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  1. Article ; Online: Observation on the ice crystal formation process of large yellow croaker (Pseudosciaena crocea) and the effect of multiple cryoprotectants pre-soaking treatments on frozen quality.

    Yang, Zhikang / Ye, Guosen / Yang, Dazhang / Xie, Jing / Huo, Yilin

    Cryobiology

    2023  Volume 113, Page(s) 104580

    Abstract: By observing the formation behavior of ice crystals, the quality of food products under different freezing conditions can be intuitively judged. In this paper, large yellow croaker was taken as the research object, and a novel cryomicroscopic system was ... ...

    Abstract By observing the formation behavior of ice crystals, the quality of food products under different freezing conditions can be intuitively judged. In this paper, large yellow croaker was taken as the research object, and a novel cryomicroscopic system was developed to directly observe the structure of ice crystals during the freezing process. The cryoprotective effects of 4% sucrose +4% sorbitol (SU + SO), 4% xylo-oligosaccharide (XO), 4% xylo-oligosaccharide + 0.3% tetrasodium pyrophosphate (XO + TSPP) and 0.2% antifreeze protein (AFP) at different freezing temperatures were investigated. And the evaluation indicators, such as cell deformation degree, equivalent diameters, roundness, elongation and fractal dimension were introduced to quantify the damage of ice crystals to muscle tissues and fibers. The results indicate that reducing the freezing temperature and adding cryoprotectants can improve the quality of large yellow croaker. AFP has the best cryoprotective effect, with a reduction in cell deformation degree of 54.78% and 67.83% compared to the Control group at -5 °C and -20 °C, respectively. SU + SO and XO have the equivalent antifreeze effect, which is slightly inferior to XO + TSPP. In addition, physical parameters of large yellow croaker samples were measured to verify the influence of ice crystal structure on product quality. Therefore, direct observation of the ice crystal formation process and evaluation of ice crystal structure can accurately reflect the quality of frozen products, which is of great significance for the development of refrigeration and preservation technology.
    MeSH term(s) Animals ; Freezing ; Cryoprotective Agents/pharmacology ; Cryoprotective Agents/chemistry ; Ice ; alpha-Fetoproteins ; Cryopreservation/methods ; Perciformes ; Antifreeze Proteins/pharmacology ; Oligosaccharides/chemistry
    Chemical Substances Cryoprotective Agents ; Ice ; alpha-Fetoproteins ; Antifreeze Proteins ; Oligosaccharides
    Language English
    Publishing date 2023-08-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 80098-3
    ISSN 1090-2392 ; 0011-2240
    ISSN (online) 1090-2392
    ISSN 0011-2240
    DOI 10.1016/j.cryobiol.2023.104580
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: IsoSplitter: identification and characterization of alternative splicing sites without a reference genome.

    Wang, Yupeng / Hu, Zhikang / Ye, Ning / Yin, Hengfu

    RNA (New York, N.Y.)

    2021  

    Abstract: Long-read transcriptome sequencing is designed to sequence full-length RNA molecules and advantageous for identifying alternative splice isoforms; however, in the absence of a reference genome, it is difficult to accurately locate splice sites, because ... ...

