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  1. Book ; Thesis: Isolierung und Charakterisierung von Staufen- und Barentsz-enthaltenden Ribonukleoproteinpartikeln aus Rattenhirn

    Karra, Daniela

    2008  

    Author's details von Daniela Karra
    Subject code 573.8648419352
    Language German
    Size V, 146 S., Ill., graph. Darst.
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Tübingen, Univ., Diss., 2008 (Nur beschränkt für den Austausch)
    HBZ-ID HT015717584
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2009 D 540 order for loan Magazin

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  2. Book ; Online ; Thesis: Isolierung und Charakterisierung von Staufen- und Barentsz- enthaltenden Ribonukleoproteinpartikeln aus Rattenhirn

    Karra, Daniela

    2010  

    Author's details Daniela Karra
    Language German
    Size Online-Ressource
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Univ., Diss.--Tübingen, 2008
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  3. Article ; Online: Transfection techniques for neuronal cells.

    Karra, Daniela / Dahm, Ralf

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2010  Volume 30, Issue 18, Page(s) 6171–6177

    MeSH term(s) Animals ; Biolistics/methods ; Electroporation/methods ; Humans ; Neurons/physiology ; Transduction, Genetic/methods ; Transfection/methods
    Language English
    Publishing date 2010-05-05
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.0183-10.2010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1668: Show issues Location:
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  4. Book ; Online ; Thesis: Isolierung und Charakterisierung von Staufen- und Barentsz-enthaltenden Ribonukleoproteinpartikeln aus Rattenhirn

    Karra, Daniela [Verfasser]

    2008  

    Author's details von Daniela Karra
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language German
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

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  5. Article: More than just synaptic building blocks: scaffolding proteins of the post-synaptic density regulate dendritic patterning.

    Vessey, John P / Karra, Daniela

    Journal of neurochemistry

    2007  Volume 102, Issue 2, Page(s) 324–332

    Abstract: The dendritic arbor is responsible for receiving and consolidating neuronal input. Outgrowth and morphogenesis of the arbor are complex stages of development that are poorly understood. However, recent findings have identified synaptic scaffolding ... ...

    Abstract The dendritic arbor is responsible for receiving and consolidating neuronal input. Outgrowth and morphogenesis of the arbor are complex stages of development that are poorly understood. However, recent findings have identified synaptic scaffolding proteins as novel regulators of these important events. Scaffolding proteins are enriched in the post-synaptic density where they bind and bring into close proximity neurotransmitter receptors, signaling molecules, and regulators of the actin cytoskeleton. This property is important for dendritic spine morphogenesis and maintenance in the mature neuron. Scaffolding proteins are now being described as key regulators of neurite outgrowth, dendritic development, and pattern formation in immature neurons. These proteins, which include post-synaptic-95, Shank and Densin-180, as well as many of their interacting partners, appear to regulate both the microtubule and actin cytoskeleton to influence dendrite morphology. Through a large array of protein-protein interaction domains, scaffolding proteins are able to form large macromolecular complexes that include cytoskeletal motor proteins as well as microtubule and actin regulatory molecules. Together, the new findings form a persuasive argument that scaffolding proteins deliver critical regulatory elements to sites of dendritic outgrowth and branching to modulate the formation and maintenance of the dendritic arbor.
    MeSH term(s) Animals ; Cell Differentiation/physiology ; Cell Shape/physiology ; Central Nervous System/embryology ; Central Nervous System/metabolism ; Central Nervous System/ultrastructure ; Cytoskeleton/metabolism ; Dendrites/metabolism ; Dendrites/ultrastructure ; Disks Large Homolog 4 Protein ; Humans ; Intracellular Signaling Peptides and Proteins/metabolism ; Membrane Proteins/metabolism ; Molecular Motor Proteins/metabolism ; Synaptic Membranes/metabolism ; Synaptic Membranes/ultrastructure
    Chemical Substances Disks Large Homolog 4 Protein ; Dlg4 protein, rat ; Intracellular Signaling Peptides and Proteins ; Membrane Proteins ; Molecular Motor Proteins
    Language English
    Publishing date 2007-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 80158-6
    ISSN 1471-4159 ; 0022-3042 ; 1474-1644
    ISSN (online) 1471-4159
    ISSN 0022-3042 ; 1474-1644
    DOI 10.1111/j.1471-4159.2007.04662.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Ui II Zs.170: Show issues Location:
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  6. Conference proceedings: MicroRNA-134 shows reduced expression in medulloblastoma and regulates the expression of NMYC

