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Article ; Online: The rs2910164:G>C SNP in the MIR146A gene is not associated with breast cancer risk in BRCA1 and BRCA2 mutation carriers.

Garcia, Amandine I / Cox, David G / Barjhoux, Laure / Verny-Pierre, Carole / Barnes, Daniel / Antoniou, Antonis C / Stoppa-Lyonnet, Dominique / Sinilnikova, Olga M / Mazoyer, Sylvie

Human mutation

2011 Volume 32, Issue 9, Page(s) 1004–1007

Abstract: The rs2910164:G>C SNP is located in the gene for miR-146a, a microRNA that binds the 3' ... mutation. We show here a lack of association of the rs2910164:G>C SNP with breast cancer risk in a series ...

Abstract	The rs2910164:G>C SNP is located in the gene for miR-146a, a microRNA that binds the 3' UTR of the BRCA1 transcript. Preliminary data based on the analysis of a small number of cases suggested that this single nucleotide polymorphism (SNP) might be associated with the age of onset of familial breast and ovarian cancer. This effect was not confirmed on a large series of familial breast cancer cases negative for a BRCA1 or BRCA2 mutation. We show here a lack of association of the rs2910164:G>C SNP with breast cancer risk in a series of 1,166 BRCA1 and 560 BRCA2 mutation carriers. In conclusion, the polymorphism in the miR-146a gene is unlikely to be of substantial significance regarding breast cancer risk.
MeSH term(s)	Adult ; BRCA1 Protein/genetics ; BRCA2 Protein/genetics ; Breast Neoplasms/genetics ; Case-Control Studies ; Female ; Genetic Association Studies ; Genetic Predisposition to Disease ; Heterozygote ; Humans ; MicroRNAs/genetics ; Mutation/genetics ; Polymorphism, Single Nucleotide/genetics ; Risk Factors
Chemical Substances	BRCA1 Protein ; BRCA1 protein, human ; BRCA2 Protein ; BRCA2 protein, human ; MIRN146 microRNA, human ; MicroRNAs
Language	English
Publishing date	2011-07-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1126646-6
ISSN	1098-1004 ; 1059-7794
ISSN (online)	1098-1004
ISSN	1059-7794
DOI	10.1002/humu.21539
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Zs.A 3586: Show issues

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Article: Insight into externally bound 5,10,15,20-tetrakis(2-N-methylpyridyl) porphyrinatopalladium(II), PdP(2), with B-form DNA duplexes poly(G-c)2, poly(A-t)2, and CT DNA by using combined MCD, CD, and optical data.

Barnes, N R / Schreiner, A F / Finnegan, M G / Johnson, M K

Biospectroscopy

1998 Volume 4, Issue 5, Page(s) 341–352

Abstract: ... magnitude of 1a1u-3a2u, or delta HOMO. For the third system, PdP(2)/poly (G-C)2, the B0 CD spectrum has two ...

