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Article: Validation of the Anticolitis Efficacy of the Jian-Wei-Yu-Yang Formula.

Yan, Jing / Tang, Yan / Yu, Wei / Jiang, Lu / Liu, Chen / Li, Qi / Zhang, Zhiqiang / Shao, Changlei / Zheng, Yang / Liu, Xihao / Liu, Xincheng

Evidence-based complementary and alternative medicine : eCAM

2022 Volume 2022, Page(s) 9110704

Abstract: ... to its repetitive remission and relapse. The Jian-Wei-Yu-Yang (JW) formula has a historical application ...

Abstract	Background: Inflammatory bowel disease (IBD) is a major cause of morbidity and mortality due to its repetitive remission and relapse. The Jian-Wei-Yu-Yang (JW) formula has a historical application in the clinic to combat gastrointestinal disorders. The investigation aimed to explore the molecular and cellular mechanisms of JW. Methods: 2% dextran sodium sulfate (DSS) was diluted in drinking water and given to mice for 5 days to establish murine models of experimental colitis, and different doses of JW solution were administered for 14 days. Network pharmacology analysis and weighted gene co-expression network analysis (WGCNA) were utilized to predict the therapeutic role of JW against experimental colitis and colitis-associated colorectal cancer (CAC). 16S rRNA sequencing and untargeted metabolomics were conducted using murine feces. Western blotting, immunocytochemistry, and wound healing experiments were performed to confirm the molecular mechanisms. Results: (1) Liquid chromatography with mass spectrometry was utilized to confirm the validity of the JW formula. The high dose of JW treatment markedly attenuated DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis. (2) The JW targets were related to the survival probability in patients with colorectal cancer, underlying a potential therapeutic value in CRC intervention. (3) Moreover, the JW therapy successfully rescued the decreased richness and diversity of microbiota, suppressed the potentially pathogenic phenotype of the gut microorganisms, and increased cytochrome P450 activity in murine colitis models. (4) Our Conclusion: The JW capsule attenuated the progression of murine colitis by a prompt resolution of inflammation and bloody stool and by re-establishing a microbiome profile that favors re-epithelization and prevents carcinogenesis.
Language	English
Publishing date	2022-08-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2022/9110704
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Article: The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation.

Yu, Wei / Li, Qi / Shao, Changlei / Zhang, Yijia / Kang, Cai / Zheng, Yang / Liu, Xihao / Liu, Xincheng / Yan, Jing

Frontiers in pharmacology

2022 Volume 13, Page(s) 946065

Abstract: ... Xiang-Wei-Kang (CW) formula has been utilized to treat gastrointestinal disorders in the clinic ...

Abstract	Inflammatory bowel disease (IBD) is pathologically characterized by an immune response accommodative insufficiency and dysbiosis accompanied by persistent epithelial barrier dysfunction. The Cao-Xiang-Wei-Kang (CW) formula has been utilized to treat gastrointestinal disorders in the clinic. The present study was designed to delineate the pharmacological mechanisms of this formula from different aspects of the etiology of ulcerative colitis (UC), a major subtype of IBD. Dextran sodium sulfate (DSS) was given to mice for a week at a concentration of 2%, and the CW solution was administered for 3 weeks. 16S rRNA gene sequencing and untargeted metabolomics were conducted to examine the changes in the microbiome profile, and biochemical experiments were performed to confirm the therapeutic functions predicted by system pharmacology analysis. The CW treatment hampered DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis, which was corroborated by suppressed caspase 3 (Casp3) and interleukin-1b (IL-1b) and increased cleaved caspase 3 expression and casp-3 activity in the colon samples from colitis mice subjected to the CW therapy. Moreover, the CW therapy rescued the decreased richness and diversity, suppressed the potentially pathogenic phenotype of the gut microorganisms, and reversed the altered linoleic acid metabolism and cytochrome P450 activity in murine colitis models. In our
Language	English
Publishing date	2022-09-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.946065
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Article ; Online: Tibetan medicine Si-Wei-Qiang-Wei Powder ameliorates cholecystitis via inhibiting the production of pro-inflammatory cytokines and regulating the MAPK signaling pathway.

Zheng, Zhe / Xiong, Hui / Zhao, Zhongqiu / Zhou, Keli / Fu, Miao / Liu, Xinqiao / Mei, Zhinan

Journal of ethnopharmacology

2022 Volume 303, Page(s) 116026

Abstract: Ethnopharmacological relevance: Si-Wei-Qiang-Wei Powder (SWQ) is a formulated ...

