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Article ; Online: Efficacy and safety of chinese herbal foot bath for hypertension: A systematic review and meta-analysis.

Wu, Tong / Zhou, Luolin / Liao, Xinru / He, Yuewen / Xu, Ronglin / Luo, Daihong

2024 Volume 81, Page(s) 103029

Abstract: Background: Hypertension is a prevalent condition with significant impacts on human health. Chinese herbal foot bath, a traditional Chinese medicine treatment, is believed to help manage hypertension.: Objective: This systematic review aims to assess ...

Abstract	Background: Hypertension is a prevalent condition with significant impacts on human health. Chinese herbal foot bath, a traditional Chinese medicine treatment, is believed to help manage hypertension. Objective: This systematic review aims to assess the efficacy of combining Chinese herbal foot bath with conventional treatment for hypertension. Methods: A thorough search across eight databases was conducted to identify relevant studies on Chinese herbal foot bath for hypertension. Randomized controlled trials examining the benefits and risks of using Chinese herbal foot bath combined with conventional treatment for hypertension were included. Blood pressure changes before and after the Chinese herbal foot bath were assessed, and outcomes were analyzed using Review Manager 5.3 software. The certainty of evidence was evaluated using the GRADE tool. Results: Compared to conventional treatment, Chinese herbal foot bath combined with conventional treatment demonstrated greater effectiveness in reducing systolic blood pressure (MD 6.69, 95% CI: 5.86 to 7.53, p < 0.00001) and diastolic blood pressure (MD 5.83, 95% CI: 5.23 to 6.43, p < 0.00001), which reported fewer adverse effects.These studies commonly present issues such as inadequate randomization, lack of blinding, and absence of independent testing for the purity or potency of herbs. Conclusion: The meta-analysis suggests that Chinese herbal foot bath combined with conventional treatment may effectively manage hypertension, with few adverse effects. However, high-quality clinical trials are still needed to confirm these findings due to methodological weaknesses in randomization, blinding, long-term follow-up, and independent testing for the purity and potency of herbs.
MeSH term(s)	Humans ; Drugs, Chinese Herbal/adverse effects ; Medicine, Chinese Traditional ; Phytotherapy ; Hypertension/drug therapy ; Blood Pressure ; Drug-Related Side Effects and Adverse Reactions
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2024-02-23
Publishing country	Scotland
Document type	Meta-Analysis ; Systematic Review ; Journal Article
ZDB-ID	1155895-7
ISSN	1873-6963 ; 0965-2299
ISSN (online)	1873-6963
ISSN	0965-2299
DOI	10.1016/j.ctim.2024.103029
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Article ; Online: Medaka (Oryzias latipes) Olpax6.2 acquires maternal inheritance and germ cells expression, but functionally degenerate in the eye.

Pan, Qihua / Luo, Junzhi / Jiang, Yuewen / Wang, Zhi / Lu, Ke / Chen, Tiansheng

Gene

2023 Volume 872, Page(s) 147439

Abstract: Gene duplication provides raw material for the evolution of genetic and phenotypic complexity. It has remained a long-standing mystery how duplicated genes evolve into new genes by neofunctionalization via the acquisition of new expression and/or ... ...