Abstract	The Soret (B0) region of free and externally DNA-bound 5,10,15,20-tetrakis(2-N-methylpyridyl) porphyrinatopalladium(II), PdP(2), was investigated by electronic magnetic circular dichroism (MCD), natural circular dichroism (CD), and optical (UV-visible) absorption spectroscopies. We conclude that four-coordinate, "thick" PdP(2) binds to the exterior of each of poly(A-T)2 and calf thymus DNA (CT DNA) by two distinctly different AT-specific minor and major groove modes, with site 5'TA3' being favored for both modes. The minor groove mode involves an edge-on orientation of PdP(2), for which porphyrin electric dipole transition moments (edtms) mu x (most perturbed direction of the bound porphyrin) and mu y (least perturbed direction) have approximate orientation angles of alpha/beta/beta' = approximately 90 degrees/0 degree/0 degree and approximately 45 degrees/0 degree/90 degrees, respectively. Major groove binding is by a face-on mode, which results in the porphyrin plane being approximately parallel to the helix axis, such that mu x (most perturbed direction) and mu y (least perturbed direction) have approximate orientation angles of alpha/beta/beta' = approximately 45 degrees/180 degrees/90 degrees and approximately 45 degrees/180 degrees/270 degrees, respectively. The Soret MCD and optical band alterations upon binding (i.e., sign retention of the tetragonal, genuine MCD (+) A-term on becoming the (+) pseudo-A-term of similar amplitude and small DNA-induced optical red (delta lambda) and hypochromic (H) shifts) are all consistent with exterior binding perturbations of the porphyrin's p pi MOs (1a1u 3a2u 4eg) by the A and T bases of each polymer being weaker than caused by intercalation. Furthermore, that the (+) A-term of PdP(2) retains the (+) sign upon binding informs that the 4eg splitting, or delta LUMO, is less than the energy separation magnitude of 1a1u-3a2u, or delta HOMO. For the third system, PdP(2)/poly (G-C)2, the B0 CD spectrum has two extremely weak (+) and (-) CD bands at higher and lower energy, respectively, indicating that weak outside binding (wob) interactions are taking place between the cationic porphyrin and the electron-rich phosphate backbone of this rigid polymer. The composite of our CD, MCD, and optical data are suggestive of a face-on mode at the GC major groove. Band parameter extraction is performed on the Soret CD and MCD bands of each of the three bound systems, and it is determined that (1) very little spatial rotation of molecular charge is induced during CD excitation and (2) the excited state angular momentum, 'Lj', changes very little upon binding of PdP(2) to each duplex. These findings are also consistent with each PdP(2)/B-DNA interaction not being very strong.
MeSH term(s)	Base Pairing ; Binding Sites ; Circular Dichroism ; DNA/metabolism ; Magnetics ; Metalloporphyrins/chemistry ; Metalloporphyrins/metabolism ; Nucleic Acid Heteroduplexes/chemistry ; Nucleic Acid Heteroduplexes/metabolism ; Palladium ; Pyridines/chemistry ; Pyridines/metabolism ; Spectrophotometry/methods
Chemical Substances	5,10,15,20-tetrakis(2-N-methylpyridyl)porphyrinatopalladium(II) ; Metalloporphyrins ; Nucleic Acid Heteroduplexes ; Pyridines ; Palladium (5TWQ1V240M) ; DNA (9007-49-2)
Language	English
Publishing date	1998
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	1302703-7
ISSN	1075-4261
ISSN	1075-4261
DOI	10.1002/(SICI)1520-6343(1998)4:5%3C341::AID-BSPY5%3E3.0.CO;2-E
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Zs.A 4678: Show issues

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Article: Spontaneous mutation frequencies in Salmonella: enhancement of G/C to A/T transitions and depression of deletion and frameshift mutation frequencies afforded by anoxic incubation.

Hartman, Z / Hartman, P E / Barnes, W M / Tuley, E

Environmental mutagenesis

1984 Volume 6, Issue 5, Page(s) 633–650

Abstract: ... in the hisG428 ochre mutant and also decreases the frequency of minus frameshift mutations in G/C-rich sequences ... in the his-D3052 and hisC3076 mutants. In contrast, the frequency of G/C-----A/T transition mutations increases ...

Abstract	Incubation of Salmonella typhimurium under anoxic conditions (0.1% oxygen or less) results in a substantial decrease in small (3-and 6-basepair) deletions in an A/T-rich region of the hisG gene in the hisG428 ochre mutant and also decreases the frequency of minus frameshift mutations in G/C-rich sequences in the his-D3052 and hisC3076 mutants. In contrast, the frequency of G/C-----A/T transition mutations increases substantially during anoxic growth of hisG46. Growth of revertants of strains carrying accessory deletions in the uvrB region of the Salmonella chromosome is drastically impaired on glucose minimal medium when oxygen partial pressures are below 0.1% oxygen.
MeSH term(s)	Aerobiosis ; Anaerobiosis ; Chromosome Deletion ; Genotype ; Mutation ; Oxygen/pharmacology ; Salmonella typhimurium/drug effects ; Salmonella typhimurium/genetics ; Salmonella typhimurium/growth & development ; Species Specificity
Chemical Substances	Oxygen (S88TT14065)
Language	English
Publishing date	1984
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	392053-7
ISSN	0192-2521
ISSN	0192-2521
DOI	10.1002/em.2860060503
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Article ; Online: Strategies for the Use of Dupilumab in Eosinophilic Esophagitis.

