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Article ; Online: On the road to synthetic life: the minimal cell and genome-scale engineering.

Juhas, Mario

2016 Volume 36, Issue 3, Page(s) 416–423

Abstract: Synthetic biology employs rational engineering principles to build biological systems from the libraries of standard, well characterized biological parts. Biological systems designed and built by synthetic biologists fulfill a plethora of useful purposes, ...

Abstract	Synthetic biology employs rational engineering principles to build biological systems from the libraries of standard, well characterized biological parts. Biological systems designed and built by synthetic biologists fulfill a plethora of useful purposes, ranging from better healthcare and energy production to biomanufacturing. Recent advancements in the synthesis, assembly and "booting-up" of synthetic genomes and in low and high-throughput genome engineering have paved the way for engineering on the genome-wide scale. One of the key goals of genome engineering is the construction of minimal genomes consisting solely of essential genes (genes indispensable for survival of living organisms). Besides serving as a toolbox to understand the universal principles of life, the cell encoded by minimal genome could be used to build a stringently controlled "cell factory" with a desired phenotype. This review provides an update on recent advances in the genome-scale engineering with particular emphasis on the engineering of minimal genomes. Furthermore, it presents an ongoing discussion to the scientific community for better suitability of minimal or robust cells for industrial applications.
MeSH term(s)	Biotechnology ; Genetic Engineering ; Synthetic Biology
Language	English
Publishing date	2016
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1042364-3
ISSN	1549-7801 ; 0738-8551
ISSN (online)	1549-7801
ISSN	0738-8551
DOI	10.3109/07388551.2014.989423
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Zs.A 2554: Show issues

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Article ; Online: Fluorescence detection of the human angiotensinogen protein by the G-quadruplex aptamer.

Xi, Hui / Jiang, Hanlin / Juhas, Mario / Zhang, Yang

The Analyst

2022 Volume 147, Issue 18, Page(s) 4040–4048

Abstract: Noncanonical G-quadruplex nucleic acid structures can be used as probes in biosensors for the detection of metal ions, proteins and nucleic acids. Angiotensinogen (AGT) is a glycosylated globulin found in serum, which can regulate blood pressure and body ...

Abstract	Noncanonical G-quadruplex nucleic acid structures can be used as probes in biosensors for the detection of metal ions, proteins and nucleic acids. Angiotensinogen (AGT) is a glycosylated globulin found in serum, which can regulate blood pressure and body fluid homeostasis. AGT is an important part of the renin-angiotensin system (RAS) and can be potentially used as a biomarker of diseases with RAS alterations. G-quadruplex based biosensors can detect targets with high accuracy and speed and low cost. Employing the magnetic bead enrichment method we constructed a reliable and efficient fluorescent biosensor platform for G-quadruplex based detection of the human AGT protein. The primary antibody in our biosensor is recognized by fluorescently labeled secondary antibodies, which leads to the detection of AGT captured by the G-quadruplex aptamer coupled magnetic beads. This G-quadruplex based fluorescent biosensor designed with a detection limit of 5 × 10
MeSH term(s)	Angiotensinogen/metabolism ; Aptamers, Nucleotide/chemistry ; Biosensing Techniques/methods ; G-Quadruplexes ; Humans ; Spectrometry, Fluorescence
Chemical Substances	Aptamers, Nucleotide ; Angiotensinogen (11002-13-4)
Language	English
Publishing date	2022-09-12
Publishing country	England
Document type	Journal Article
ZDB-ID	210747-8
ISSN	1364-5528 ; 0003-2654
ISSN (online)	1364-5528
ISSN	0003-2654
DOI	10.1039/d2an01057g
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Zs.A 2423: Show issues

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Article ; Online: Aptamers targeting SARS-COV-2: a promising tool to fight against COVID-19.

Zhang, Yang / Juhas, Mario / Kwok, Chun Kit

Trends in biotechnology

2022 Volume 41, Issue 4, Page(s) 528–544

Abstract: SARS-CoV-2, the causative agent of COVID-19, remains among the main causes of global mortality. Although antigen/antibody-based immunoassays and neutralizing antibodies targeting SARS-CoV-2 have been successfully developed over the past 2 years, they are ...

Abstract	SARS-CoV-2, the causative agent of COVID-19, remains among the main causes of global mortality. Although antigen/antibody-based immunoassays and neutralizing antibodies targeting SARS-CoV-2 have been successfully developed over the past 2 years, they are often inefficient and unreliable for emerging SARS-CoV-2 variants. Novel approaches against SARS-CoV-2 and its variants are therefore urgently needed. Aptamers have been developed for the detection and inhibition of several different viruses such as HIV, influenza viruses, Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV. Aptamers targeting SARS-CoV-2 represent a promising tool in the fight against COVID-19, which is of paramount importance for the current and any future pandemics. This review presents recent advances and future trends in the development of aptamer-based approaches for SARS-CoV-2 diagnosis and treatment.
MeSH term(s)	Humans ; COVID-19/diagnosis ; SARS-CoV-2 ; COVID-19 Testing ; Middle East Respiratory Syndrome Coronavirus
Language	English
Publishing date	2022-08-01
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	47474-5
ISSN	1879-3096 ; 0167-7799
ISSN (online)	1879-3096
ISSN	0167-7799
DOI	10.1016/j.tibtech.2022.07.012
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Zs.A 2750: Show issues

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Article: Biosensors for Point Mutation Detection.