    Zipper, Petra / Karra, Daniela / Bolat, Özlem / Reifenberger, Guido

    2013  , Page(s) P 149

    Event/congress 64. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC); Düsseldorf; Deutsche Gesellschaft für Neurochirurgie; 2013
    Keywords Medizin, Gesundheit ; miR-134 ; medulloblastoma ; NMYC
    Publishing date 2013-05-21
    Publisher German Medical Science GMS Publishing House; Düsseldorf
    Document type Conference proceedings
    DOI 10.3205/13dgnc566
    Database German Medical Science

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  7. Article: More than just synaptic building blocks: scaffolding proteins of the post-synaptic density regulate dendritic patterning

    Vessey, John P / Karra, Daniela

    Journal of neurochemistry. 2007 July, v. 102, no. 2

    2007  

    Abstract: The dendritic arbor is responsible for receiving and consolidating neuronal input. Outgrowth and morphogenesis of the arbor are complex stages of development that are poorly understood. However, recent findings have identified synaptic scaffolding ... ...

    Abstract The dendritic arbor is responsible for receiving and consolidating neuronal input. Outgrowth and morphogenesis of the arbor are complex stages of development that are poorly understood. However, recent findings have identified synaptic scaffolding proteins as novel regulators of these important events. Scaffolding proteins are enriched in the post-synaptic density where they bind and bring into close proximity neurotransmitter receptors, signaling molecules, and regulators of the actin cytoskeleton. This property is important for dendritic spine morphogenesis and maintenance in the mature neuron. Scaffolding proteins are now being described as key regulators of neurite outgrowth, dendritic development, and pattern formation in immature neurons. These proteins, which include post-synaptic-95, Shank and Densin-180, as well as many of their interacting partners, appear to regulate both the microtubule and actin cytoskeleton to influence dendrite morphology. Through a large array of protein-protein interaction domains, scaffolding proteins are able to form large macromolecular complexes that include cytoskeletal motor proteins as well as microtubule and actin regulatory molecules. Together, the new findings form a persuasive argument that scaffolding proteins deliver critical regulatory elements to sites of dendritic outgrowth and branching to modulate the formation and maintenance of the dendritic arbor.
    Language English
    Dates of publication 2007-07
    Size p. 324-332.
    Publisher Blackwell Publishing Ltd
    Publishing place Oxford, UK
    Document type Article
    ZDB-ID 80158-6
    ISSN 1471-4159 ; 0022-3042 ; 1474-1644
    ISSN (online) 1471-4159
    ISSN 0022-3042 ; 1474-1644
    DOI 10.1111/j.1471-4159.2007.04662.x
    Database NAL-Catalogue (AGRICOLA)

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  8. Conference proceedings: MicroRNA-132 frequently shows reduced expression in medulloblastoma and regulates the expression of Sirt1

    Zipper, Petra / Liesenberg, Franziska / Forchmann, Anneliese / Karra, Daniela / Bolat, Özlem / Reifenberger, Guido

    2012  , Page(s) 12dgnnOP14

    Event/congress 57th Annual Meeting of the German Society for Neuropathology and Neuroanatomy (DGNN); Erlangen; Deutsche Gesellschaft für Neuropathologie und Neuroanatomie; 2012
    Keywords Medizin, Gesundheit
    Publishing date 2012-09-11
    Publisher German Medical Science GMS Publishing House; Düsseldorf
    Document type Conference proceedings
    DOI 10.3205/12dgnn014
    Database German Medical Science

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  9. Article: Population haplotypes of exon ORF15 of the retinitis pigmentosa GTPase regulator gene in Germany : implications for screening for inherited retinal disorders.

    Karra, Daniela / Jacobi, Felix K / Broghammer, Martina / Blin, Nikolaus / Pusch, Carsten M

    Molecular diagnosis & therapy

    2006  Volume 10, Issue 2, Page(s) 115–123

    Abstract: Background: Mutations in exon ORF15 of the retinitis pigmentosa GTPase regulator gene (RPGR) within chromosomal region Xp21.1 are a significant cause of a number of retinal disorders. The high mutation rate is ascribed to the highly repetitive, purine- ... ...

