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  1. Article ; Online: Freeing up Japan's PhD potential.

    Hoshino, Ayuko

    Nature

    2023  Volume 615, Issue 7951, Page(s) S57

    MeSH term(s) Education, Graduate ; Japan ; Research Personnel/education ; Research Personnel/standards ; Research Personnel/supply & distribution
    Language English
    Publishing date 2023-02-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/d41586-023-00659-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 26: Show issues Location:
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  2. Article ; Online: Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification.

    Kenari, Amirmohammad Nasiri / Bojmar, Linda / Heissel, Søren / Molina, Henrik / Lyden, David / Hoshino, Ayuko

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2628, Page(s) 291–300

    Abstract: Plasma extracellular vesicles and particles (EVPs) are enriched in biomolecules that reflect individuals' physiological and pathological states. Several studies have demonstrated the potential of human plasma EVPs as a novel liquid biopsy. Here we ... ...

    Abstract Plasma extracellular vesicles and particles (EVPs) are enriched in biomolecules that reflect individuals' physiological and pathological states. Several studies have demonstrated the potential of human plasma EVPs as a novel liquid biopsy. Here we describe a protocol for human plasma EVPs isolation and proteomic characterization. We isolated human plasma EVPs by the classical ultracentrifugation method and performed mass spectrometry-based proteomic profiling. Using this protocol, researchers can reveal the plasma EVPs proteome and explore the clinical application of plasma EVPs proteins for developing disease biomarkers.
    MeSH term(s) Humans ; Proteomics/methods ; Mass Spectrometry ; Ultracentrifugation ; Blood Proteins/metabolism ; Extracellular Vesicles/metabolism ; Proteome/metabolism
    Chemical Substances Blood Proteins ; Proteome
    Language English
    Publishing date 2023-02-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2978-9_19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: [Exosome, a Nano-Sized Carrier, and the Brain-Gut Correlation].

    Sugiura, Kei / Shimura, Hinako / Hoshino, Ayuko

    Brain and nerve = Shinkei kenkyu no shinpo

    2021  Volume 73, Issue 8, Page(s) 879–887

    Abstract: Exosomes, small bilayer vesicles secreted by all cells, are recognized as a novel communication tool among distant organs. They can cross the blood-brain barrier and be taken up by specific brain cells, altering the brain microenvironment and possibly ... ...

    Abstract Exosomes, small bilayer vesicles secreted by all cells, are recognized as a novel communication tool among distant organs. They can cross the blood-brain barrier and be taken up by specific brain cells, altering the brain microenvironment and possibly playing a role in pathological conditions such as brain metastasis and neurodegenerative diseases. Intestinal bacteria also release small membrane vesicles called "bacterial extracellular vesicles" (BEVs), which can invade blood vessels and affect distant tissues in a manner similar to exosomes, suggesting that BEVs also play a role in disease progression in the brain-gut axis. Here, we will discuss the latest findings of the relationship between exosomes, including BEVs, and multiple brain pathologies.
    MeSH term(s) Brain ; Disease Progression ; Exosomes ; Extracellular Vesicles ; Humans
    Language Japanese
    Publishing date 2021-08-10
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 390389-8
    ISSN 1344-8129 ; 1881-6096 ; 0006-8969
    ISSN (online) 1344-8129
    ISSN 1881-6096 ; 0006-8969
    DOI 10.11477/mf.1416201855
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Metastasis: Lymphatic detours for cancer.

    Hoshino, Ayuko / Lyden, David

    Nature

    2017  Volume 546, Issue 7660, Page(s) 609–610

    MeSH term(s) Humans ; Lymphatic Metastasis ; Lymphatic Vessels ; Neoplasms
    Language English
    Publishing date 2017--28
    Publishing country England
    Document type News ; Comment
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/546609a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Protocol for cross-platform characterization of human and murine extracellular vesicles and particles

    Linda Bojmar / Han Sang Kim / Kei Sugiura / Søren Heissel / Serena Lucotti / Michele Cioffi / Kofi Ennu Johnson / Leona Cohen-Gould / Haiying Zhang / Henrik Molina / Irina R. Matei / David Lyden / Ayuko Hoshino

    STAR Protocols, Vol 5, Iss 1, Pp 102754- (2024)

    1481  

    Abstract: ... of this protocol, please refer to Hoshino et al.1 : Publisher’s note: Undertaking any experimental protocol ...