    Abstract Long-read transcriptome sequencing is designed to sequence full-length RNA molecules and advantageous for identifying alternative splice isoforms; however, in the absence of a reference genome, it is difficult to accurately locate splice sites, because of the diversity of patterns of alternative splicing (AS). Based on long-read transcriptome data we developed a versatile tool, IsoSplitter, to reverse-trace and validate AS gene "split-sites" with the following features: (1) IsoSplitter initially invokes a modified SIM4 program to find transcript split-sites; (2) each split-site is then quantified, to reveal transcript diversity, and putative isoforms are grouped into gene clusters; (3) an optional step for aligning short-reads is provided, to validate split-sites by identifying unique junction reads, and revealing and quantifying tissue-specific alternative splice isoforms. We tested IsoSplitter AS prediction using datasets from multiple model and non-model plant species, and showed that IsoSplitter pipeline is efficient to handle different transcriptomes with high accuracy. Furthermore, we evaluated the IsoSplitter pipeline compared with that of the splice junction identification tools, Program to Assemble Spliced Alignments (PASA-software needs a reference genome for AS identification) and AStrap, using data from the model plant Arabidopsis thaliana. We found that, IsoSplitter determined more than twice as many AS events than AStrap analysis; and 94.13% of the IsoSplitter predicted AS events were also identified by the PASA analysis. Starting from a simple sequence file, IsoSplitter is an assembly-free tool for identification and characterization of AS. IsoSplitter is developed and implemented in Python 3.5 using the Linux platform and is freely available at https://github.com/Hengfu-Yin/IsoSplitter.
    Language English
    Publishing date 2021-05-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1241540-6
    ISSN 1469-9001 ; 1355-8382
    ISSN (online) 1469-9001
    ISSN 1355-8382
    DOI 10.1261/rna.077834.120
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Amino acid-based metallo-supramolecular nanoassemblies capable of regulating cellular redox homeostasis for tumoricidal chemo-/photo-/catalytic combination therapy.

    Tu, Li / Chen, Shengqiang / Yuan, Zhikang / Xiong, Yeqi / Luo, Bingkun / Chen, Ying / Hou, Zhenqing / Ke, Sunkui / Lin, Naibo / Li, Chao / Ye, Shefang

    Journal of colloid and interface science

    2024  Volume 663, Page(s) 810–824

    Abstract: Nanozymes, as nanomaterials with natural enzyme activities, have been widely applied to deliver various therapeutic agents to synergistically combat the progression of malignant tumors. However, currently common inorganic nanozyme-based drug delivery ... ...

    Abstract Nanozymes, as nanomaterials with natural enzyme activities, have been widely applied to deliver various therapeutic agents to synergistically combat the progression of malignant tumors. However, currently common inorganic nanozyme-based drug delivery systems still face challenges such as suboptimal biosafety, inadequate stability, and inferior tumor selectivity. Herein, a super-stable amino acid-based metallo-supramolecular nanoassembly (FPIC NPs) with peroxidase (POD)- and glutathione oxidase (GSHOx)-like activities was fabricated via Pt
    MeSH term(s) Humans ; Photochemotherapy ; Amino Acids ; Combined Modality Therapy ; Indocyanine Green/pharmacology ; Neoplasms/drug therapy ; Coloring Agents ; Curcumin ; Oxidation-Reduction ; Cell Line, Tumor ; Nanoparticles
    Chemical Substances Amino Acids ; Indocyanine Green (IX6J1063HV) ; Coloring Agents ; Curcumin (IT942ZTH98)
    Language English
    Publishing date 2024-02-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241597-5
    ISSN 1095-7103 ; 0021-9797
    ISSN (online) 1095-7103
    ISSN 0021-9797
    DOI 10.1016/j.jcis.2024.02.197
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Transition Metal Dichalcogenides Nanoscrolls: Preparation and Applications.

    Yu, Shilong / Wang, Pinyi / Ye, Huihui / Tang, Hailun / Wang, Siyuan / Wu, Zhikang / Pei, Chengjie / Lu, Junhui / Li, Hai

    Nanomaterials (Basel, Switzerland)

    2023  Volume 13, Issue 17

    Abstract: Two-dimensional (2D) transition metal dichalcogenides (TMDCs) nanosheets have shown extensive applications due to their excellent physical and chemical properties. However, the low light absorption efficiency limits their application in optoelectronics. ... ...

    Abstract Two-dimensional (2D) transition metal dichalcogenides (TMDCs) nanosheets have shown extensive applications due to their excellent physical and chemical properties. However, the low light absorption efficiency limits their application in optoelectronics. By rolling up 2D TMDCs nanosheets, the one-dimensional (1D) TMDCs nanoscrolls are formed with spiral tubular structure, tunable interlayer spacing, and opening ends. Due to the increased thickness of the scroll structure, the light absorption is enhanced. Meanwhile, the rapid electron transportation is confined along the 1D structure. Therefore, the TMDCs nanoscrolls show improved optoelectronic performance compared to 2D nanosheets. In addition, the high specific surface area and active edge site from the bending strain of the basal plane make them promising materials for catalytic reaction. Thus, the TMDCs nanoscrolls have attracted intensive attention in recent years. In this review, the structure of TMDCs nanoscrolls is first demonstrated and followed by various preparation methods of the TMDCs nanoscrolls. Afterwards, the applications of TMDCs nanoscrolls in the fields of photodetection, hydrogen evolution reaction, and gas sensing are discussed.
    Language English
    Publishing date 2023-08-27
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano13172433
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Multifunctional Lithium-Doped Mesoporous Nanoparticles for Effective Dentin Regeneration in vivo.

