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Article ; Online: Care in crisis

Elias, Nicole M. / D’Agostino, Maria J.

COVID-19 as a catalyst for universal child care in the United States

2020 , Page(s) 1–13

Keywords	covid19
Language	English
Publisher	Informa UK Limited
Publishing country	uk
Document type	Article ; Online
ZDB-ID	2115175-1
ISSN	1949-0461 ; 1084-1806
ISSN (online)	1949-0461
ISSN	1084-1806
DOI	10.1080/10841806.2020.1813456
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Payment and Financing for Substance Use Screening and Brief Intervention for Adolescents and Adults in Health, School, and Community Settings.

Reif, Sharon / Brolin, Mary / Beyene, Tiginesh M / D'Agostino, Nicole / Stewart, Maureen T / Horgan, Constance M

The Journal of adolescent health : official publication of the Society for Adolescent Medicine

2022 Volume 71, Issue 4S, Page(s) S73–S82

Abstract: Screening and brief intervention (SBI) is an evidence-based, cost-effective practice to address unhealthy substance use. With SBI services expanding beyond healthcare settings (e.g., schools, community organizations) and reaching younger populations, ... ...

Abstract	Screening and brief intervention (SBI) is an evidence-based, cost-effective practice to address unhealthy substance use. With SBI services expanding beyond healthcare settings (e.g., schools, community organizations) and reaching younger populations, sustainability efforts must consider payment and financing. This narrative review incorporated rapid scoping review methods and a search of the gray literature to determine payment and financing approaches for SBI with adolescents and to describe related barriers and facilitators for its sustainability. We sought information relevant to adolescents and settings in which they receive SBI, but also reviewed sources with an adult focus. Few peer-reviewed articles met inclusion criteria, and those mostly highlighted healthcare settings. School-based settings were better described in the gray literature; little was found about community settings. SBI is mostly paid through grant funding and public and commercial insurance; school-based settings use a range of approaches including grants, public insurance, and other public funding. We call upon researchers and providers to describe the payment and financing of SBI, to inform how the uptake of SBI may be practicable and sustainable. The increasing activation and use of insurance billing codes, and the expansion of SBI beyond healthcare, is encouraging to address unhealthy substance use by adolescents.
MeSH term(s)	Adolescent ; Adult ; Crisis Intervention ; Humans ; Mass Screening/methods ; Research ; Schools ; Substance-Related Disorders/diagnosis ; Substance-Related Disorders/therapy
Language	English
Publishing date	2022-09-19
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	1063374-1
ISSN	1879-1972 ; 1054-139X
ISSN (online)	1879-1972
ISSN	1054-139X
DOI	10.1016/j.jadohealth.2022.04.012
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Article ; Online: Exogenous ketone ester delays CNS oxygen toxicity without impairing cognitive and motor performance in male Sprague-Dawley rats.

Stavitzski, Nicole M / Landon, Carol S / Hinojo, Christopher M / Poff, Angela M / Rogers, Christopher Q / D'Agostino, Dominic P / Dean, Jay B

American journal of physiology. Regulatory, integrative and comparative physiology

2021 Volume 321, Issue 2, Page(s) R100–R111

Abstract: Hyperbaric oxygen ( ... ...

Abstract	Hyperbaric oxygen (HBO
MeSH term(s)	Animals ; Anticonvulsants/pharmacokinetics ; Anticonvulsants/pharmacology ; Anticonvulsants/toxicity ; Behavior, Animal/drug effects ; Brain/drug effects ; Brain/physiopathology ; Cognition/drug effects ; Disease Models, Animal ; Esters/pharmacokinetics ; Esters/pharmacology ; Esters/toxicity ; Hyperbaric Oxygenation/adverse effects ; Ketones/pharmacokinetics ; Ketones/pharmacology ; Ketones/toxicity ; Male ; Motor Activity/drug effects ; Rats, Sprague-Dawley ; Reaction Time ; Seizures/etiology ; Seizures/physiopathology ; Seizures/prevention & control ; Seizures/psychology ; Rats
Chemical Substances	Anticonvulsants ; Esters ; Ketones
Language	English
Publishing date	2021-06-16
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	603839-6
ISSN	1522-1490 ; 0363-6119
ISSN (online)	1522-1490
ISSN	0363-6119
DOI	10.1152/ajpregu.00088.2021
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Article: Care in crisis: COVID-19 as a catalyst for universal child care in the United States

Elias, Nicole M. / D’Agostino, Maria J.

