Article ; Online: Using 2D-IR Spectroscopy to Measure the Structure, Dynamics, and Intermolecular Interactions of Proteins in H
2024 Volume 57, Issue 5, Page(s) 685–692
Abstract: ConspectusInfrared (IR) spectroscopy probes molecular structure at the level of the chemical bond or functional group. In the case of proteins, the most informative band in the IR spectrum is the amide I band, which arises predominantly from the C═O ... ...
| Abstract | ConspectusInfrared (IR) spectroscopy probes molecular structure at the level of the chemical bond or functional group. In the case of proteins, the most informative band in the IR spectrum is the amide I band, which arises predominantly from the C═O stretching vibration of the peptide link. The folding of proteins into secondary and tertiary structures leads to vibrational coupling between peptide units, generating specific amide I spectral signatures that provide a fingerprint of the macromolecular conformation. Ultrafast two-dimensional IR (2D-IR) spectroscopy allows the amide I band of a protein to be spread over a second frequency dimension in a way that mirrors 2D-NMR methods. This means that amide I 2D-IR spectroscopy produces a spectral map that is exquisitely sensitive to protein structure and dynamics and so provides detailed insights that cannot be matched by IR absorption spectroscopy. As a result, 2D-IR spectroscopy has emerged as a powerful tool for probing protein structure and dynamics over a broad range of time and length scales in the solution phase at room temperature. However, the protein amide I band coincides with an IR absorption from the bending vibration of water (δ |
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| MeSH term(s) | Spectrophotometry, Infrared/methods ; Proteins/chemistry ; Peptides ; Amides/chemistry ; Vibration ; Solvents |
| Chemical Substances | Proteins ; Peptides ; Amides ; Solvents |
| Language | English |
| Publishing date | 2024-02-16 |
| Publishing country | United States |
| Document type | Review ; Journal Article |
| ZDB-ID | 1483291-4 |
| ISSN | 1520-4898 ; 0001-4842 |
| ISSN (online) | 1520-4898 |
| ISSN | 0001-4842 |
| DOI | 10.1021/acs.accounts.3c00682 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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