Abstract	Gene duplication provides raw material for the evolution of genetic and phenotypic complexity. It has remained a long-standing mystery how duplicated genes evolve into new genes by neofunctionalization via the acquisition of new expression and/or activity and simultaneous loss of the old expression and activity. Fishes have many gene duplicates from whole genome duplication, making them excellent for studying the evolution of gene duplicates. In the fish medaka (Oryzias latipes), an ancestral pax6 gene has given rise to Olpax6.1 and Olpax6.2. Here we report that medaka Olpax6.2 is evolving towards neofunctionalization. A chromosomal syntenic analysis indicated that Olpax6.1 and Olpax6.2 are structurally co-homologous to the single pax6 in other organisms. Interestingly, Olpax6.2 maintains all conserved coding exons but loses the non-coding exons of Olpax6.1, and has 4 promoters versus 8 in Olpax6.1. RT-PCR revealed that Olpax6.2 maintains expression in the brain eye, pancreas as Olpax6.1. Surprisingly, Olpax6.2 also exhibits maternal inheritance and gonadal expression by RT-PCR, in situ hybridization and RNA transcriptome analysis. The expression and distribution of Olpax6.2 is not different from Olpax6.1 in the adult brain, eye and pancreas, but exhibited overlapping and distinct expression in early embryogenesis. We show that ovarian Olpax6.2 expression occurs in female germ cells. Olpax6.2 knockout shows no obvious defect in eye development, while Olpax6.1 F0 mutant have severe defects in eye development. Thus, Olpax6.2 acquires maternal inheritance and germ cell expression, but functionally degenerates in the eye, making this gene as an excellent model to study the neofunctionalization of duplicated genes.
MeSH term(s)	Animals ; Female ; Oryzias/genetics ; Oryzias/metabolism ; Maternal Inheritance ; RNA/genetics ; In Situ Hybridization ; Germ Cells
Chemical Substances	RNA (63231-63-0)
Language	English
Publishing date	2023-04-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2023.147439
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Article ; Online: Medaka (Oryzias latipes) Olpax6.2 acquires maternal inheritance and germ cells expression, but functionally degenerate in the eye

Pan, Qihua / Luo, Junzhi / Jiang, Yuewen / Wang, Zhi / Lu, Ke / Chen, Tiansheng

Gene. 2023 July, v. 872 p.147439-

2023

Abstract	Gene duplication provides raw material for the evolution of genetic and phenotypic complexity. It has remained a long-standing mystery how duplicated genes evolve into new genes by neofunctionalization via the acquisition of new expression and/or activity and simultaneous loss of the old expression and activity. Fishes have many gene duplicates from whole genome duplication, making them excellent for studying the evolution of gene duplicates. In the fish medaka (Oryzias latipes), an ancestral pax6 gene has given rise to Olpax6.1 and Olpax6.2. Here we report that medaka Olpax6.2 is evolving towards neofunctionalization. A chromosomal syntenic analysis indicated that Olpax6.1 and Olpax6.2 are structurally co-homologous to the single pax6 in other organisms. Interestingly, Olpax6.2 maintains all conserved coding exons but loses the non-coding exons of Olpax6.1, and has 4 promoters versus 8 in Olpax6.1. RT-PCR revealed that Olpax6.2 maintains expression in the brain eye, pancreas as Olpax6.1. Surprisingly, Olpax6.2 also exhibits maternal inheritance and gonadal expression by RT-PCR, in situ hybridization and RNA transcriptome analysis. The expression and distribution of Olpax6.2 is not different from Olpax6.1 in the adult brain, eye and pancreas, but exhibited overlapping and distinct expression in early embryogenesis. We show that ovarian Olpax6.2 expression occurs in female germ cells. Olpax6.2 knockout shows no obvious defect in eye development, while Olpax6.1 F0 mutant have severe defects in eye development. Thus, Olpax6.2 acquires maternal inheritance and germ cell expression, but functionally degenerates in the eye, making this gene as an excellent model to study the neofunctionalization of duplicated genes.
Keywords	Oryzias latipes ; RNA ; adults ; brain ; embryogenesis ; evolution ; exons ; eyes ; females ; fish ; gene duplication ; germ cells ; gonads ; hybridization ; models ; mutants ; pancreas ; phenotype ; raw materials ; transcriptomics ; Eye ; Genome duplication ; Germ cell ; Pax6 ; PAX6 ; dpf ; PCR ; RT-PCR ; qRT-PCR ; ISH ; PGC ; WGD ; CRISPR/Cas9 ; lnc4 ; Ng ; DAPI ; PD ; HD ; PST ; NBT/BCIP
Language	English
Dates of publication	2023-07
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2023.147439
Database	NAL-Catalogue (AGRICOLA)

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Article: [Analysis of related factors between sudden sensorineural hearing loss and serum indices base on artificial intelligence and big data].

