their content. Results: Three hundred fifty-four one-star reviews with 700 complaints were included in this study. Of these complaints, 481 (68.7%) were from nonsurgical patients and 219 (31.3%) were from surgical patients. Nonsurgical patients were significantly more likely to reference the amount of time spent with the physician (12.9% to 6.6%, P = 0.026), wait time (11.9% to 0.0%, P < 0.001), and bedside manner (41.2% to 22.8%, P < 0.001). Patients who said they had undergone a surgical procedure in their one-star review were significantly more likely to reference a disagreement with the physician's decision or plan (35.3% to 17.5%, P < 0.001), and uncontrolled pain (21.6% to 5.2%, P < 0.001). There was no significant difference in the comments that referenced medical staff or institutional complaints between surgical and nonsurgical patients (13.8% to 11.4%, P = 0.424). Conclusion: Most one-star reviews of pediatric orthopaedic surgeons referenced interpersonal skills and other nonclinical aspects of a clinical encounter, with bedside manner being the most frequent complaint. Patients who had undergone surgical procedures were less likely to leave a one-star review, but if they did, their comment was usually about a disagreement with the physician's plan. Clinical relevance: Prognostic studies III.
MeSH term(s)	Humans ; United States ; Child ; Orthopedic Surgeons ; Retrospective Studies ; Orthopedics ; Surgeons ; Patient Satisfaction
Language	English
Publishing date	2023-11-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	604642-3
ISSN	1539-2570 ; 0271-6798
ISSN (online)	1539-2570
ISSN	0271-6798
DOI	10.1097/BPO.0000000000002571
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Article: Methods toward simplification of time resolved fluorescence anisotropy in proteins labeled with NBD (4-chloro-7-nitrobenzofurazan) adducts.

Glasgow, Ben J / Abduragimov, Adil R

MethodsX

2019 Volume 6, Page(s) 998–1008

Abstract: ... were taken toward simplification. First, N terminal selective labeling of tear lipocalin with NBD-Cl ... was attempted at pH 7.0. While lysines were predominantly labeled at pH 8.0, selective N terminal ... correlated photon counting. •NBD labeling at pH 7.0 was not selective for N terminus of LCN1.•Constraints ...

Abstract	The analysis of time resolved fluorescence anisotropy for NBD tagged proteins is difficult when multiple exponential components arise from heterogeneous amino acid fluorescent adducts. Two approaches were taken toward simplification. First, N terminal selective labeling of tear lipocalin with NBD-Cl was attempted at pH 7.0. While lysines were predominantly labeled at pH 8.0, selective N terminal labeling was attained at neutral pH. Second, fluorescence anisotropic decay analysis was simplified to recover only the rotational correlation time of the protein not the side chain. The boundaries for analysis of anisotropic decays were limited to the longer lifetimes. A modified tail fit enabled fitting the anisotropic decay to a single exponential. The correlation time for tear lipocalin matched published values. Additionally, a method for normalization of acquisition times of vertically (VV) and horizontally (VH) polarized fluorescence emission decays is presented for time-resolved anisotropy. Here it is applied to Picoharp software (Picoquant, Berlin). Picoharp software is programmed with an automatic stop at unequal acquisition times if the fluorescent counts exceeds a default. The method adjusts the intensity decays to the same acquisition time and is applicable to all time-resolved anisotropic decay data collected with time-correlated photon counting. •NBD labeling at pH 7.0 was not selective for N terminus of LCN1.•Constraints for range simplifies fittings of anisotropic decays.•Different acquisition times for decays can be normalized to facilitate fitting in data obtained by Picoharp.
Language	English
Publishing date	2019-04-29
Publishing country	Netherlands
Document type	Journal Article
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2019.04.025
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Article ; Online: Low-grade fibromyxoid sarcoma of the orbit.

Oh, Angela J / Singh, Pallavi / Pirakitikulr, Nathan / Roelofs, Kelsey / Glasgow, Ben J / Rootman, Daniel B

Orbit (Amsterdam, Netherlands)

2022 , Page(s) 1–5

Abstract: A 70-year-old male presented with diplopia and painless proptosis of the left eye for 5 months. Examination showed 6 mm of axial proptosis and restriction of supraduction, abduction and adduction, and mild limitation of infraduction of the left eye. ... ...

