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Book ; Online: Tópicos em Virologia

Lemos, Elba Regina Sampaio de / Villar, Livia Melo / Leon, Luciane Almeida Amado / Guimarães, Monick Lindenmeyer / Teixeira, Sylvia Lopes Maia / Paula, Vanessa Salete de

(Bio)

2023

Series title	Bio
Keywords	Infectious & contagious diseases ; Infectious and contagious diseases
Language	Portuguese
Size	1 electronic resource (300 pages)
Publisher	SciELO Books - Coleção Bio \| Editora Fiocruz
Document type	Book ; Online
Note	Portuguese
HBZ-ID	HT030383592
ISBN	9786557081518 ; 6557081519
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Seroprevalence of anti-SARS-CoV-2 in patients with hepatitis B and C: a pre-vaccination study.

da Silva, Lucas Lima / Lewis-Ximenez, Lia Laura / Figueiredo, Monica Magalhaes / de Paula, Vanessa Salete / Villar, Livia Melo

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

2024 , Page(s) 103740

Abstract: The serological markers for the diagnosis of COVID-19 plays an important role in the epidemiological investigation of the pandemic. This study aims to assess the prevalence of anti-SARS-CoV-2 in hepatitis B and C patients in a pre-vaccination of COVID-19 ...

Abstract	The serological markers for the diagnosis of COVID-19 plays an important role in the epidemiological investigation of the pandemic. This study aims to assess the prevalence of anti-SARS-CoV-2 in hepatitis B and C patients in a pre-vaccination of COVID-19 period. Between March 2020 and January 2021, 199 serum samples from individuals with HBsAg/HBV DNA or anti-HCV/HCV RNA positivity were tested for antibodies (IgM and IgG) against SARS-CoV-2 using Electrochemiluminescent Immunoassay (ECLIA). Among these, 50.3 % (100/199) tested positive for hepatitis C virus infection and 49.7 % (99/199) for hepatitis B virus, confirmed through molecular and serological diagnosis. The anti-SARS-CoV-2 seroprevalence was 24.1 % (48/199) in this population, with 23.23 % (23/99) hepatitis B and 25 % (25/100) hepatitis C patients tested positive for anti-SARS-CoV-2. The higher seroprevalence of anti-SARS-CoV-2 (16.58 %, 33/199) was detected among those over-40 years of age and the month of November 2020 had the highest number of detections 9 % (18/199) with the majority living in impoverished and neglected neighborhoods in the city of Rio de Janeiro. We found a high prevalence of anti-SARS-CoV-2 in patients with viral hepatitis before COVID-19 vaccination. This demonstrates the high exposure of this population during the period of social isolation.
Language	English
Publishing date	2024-04-23
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2041400-6
ISSN	1678-4391 ; 1413-8670
ISSN (online)	1678-4391
ISSN	1413-8670
DOI	10.1016/j.bjid.2024.103740
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Article ; Online: Comparison of four extraction methods for the detection of hepatitis B virus DNA in dried blood spot samples.

Bezerra, Cristianne Sousa / Portilho, Moyra Machado / Frota, Cristiane Cunha / Villar, Lívia Melo

MicrobiologyOpen

2021 Volume 10, Issue 2, Page(s) e1161

Abstract: The dried blood spot (DBS) samples are a useful resource for viral DNA isolation and important in increasing access to HBV diagnosis. However, the choice of the DNA extraction method is crucial for reliable results. We compared the reliability of four ... ...

Abstract	The dried blood spot (DBS) samples are a useful resource for viral DNA isolation and important in increasing access to HBV diagnosis. However, the choice of the DNA extraction method is crucial for reliable results. We compared the reliability of four DNA extraction methods using DBS samples for the qualitative and quantitative detection of HBV. A panel of serially diluted HBV DNA in whole blood was spotted onto filter paper (Whatman 903 paper and Whatman FTA cards). Four methods were used to extract DNA: QIAamp
MeSH term(s)	DNA, Viral/isolation & purification ; Diagnostic Tests, Routine/methods ; Dried Blood Spot Testing/methods ; Genes, Viral ; Hepatitis B/diagnosis ; Hepatitis B/virology ; Hepatitis B virus/classification ; Hepatitis B virus/genetics ; Humans ; Molecular Diagnostic Techniques/methods ; Real-Time Polymerase Chain Reaction/methods ; Reproducibility of Results ; Sensitivity and Specificity ; Specimen Handling/methods
Chemical Substances	DNA, Viral
Language	English
Publishing date	2021-05-10
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661368-2
ISSN	2045-8827 ; 2045-8827
ISSN (online)	2045-8827
ISSN	2045-8827
DOI	10.1002/mbo3.1161
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Article ; Online: Hepatitis E Prevalence in Vulnerable Populations in Goiânia, Central Brazil.