    Abstract Summary: Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, we present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mice. We describe steps for nanoparticle tracking analysis, transmission electron microscopy, single-EVP immunodetection, EVP proteomic mass spectrometry and bioinformatic analysis, and EVP protein validation by ExoELISA and western blot analysis. This allows for EVP cross-validation across different platforms.For complete details on the use and execution of this protocol, please refer to Hoshino et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
    Keywords Cell Biology ; Cell isolation ; Cell Membrane ; Model Organisms ; Molecular Biology ; Protein Biochemistry ; Science (General) ; Q1-390
    Language English
    Publishing date 2024-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: A peptide binding to the tetraspanin CD9 reduces cancer metastasis.

    Suwatthanarak, Thanawat / Ito, Kazuma / Tanaka, Masayoshi / Sugiura, Kei / Hoshino, Ayuko / Miyamoto, Yoshitaka / Miyado, Kenji / Okochi, Mina

    Biomaterials advances

    2023  Volume 146, Page(s) 213283

    Abstract: As an organizer of multi-molecular membrane complexes, the tetraspanin CD9 has been implicated in a number of biological processes, including cancer metastasis, and is a candidate therapeutic target. Here, we evaluated the suppressive effects of an eight- ...

    Abstract As an organizer of multi-molecular membrane complexes, the tetraspanin CD9 has been implicated in a number of biological processes, including cancer metastasis, and is a candidate therapeutic target. Here, we evaluated the suppressive effects of an eight-mer CD9-binding peptide (CD9-BP) on cancer cell metastasis and its mechanisms of action. CD9-BP impaired CD9-related functions by adversely affecting the formation of tetraspanin webs-networks composed of CD9 and its partner proteins. The anti-cancer metastasis effect of CD9-BP was evidenced by the in vitro inhibition of cancer cell migration and invasion as well as exosome secretion and uptake, which are essential processes during metastasis. Finally, using a mouse model, we showed that CD9-BP reduced lung metastasis in vivo. These findings provide insight into the mechanism by which CD9-BP inhibits CD9-dependent functions and highlight its potential application as an alternative therapeutic nano-biomaterial for metastatic cancers.
    MeSH term(s) Humans ; Neoplasms/pathology ; Neoplasms/therapy ; Tetraspanin 29/metabolism ; Neoplasm Metastasis ; Oligopeptides/metabolism ; Oligopeptides/therapeutic use
    Chemical Substances CD9 protein, human ; Tetraspanin 29 ; Oligopeptides
    Language English
    Publishing date 2023-01-06
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2772-9508
    ISSN (online) 2772-9508
    DOI 10.1016/j.bioadv.2023.213283
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  7. Article ; Online: Impact of exosome-mediated feto-maternal interactions on pregnancy maintenance and development of obstetric complications.

    Hashimoto, Ayako / Sugiura, Kei / Hoshino, Ayuko

    Journal of biochemistry

    2020  Volume 169, Issue 2, Page(s) 163–171

    Abstract: Pregnancy is an immunological paradox, a phenomenon in which the foetus and the placenta, containing foreign antigens to the mother, develop without inducing rejection by the maternal immune system. Cell-to-cell communication between the foetus and the ... ...

    Abstract Pregnancy is an immunological paradox, a phenomenon in which the foetus and the placenta, containing foreign antigens to the mother, develop without inducing rejection by the maternal immune system. Cell-to-cell communication between the foetus and the mother is mediated by secreted factors such as cytokines, hormones and extracellular vesicles (EVs) for a successful pregnancy and to avoid rejection. Exosomes, the smallest of EVs, are released extracellularly, where they are taken up by proximal or distant recipient cells. Here, we discuss the role of EVs, especially exosomes in feto-maternal communication during pregnancy. This review will provide an overview of the functional roles exosomes may play during embryo implantation, modulating immune responses during pregnancy and the onset of labour. Moreover, we will discuss exosomal function in obstetric pathology, and the development of pregnancy-associated complications such as preeclampsia and preterm birth as well as the biomarker potential of exosomes for detecting such conditions.
    MeSH term(s) Biomarkers/metabolism ; Exosomes/physiology ; Extracellular Vesicles/immunology ; Extracellular Vesicles/metabolism ; Extracellular Vesicles/pathology ; Female ; Humans ; Maternal-Fetal Relations/physiology ; Placenta/physiology ; Pregnancy ; Pregnancy Complications/immunology ; Pregnancy Complications/metabolism ; Pregnancy Complications/pathology ; Pregnancy Maintenance/physiology ; Premature Birth
    Chemical Substances Biomarkers
    Language English
    Publishing date 2020-11-24
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 218073-x
    ISSN 1756-2651 ; 0021-924X
    ISSN (online) 1756-2651
    ISSN 0021-924X
    DOI 10.1093/jb/mvaa137
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Uc I Zs.202: Show issues Location:
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  8. Article ; Online: GeLC-FAIMS-MS workflow for in-depth middle-down proteomics.