Abstract	Ethnopharmacological relevance: Si-Wei-Qiang-Wei Powder (SWQ) is a formulated traditional Tibetan medicine preparation that has been used clinically to treat liver and gallbladder diseases for centuries. Previous work has confirmed its clinical effectiveness, however, the specific mechanism of SWQ is still unknown. Aim of the study: This study aims to explore the anti-inflammatory effect of SWQ on cholecystitis and its possible mechanism. Materials and methods: The main chemical components of SWQ were analyzed by HPLC. The network pharmacology database was used to screen and construct the network of the main components and molecular targets of SWQ, and to predict the molecular pathways of its core targets. Cholecystitis guinea pig model and LPS stimulated cultured human gallbladder epithelial cells (HGBEC) were used, as in vivo and in vitro methods respectively, to study the anti-cholecystitis activity of SWQ. Specifically, gallbladder wall thickness, hematoxylin-eosin (H&E) staining, and liver function indexes were used to evaluate the anti-inflammatory activities of SWQ in cholecystitis; qRT-PCR and ELISA were used to detect the changes of the production of inflammatory cytokines; Western blot analysis was used to analyze the effects of SWQ on phosphorylation of P38, ERK1/2, JNK and AKT. Results: SWQ decreased the indexes of ALT, AST, TBA, CHOL, DBIL in serum and TBIL, TC and Ca Conclusion: In summary, our research showed that SWQ relieves gallbladder inflammation by inhibiting the MAPK pathway.
Language	English
Publishing date	2022-12-09
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.116026
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Article ; Online: Integration of gut microbiome and serum metabolome revealed the effect of Qing-Wei-Zhi-Tong Micro-pills on gastric ulcer in rats.

Wang, Chao / Jiang, Shengyu / Zheng, Haoyu / An, Yiming / Zheng, Wenxue / Zhang, Jiaqi / Liu, Jianming / Lin, Hongqiang / Wang, Guoqiang / Wang, Fang

Journal of ethnopharmacology

2023 Volume 319, Issue Pt 3, Page(s) 117294

Abstract: Ethnopharmacological relevance: Qing-Wei-Zhi-Tong Micro-pills (QWZT) is herbal compound used ...

Abstract	Ethnopharmacological relevance: Qing-Wei-Zhi-Tong Micro-pills (QWZT) is herbal compound used in the treatment of GU, whose functions include clearing the stomach and fire, softening the liver and relieving pain. However, its mechanistic profile on host intestinal microbiota and metabolism has not been determined. Aim of the study: The present study aimed to observe the healing effect of QWZT on acetic acid-induced gastric ulcer in a rat model and to preliminarily elucidate its possible therapeutic mechanism from the perspective of host intestinal microbiota and metabolism. Materials and methods: The Wistar male rats (7 weeks old; weight 180-200 g) were randomly divided into normal control group (NC), acetic acid-induced gastric ulcer group (GU), and QWZT treatment group (High dose: 1250 mg/kg/day, Middle dose: 625 mg/kg/day, Low dose: 312.5 mg/kg/day) of 6 rats each. An acetic acid-induced gastric ulcer rat model was constructed based on anatomical surgery. QWZT (High dose, Middle dose, and Low dose) was used to treat gastric ulcer rats for 7 days by gavage. At the end of treatment, the body weight, macroscopic condition of gastric tissue ulcers, pathological changes (HE staining), inflammatory factors, oxidative stress factors, and endocrine factors were assessed in each group of rats. Fresh feces and serum from each group of rats were collected for microbiome and metabolome analysis on the machine, respectively. Drug-disease common targets and functional pathways were captured based on network pharmacology. The complex network of Herbs-Targets-Pathways-Metabolites-Microbiota interactions was constructed. Ultimately, Fecal Microbiota Transplantation (FMT) evaluated the contribution of gut microbiota in disease. Results: QWZT increased the abundance of beneficial bacteria (Bacteroides, Alloprevotella, Rikenellaceae_RC9_gut_group, Lactobacillus, Lachnospiraceae_NK4A136_group, Parabacteroides, etc.), reduced the abundance of harmful bacteria (Micromonospora, Geobacter, Nocardioides, and Arenimonas, etc.), reduced the levels of inflammatory mediators (12,13-EpOME, 9,10-Epoxyoctadecenoic acid, SM(d18:1/16:0) and Leukotriene A4, etc.), restored host metabolic disorders (Linoleic acid metabolism, Glycerophospholipid metabolism, and Arachidonic acid metabolism), and regulated the level of cytokines (IL-6, TNF-a, SOD, MDA, PEG-2 and NO), ultimately exerting an anti-ulcer effect. Apart from that, FMT improved acetic acid-induced gastric ulcers in rats. Conclusion: QWZT improved acetic acid-induced gastric ulcers in rats by remodeling intestinal microbiota and regulating host metabolism. This work may promote the process of developing and utilizing clinical applications of QWZT.
MeSH term(s)	Male ; Rats ; Animals ; Stomach Ulcer/chemically induced ; Stomach Ulcer/drug therapy ; Gastrointestinal Microbiome ; Rats, Wistar ; Metabolome ; Acetic Acid
Chemical Substances	Acetic Acid (Q40Q9N063P)
Language	English
Publishing date	2023-10-14
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117294
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Article: Tibetan Medicine Shi-Wei-Gan-Ning-San Alleviates Carbon Tetrachloride-Induced Chronic Liver Injury by Inhibiting TGF-

Jia, Ziming / Zheng, Yanhua / Fu, Shaohua / Qu, Jingjing / Tian, Jie / Qu, Wen / Mei, Zhinan

Evidence-based complementary and alternative medicine : eCAM

2022 Volume 2022, Page(s) 2011876

Abstract: Background: Shi-Wei-Gan-Ning-San (SWGNS) is a classic Tibetan prescription, which has obvious ...