Adm. Theory Prax.

Abstract: School closings during COVID-19 exposed an under-addressed gender equity issue in the United States: child care in crisis. To better understand the child care crisis in the current U.S. context, we detail how New York City is addressing child care during ...

Abstract	School closings during COVID-19 exposed an under-addressed gender equity issue in the United States: child care in crisis. To better understand the child care crisis in the current U.S. context, we detail how New York City is addressing child care during COVID-19. We then connect the current approaches to the Lanham Act that was instituted during WWII as a historical parallel. Ultimately, we argue for the adoption of a universal system that is affordable, high-quality, federally-funded with local involvement and discretion, and flexible for primary caregivers seeking care support. This potential system builds on current congressional proposals and should take into account the challenges primary caregivers face in order to disrupt gender imbalances in care, and in turn, produce greater gender equity. COVID-19 is an opportunity to instill lasting change by improving the current U.S. child care model.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #735634
Database	COVID19

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Article ; Online: You & Me: Test and Treat study protocol for promoting COVID-19 test and treatment access to underserved populations.

D'Agostino, Emily M / Rosenberg, Lauren M / Richmond, Alan / Damman, Allyn / Brown-Lowery, Camille / Abbot-Grimes, Princess / Siddiqui, Saira / Fadika, Tigidankay / Ward, Mark / Cooper, Mia / Sutton, Sonya / Kenton, Lindsay / Spaziano, Bob / Kasper, Janet / Barnes, Nicole / Hornik, Christoph

BMC public health

2023 Volume 23, Issue 1, Page(s) 2121

Abstract: Background: Infections and deaths from the COVID-19 pandemic have disproportionately affected underserved populations. A community-engaged approach that supports decision making around safe COVID-19 practices is needed to promote equitable access to ... ...

Abstract	Background: Infections and deaths from the COVID-19 pandemic have disproportionately affected underserved populations. A community-engaged approach that supports decision making around safe COVID-19 practices is needed to promote equitable access to testing and treatment. You & Me: Test and Treat (YMTT) will evaluate a systematic and scalable community-engaged protocol that provides rapid access to COVID-19 at-home tests, education, guidance on next steps, and information on local resources to facilitate treatment in underserved populations. Methods: This direct-to-participant observational study will distribute at-home, self-administered, COVID-19 testing kits to people in designated communities. YMTT features a Public Health 3.0 framework and Toolkit prescribing a tiered approach to community engagement. We will partner with two large community organizations, Merced County United Way (Merced County, CA) and Pitt County Health Department (Pitt County, NC), who will coordinate up to 20 local partners to distribute 40,000 COVID tests and support enrollment, consenting, and data collection over a 15-month period. Participants will complete baseline questions about their demographics, experience with COVID-19 infection, and satisfaction with the distribution event. Community partners will also complete engagement surveys. In addition, participants will receive guidance on COVID-19 mitigation and health-promoting resources, and accessible and affordable therapeutics if they test positive for COVID-19. Data collection will be completed using a web-based platform that enables creation and management of electronic data capture forms. Implementation measures include evaluating 1) the Toolkit as a method to form community-academic partnerships for COVID-19 test access, 2) testing results, and 3) the efficacy of a YMTT protocol coupled with local resourcing to provide information on testing, guidance, treatment, and links to resources. Findings will be used to inform innovative methods to address community needs in public health research that foster cultural relevance, improve research quality, and promote health equity. Discussion: This work will promote access to COVID-19 testing and treatment for underserved populations by leveraging a community-engaged research toolkit. Future dissemination of the toolkit can support effective community-academic partnerships for health interventions in underserved settings. Trial registration: ClinicalTrials.gov Identifier: NCT05455190 . Registered 13 July 2022.
MeSH term(s)	Humans ; COVID-19/diagnosis ; Health Promotion ; COVID-19 Testing ; Vulnerable Populations ; Pandemics/prevention & control ; Community Participation ; Stakeholder Participation ; Observational Studies as Topic
Language	English
Publishing date	2023-10-28
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2041338-5
ISSN	1471-2458 ; 1471-2458
ISSN (online)	1471-2458
ISSN	1471-2458
DOI	10.1186/s12889-023-16960-6
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Article ; Online: You & Me