Sauer, Bryan G / Barnes, Barrett H / McGowan, Emily C

The American journal of gastroenterology

2023 Volume 118, Issue 5, Page(s) 780–783

MeSH term(s)	Humans ; Eosinophilic Esophagitis/drug therapy ; Antibodies, Monoclonal, Humanized/therapeutic use ; Enteritis
Chemical Substances	dupilumab (420K487FSG) ; Antibodies, Monoclonal, Humanized
Language	English
Publishing date	2023-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	390122-1
ISSN	1572-0241 ; 0002-9270
ISSN (online)	1572-0241
ISSN	0002-9270
DOI	10.14309/ajg.0000000000002206
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Article: Cholera toxin and dibutyryl cyclic adenosine 3',5'-monophosphate sensitize gonadotropin-releasing hormone-stimulated inositol phosphate production to inhibition in protein kinase-C (PKC)-depleted cells: evidence for cross-talk between a cholera toxin-sensitive G-protein and PKC.

Barnes, S J / Conn, P M

Endocrinology

1993 Volume 133, Issue 6, Page(s) 2756–2760

Abstract: The present study assesses the relationship between G-proteins and protein kinase-C (PKC ... phospholipase-C at distinct sites. CTX activates a G-protein that increases cAMP. cAMP can then activate PKA ... between a CTX-sensitive G-protein and PKC. ...

Abstract	The present study assesses the relationship between G-proteins and protein kinase-C (PKC) in the gonadotrope. Cells were depleted of PKC with 1 microM phorbol 12-myristate 13-acetate for 12 h, followed by medium 199-BSA for 6 h before treatment with vehicle, pertussis toxin (PTX), cholera toxin (CTX), or (Bu)2cAMP (dBcAMP) for 18 h. PTX (10 ng/ml) significantly decreased GnRH-stimulated inositol phosphate (IP) production over a range of 10(-8)-10(-6) M GnRH. The degree of this inhibition was the same in control cells and PKC-depleted cells. Pretreatment with CTX (0.5 microgram/ml) significantly decreased GnRH-stimulated IP production over a range of 10(-9)-10(-6) M GnRH in PKC-depleted cells. This effect was mimicked by pretreatment with 3 mM dBcAMP. Although CTX and dBcAMP both decreased GnRH-stimulated IP production in control cells, this effect was enhanced in PKC-depleted cells. CTX (0.1 microgram/ml) and dBcAMP (3 mM) both enhanced GnRH-stimulated LH release, whereas PTX (100 ng/ml) had no effect. This was observed in control as well as PKC-depleted cells. Both PKA and PKC are capable of regulating IP turnover by phosphorylating phospholipase-C at distinct sites. CTX activates a G-protein that increases cAMP. cAMP can then activate PKA. In PKC-depleted cells, CTX inhibits GnRH-stimulated IP production. This effect is mimicked by dBcAMP, which suggests a role for PKA in the gonadotrope. The results of this study provide evidence for cross-talk between a CTX-sensitive G-protein and PKC.
MeSH term(s)	Animals ; Bucladesine/pharmacology ; Cells, Cultured ; Cholera Toxin/pharmacology ; Gonadotropin-Releasing Hormone/pharmacology ; Inositol Phosphates/antagonists & inhibitors ; Inositol Phosphates/biosynthesis ; Luteinizing Hormone/metabolism ; Pertussis Toxin ; Pituitary Gland/cytology ; Pituitary Gland/metabolism ; Protein Kinase C/deficiency ; Rats ; Virulence Factors, Bordetella/pharmacology
Chemical Substances	Inositol Phosphates ; Virulence Factors, Bordetella ; Gonadotropin-Releasing Hormone (33515-09-2) ; Bucladesine (63X7MBT2LQ) ; Luteinizing Hormone (9002-67-9) ; Cholera Toxin (9012-63-9) ; Pertussis Toxin (EC 2.4.2.31) ; Protein Kinase C (EC 2.7.11.13)
Language	English
Publishing date	1993-12
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	427856-2
ISSN	1945-7170 ; 0013-7227
ISSN (online)	1945-7170
ISSN	0013-7227
DOI	10.1210/endo.133.6.8243300
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Book ; Online ; E-Book: Air pollution studies

Passerini, G. / Borrego, Carlos / Longhurst, J. W. S. / Lopes, M. / Barnes, J.