    Liang, Zitian / Chen, Ding / Jiang, Ye / Su, Zhikang / Pi, Yixing / Luo, Tao / Jiang, Qianzhou / Yang, Li / Guo, Lvhua

    International journal of nanomedicine

    2023  Volume 18, Page(s) 5309–5325

    Abstract: Introduction: Effective infection control without irritating the pulp tissue is the key to successful vital pulp therapy. Developing a novel antibacterial biomaterial that promotes dentin regeneration for pulp capping is thus a promising strategy for ... ...

    Abstract Introduction: Effective infection control without irritating the pulp tissue is the key to successful vital pulp therapy. Developing a novel antibacterial biomaterial that promotes dentin regeneration for pulp capping is thus a promising strategy for enhancing vital pulp therapy.
    Methods: Lithium-doped mesoporous nanoparticles (Li-MNPs) were synthesized using an alkali-catalyzed sol-gel method. The particle size, elemental distribution, surface morphology, pore structure, and ion release from Li-MNPs were measured. Human dental pulp stem cells (hDPSCs) and
    Results: Li-MNPs had a larger surface area (221.18 m
    Conclusion: Li-MNPs promoted dentin regeneration and inhibited
    MeSH term(s) Humans ; Animals ; Mice ; Lithium ; Nanoparticles ; Anti-Bacterial Agents/pharmacology ; Streptococcus mutans ; Regeneration ; Dentin
    Chemical Substances Lithium (9FN79X2M3F) ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-09-18
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2364941-0
    ISSN 1178-2013 ; 1176-9114
    ISSN (online) 1178-2013
    ISSN 1176-9114
    DOI 10.2147/IJN.S424930
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Compositional variations in algal organic matter during distinct growth phases in karst water

    Liye Tian / Zhiwei Zhang / Zhikang Wang / Ping Zhang / Chao Xiong / Ye Kuang / Xingyi Peng / Mengxin Yu / Yu Qian

    Frontiers in Environmental Science, Vol

    2023  Volume 11

    Abstract: Inland surface water plays an important role in global carbon cycling, which responds to transformation between dissolved inorganic carbon (DIC) and dissolved organic carbon (DOC). Studies have shown that algae in karst lakes and reservoirs can convert ... ...

    Abstract Inland surface water plays an important role in global carbon cycling, which responds to transformation between dissolved inorganic carbon (DIC) and dissolved organic carbon (DOC). Studies have shown that algae in karst lakes and reservoirs can convert DIC to organic matter (OM) and form stable carbon sinks via photosynthesis. However, the pathways of conversion of inorganic carbon to organic carbon during algal growth remain unclear and need further investigation. In this study, spectroscopic techniques were applied to investigate the variations in algal organic matter (AOM) composition in the growth metabolism of Chlorella vulgaris and Scenedesmus obliquus under simulated karst water condition. The results showed that algal extracellular organic matter (EOM) contained high DIC concentration during the adaptation phase, which formed the carbon source for algal photosynthesis. In addition, DOC in algae increased after entering the stationary phase, while more OM was released into water. As algal growth proceeded, the amino groups in EOM were consumed to produce more aromatic protein-like material, while more lipid material was produced in intracellular organic matter (IOM). The spectral characterization results could intuitively determine AOM dynamics in different growth stages of algae, which can be used for establishing effective approaches for detecting organic carbon variations and responding to regional carbon cycling in karst water.
    Keywords algal organic matter ; karst water ; carbon cycle ; growth phases of algae ; dissolved inorganic carbon ; Environmental sciences ; GE1-350
    Subject code 550
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Improving Cleaning Performance of Rice Combine Harvesters by DEM–CFD Coupling Technology

    Ding, Bochuan / Liang, Zhenwei / Qi, Yongqi / Ye, Zhikang / Zhou, Jiahao

    Agriculture (Basel). 2022 Sept. 13, v. 12, no. 9

    2022  

    Abstract: The cleaning device is an important part of combine harvesters, as its superior or inferior performance directly affects the performance of the combine harvester greatly. With an increasing rice yield, the current single-duct cleaning performance ... ...