Wang, Zhiwei / Lu, Yuewen / Dou, Xiaohui / Liu, Qianxu / Luo, Bin / Yang, Haidi

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery

2022 Volume 34, Issue 11, Page(s) 977–980

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Artificial Intelligence ; Big Data ; Fibrinogen ; Hearing Loss, Sensorineural ; Hearing Loss, Sudden/diagnosis ; Hearing Loss, Sudden/epidemiology ; Humans
Chemical Substances	Fibrinogen (9001-32-5)
Language	Chinese
Publishing date	2022-03-29
Publishing country	China
Document type	Journal Article
ISSN	2096-7993
ISSN	2096-7993
DOI	10.13201/j.issn.2096-7993.2020.11.004
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Article ; Online: Efficient gene editing in a medaka (

Pan, Qihua / Luo, Junzhi / Jiang, Yuewen / Wang, Zhi / Lu, Ke / Chen, Tiansheng

Journal of Zhejiang University. Science. B

2022 Volume 23, Issue 1, Page(s) 74–83

Abstract: Generation of mutants with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is commonly carried out in fish species by co-injecting a mixture ... ...

Abstract	Generation of mutants with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is commonly carried out in fish species by co-injecting a mixture of
MeSH term(s)	Animals ; CRISPR-Cas Systems ; Cell Line ; Gene Editing ; Oryzias/genetics ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Transfer/genetics
Chemical Substances	RNA, Guide, CRISPR-Cas Systems ; RNA, Transfer (9014-25-9)
Language	English
Publishing date	2022-01-10
Publishing country	China
Document type	Journal Article
ZDB-ID	2247290-3
ISSN	1862-1783 ; 1673-1581
ISSN (online)	1862-1783
ISSN	1673-1581
DOI	10.1631/jzus.B2100343
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Article ; Online: New insights into the stemness of adoptively transferred T cells by γc family cytokines.

Luo, Mengshi / Gong, Wenjian / Zhang, Yuewen / Li, Huayi / Ma, Ding / Wu, Kongming / Gao, Qinglei / Fang, Yong

Cell communication and signaling : CCS

2023 Volume 21, Issue 1, Page(s) 347

Abstract: T cell-based adoptive cell therapy (ACT) has exhibited excellent antitumoral efficacy exemplified by the clinical breakthrough of chimeric antigen receptor therapy (CAR-T) in hematologic malignancies. It relies on the pool of functional T cells to retain ...

Abstract	T cell-based adoptive cell therapy (ACT) has exhibited excellent antitumoral efficacy exemplified by the clinical breakthrough of chimeric antigen receptor therapy (CAR-T) in hematologic malignancies. It relies on the pool of functional T cells to retain the developmental potential to serially kill targeted cells. However, failure in the continuous supply and persistence of functional T cells has been recognized as a critical barrier to sustainable responses. Conferring stemness on infused T cells, yielding stem cell-like memory T cells (T
MeSH term(s)	Cytokines ; CD8-Positive T-Lymphocytes ; Immunotherapy, Adoptive ; Stem Cells ; Signal Transduction
Chemical Substances	Cytokines
Language	English
Publishing date	2023-12-04
Publishing country	England
Document type	Video-Audio Media ; Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2126315-2
ISSN	1478-811X ; 1478-811X
ISSN (online)	1478-811X
ISSN	1478-811X
DOI	10.1186/s12964-023-01354-3
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Article ; Online: Steep-slope vertical-transport transistors built from sub-5 nm Thin van der Waals heterostructures.

Yang, Qiyu / Luo, Zheng-Dong / Duan, Huali / Gan, Xuetao / Zhang, Dawei / Li, Yuewen / Tan, Dongxin / Seidel, Jan / Chen, Wenchao / Liu, Yan / Hao, Yue / Han, Genquan

Nature communications

2024 Volume 15, Issue 1, Page(s) 1138

Abstract: Two-dimensional (2D) semiconductor-based vertical-transport field-effect transistors (VTFETs) - in which the current flows perpendicularly to the substrate surface direction - are in the drive to surmount the stringent downscaling constraints faced by ... ...