2020

Author's details	editors G. Passerini, C. Borrego, J. Longhurst, M. Lopes, J. Barnes
Keywords	Electronic books
Language	English
Size	1 Online-Ressource (ix, 139 Seiten), Illustrationen
Publisher	WIT Press
Publishing place	Southampton
Publishing country	Great Britain
Document type	Book ; Online ; E-Book
Note	Description based on publisher supplied metadata and other sources
Remark	Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
HBZ-ID	HT020554757
ISBN	978-1-78466-374-2 ; 9781784663735 ; 1-78466-374-3 ; 1784663735
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Simulation training to retool practicing orthopedic surgeons is rare.

Cate, Graham / Barnes, C Lowry / Dickinson, Karen J

Global surgical education : journal of the Association for Surgical Education

2023 Volume 2, Issue 1, Page(s) 57

Abstract: Purpose: Modern surgical practice is continuously changing as technology develops. New techniques are often implemented after a surgeon has made the transition to independent clinical practice. There is therefore a need to 'retool' technical skills. ... ...

Abstract	Purpose: Modern surgical practice is continuously changing as technology develops. New techniques are often implemented after a surgeon has made the transition to independent clinical practice. There is therefore a need to 'retool' technical skills. Additionally, practicing surgeons must maintain and develop skills such as leadership, communication, critical thinking, teaching, and mentoring. Our aim was to perform a scoping review to assess the current status of simulation education for practicing Orthopedic Surgeons (OS). Methods: A 10 year search of PubMed, ERIC, and Web of Science was performed with a medical librarian. Controlled vocabulary Medical Subject Headings terms and natural language were developed with subject matter experts describing simulation, training and OS. Two trained reviewers evaluated all abstracts for inclusion. Exclusion criteria were articles that did not assess simulation education involving practicing OS. Data were extracted from the included full text articles by two reviewers: details of study design, type of participants, type of simulation and role of OS in the educational event. Results: Initial search identified 1824 articles of which 443 were duplicates, and 1381 articles were further screened. Of these, 1155 were excluded, 226 full text articles were assessed for eligibility and 80 included in analysis. Most were published in the last 6 years and from the United States. The majority (99%) described technical skill simulations (arthroscopy 56%, screw placement 23%, ligament reconstruction 19%). OS were rarely the only learners with 91% studies also having residents participate. OS were the targeted learner in 6% studies. OS provided content validity for 15 (19%) and construct validity in 59 (74%) studies. Conclusions: Simulation training to educate practicing OS is rare. OS are often used to validate work rather than being the center of an educational endeavor. A refocusing is needed to provide adequate training for practicing surgeons to retool skills as new techniques become available.
Language	English
Publishing date	2023-05-23
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2731-4588
ISSN (online)	2731-4588
DOI	10.1007/s44186-023-00136-2
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Article: Simultaneous immunization with B.C.G., diphtheria-tetanus, and oral poliomyelitis vaccines in children aged 13-14.

Galbraith, N S / Crosby, G / Barnes, J M / Fernandes, R

British medical journal

1971 Volume 2, Issue 5755, Page(s) 193–197

Abstract: The simultaneous administration of B.C.G. vaccine, diphtheria-tetanus toxoid aluminium hydroxide ... the attainment of high immunization rates with the vaccines given separately. No adverse reactions to B.C.G. or ... administration of tetanus toxoid aluminium hydroxide adsorbed, oral poliomyelitis, and B.C.G. vaccines only is ...