Abstract	A 70-year-old male presented with diplopia and painless proptosis of the left eye for 5 months. Examination showed 6 mm of axial proptosis and restriction of supraduction, abduction and adduction, and mild limitation of infraduction of the left eye. Magnetic resonance imaging demonstrated a large, moderately well-circumscribed intraconal mass in the left lateral orbit, and excisional biopsy was performed. Histopathologic features of mixed fibrous and myxoid areas in a whorl-like pattern and immunohistochemical staining for MUC4 confirmed the diagnosis of low-grade fibromyxoid sarcoma (LGFMS). Next-generation sequencing revealed genetic fusion of EWSR1-CREB3L1. LGFMS is an extremely rare neoplasm with only two prior documented cases of orbital involvement. Here, we report the third case of orbital LGFMS.
Language	English
Publishing date	2022-11-28
Publishing country	England
Document type	Journal Article
ZDB-ID	603157-2
ISSN	1744-5108 ; 0167-6830
ISSN (online)	1744-5108
ISSN	0167-6830
DOI	10.1080/01676830.2022.2149820
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Zs.A 1735: Show issues

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Article ; Online: Microenvironment Regulators of Metastasis Favor Lymphangioleiomyomatosis Cell Growth and Disease Progression.

Pacheco-Rodriguez, Gustavo / Steagall, Wendy K / Glasgow, Connie G / Onishi, Rieko / Stylianou, Mario / Kato, Jiro / Li, Shaowei / Samsel, Leigh / McCoy, J Philip / Darling, Thomas N / Moss, Joel

American journal of respiratory cell and molecular biology

2023 Volume 68, Issue 5, Page(s) 591–594

MeSH term(s)	Humans ; Lymphangioleiomyomatosis/pathology ; Lung Neoplasms/pathology ; Cell Proliferation ; Disease Progression ; Tumor Microenvironment
Language	English
Publishing date	2023-04-26
Publishing country	United States
Document type	Letter ; Research Support, N.I.H., Intramural
ZDB-ID	1025960-0
ISSN	1535-4989 ; 1044-1549
ISSN (online)	1535-4989
ISSN	1044-1549
DOI	10.1165/rcmb.2022-0419LE
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Article ; Online: Establishing Riboglow-FLIM to visualize noncoding RNAs inside live zebrafish embryos.

Sarfraz, Nadia / Lee, Harrison J / Rice, Morgan K / Moscoso, Emilia / Shafik, Luke K / Glasgow, Eric / Ranjit, Suman / Lambeck, Ben J / Braselmann, Esther

Biophysical reports

2023 Volume 3, Issue 4, Page(s) 100132

Abstract: The central role of RNAs in health and disease calls for robust tools to visualize RNAs in living systems through fluorescence microscopy. Live zebrafish embryos are a popular system to investigate multicellular complexity as disease models. However, RNA ...

Abstract	The central role of RNAs in health and disease calls for robust tools to visualize RNAs in living systems through fluorescence microscopy. Live zebrafish embryos are a popular system to investigate multicellular complexity as disease models. However, RNA visualization approaches in whole organisms are notably underdeveloped. Here, we establish our RNA tagging and imaging platform Riboglow-FLIM for complex cellular imaging applications by systematically evaluating FLIM capabilities. We use adherent mammalian cells as models for RNA visualization. Additional complexity of analyzing RNAs in whole mammalian animals is achieved by injecting these cells into a zebrafish embryo system for cell-by-cell analysis in this model of multicellularity. We first evaluate all variable elements of Riboglow-FLIM quantitatively before assessing optimal use in whole animals. In this way, we demonstrate that a model noncoding RNA can be detected robustly and quantitatively inside live zebrafish embryos using a far-red Cy5-based variant of the Riboglow platform. We can clearly resolve cell-to-cell heterogeneity of different RNA populations by this methodology, promising applicability in diverse fields.
Language	English
Publishing date	2023-09-26
Publishing country	United States
Document type	Journal Article
ISSN	2667-0747
ISSN (online)	2667-0747
DOI	10.1016/j.bpr.2023.100132
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