Teles, Sheila Araújo / Caetano, Karlla Antonieta Amorim / Carneiro, Megmar Aparecida Dos Santos / Villar, Livia Melo / Stacciarini, Jeanne-Marie / Martins, Regina Maria Bringel

Viruses

2023 Volume 15, Issue 10

Abstract: A transversal study was conducted among 472 vulnerable individuals (recyclable waste pickers, immigrants and refugees, homeless individuals, as well as lesbian, gay, bisexual, and transexual individuals) in Goiânia City, the capital of the State of Goiás, ...

Abstract	A transversal study was conducted among 472 vulnerable individuals (recyclable waste pickers, immigrants and refugees, homeless individuals, as well as lesbian, gay, bisexual, and transexual individuals) in Goiânia City, the capital of the State of Goiás, Brazil, to investigate the prevalence of hepatitis E virus (HEV) infection. A total of 459 (97.2%) serum samples were tested for anti-HEV IgG and IgM antibodies using fully automated chemiluminescence immunoassays (Liaison
MeSH term(s)	Female ; Humans ; Hepatitis E ; Prevalence ; Brazil/epidemiology ; Seroepidemiologic Studies ; Vulnerable Populations ; Recycling ; Refuse Disposal ; Hepatitis E virus/genetics ; RNA, Viral ; Hepatitis Antibodies ; Immunoglobulin M ; Immunoglobulin G
Chemical Substances	RNA, Viral ; Hepatitis Antibodies ; Immunoglobulin M ; Immunoglobulin G
Language	English
Publishing date	2023-10-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15102070
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Article ; Online: Could Herpesviridae be the cause of severe acute hepatitis of unknown origin in children?

Villar, Livia Melo / da Silva, Lucas Lima / do Lago, Barbara Vieira / Pereira, Jessica Gonçalves / Guimarães, Ana Carolina Silva / Mello, Francisco Campello do Amaral / de Paula, Vanessa Salete

Expert review of anti-infective therapy

2024 Volume 22, Issue 1-3, Page(s) 5–17

Abstract: Introduction: Severe acute hepatitis (SAH) is defined by a severe inflammation of hepatocytes in the liver parenchyma which can lead to an acute liver failure, a clinical condition with high mortality rate that can be triggered by several factors but is ...

Abstract	Introduction: Severe acute hepatitis (SAH) is defined by a severe inflammation of hepatocytes in the liver parenchyma which can lead to an acute liver failure, a clinical condition with high mortality rate that can be triggered by several factors but is usually associated to hepatotropic viruses' infection. In 2022, cases of children with severe acute hepatitis of unknown origin hospitalized in Glasgow, Scotland, were reported. Possible causes of this condition include, but are not limited to, undiagnosed viral (and non-viral) infections, autoimmune hepatitis, drug and/or chemical toxicity, mitochondrial chain respiratory and metabolic disorders. Areas covered: Herpesviruses can cause severe acute hepatitis, but little is known about the role and the mechanisms of herpesviruses as a causative agent of this type of hepatitis. We review the role of herpesviruses as causative agent of SAH in children and other possible mechanisms involved in this disease. Expert opinion: Differential diagnosis for herpesvirus in SAH should be implemented in all settings. Alternative fluids, such as saliva and dried blood, could be used in the diagnosis to overwhelm the availability of biological specimens at sufficient volume. In the future, genetic studies could also be added to increase the knowledge about severe acute hepatitis in children.
MeSH term(s)	Child ; Humans ; Herpesviridae ; Hepatitis ; Virus Diseases ; Diagnosis, Differential ; Acute Disease
Language	English
Publishing date	2024-02-12
Publishing country	England
Document type	Review ; Journal Article
ZDB-ID	2181279-2
ISSN	1744-8336 ; 1478-7210
ISSN (online)	1744-8336
ISSN	1478-7210
DOI	10.1080/14787210.2024.2304637
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Article ; Online: SARS-CoV-2 and dialysis: humoral response, clinical and laboratory impacts before vaccination.

Santos, Alanna Calheiros / Costa, Vanessa Duarte da / Silva, Lucas Lima da / Miguel, Juliana Custódio / Jardim, Rodrigo / Dávila, Alberto Martín Rivera / Paula, Vanessa Salete de / Melgaço, Juliana Gil / Lago, Barbara Vieira do / Villar, Livia Melo

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

2024 Volume 28, Issue 2, Page(s) 103735

Abstract: Background: Patients with kidney disease on Hemodialysis (HD) are susceptible to Coronavirus Disease (COVID-19) due to multiple risk factors.: Aim: This study aims to report the prevalence of antibodies against SARS-CoV-2 among patients on ... ...

