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  1. Book ; Online ; E-Book: Human pluripotent stem cell derived organoid models

    Spence, Jason R.

    (Methods in cell biology ; 159)

    2020  

    Author's details edited by Jason R. Spence
    Series title Methods in cell biology ; 159
    Collection
    Keywords Electronic books
    Language English
    Size 1 Online-Ressource (xiv, 321 Seiten), Illustrationen, Diagramme
    Edition First edition
    Publisher Elsevier Academic Press
    Publishing place Cambridge, Ma
    Publishing country United States
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT020538145
    ISBN 978-0-12-821532-6 ; 9780128215319 ; 0-12-821532-1 ; 0128215313
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article: Clocking the Pace of Organoid Research.

    Spence, Jason R

    Cellular and molecular gastroenterology and hepatology

    2017  Volume 4, Issue 1, Page(s) 203–204

    Language English
    Publishing date 2017-05-13
    Publishing country United States
    Document type Editorial
    ZDB-ID 2819778-1
    ISSN 2352-345X
    ISSN 2352-345X
    DOI 10.1016/j.jcmgh.2017.04.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Taming the Wild West of Organoids, Enteroids, and Mini-Guts.

    Spence, Jason R

    Cellular and molecular gastroenterology and hepatology

    2017  Volume 5, Issue 2, Page(s) 159–160

    Language English
    Publishing date 2017-11-28
    Publishing country United States
    Document type Editorial
    ZDB-ID 2819778-1
    ISSN 2352-345X
    ISSN 2352-345X
    DOI 10.1016/j.jcmgh.2017.11.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Correction to: hPSC-derived organoids: models of human development and disease.

    Frum, Tristan / Spence, Jason R

    Journal of molecular medicine (Berlin, Germany)

    2020  Volume 99, Issue 4, Page(s) 475

    Language English
    Publishing date 2020-11-16
    Publishing country Germany
    Document type Published Erratum
    ZDB-ID 1223802-8
    ISSN 1432-1440 ; 0946-2716
    ISSN (online) 1432-1440
    ISSN 0946-2716
    DOI 10.1007/s00109-020-02007-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Approaches to benchmark and characterize in vitro human model systems.

    Childs, Charlie J / Eiken, Madeline K / Spence, Jason R

    Development (Cambridge, England)

    2022  Volume 149, Issue 20

    Abstract: In vitro human models, such as gastruloids and organoids, are complex three-dimensional (3D) structures often consist of cells from multiple germ layers that possess some attributes of a developing embryo or organ. To use these models to interrogate ... ...

    Abstract In vitro human models, such as gastruloids and organoids, are complex three-dimensional (3D) structures often consist of cells from multiple germ layers that possess some attributes of a developing embryo or organ. To use these models to interrogate human development and organogenesis, these 3D models must accurately recapitulate aspects of their in vivo counterparts. Recent advances in single-cell technologies, including sequencing and spatial approaches, have enabled efforts to better understand and directly compare organoids with native tissues. For example, single-cell genomic efforts have created cell and organ atlases that enable benchmarking of in vitro models and can also be leveraged to gain novel biological insights that can be used to further improve in vitro models. This Spotlight discusses the state of current in vitro model systems, the efforts to create large publicly available atlases of the developing human and how these data are being used to improve organoids. Limitations and perspectives on future efforts are also discussed.
    MeSH term(s) Benchmarking ; Humans ; Organogenesis ; Organoids
    Language English
    Publishing date 2022-10-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.200641
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: hPSC-derived organoids: models of human development and disease.

    Frum, Tristan / Spence, Jason R

    Journal of molecular medicine (Berlin, Germany)

    2020  Volume 99, Issue 4, Page(s) 463–473

    Abstract: Organoids derived from human pluripotent stem cells (hPSCs) have emerged as important models for investigating human-specific aspects of development and disease. Here we discuss hPSC-derived organoids through the lens of development-highlighting how ... ...

    Abstract Organoids derived from human pluripotent stem cells (hPSCs) have emerged as important models for investigating human-specific aspects of development and disease. Here we discuss hPSC-derived organoids through the lens of development-highlighting how stages of human development align with the development of hPSC-derived organoids in the tissue culture dish. Using hPSC-derived lung and intestinal organoids as examples, we discuss the value and application of such systems for understanding human biology, as well as strategies for enhancing organoid complexity and maturity.
    MeSH term(s) Cell Differentiation ; Cell Lineage ; Forecasting ; Germ Layers/cytology ; Humans ; Induced Pluripotent Stem Cells/cytology ; Intestines/cytology ; Lung/cytology ; Organ Specificity ; Organoids/cytology
    Language English
    Publishing date 2020-08-28
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1223802-8
    ISSN 1432-1440 ; 0946-2716
    ISSN (online) 1432-1440
    ISSN 0946-2716
    DOI 10.1007/s00109-020-01969-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Near-Infrared In Vivo Imaging of Claudin-1 Expression by Orthotopically Implanted Patient-Derived Colonic Adenoma Organoids.

    Jaiswal, Sangeeta / Wang, Fa / Wu, Xiaoli / Chang, Tse-Shao / Shirazi, Ahmad / Lee, Miki / Dame, Michael K / Spence, Jason R / Wang, Thomas D

    Diagnostics (Basel, Switzerland)

    2024  Volume 14, Issue 3

    Abstract: Background: Claudin-1 becomes overexpressed during the transformation of normal colonic mucosa to colorectal cancer (CRC).: Methods: Patient-derived organoids expressed clinically relevant target levels and genetic heterogeneity, and were established ...

