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  1. Article ; Online: Nursing students' perceptions of clinical relevance and engagement with bioscience education: A cross-sectional study of undergraduate and postgraduate nursing students.

    Barton, Matthew J / Bentley, Steven / Craft, Judy / Dupen, Oliver / Gordon, Christopher / Cayanan, Elizabeth A / Kunst, Elicia / Connors, Amanda / Todorovic, Michael / Johnston, Amy Nb

    Nurse education today

    2021  Volume 99, Page(s) 104767

    Abstract: ... undertaking undergraduate nursing studies (1st, 2nd & 3rd years), and postgraduate nursing studies.: Results ...

    Abstract Background: Bioscience in nursing education covers a broad range of disciplinary areas (anatomy, physiology, pathophysiology and pharmacology) and underpins clinical assessment and critical thinking in nursing practice. This is imperative for patient safety and favourable patient outcomes. In nursing programs for registration, most bioscience content is taught during the early phases of the program and little incorporated into postgraduate nursing programs.
    Objectives: The aim of this study was to explore student's perceived relationship between clinical relevance and engagement (attention and time) with bioscience content, by surveying undergraduates and postgraduate nursing students.
    Design: This sequential mixed methods study included two phases.
    Methods: The first phase comprised of focus group interviews from one Australian University. Thematic analysis of these data, coupled with existing literature, informed the second study phase; a quantitative questionnaire.
    Participants: Focus group interviews included N = 30 students from one tertiary site; 10 from each year level. The questionnaire was administered to nursing students undertaking undergraduate nursing studies (1st, 2nd & 3rd years), and postgraduate nursing studies.
    Results: Nursing students (n = 406) across three Australian universities (four programmes) completed the questionnaire. The clinical relevance of bioscience was widely appreciated; 91.6% of undergraduate nursing students and 98.5% of postgraduate nursing students indicated that every nurse must have a good understanding of bioscience. However, there was an inverse relationship between engagement with bioscience and timing in the curriculum, as 50% of undergraduate nursing students indicated that bioscience content took up too much of their time, compared to only 20% of postgraduate nurses (odds ratio 0.27 [0.16-0.46], p < 0.001).
    Conclusion: Nursing students' perceptions of the clinical relevance of bioscience for their career strongly corresponds with their progression through their studies. Unfortunately, as students progress to the later years of their nursing education, their engagement with formal bioscience education decreases. This poses the question 'Are we delivering bioscience content to nursing students at the appropriate time?'
    MeSH term(s) Australia ; Cross-Sectional Studies ; Curriculum ; Education, Nursing, Baccalaureate ; Humans ; Perception ; Students, Nursing ; Surveys and Questionnaires
    Language English
    Publishing date 2021-01-26
    Publishing country Scotland
    Document type Journal Article
    ZDB-ID 1062570-7
    ISSN 1532-2793 ; 0260-6917
    ISSN (online) 1532-2793
    ISSN 0260-6917
    DOI 10.1016/j.nedt.2021.104767
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Structures of HIV-1 Neutralizing Antibody 10E8 Delineate the Mechanistic Basis of Its Multi-Peak Behavior on Size-Exclusion Chromatography.

    Do Kwon, Young / Wang, Xiangchun E / Bender, Michael F / Yang, Rong / Li, Yile / McKee, Krisha / Rawi, Reda / O'Dell, Sijy / Schneck, Nicole A / Shaddeau, Andrew / Zhang, Baoshan / Arnold, Frank J / Connors, Mark / Doria-Rose, Nicole A / Kwong, Peter D / Lei, Q Paula

    Antibodies (Basel, Switzerland)

    2021  Volume 10, Issue 2

    Abstract: ... motif in the heavy chain 3rd complementarity-determining region (CDR H3) of antibody 10E8 to be ...