Abstract	Background: Patients with kidney disease on Hemodialysis (HD) are susceptible to Coronavirus Disease (COVID-19) due to multiple risk factors. Aim: This study aims to report the prevalence of antibodies against SARS-CoV-2 among patients on hemodialysis before vaccination in Brazil and to compare with clinical, demographic, and laboratory data. Methods: Blood samples from 398 Chronic Kidney Disease (CKD) patients treated in three different private institutions in Rio de Janeiro State, Brazil were submitted to the total anti-SARS-CoV-2 testing. Kidney, liver, and hematological markers were also determined. Respiratory samples were tested by real-time PCR for SARS-CoV-2 RNA and positive samples were subjected to high-throughput sequencing on the MinION device. Results: Overall, anti-SARS-CoV-2 prevalence was 54.5 % (217/398) and two individuals had SARS-CoV-2 RNA with variant B.1.1. High anti-SARS-CoV-2 seroprevalence was found in male gender and those with hospital admission in the last 3-months before the inclusion in the study. Lower red blood cell count was observed in the anti-SARS-CoV-2 seropositive group. High levels of anti-SARS-CoV-2 were found in those who reported symptoms, had low levels of eosinophils and low hematocrit, and who practiced physical activity. Conclusion: High prevalence of anti-SARS-CoV-2 was found in CKD patients before the universal immunization in Brazil suggesting that dialysis patients were highly exposed to SARS-CoV-2.
Language	English
Publishing date	2024-03-08
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2041400-6
ISSN	1678-4391 ; 1413-8670
ISSN (online)	1678-4391
ISSN	1413-8670
DOI	10.1016/j.bjid.2024.103735
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Article ; Online: Avian anti-HBV immunoglobulin: New tool to improve hepatitis B diagnosis methods.

Deodato, Raissa Martins / Dos Santos, Debora Regina Lopes / da Silva, Mayara Torquato Lima / Cruz, Helena Medina / de Paula, Vanessa Salete / Villar, Livia Melo

International journal of biological macromolecules

2023 Volume 253, Issue Pt 3, Page(s) 126644

Abstract: Immunoglobulin Y (IgY) could be used in serological diagnosis focused on several infectious agents. This study aims to produce IgY anti-hepatitis B virus surface antigen (anti-HBs) and to assess its use in enzyme immunoassays. Antibodies were produced by ...

Abstract	Immunoglobulin Y (IgY) could be used in serological diagnosis focused on several infectious agents. This study aims to produce IgY anti-hepatitis B virus surface antigen (anti-HBs) and to assess its use in enzyme immunoassays. Antibodies were produced by immunizing chickens with Hepatitis B vaccine associated (group A), or not, with adjuvant CpG-ODN (group B). Eggs were collected for 20 weeks, yolks were purified based on using polyethylene glycol and affinity chromatography. IgY anti-HBs was featured based on SDS-PAGE and Western Blot techniques. Total protein concentration was measured through spectrophotometry. In-house ELISA used to detect HBsAg was developed based on using IgG/HRP conjugate and IgY-anti-HBs sensitized microplates. Thus, IgY anti-HBs were confirmed through molecular pattern based on SDS-PAGE, whereas specificity of anti-HBs was confirmed through Western Blot. Mean total protein reached 3.27 ± 3.00 mg/mL and 3.11 ± 3.12 mg/mL in groups A and B, respectively. In-house ELISA was developed based on using a panel of HBV positive and negative serum samples; it recorded 100 % sensitivity and 78.9 % specificity to detect HBsAg. In conclusion, it was possible producing anti-HBs IgY by immunizing chickens with HBV vaccine; this molecule could be used as capture antibody to help detecting HBsAg in-house ELISA.
MeSH term(s)	Animals ; Hepatitis B Surface Antigens ; Chickens ; Hepatitis B Vaccines ; Hepatitis B/diagnosis ; Hepatitis B e Antigens ; Hepatitis B Antibodies
Chemical Substances	Hepatitis B Surface Antigens ; Hepatitis B Vaccines ; Hepatitis B e Antigens ; Hepatitis B Antibodies
Language	English
Publishing date	2023-08-31
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.126644
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Article ; Online: High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina.