Jiang, Hanlin / Xi, Hui / Juhas, Mario / Zhang, Yang

Frontiers in bioengineering and biotechnology

2021 Volume 9, Page(s) 797831

Language	English
Publishing date	2021-12-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2021.797831
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Article ; Online: Horizontal gene transfer in human pathogens.

Juhas, Mario

Critical reviews in microbiology

2015 Volume 41, Issue 1, Page(s) 101–108

Abstract: Horizontal gene transfer has a tremendous impact on the genome plasticity, adaptation and evolution of bacteria. Horizontally transferred mobile genetic elements are involved in the dissemination of antibiotic resistance and virulence genes, thus ... ...

Abstract	Horizontal gene transfer has a tremendous impact on the genome plasticity, adaptation and evolution of bacteria. Horizontally transferred mobile genetic elements are involved in the dissemination of antibiotic resistance and virulence genes, thus contributing to the emergence of novel "superbugs". This review provides update on various mechanisms of horizontal gene transfer and examines how horizontal gene transfer contributes to the evolution of pathogenic bacteria. Special focus is paid to the role horizontal gene transfer plays in pathogenicity of the emerging human pathogens: hypervirulent Clostridium difficile and Escherichia coli (including the most recent haemolytic uraemic syndrome outbreak strain) and methicillin-resistant Staphylococcus aureus (MRSA), which have been associated with largest outbreaks of infection recently.
MeSH term(s)	Bacterial Infections/microbiology ; Bacterial Physiological Phenomena ; Clostridium difficile/genetics ; Clostridium difficile/pathogenicity ; Escherichia coli/genetics ; Escherichia coli/pathogenicity ; Gene Transfer, Horizontal ; Humans ; Virulence
Language	English
Publishing date	2015-02
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1053620-6
ISSN	1549-7828 ; 1040-841X
ISSN (online)	1549-7828
ISSN	1040-841X
DOI	10.3109/1040841X.2013.804031
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Zs.A 1025: Show issues

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Article ; Online: Pseudomonas aeruginosa essentials: an update on investigation of essential genes.

Juhas, Mario

Microbiology (Reading, England)

2015 Volume 161, Issue 11, Page(s) 2053–2060

Abstract: Pseudomonas aeruginosa is the leading cause of nosocomial infections, particularly in immunocompromised, cancer, burn and cystic fibrosis patients. Development of novel antimicrobials against P. aeruginosa is therefore of the highest importance. Although ...

Abstract	Pseudomonas aeruginosa is the leading cause of nosocomial infections, particularly in immunocompromised, cancer, burn and cystic fibrosis patients. Development of novel antimicrobials against P. aeruginosa is therefore of the highest importance. Although the first reports on P. aeruginosa essential genes date back to the early 2000s, a number of more sensitive genomic approaches have been used recently to better define essential genes in this organism. These analyses highlight the evolution of the definition of an 'essential' gene from the traditional to the context-dependent. Essential genes, particularly those indispensable under the clinically relevant conditions, are considered to be promising targets of novel antibiotics against P. aeruginosa. This review provides an update on the investigation of P. aeruginosa essential genes. Special focus is on recently identified P. aeruginosa essential genes and their exploitation for the development of antimicrobials.
MeSH term(s)	Anti-Bacterial Agents/isolation & purification ; Anti-Bacterial Agents/pharmacology ; Drug Discovery ; Genes, Essential ; Humans ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2015-08-24
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1180712-x
ISSN	1465-2080 ; 1350-0872
ISSN (online)	1465-2080
ISSN	1350-0872
DOI	10.1099/mic.0.000161
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Ud II Zs.140: Show issues

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Article ; Online: Type IV secretion systems and genomic islands-mediated horizontal gene transfer in Pseudomonas and Haemophilus.

Juhas, Mario

Microbiological research

2015 Volume 170, Page(s) 10–17

Abstract: Bacterial secretion systems, such as type IV secretion systems (T4SSs) are multi-subunit machines transferring macromolecules across membranes. Besides proteins, T4SSs also transfer nucleoprotein complexes, thus having a significant impact on the ... ...