    Abstract Background: Mutations in exon ORF15 of the retinitis pigmentosa GTPase regulator gene (RPGR) within chromosomal region Xp21.1 are a significant cause of a number of retinal disorders. The high mutation rate is ascribed to the highly repetitive, purine-rich tracts within the exon ORF15 sequence. Importantly, all exon ORF15 mutations observed to date represent protein-truncating mutations (nonsense and frameshift mutations). Because of its repetitive motifs, mutation screening of the hot-spot region by direct DNA sequencing is a technically challenging task.
    Methods: We devised a screening strategy for exon ORF15 mutations that reserves DNA sequencing for precise sizing and base-order assessment of detected mutations. The screening strategy is based on a PCR/restriction fragment length polymorphism (RFLP) analysis of exon ORF15 and comparison with population-specific RFLP haplotypes. The latter were constructed from PCR/RFLP analysis of DNA samples from 100 healthy German male individuals. Mutational alterations of normal RFLP haplotype patterns were predicted.
    Results: Six distinct RFLP haplotypes (founder alleles H1-H6) were observed with frequencies ranging from 2% to 63%. All natural variations of exon ORF15 were in-frame alterations ranging in size between 3bp and 36bp. Prediction of mutation-specific RFLP patterns indicated a high detection rate of mutations.
    Conclusion: A new strategy has been developed using routine protocols for mutation screening of difficult-to-sequence, highly repetitive exon ORF15 of the RPGR gene in a German population.
    MeSH term(s) Base Sequence ; Chromosomes, Human, Pair 21/genetics ; DNA Mutational Analysis/methods ; Eye Proteins/genetics ; Genetic Testing/methods ; Germany ; Haplotypes ; Humans ; Male ; Molecular Sequence Data ; Mutation ; Open Reading Frames/genetics ; Polymorphism, Restriction Fragment Length ; Population/genetics ; Retinal Diseases/genetics
    Chemical Substances Eye Proteins ; RPGR protein, human
    Language English
    Publishing date 2006-04-28
    Publishing country New Zealand
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2232796-4
    ISSN 1179-2000 ; 1177-1062
    ISSN (online) 1179-2000
    ISSN 1177-1062
    DOI 10.1007/BF03256451
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 5202: Show issues Location:
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  10. Article ; Online: High-efficiency transfection of short hairpin RNAs-encoding plasmids into primary hippocampal neurons.

    Zeitelhofer, Manuel / Karra, Daniela / Vessey, John P / Jaskic, Elmir / Macchi, Paolo / Thomas, Sabine / Riefler, Julia / Kiebler, Michael / Dahm, Ralf

    Journal of neuroscience research

    2009  Volume 87, Issue 1, Page(s) 289–300

    Abstract: The transfection of expression constructs encoding a variety of transgenes is a widely used method to study gene function in cultured cells. Especially when the efficiency of the knock-down of target proteins via small interfering RNAs (siRNAs) is to be ... ...

    Abstract The transfection of expression constructs encoding a variety of transgenes is a widely used method to study gene function in cultured cells. Especially when the efficiency of the knock-down of target proteins via small interfering RNAs (siRNAs) is to be determined by quantitative Western blotting, large proportions of untransfected cells compromise the analysis. Achieving high transfection efficiencies in postmitotic cells, such as neurons, poses a particular problem in that these cells cannot be selected for the expression of the transgene following transfection. It is therefore important to develop protocols that allow for the highly efficient transfection of these cells. In the present study, we identify three important parameters that prove especially useful for chronically difficult to transfect short hairpin RNA (shRNA)-encoding plasmids: the amount and quality of the plasmid DNA used and the use of new nucleofection programs. Combining those changes increases the rate of transfected cells from less than 5% to up to approximately 80%. Importantly, these high transfection efficiencies can be obtained while maintaining good cell viability and normal cellular development. Taken together, these improvements allow for a detailed biochemical and phenotypical analysis of neurons that have been nucleoporated with a wide variety of shRNAs.
    MeSH term(s) Animals ; Cells, Cultured ; Embryo, Mammalian ; Genetic Techniques ; Green Fluorescent Proteins/genetics ; Hippocampus/cytology ; Neurons/physiology ; Plasmids/genetics ; RNA/chemistry ; RNA/genetics ; RNA Interference/physiology ; Rats ; Transfection/methods
    Chemical Substances Green Fluorescent Proteins (147336-22-9) ; RNA (63231-63-0)
    Language English
    Publishing date 2009-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 195324-2
    ISSN 1097-4547 ; 0360-4012
    ISSN (online) 1097-4547
    ISSN 0360-4012
    DOI 10.1002/jnr.21840
    Database MEDical Literature Analysis and Retrieval System OnLINE

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