    Abstract Antibody 10E8 is capable of effectively neutralizing HIV through its recognition of the membrane-proximal external region (MPER), and a suitably optimized version of 10E8 might have utility in HIV therapy and prophylaxis. However, 10E8 displays a three-peak profile on size-exclusion chromatography (SEC), complicating its manufacture. Here we show cis-trans conformational isomerization of the Tyr-Pro-Pro (YPP) motif in the heavy chain 3rd complementarity-determining region (CDR H3) of antibody 10E8 to be the mechanistic basis of its multipeak behavior. We observed 10E8 to undergo slow conformational isomerization and delineate a mechanistic explanation for effective comodifiers that were able to resolve its SEC heterogeneity and to allow an evaluation of the critical quality attribute of aggregation. We determined crystal structures of single and double alanine mutants of a key di-proline motif and of a light chain variant, revealing alternative conformations of the CDR H3. We also replicated both multi-peak and delayed SEC behavior with MPER-antibodies 4E10 and VRC42, by introducing a Tyr-Pro (YP) motif into their CDR H3s. Our results show how a conformationally dynamic CDR H3 can provide the requisite structural plasticity needed for a highly hydrophobic paratope to recognize its membrane-proximal epitope.
    Language English
    Publishing date 2021-06-10
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2073-4468
    ISSN (online) 2073-4468
    DOI 10.3390/antib10020023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: The influence of growth on development outcome in extremely low birthweight infants at 2 years of age.

    Connors, J M / O'Callaghan, M J / Burns, Y R / Gray, P H / Tudehope, D I / Mohay, H / Rogers, Y M

    Journal of paediatrics and child health

    1999  Volume 35, Issue 1, Page(s) 37–41

    Abstract: Objective: To determine if weight < 3rd and < 10th centile at 2 years in extremely low birthweight ... for adverse developmental outcome based on perinatal risk factors. Weight at 2 years was classified as < 3rd ... 3rd-9th or > or = 10th centile for age and gender. Development was assessed using the Griffiths Mental ...

    Abstract Objective: To determine if weight < 3rd and < 10th centile at 2 years in extremely low birthweight (ELBW) infants is associated with problems of development and motor skills, and whether this association is explained by perinatal risk status.
    Methodology: One hundred and ninety-eight of 226 (88%) surviving ELBW infants born between January 1987 and December 1992 were assessed at 2 years corrected age. Children were classified as being at low perinatal risk (n = 128) or high perinatal risk (n = 70) for adverse developmental outcome based on perinatal risk factors. Weight at 2 years was classified as < 3rd, 3rd-9th or > or = 10th centile for age and gender. Development was assessed using the Griffiths Mental Developmental Scales and motor skills using the Neurosensory Motor Developmental Assessment (NSMDA).
    Results: For the total study group weight centile was strongly related to General Quotient (GQ) and motor abilities. For children < 3rd percentile (n = 48) mean (GQ) was 90.4 (SD, 15.9), for children between the 3rd-9th percentile (n = 49) 91.5 (SD, 17.9), and for children > or = 10th percentile (n = 99) mean GQ was 99.8 (SD, 8.6). The association with mean GQ and NSMDA category occurred for the high-risk subgroup and became non-significant in the low-risk subgroup if neurologically abnormal children were excluded. Other perinatal risk factors, exposure to breast milk, level of maternal education, marital status and history of feeding problems or infections over the 2 years did not confound this association.
    Conclusion: Low weight percentile at 2 years was related to adverse developmental outcome in ELBW infants at high perinatal risk or with neurological impairment, though minimal association was present for neurologically normal infants at low perinatal risk.
    MeSH term(s) Birth Weight ; Breast Feeding ; Child, Preschool ; Developmental Disabilities/etiology ; Educational Status ; Female ; Follow-Up Studies ; Humans ; Infant, Newborn ; Infant, Very Low Birth Weight/growth & development ; Male ; Marital Status ; Mothers/education ; Mothers/statistics & numerical data ; Predictive Value of Tests ; Prognosis ; Psychomotor Performance ; Risk Factors ; Weight Gain
    Language English
    Publishing date 1999-02
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 1024476-1
    ISSN 1440-1754 ; 1034-4810
    ISSN (online) 1440-1754
    ISSN 1034-4810
    DOI 10.1046/j.1440-1754.1999.00309.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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