    Abstract The cleaning device is an important part of combine harvesters, as its superior or inferior performance directly affects the performance of the combine harvester greatly. With an increasing rice yield, the current single-duct cleaning performance declines greatly, and causes a large grain sieve loss level and a direct grain loss for the farms. To optimize the existing single-duct cleaning device to meet the large feeding rate requirement, firstly, the terminal velocity of rice grain and MOG (material other than grain) for different varieties was experimentally measured by the custom-made device. The effects of the moisture content of rice grains and the length of short straws on terminal velocity were studied in detail. Then, the gas–solid two-phase flow theory was comprehensively applied by utilizing the discrete element method (DEM) and computational fluid dynamics (CFD) to study the working mechanism of the existing single-duct cleaning unit, and the cleaning performance was evaluated from the view of the motion law of the threshing output within the cleaning shoe. At last, a multi-duct cleaning device was put forward, and a field experiment was performed to assess the performance of the newly developed cleaning device. The results showed that the grain sieve losses ratio and grain impurity ratio improved dramatically, proving that structure optimization of the cleaning device was feasible.
    Keywords agriculture ; combine harvesters ; field experimentation ; fluid mechanics ; rice ; terminal velocity ; water content
    Language English
    Dates of publication 2022-0913
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2651678-0
    ISSN 2077-0472
    ISSN 2077-0472
    DOI 10.3390/agriculture12091457
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Decreased hemoglobin as a quantifiable indicator of renal arterial embolization in post-percutaneous nephrolithotomy hemorrhage.

    Ran, Ruitu / Zhang, Ruiyuan / Xie, Ye / Yin, Zhikang

    Urolithiasis

    2020  Volume 49, Issue 2, Page(s) 137–143

    Abstract: To determine quantifiable indicators for post-percutaneous nephrolithotomy (PCNL) renal arterial embolization. A total of 2043 patients who underwent PCNL from September 2012 to March 2018 were reviewed retrospectively. Post-operative hemorrhage patients ...

    Abstract To determine quantifiable indicators for post-percutaneous nephrolithotomy (PCNL) renal arterial embolization. A total of 2043 patients who underwent PCNL from September 2012 to March 2018 were reviewed retrospectively. Post-operative hemorrhage patients were extracted and divided into two groups according to treatment methods (conservative methods or super-selective renal arterial embolization [SRAE]). Demographic characteristics and hemorrhage outcomes were compared between the two groups by univariable analysis. Multivariable logistic regression was used to reveal the association between hemorrhage outcome factors and SRAE. A receiver operating characteristic (ROC) curve was drawn to determine the optimized cut-off value for SRAE. We identified 71 patients who had post-PCNL hemorrhage. Seventeen and 54 patients comprised the SRAE and conservative groups, respectively. No significant differences in demographic characteristics were found between the two groups. Univariate analysis showed that the differences in decreased hemoglobin (Hb), hemorrhage types, and transfusion were significant between the two groups (p < 0.001). Multivariable analysis showed that the decreased Hb was closely associated with the risk of SRAE. The ROC curve showed that an adjusted Hb decrease of 3.45 g/dL was an optimum indicator (AUC = 0.925). Decreased Hb is an indicator for SRAE after PCNL. When the adjusted decrease in Hb is ≥ 3.45 g/dL, SRAE should be performed regardless of the manifestations of hemorrhage.
    MeSH term(s) Adult ; Aged ; Blood Transfusion/statistics & numerical data ; Conservative Treatment/statistics & numerical data ; Embolization, Therapeutic/statistics & numerical data ; Female ; Hemoglobins/analysis ; Humans ; Kidney Calculi/surgery ; Male ; Middle Aged ; Nephrolithotomy, Percutaneous/adverse effects ; Postoperative Hemorrhage/blood ; Postoperative Hemorrhage/diagnosis ; Postoperative Hemorrhage/therapy ; Renal Artery/surgery ; Retrospective Studies ; Risk Assessment/statistics & numerical data ; Severity of Illness Index ; Treatment Outcome
    Chemical Substances Hemoglobins
    Language English
    Publishing date 2020-08-08
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2703553-0
    ISSN 2194-7236 ; 2194-7228
    ISSN (online) 2194-7236
    ISSN 2194-7228
    DOI 10.1007/s00240-020-01206-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: A four-quadrant conceptual framework for analyzing extended producer responsibility in offshore prefabrication construction.