Lo Castro, Ivana / Espul, Carlos / de Paula, Vanessa Salete / Altabert, Nancy R / Gonzalez, Jorge E / Lago, Barbara Vieira / Villar, Livia Melo

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

2023 Volume 27, Issue 2, Page(s) 102738

Abstract: Environmental surveillance of water sources is important to monitoring viral hepatitis transmission in clinical settings. This study investigated the circulation of hepatitis A (HAV) and E (HEV) viruses in sewage and clinical samples from Argentina. ... ...

Abstract	Environmental surveillance of water sources is important to monitoring viral hepatitis transmission in clinical settings. This study investigated the circulation of hepatitis A (HAV) and E (HEV) viruses in sewage and clinical samples from Argentina. Between 2016 and 2017, 80 raw sewage samples and 86 clinical samples (stool and serum) from suspected cases of hepatitis A and hepatitis E were obtained. HAV and HEV were tested by both real-time and nested PCR. Positive samples were sequenced for genotype determination and phylogenetic analysis. Overall, HAV was recovered in 39% of sewage samples and 61.1% of clinical samples. HEV was detected in 22.5% of sewage samples and 15.9% of clinical samples. HAV was found more frequently in sewage during the winter and in clinical samples in spring; HEV was more prevalent in sewage during summer and in clinical samples in autumn. All HAV isolates belonged to genotype IA and HEV isolates belonged to genotype 3, the most prevalent genotypes in South America. High prevalence of HAV and HEV in environmental and clinical samples in Mendoza, Argentina was observed. These findings reinforce the importance of environmental surveillance and implementation of health strategies to control the spread of HAV and HEV in developing countries.
MeSH term(s)	Humans ; Hepatitis A/epidemiology ; Hepatitis E virus/genetics ; Sewage ; Argentina/epidemiology ; Prevalence ; Phylogeny ; Hepatitis E/epidemiology ; Hepatitis A virus/genetics
Chemical Substances	Sewage
Language	English
Publishing date	2023-01-03
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2041400-6
ISSN	1678-4391 ; 1413-8670
ISSN (online)	1678-4391
ISSN	1413-8670
DOI	10.1016/j.bjid.2022.102738
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Article ; Online: Exploring the potential usefulness of IgY for antiviral therapy: A current review.

da Silva, Mayara Torquato Lima / Deodato, Raissa Martins / Villar, Livia Melo

International journal of biological macromolecules

2021 Volume 189, Page(s) 785–791

Abstract: Immunoglobulin yolk (IgY) is therapeutic antibodies presented in yolk eggs of birds, reptiles, and amphibians. These proteins produced by the immune system of the animal, are capable of neutralizing antigenic molecules, including viral antigens, ... ...

Abstract	Immunoglobulin yolk (IgY) is therapeutic antibodies presented in yolk eggs of birds, reptiles, and amphibians. These proteins produced by the immune system of the animal, are capable of neutralizing antigenic molecules, including viral antigens, fulfilling a role in the body defense. The specificity of these antibodies and the facility for their production, make these molecules capable of being used as tools for diagnosis and immunotherapy. Regarding this last aspect, it is common knowledge that the field of virology, is racing against time in the development of new drugs and vaccines to try to contain pandemics and local epidemics and, in counterproposal, avian antibodies are neutralizing molecules that can help in the control and spread of disease. These molecules have been explored for years and currently chicken eggs are produced in large quantities from the animal's immunization against a specific pathogen. Thus, on this subject, this review made a survey of these researches and presents a summary of all the successful cases and perspectives in the use of IgYs as tools for viral immunization.
MeSH term(s)	Animals ; Antiviral Agents/pharmacology ; Humans ; Immunization ; Immunoglobulins/chemistry ; Immunoglobulins/isolation & purification ; Immunoglobulins/pharmacology ; Virus Diseases/immunology ; Virus Diseases/therapy ; Viruses/drug effects ; Viruses/immunology
Chemical Substances	Antiviral Agents ; IgY ; Immunoglobulins
Language	English
Publishing date	2021-08-17
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2021.08.078
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Article ; Online: Hepatitis E prevalence in indigenous communities from Western Brazilian Amazon.

Villar, Livia Melo / Milagres, Flavio Augusto Pádua / Marques, Julia Trece / de Paula, Vanessa Salete

Liver international : official journal of the International Association for the Study of the Liver

2020 Volume 41, Issue 1, Page(s) 235–236

MeSH term(s)	Brazil/epidemiology ; Hepatitis E/epidemiology ; Hepatitis E virus/genetics ; Humans ; Immunoglobulin G ; Prevalence
Chemical Substances	Immunoglobulin G
Language	English
Publishing date	2020-11-06
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	2102783-3
ISSN	1478-3231 ; 1478-3223
ISSN (online)	1478-3231
ISSN	1478-3223
DOI	10.1111/liv.14679
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