    Takemori, Ayako / Kaulich, Philipp T / Konno, Ryo / Kawashima, Yusuke / Hamazaki, Yuto / Hoshino, Ayuko / Tholey, Andreas / Takemori, Nobuaki

    Proteomics

    2023  Volume 24, Issue 3-4, Page(s) e2200431

    Abstract: Middle-down proteomics (MDP) is an analytical approach in which protein samples are digested with proteases such as Glu-C to generate large peptides (>3 kDa) that are analyzed by mass spectrometry (MS). This method is useful for characterizing high- ... ...

    Abstract Middle-down proteomics (MDP) is an analytical approach in which protein samples are digested with proteases such as Glu-C to generate large peptides (>3 kDa) that are analyzed by mass spectrometry (MS). This method is useful for characterizing high-molecular-weight proteins that are difficult to detect by top-down proteomics (TDP), in which intact proteins are analyzed by MS. In this study, we applied GeLC-FAIMS-MS, a multidimensional separation workflow that combines gel-based prefractionation with LC-FAIMS MS, for deep MDP. Middle-down peptides generated by optimized limited Glu-C digestion conditions were first size-fractionated by polyacrylamide gel electrophoresis, followed by C4 reversed-phase liquid chromatography separation and additional ion mobility fractionation, resulting in a significant increase in peptide length detectable by MS. In addition to global analysis, the GeLC-FAIMS-MS concept can also be applied to targeted MDP, where only proteins in the desired molecular weight range are gel-fractionated and their Glu-C digestion products are analyzed, as demonstrated by targeted analysis of integrins in exosomes. In-depth MDP achieved by global and targeted GeLC-FAIMS-MS supports the exploration of proteoform information not covered by conventional TDP by increasing the number of detectable protein groups or post-translational modifications (PTMs) and improving the sequence coverage.
    MeSH term(s) Proteomics/methods ; Workflow ; Tandem Mass Spectrometry ; Peptides/analysis ; DNA-Binding Proteins
    Chemical Substances Peptides ; DNA-Binding Proteins
    Language English
    Publishing date 2023-08-07
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2032093-0
    ISSN 1615-9861 ; 1615-9853
    ISSN (online) 1615-9861
    ISSN 1615-9853
    DOI 10.1002/pmic.202200431
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  9. Article: Advancement in Cellular Topographic and Nanoparticle Capture Imaging by High Resolution Microscopy Incorporating a Freeze-Drying and Gaseous Nitrogen-based Approach.

    Uryu, Kunihiro / Soplop, Nadine / Sheahan, Timothy P / Catanese, Maria-Teresa / Huynh, Chuong / Pena, John / Boudreau, Nancy / Matei, Irina / Kenific, Candia / Hashimoto, Ayako / Hoshino, Ayuko / Rice, Charles M / Lyden, David

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Scanning electron microscopy (SEM) offers an unparalleled view of the membrane topography of mammalian cells by using a conventional osmium ( ... ...

    Abstract Scanning electron microscopy (SEM) offers an unparalleled view of the membrane topography of mammalian cells by using a conventional osmium (OsO
    Language English
    Publishing date 2023-09-28
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.09.28.559906
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Protocol for cross-platform characterization of human and murine extracellular vesicles and particles.

    Bojmar, Linda / Kim, Han Sang / Sugiura, Kei / Heissel, Søren / Lucotti, Serena / Cioffi, Michele / Johnson, Kofi Ennu / Cohen-Gould, Leona / Zhang, Haiying / Molina, Henrik / Matei, Irina R / Lyden, David / Hoshino, Ayuko

    STAR protocols

    2023  Volume 5, Issue 1, Page(s) 102754

    Abstract: ... of this protocol, please refer to Hoshino et al. ...

    Abstract Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, we present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mice. We describe steps for nanoparticle tracking analysis, transmission electron microscopy, single-EVP immunodetection, EVP proteomic mass spectrometry and bioinformatic analysis, and EVP protein validation by ExoELISA and western blot analysis. This allows for EVP cross-validation across different platforms. For complete details on the use and execution of this protocol, please refer to Hoshino et al.
    MeSH term(s) Humans ; Animals ; Mice ; Proteomics ; Blotting, Western ; Computational Biology ; Extracellular Vesicles ; Mass Spectrometry
    Language English
    Publishing date 2023-12-13
    Publishing country United States
    Document type Journal Article
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2023.102754
    Database MEDical Literature Analysis and Retrieval System OnLINE

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