Abstract	The simultaneous administration of B.C.G. vaccine, diphtheria-tetanus toxoid aluminium hydroxide adsorbed vaccine, and oral poliovaccine was studied in 628 children aged 13-14 years between 1966 and 1969 in Newham, London. The efficacy of these vaccines was unaffected by administering them at the same time; routine simultaneous administration is considered justified when organizational difficulties prevent the attainment of high immunization rates with the vaccines given separately. No adverse reactions to B.C.G. or oral poliomyelitis vaccines took place, but 8% of children had moderately severe local reactions after diphtheria-tetanus aluminium hydroxide adsorbed vaccine, which were attributed to diphtheria toxoid.Serological studies showed the need for immunization against diphtheria, tetanus, and poliomyelitis at 13-14 years of age. Because of the adverse reactions to diphtheria toxoid, however, simultaneous administration of tetanus toxoid aluminium hydroxide adsorbed, oral poliomyelitis, and B.C.G. vaccines only is recommended at present.An "adult type" diphtheria-tetanus toxoid might overcome the problem of reactions, though in two to three years' time most children aged 13-14 years will have received diphtheria-tetanus-pertussis vaccine in infancy and reinforcement might then be accomplished by a small intradermal dose of the currently available fluid diphtheria-tetanus vaccine.Continued serological studies of diphtheria and tetanus antitoxins and polio antibody are necessary to determine the future need for reinforcement of immunity; such studies should become an essential part of the surveillance of the community immunization programme.
MeSH term(s)	Adolescent ; Adult ; Antibodies/analysis ; Antitoxins/analysis ; BCG Vaccine ; Child ; Diphtheria Toxoid/administration & dosage ; Diphtheria Toxoid/adverse effects ; Hemagglutination Inhibition Tests ; Humans ; Immunization ; London ; Neutralization Tests ; Poliovirus Vaccine, Oral/administration & dosage ; Preventive Health Services ; Rubella/immunology ; Tetanus Toxoid/administration & dosage
Chemical Substances	Antibodies ; Antitoxins ; BCG Vaccine ; Diphtheria Toxoid ; Poliovirus Vaccine, Oral ; Tetanus Toxoid
Language	English
Publishing date	1971-04-24
Publishing country	England
Document type	Journal Article
ZDB-ID	80088-0
ISSN	0007-1447 ; 0267-0623 ; 0959-8138 ; 0959-8146
ISSN	0007-1447 ; 0267-0623 ; 0959-8138 ; 0959-8146
DOI	10.1136/bmj.2.5755.193
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Article: The Treatment of Gout-1967.

Barnes, C G / Mason, R M

Journal of the Royal College of Physicians of London

2019 Volume 1, Issue 4, Page(s) 427–441

Language	English
Publishing date	2019-01-22
Publishing country	England
Document type	Journal Article
ZDB-ID	3005-3
ISSN	0035-8819
ISSN	0035-8819
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Article: Successful Transition to Same Calendar Day Discharge in Total Joint Arthroplasty at an Academic Center.

Mathews, Candler G / Stambough, Jeffrey B / Stronach, Benjamin / Siegel, Eric R / Barnes, C Lowry / Mears, Simon C

Arthroplasty today

2024 Volume 27, Page(s) 101354

Abstract: Background: There has been a shift toward same-day discharge (SDD) in total joint arthroplasty (TJA) in recent years. Our clinical standard had been next-day discharge, but the COVID pandemic led to a hospital bed shortage, causing us to shift to SDD ... ...

Abstract	Background: There has been a shift toward same-day discharge (SDD) in total joint arthroplasty (TJA) in recent years. Our clinical standard had been next-day discharge, but the COVID pandemic led to a hospital bed shortage, causing us to shift to SDD directly from the Post-Anesthesia Care Unit (PACU). The aim of our project was to investigate if the SDD protocol was successful and if it changed complications or 90-day readmission rates. Our secondary aim was to investigate if the protocol created disparities in patient selection. Methods: A retrospective review compared the first 100 patients intended to discharge from PACU to the 100 patients prior to the SDD protocol undergoing elective primary TJA procedures at our academic institution from September 1, 2020, to March 23, 2021. The SDD protocol started on November 19, 2020. Results: During this SDD period, 98% (98/100) of patients were successfully discharged from the PACU. The 90-day readmission rate changed from 0% to 2% ( Conclusions: The transition to same SDD in TJA at our academic institution was successfully implemented without markedly increasing complications, readmissions, or changing patient selection. The COVID-19 pandemic likely influenced the recovery of patients before and after the protocol. Future studies are needed to validate this data during the post-COVID era.
Language	English
Publishing date	2024-03-16
Publishing country	United States
Document type	Journal Article
ISSN	2352-3441
ISSN	2352-3441
DOI	10.1016/j.artd.2024.101354
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