    Abstract Background: Claudin-1 becomes overexpressed during the transformation of normal colonic mucosa to colorectal cancer (CRC).
    Methods: Patient-derived organoids expressed clinically relevant target levels and genetic heterogeneity, and were established from human adenoma and normal colons. Colonoids were implanted orthotopically in the colon of immunocompromised mice. This pre-clinical model of CRC provides an intact microenvironment and representative vasculature. Colonoid growth was monitored using white light endoscopy. A peptide specific for claudin-1 was fluorescently labeled for intravenous administration. NIR fluorescence images were collected using endoscopy and endomicroscopy.
    Results: NIR fluorescence images collected using wide-field endoscopy showed a significantly greater target-to-background (T/B) ratio for adenoma versus normal (1.89 ± 0.35 and 1.26 ± 0.06) colonoids at 1 h post-injection. These results were confirmed by optical sections collected using endomicroscopy. Optical sections were collected in vivo with sub-cellular resolution in vertical and horizontal planes. Greater claudin-1 expression by individual epithelial cells in adenomatous versus normal crypts was visualized. A human-specific cytokeratin stain ex vivo verified the presence of human tissues implanted adjacent to normal mouse colonic mucosa.
    Conclusions: Increased claudin-1 expression was observed from adenoma versus normal colonoids in vivo using imaging with wide field endoscopy and endomicrosopy.
    Language English
    Publishing date 2024-01-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics14030273
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Stably-Inverted Apical-Out Human Upper Airway Organoids for SARS-CoV-2 Infection and Therapeutic Testing.

    Lee, Ji-Hoon / LeCher, Julia C / Parigoris, Eric / Shinagawa, Noriyuki / Sentosa, Jason / Manfredi, Candela / Goh, Shu Ling / De, Ramyani / Tao, Sijia / Zandi, Keivan / Amblard, Franck / Sorscher, Eric J / Spence, Jason R / Tirouvanziam, Rabindra / Schinazi, Raymond F / Takayama, Shuichi

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Apical-out organoids produced through eversion triggered by extra-organoid extracellular matrix (ECM) removal or degradation are generally small, structurally variable, and limited for viral infection and therapeutics testing. This work describes ECM- ... ...

    Abstract Apical-out organoids produced through eversion triggered by extra-organoid extracellular matrix (ECM) removal or degradation are generally small, structurally variable, and limited for viral infection and therapeutics testing. This work describes ECM-encapsulating, stably-inverted apical-out human upper airway organoids (AORBs) that are large (~500 μm diameter), consistently spherical, recapitulate
    Language English
    Publishing date 2024-01-04
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.01.02.573939
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Studying SARS-CoV-2 infectivity and therapeutic responses with complex organoids.

    Chen, Kevin G / Park, Kyeyoon / Spence, Jason R

    Nature cell biology

    2021  Volume 23, Issue 8, Page(s) 822–833

    Abstract: Clinical management of patients with severe complications of COVID-19 has been hindered by a lack of effective drugs and a failure to capture the extensive heterogeneity of the disease with conventional methods. Here we review the emerging roles of ... ...

    Abstract Clinical management of patients with severe complications of COVID-19 has been hindered by a lack of effective drugs and a failure to capture the extensive heterogeneity of the disease with conventional methods. Here we review the emerging roles of complex organoids in the study of SARS-CoV-2 infection, modelling of COVID-19 disease pathology and in drug, antibody and vaccine development. We discuss opportunities for COVID-19 research and remaining challenges in the application of organoids.
    MeSH term(s) Antibodies, Viral/immunology ; Antibodies, Viral/metabolism ; COVID-19/immunology ; COVID-19/metabolism ; COVID-19/prevention & control ; COVID-19 Vaccines/immunology ; COVID-19 Vaccines/therapeutic use ; Humans ; Organoids/metabolism ; SARS-CoV-2/immunology ; SARS-CoV-2/pathogenicity
    Chemical Substances Antibodies, Viral ; COVID-19 Vaccines
    Language English
    Publishing date 2021-08-02
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural ; Review
    ZDB-ID 1474722-4
    ISSN 1476-4679 ; 1465-7392
    ISSN (online) 1476-4679
    ISSN 1465-7392
    DOI 10.1038/s41556-021-00721-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Rapid establishment of human colonic organoid knockout lines.

    Gu, Wei / Colarusso, Jonathan L / Dame, Michael K / Spence, Jason R / Zhou, Qiao

    STAR protocols

    2022  Volume 3, Issue 2, Page(s) 101308

    Abstract: Human colonic organoids derived from biopsy or autopsy tissues are a vital tool to study mucosal homeostasis, model colonic diseases, and develop therapeutics. Rapid and reliable generation of knockout organoid lines from multiple donors enables analysis ...

    Abstract Human colonic organoids derived from biopsy or autopsy tissues are a vital tool to study mucosal homeostasis, model colonic diseases, and develop therapeutics. Rapid and reliable generation of knockout organoid lines from multiple donors enables analysis of specific gene functions. Here, we report protocols to produce colonic organoid knockout lines within 1 to 2 weeks using lentiviral delivery of CRISPR-Cas9, achieving knockout efficiency of 90% or greater. These lines are suitable for multi-lineage differentiation and downstream analysis. For complete details on the use and execution of this protocol, please refer to Gu et al. (2022).
    MeSH term(s) Biopsy ; Colon ; Humans ; Organoids
    Language English
    Publishing date 2022-04-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2022.101308
    Database MEDical Literature Analysis and Retrieval System OnLINE

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