Abstract	Two-dimensional (2D) semiconductor-based vertical-transport field-effect transistors (VTFETs) - in which the current flows perpendicularly to the substrate surface direction - are in the drive to surmount the stringent downscaling constraints faced by the conventional planar FETs. However, low-power device operation with a sub-60 mV/dec subthreshold swing (SS) at room temperature along with an ultra-scaled channel length remains challenging for 2D semiconductor-based VTFETs. Here, we report steep-slope VTFETs that combine a gate-controllable van der Waals heterojunction and a metal-filamentary threshold switch (TS), featuring a vertical transport channel thinner than 5 nm and sub-thermionic turn-on characteristics. The integrated TS-VTFETs were realised with efficient current switching behaviours, exhibiting a current modulation ratio exceeding 1 × 10
Language	English
Publishing date	2024-02-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45482-x
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Article ; Online: New insights into the stemness of adoptively transferred T cells by γc family cytokines

Mengshi Luo / Wenjian Gong / Yuewen Zhang / Huayi Li / Ding Ma / Kongming Wu / Qinglei Gao / Yong Fang

Cell Communication and Signaling, Vol 21, Iss 1, Pp 1-

2023 Volume 23

Abstract: Abstract T cell-based adoptive cell therapy (ACT) has exhibited excellent antitumoral efficacy exemplified by the clinical breakthrough of chimeric antigen receptor therapy (CAR-T) in hematologic malignancies. It relies on the pool of functional T cells ... ...

Abstract	Abstract T cell-based adoptive cell therapy (ACT) has exhibited excellent antitumoral efficacy exemplified by the clinical breakthrough of chimeric antigen receptor therapy (CAR-T) in hematologic malignancies. It relies on the pool of functional T cells to retain the developmental potential to serially kill targeted cells. However, failure in the continuous supply and persistence of functional T cells has been recognized as a critical barrier to sustainable responses. Conferring stemness on infused T cells, yielding stem cell-like memory T cells (TSCM) characterized by constant self-renewal and multilineage differentiation similar to pluripotent stem cells, is indeed necessary and promising for enhancing T cell function and sustaining antitumor immunity. Therefore, it is crucial to identify TSCM cell induction regulators and acquire more TSCM cells as resource cells during production and after infusion to improve antitumoral efficacy. Recently, four common cytokine receptor γ chain (γc) family cytokines, encompassing interleukin-2 (IL-2), IL-7, IL-15, and IL-21, have been widely used in the development of long-lived adoptively transferred TSCM in vitro. However, challenges, including their non-specific toxicities and off-target effects, have led to substantial efforts for the development of engineered versions to unleash their full potential in the induction and maintenance of T cell stemness in ACT. In this review, we summarize the roles of the four γc family cytokines in the orchestration of adoptively transferred T cell stemness, introduce their engineered versions that modulate TSCM cell formation and demonstrate the potential of their various combinations. Video Abstract
Keywords	Stemness ; Stem cell-like memory T cells (TSCM) ; Adoptive cell therapy (ACT) ; γc family cytokines ; Medicine ; R ; Cytology ; QH573-671
Subject code	610
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Japanese medaka Olpax6.1 mutant as a potential model for spondylo-ocular syndrome.

Pan, Qihua / Lu, Ke / Luo, Junzhi / Jiang, Yuewen / Xia, Bilin / Chen, Lei / Wang, Mengyang / Dai, Ronggui / Chen, Tiansheng

Functional & integrative genomics

2023 Volume 23, Issue 2, Page(s) 168

Abstract: pax6 is a canonic master gene for eye formation. Knockout of pax6 affects the development of craniofacial skeleton and eye in mice. Whether pax6 affects the development of spinal bone has not been reported yet. In the present study, we used CRISPR/Cas9 ... ...