Emily M. D’Agostino / Lauren M. Rosenberg / Alan Richmond / Allyn Damman / Camille Brown-Lowery / Princess Abbot-Grimes / Saira Siddiqui / Tigidankay Fadika / Mark Ward / Mia Cooper / Sonya Sutton / Lindsay Kenton / Bob Spaziano / Janet Kasper / Nicole Barnes / Christoph Hornik

BMC Public Health, Vol 23, Iss 1, Pp 1-

Test and Treat study protocol for promoting COVID-19 test and treatment access to underserved populations

2023 Volume 11

Abstract: Abstract Background Infections and deaths from the COVID-19 pandemic have disproportionately affected underserved populations. A community-engaged approach that supports decision making around safe COVID-19 practices is needed to promote equitable access ...

Abstract	Abstract Background Infections and deaths from the COVID-19 pandemic have disproportionately affected underserved populations. A community-engaged approach that supports decision making around safe COVID-19 practices is needed to promote equitable access to testing and treatment. You & Me: Test and Treat (YMTT) will evaluate a systematic and scalable community-engaged protocol that provides rapid access to COVID-19 at-home tests, education, guidance on next steps, and information on local resources to facilitate treatment in underserved populations. Methods This direct-to-participant observational study will distribute at-home, self-administered, COVID-19 testing kits to people in designated communities. YMTT features a Public Health 3.0 framework and Toolkit prescribing a tiered approach to community engagement. We will partner with two large community organizations, Merced County United Way (Merced County, CA) and Pitt County Health Department (Pitt County, NC), who will coordinate up to 20 local partners to distribute 40,000 COVID tests and support enrollment, consenting, and data collection over a 15-month period. Participants will complete baseline questions about their demographics, experience with COVID-19 infection, and satisfaction with the distribution event. Community partners will also complete engagement surveys. In addition, participants will receive guidance on COVID-19 mitigation and health-promoting resources, and accessible and affordable therapeutics if they test positive for COVID-19. Data collection will be completed using a web-based platform that enables creation and management of electronic data capture forms. Implementation measures include evaluating 1) the Toolkit as a method to form community-academic partnerships for COVID-19 test access, 2) testing results, and 3) the efficacy of a YMTT protocol coupled with local resourcing to provide information on testing, guidance, treatment, and links to resources. Findings will be used to inform innovative methods to address community ...
Keywords	Community partners ; COVID-19 ; Guidance ; Health equity ; Protocol ; Public health ; Public aspects of medicine ; RA1-1270
Subject code	306
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Antarctic desert soil bacteria exhibit high novel natural product potential, evaluated through long-read genome sequencing and comparative genomics.

Benaud, Nicole / Edwards, Richard J / Amos, Timothy G / D'Agostino, Paul M / Gutiérrez-Chávez, Carolina / Montgomery, Kate / Nicetic, Iskra / Ferrari, Belinda C

Environmental microbiology

2020 Volume 23, Issue 7, Page(s) 3646–3664

Abstract: Actinobacteria and Proteobacteria are important producers of bioactive natural products (NP), and these phyla dominate in the arid soils of Antarctica, where metabolic adaptations influence survival under harsh conditions. Biosynthetic gene clusters ( ... ...

Abstract	Actinobacteria and Proteobacteria are important producers of bioactive natural products (NP), and these phyla dominate in the arid soils of Antarctica, where metabolic adaptations influence survival under harsh conditions. Biosynthetic gene clusters (BGCs) which encode NPs, are typically long and repetitious high G + C regions difficult to sequence with short-read technologies. We sequenced 17 Antarctic soil bacteria from multi-genome libraries, employing the long-read PacBio platform, to optimize capture of BGCs and to facilitate a comprehensive analysis of their NP capacity. We report 13 complete bacterial genomes of high quality and contiguity, representing 10 different cold-adapted genera including novel species. Antarctic BGCs exhibited low similarity to known compound BGCs (av. 31%), with an abundance of terpene, non-ribosomal peptide and polyketide-encoding clusters. Comparative genome analysis was used to map BGC variation between closely related strains from geographically distant environments. Results showed the greatest biosynthetic differences to be in a psychrotolerant Streptomyces strain, as well as a rare Actinobacteria genus, Kribbella, while two other Streptomyces spp. were surprisingly similar to known genomes. Streptomyces and Kribbella BGCs were predicted to encode antitumour, antifungal, antibacterial and biosurfactant-like compounds, and the synthesis of NPs with antibacterial, antifungal and surfactant properties was confirmed through bioactivity assays.
MeSH term(s)	Antarctic Regions ; Biological Products ; Genomics ; Phylogeny ; Soil ; Streptomyces
Chemical Substances	Biological Products ; Soil
Language	English
Publishing date	2020-11-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.15300
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Targeting the MR1-MAIT cell axis improves vaccine efficacy and affords protection against viral pathogens.