Abstract	Bacterial secretion systems, such as type IV secretion systems (T4SSs) are multi-subunit machines transferring macromolecules across membranes. Besides proteins, T4SSs also transfer nucleoprotein complexes, thus having a significant impact on the evolution of bacterial species. By T4SS-mediated horizontal gene transfer bacteria can acquire a broad spectrum of fitness genes allowing them to thrive in the wide variety of environments. Furthermore, acquisition of antibiotic-resistance and virulence genes can lead to the emergence of novel 'superbugs'. This review provides an update on the investigation of T4SSs. It highlights the role T4SSs play in the horizontal gene transfer, particularly in the evolution of catabolic pathways, antibiotic-resistance and virulence in Haemophilus and Pseudomonas.
MeSH term(s)	Bacterial Secretion Systems ; Drug Resistance, Neoplasm/genetics ; Gene Transfer, Horizontal ; Genomic Islands ; Haemophilus/genetics ; Haemophilus/metabolism ; Pseudomonas/genetics ; Pseudomonas/metabolism
Chemical Substances	Bacterial Secretion Systems
Language	English
Publishing date	2015-01
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1189614-0
ISSN	1618-0623 ; 0944-5013
ISSN (online)	1618-0623
ISSN	0944-5013
DOI	10.1016/j.micres.2014.06.007
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Ud II Zs.7: Show issues

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Article ; Online: Deep Learning for Imaging and Detection of Microorganisms.

Zhang, Yang / Jiang, Hao / Ye, Taoyu / Juhas, Mario

Trends in microbiology

2021 Volume 29, Issue 7, Page(s) 569–572

Abstract: Despite tremendous recent interest, the application of deep learning in microbiology has still not reached its full potential. To tackle the challenges faced by human-operated microscopy, deep-learning-based methods have been proposed for microscopic ... ...

Abstract	Despite tremendous recent interest, the application of deep learning in microbiology has still not reached its full potential. To tackle the challenges faced by human-operated microscopy, deep-learning-based methods have been proposed for microscopic image analysis of a wide range of microorganisms, including viruses, bacteria, fungi, and parasites. We believe that deep-learning technology-based systems will be on the front line of monitoring and investigation of microorganisms.
MeSH term(s)	Communicable Diseases/diagnostic imaging ; Communicable Diseases/microbiology ; Communicable Diseases/parasitology ; Communicable Diseases/virology ; Deep Learning ; Humans ; Image Processing, Computer-Assisted/methods ; Microscopy/instrumentation ; Microscopy/methods
Language	English
Publishing date	2021-01-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1158963-2
ISSN	1878-4380 ; 0966-842X
ISSN (online)	1878-4380
ISSN	0966-842X
DOI	10.1016/j.tim.2021.01.006
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Zs.A 3738: Show issues

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Article ; Online: KPC-Mediated Resistance to Ceftazidime-Avibactam and Collateral Effects in Klebsiella pneumoniae.

Findlay, Jacqueline / Poirel, Laurent / Juhas, Mario / Nordmann, Patrice

Antimicrobial agents and chemotherapy

2021 Volume 65, Issue 9, Page(s) e0089021

Abstract: Carbapenem- ... ...

Abstract	Carbapenem-resistant
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Azabicyclo Compounds/pharmacology ; Bacterial Proteins/genetics ; Ceftazidime/pharmacology ; Drug Combinations ; Humans ; Klebsiella Infections/drug therapy ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; Azabicyclo Compounds ; Bacterial Proteins ; Drug Combinations ; avibactam, ceftazidime drug combination ; Ceftazidime (9M416Z9QNR) ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
Language	English
Publishing date	2021-08-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.00890-21
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Zs.A 809: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Einzelsignatur: Show issues

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Article ; Online: Multiplex Biosensing for Simultaneous Detection of Mutations in SARS-CoV-2.

Xi, Hui / Jiang, Hanlin / Juhas, Mario / Zhang, Yang

ACS omega

2021 Volume 6, Issue 40, Page(s) 25846–25859

Abstract: COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) has become the world's largest public health emergency of the past few decades. Thousands of mutations were identified in the SARS-CoV-2 genome. Some ... ...

Abstract	COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) has become the world's largest public health emergency of the past few decades. Thousands of mutations were identified in the SARS-CoV-2 genome. Some mutants are more infectious and may replace the original strains. Recently, B.1.1.7(Alpha), B1.351(Beta), and B.1.617.2(Delta) strains, which appear to have increased transmissibility, were detected. These strains accounting for the high proportion of newly diagnosed cases spread rapidly over the world. Particularly, the Delta variant has been reported to account for a vast majority of the infections in several countries over the last few weeks. The application of biosensors in the detection of SARS-CoV-2 is important for the control of the COVID-19 pandemic. Due to high demand for SARS-CoV-2 genotyping, it is urgent to develop reliable and efficient systems based on integrated multiple biosensor technology for rapid detection of multiple SARS-CoV-2 mutations simultaneously. This is important not only for the detection and analysis of the current but also for future mutations. Novel biosensors combined with other technologies can be used for the reliable and effective detection of SARS-CoV-2 mutants.
Language	English
Publishing date	2021-09-28
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.1c04024
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