Abstract	pax6 is a canonic master gene for eye formation. Knockout of pax6 affects the development of craniofacial skeleton and eye in mice. Whether pax6 affects the development of spinal bone has not been reported yet. In the present study, we used CRISPR/Cas9 system to generate Olpax6.1 mutant in Japanese medaka. Phenotype analysis showed that ocular mutation caused by the Olpax6.1 mutation occurred in the homozygous mutant. The phenotype of heterozygotes is not significantly different from that of wild-type. In addition, knockout Olpax6.1 resulted in severe curvature of the spine in the homozygous F2 generation. Comparative transcriptome analysis and qRT-PCR revealed that the defective Olpax6.1 protein caused a decrease in the expression level of sp7, col10a1a, and bglap, while the expression level of xylt2 did not change significantly. The functional enrichment of differentially expressed genes (DEGs) using the Kyoto Encyclopedia of Genes and Genomes database showed that the DEGs between Olpax6.1 mutation and wild-type were enriched in p53 signaling pathway, extracellular matrix (ECM) -receptor interaction, et al. Our results indicated that the defective Olpax6.1 protein results in the reduction of sp7 expression level and the activation of p53 signaling pathway, which leads to a decrease in the expression of genes encoding ECM protein, such as collagen protein family and bone gamma-carboxyglutamate protein, which further inhibits bone development. Based on the phenotype and molecular mechanism of ocular mutation and spinal curvature induced by Olpax6.1 knockout, we believe that the Olpax6.1-/- mutant could be a potential model for the study of spondylo-ocular syndrome.
MeSH term(s)	Animals ; Mice ; Oryzias/genetics ; Oryzias/metabolism ; Mice, Knockout ; Tumor Suppressor Protein p53/genetics ; Mutation
Chemical Substances	Tumor Suppressor Protein p53
Language	English
Publishing date	2023-05-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2014670-X
ISSN	1438-7948 ; 1438-793X
ISSN (online)	1438-7948
ISSN	1438-793X
DOI	10.1007/s10142-023-01090-4
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Article ; Online: Quanzhen Yiqi decoction attenuates inflammation in mice with smoking-induced COPD by activating the Nrf2/HO-1 pathway and inhibiting the NLRP3 inflammasome.

Zeng, Jiamin / Li, Zhenqiu / Li, Wanyan / Liang, Zhu / Luo, Yuewen / Li, Caiping / Liao, Sida / Wang, Kexin / Hu, Yuanlong / Li, Yuanyuan / Liang, Qiuling / Lu, Wenju / Li, Lu / Wu, Zhijuan / Zhang, Dapeng / Zhang, Zhimin

The Journal of pharmacy and pharmacology

2024

Abstract: Objective: Quanzhen Yiqi decoction (QZYQ) is a traditional Chinese medicine for treating chronic obstructive pulmonary disease.: Methods: Mice were exposed to cigarette smoke (CS) 6 days/week (40 cigarettes/day) for 24 weeks and then intragastrically ...

Abstract	Objective: Quanzhen Yiqi decoction (QZYQ) is a traditional Chinese medicine for treating chronic obstructive pulmonary disease. Methods: Mice were exposed to cigarette smoke (CS) 6 days/week (40 cigarettes/day) for 24 weeks and then intragastrically administered QZYQ (4.72, 9.45, or 18.89 g/kg) or dexamethasone (DEX, 0.6 mg/kg) for 6 weeks. We examined the lung function and collected bronchoalveolar lavage fluid for inflammatory cell and cytokine quantification. The pathological lung changes, ROS and oxidative biomarkers were measured. We used immunohistochemistry and western blotting to evaluate the levels of Nrf2/HO-1, NLRP3/ASC/Caspase1/IL-1β/IL-18. Results: The CS group showed significant increases in the forced vital capacity, lung resistance, and chord compliance and a lower FEV50/FVC compared with the control, and QZYQ improved these changes. In addition, QZYQ effectively reduced emphysema, immune cell infiltration, and airway remodeling. QZYQ stimulated HO-1 expression and reduced oxidative stress through the Nrf2 pathway. QZYQ inhibited the production of NLRP3/ASC/Caspase-1 to inhibit IL-1β and IL-18. Conclusion: Our study suggested that QZYQ can improve the function and histology of the lungs and reduce inflammatory cell recruitment. QZYQ inhibits ROS production and NLRP3 inflammasome activation by upregulating Nrf2 to reduce lung injury. The anti-inflammatory effects of QZYQ are similar to those of DEX.
Language	English
Publishing date	2024-04-26
Publishing country	England
Document type	Journal Article
ZDB-ID	3107-0
ISSN	2042-7158 ; 0022-3573 ; 0373-1022
ISSN (online)	2042-7158
ISSN	0022-3573 ; 0373-1022
DOI	10.1093/jpp/rgae047
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