Rashu, Rasheduzzaman / Ninkov, Marina / Wardell, Christine M / Benoit, Jenna M / Wang, Nicole I / Meilleur, Courtney E / D'Agostino, Michael R / Zhang, Ali / Feng, Emily / Saeedian, Nasrin / Bell, Gillian I / Vahedi, Fatemeh / Hess, David A / Barr, Stephen D / Troyer, Ryan M / Kang, Chil-Yong / Ashkar, Ali A / Miller, Matthew S / Haeryfar, S M Mansour

PLoS pathogens

2023 Volume 19, Issue 6, Page(s) e1011485

Abstract: Mucosa-associated invariant T (MAIT) cells are MR1-restricted, innate-like T lymphocytes with tremendous antibacterial and immunomodulatory functions. Additionally, MAIT cells sense and respond to viral infections in an MR1-independent fashion. However, ... ...

Abstract	Mucosa-associated invariant T (MAIT) cells are MR1-restricted, innate-like T lymphocytes with tremendous antibacterial and immunomodulatory functions. Additionally, MAIT cells sense and respond to viral infections in an MR1-independent fashion. However, whether they can be directly targeted in immunization strategies against viral pathogens is unclear. We addressed this question in multiple wild-type and genetically altered but clinically relevant mouse strains using several vaccine platforms against influenza viruses, poxviruses and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), a riboflavin-based MR1 ligand of bacterial origin, can synergize with viral vaccines to expand MAIT cells in multiple tissues, reprogram them towards a pro-inflammatory MAIT1 phenotype, license them to bolster virus-specific CD8+ T cell responses, and potentiate heterosubtypic anti-influenza protection. Repeated 5-OP-RU administration did not render MAIT cells anergic, thus allowing for its inclusion in prime-boost immunization protocols. Mechanistically, tissue MAIT cell accumulation was due to their robust proliferation, as opposed to altered migratory behavior, and required viral vaccine replication competency and Toll-like receptor 3 and type I interferon receptor signaling. The observed phenomenon was reproducible in female and male mice, and in both young and old animals. It could also be recapitulated in a human cell culture system in which peripheral blood mononuclear cells were exposed to replicating virions and 5-OP-RU. In conclusion, although viruses and virus-based vaccines are devoid of the riboflavin biosynthesis machinery that supplies MR1 ligands, targeting MR1 enhances the efficacy of vaccine-elicited antiviral immunity. We propose 5-OP-RU as a non-classic but potent and versatile vaccine adjuvant against respiratory viruses.
MeSH term(s)	Female ; Male ; Humans ; Mice ; Animals ; Mucosal-Associated Invariant T Cells ; Vaccine Efficacy ; Leukocytes, Mononuclear ; COVID-19/metabolism ; SARS-CoV-2 ; Riboflavin/metabolism ; Vaccines ; Histocompatibility Antigens Class I ; Minor Histocompatibility Antigens
Chemical Substances	5-(2-oxopropylideneamino)-6-d-ribitylaminouracil ; Riboflavin (TLM2976OFR) ; Vaccines ; Histocompatibility Antigens Class I ; Minor Histocompatibility Antigens ; MR1 protein, human
Language	English
Publishing date	2023-06-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7374
ISSN (online)	1553-7374
ISSN	1553-7374
DOI	10.1371/journal.ppat.1011485
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli.

Duell, Elke R / D'Agostino, Paul M / Shapiro, Nicole / Woyke, Tanja / Fuchs, Thilo M / Gulder, Tobias A M

Microbial cell factories

2019 Volume 18, Issue 1, Page(s) 32

Abstract: Background: Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen ... ...