    Xu, Jinying / Ye, Meng / Lu, Weisheng / Bao, Zhikang / Webster, Chris

    Journal of cleaner production

    2020  Volume 282, Page(s) 124540

    Abstract: Prefabrication has been widely advocated as a green production strategy to minimize the adverse environmental impacts of construction. Amid economic globalization, prefabricated construction materials are commonly sourced offsite and even offshore. As an ...

    Abstract Prefabrication has been widely advocated as a green production strategy to minimize the adverse environmental impacts of construction. Amid economic globalization, prefabricated construction materials are commonly sourced offsite and even offshore. As an issue emerging alongside offshore prefabrication, extended producer responsibility (EPR) is yet to be clearly identified, allocated, and implemented. This research develops a conceptual framework using a design thinking process, through which EPR associated with offshore prefabrication can be analyzed, agreed upon, and allocated. By considering the scope and scale of the responsibility and the procurement methods, the framework comprises four quadrants representing four typical scenarios for implementation of the EPR principle. It is applicable for both short-term and lifelong EPR analysis, in both traditional and integrated project delivery contexts. The framework will be particularly useful for devising public policies to achieve an onshore and offshore stakeholder win-win situation.
    Keywords covid19
    Language English
    Publishing date 2020-10-07
    Publishing country Netherlands
    Document type Journal Article
    ISSN 0959-6526
    ISSN 0959-6526
    DOI 10.1016/j.jclepro.2020.124540
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Book ; Online: CrowdCLIP

    Liang, Dingkang / Xie, Jiahao / Zou, Zhikang / Ye, Xiaoqing / Xu, Wei / Bai, Xiang

    Unsupervised Crowd Counting via Vision-Language Model

    2023  

    Abstract: Supervised crowd counting relies heavily on costly manual labeling, which is difficult and expensive, especially in dense scenes. To alleviate the problem, we propose a novel unsupervised framework for crowd counting, named CrowdCLIP. The core idea is ... ...

    Abstract Supervised crowd counting relies heavily on costly manual labeling, which is difficult and expensive, especially in dense scenes. To alleviate the problem, we propose a novel unsupervised framework for crowd counting, named CrowdCLIP. The core idea is built on two observations: 1) the recent contrastive pre-trained vision-language model (CLIP) has presented impressive performance on various downstream tasks; 2) there is a natural mapping between crowd patches and count text. To the best of our knowledge, CrowdCLIP is the first to investigate the vision language knowledge to solve the counting problem. Specifically, in the training stage, we exploit the multi-modal ranking loss by constructing ranking text prompts to match the size-sorted crowd patches to guide the image encoder learning. In the testing stage, to deal with the diversity of image patches, we propose a simple yet effective progressive filtering strategy to first select the highly potential crowd patches and then map them into the language space with various counting intervals. Extensive experiments on five challenging datasets demonstrate that the proposed CrowdCLIP achieves superior performance compared to previous unsupervised state-of-the-art counting methods. Notably, CrowdCLIP even surpasses some popular fully-supervised methods under the cross-dataset setting. The source code will be available at https://github.com/dk-liang/CrowdCLIP.

    Comment: Accepted by CVPR 2023
    Keywords Computer Science - Computer Vision and Pattern Recognition
    Subject code 004
    Publishing date 2023-04-09
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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