Abstract	Background: Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a volatile organic compound (VOC) with an unusual polymethylated hydrocarbon bicyclic structure (C Results: Sequencing and mining of the S. plymuthica WS3236 genome revealed the presence of 38 BGCs according to antiSMASH analysis, including a putative sodorifen BGC. Further genome mining for sodorifen and sodorifen-like BGCs throughout bacteria was performed using SodC and SodD as queries and identified a total of 28 sod-like gene clusters. Using direct pathway cloning (DiPaC) we intercepted the 4.6 kb candidate sodorifen BGC from S. plymuthica WS3236 (sodA-D) and transformed it into Escherichia coli BL21. Heterologous expression under the control of the tetracycline inducible Ptet Conclusions: Genome mining of all available genomes within the NCBI and JGI IMG databases led to the identification of a wealth of sod-like pathways which may be responsible for producing a range of structurally unknown sodorifen analogs. Introduction of the S. plymuthica WS3236 sodorifen BGC into the fast-growing heterologous expression host E. coli with a very low VOC background led to a significant increase in both sodorifen product yield and purity compared to the native producer. By providing a reliable, high-level production system, this study sets the stage for future investigations of the biological role and function of sodorifen and for functionally unlocking the bioinformatically identified putative sod-like pathways.
MeSH term(s)	Bacterial Proteins/metabolism ; Biosynthetic Pathways ; Bridged Bicyclo Compounds/metabolism ; Cloning, Molecular ; Computational Biology ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Genome, Bacterial ; Multigene Family ; Octanes/metabolism ; Pyrophosphatases/metabolism ; Serratia/genetics
Chemical Substances	1,2,4,5,6,7,8-heptamethyl-3-methylenebicyclo(3.2.1)oct-6-ene ; Bacterial Proteins ; Bridged Bicyclo Compounds ; Octanes ; Pyrophosphatases (EC 3.6.1.-) ; farnesyl pyrophosphatase (EC 3.6.1.-)
Language	English
Publishing date	2019-02-07
Publishing country	England
Document type	Journal Article
ISSN	1475-2859
ISSN (online)	1475-2859
DOI	10.1186/s12934-019-1080-6
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Article: Antarctic desert soil bacteria exhibit high novel natural product potential, evaluated through long‐read genome sequencing and comparative genomics

Benaud, Nicole / Edwards, Richard J. / Amos, Timothy G. / D'Agostino, Paul M. / Gutiérrez‐Chávez, Carolina / Montgomery, Kate / Nicetic, Iskra / Ferrari, Belinda C.

Environmental microbiology. 2021 July, v. 23, no. 7

2021

Abstract	Actinobacteria and Proteobacteria are important producers of bioactive natural products (NP), and these phyla dominate in the arid soils of Antarctica, where metabolic adaptations influence survival under harsh conditions. Biosynthetic gene clusters (BGCs) which encode NPs, are typically long and repetitious high G + C regions difficult to sequence with short‐read technologies. We sequenced 17 Antarctic soil bacteria from multi‐genome libraries, employing the long‐read PacBio platform, to optimize capture of BGCs and to facilitate a comprehensive analysis of their NP capacity. We report 13 complete bacterial genomes of high quality and contiguity, representing 10 different cold‐adapted genera including novel species. Antarctic BGCs exhibited low similarity to known compound BGCs (av. 31%), with an abundance of terpene, non‐ribosomal peptide and polyketide‐encoding clusters. Comparative genome analysis was used to map BGC variation between closely related strains from geographically distant environments. Results showed the greatest biosynthetic differences to be in a psychrotolerant Streptomyces strain, as well as a rare Actinobacteria genus, Kribbella, while two other Streptomyces spp. were surprisingly similar to known genomes. Streptomyces and Kribbella BGCs were predicted to encode antitumour, antifungal, antibacterial and biosurfactant‐like compounds, and the synthesis of NPs with antibacterial, antifungal and surfactant properties was confirmed through bioactivity assays.
Keywords	Proteobacteria ; Streptomyces ; bioactive properties ; biosynthesis ; desert soils ; genomics ; nonribosomal peptides ; sequence analysis ; surfactants ; Antarctic region ; Antarctica
Language	English
Dates of publication	2021-07
Size	p. 3646-3664.
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.15300
Database	NAL-Catalogue (AGRICOLA)

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