Abstract	Background: Shi-Wei-Gan-Ning-San (SWGNS) is a classic Tibetan prescription, which has obvious clinical effects in the treatment of viral hepatitis, fatty liver, liver fibrosis, liver cirrhosis, liver cancer, and other liver injuries. However, animal studies and mechanism studies are still lacking. This study aimed to investigate its hepatoprotective efficacy and pharmacological mechanism in animal experiments. Methods: Chronic liver injury was induced by oral administration of carbon tetrachloride (CCl Results: SWGNS reversed weight loss is induced by CCl Conclusions: In conclusion, SWGNS alleviated CCl
Language	English
Publishing date	2022-08-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2022/2011876
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Zs.A 5995: Show issues

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Article: A research on the mechanism of NSAID-related gastric ulcer treated by jia wei wu qi san based on the p38mapk signal pathway.

Zheng, Liang / Wang, Pei / Wang, Yuan-Yuan / Li, Zhen / Tian, Ye

Pakistan journal of pharmaceutical sciences

2021 Volume 34, Issue 2, Page(s) 585–589

Abstract: This study aims to explore the mechanism of NSAID-related gastric ulcer treated by JIA WEI WU QI ... was given rabeprazole (2mg/kg), and group D was administered with JIA WEI WU QI SAN (2g/kg ... D (P<0.05). (4) The expression of p-P38MAPK decreased in group D (P<0.05). JIA WEI WU QI SAN has ...

Abstract	This study aims to explore the mechanism of NSAID-related gastric ulcer treated by JIA WEI WU QI SAN. Clean-grade SD rats were randomly divided into four groups. Group A was assigned as the control group. Groups B, C and D were intragastrically administered with 2.5mg/kg of indomethacin solution QD after 48 hours. After 15 days of treatment, group B was administered with 0.9% sodium chloride, group C was given rabeprazole (2mg/kg), and group D was administered with JIA WEI WU QI SAN (2g/kg). Abdominal aorta sampling was performed, and gastric tissues were isolated on the 29th day. The protein expression of p-P38MAPK and COX-2 were detected by western blot, while the concentration of PGE2 and IL-1 were determined by ELISA. (1) The expression of IL-1ingroup B dramatically declined in group D (P<0.01). (2)The expression of PGE-2dramatically increased in group D(P<0.01). (3) The expression of COX-2 increased in group D (P<0.05). (4) The expression of p-P38MAPK decreased in group D (P<0.05). JIA WEI WU QI SAN has multiple functions, including the activation of the p-P38MAPK signaling pathway, which promote the activation of COX-2, induce the arachidonic acid to increase the level of PG, and decrease the concentration of IL-1, thereby inducing an inflammatory reaction, and promote gastric mucosa repair.
MeSH term(s)	Animals ; Anti-Inflammatory Agents, Non-Steroidal/adverse effects ; Arachidonic Acid/metabolism ; Cyclooxygenase 2/drug effects ; Cyclooxygenase 2/metabolism ; Dinoprostone/metabolism ; Drugs, Chinese Herbal/pharmacology ; Indomethacin/adverse effects ; Interleukin-1/metabolism ; Rats ; Rats, Sprague-Dawley ; Signal Transduction/drug effects ; Stomach/drug effects ; Stomach/metabolism ; Stomach Ulcer/chemically induced ; Stomach Ulcer/metabolism ; p38 Mitogen-Activated Protein Kinases/drug effects ; p38 Mitogen-Activated Protein Kinases/metabolism
Chemical Substances	Anti-Inflammatory Agents, Non-Steroidal ; Drugs, Chinese Herbal ; Interleukin-1 ; Arachidonic Acid (27YG812J1I) ; Cyclooxygenase 2 (EC 1.14.99.1) ; p38 Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; Dinoprostone (K7Q1JQR04M) ; Indomethacin (XXE1CET956)
Language	English
Publishing date	2021-07-19
Publishing country	Pakistan
Document type	Journal Article
ZDB-ID	885131-1
ISSN	1011-601X
ISSN	1011-601X
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Zs.A 6588: Show issues

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Article ; Online: Retraction for Wei et al., "The Breast Cancer Susceptibility Gene Product Fibroblast Growth Factor Receptor 2 Serves as a Scaffold for Regulation of NF-κB Signaling".

Wei, Wangzhi / Liu, Wei / Cassol, Clarissa A / Zheng, Weiyue / Asa, Sylvia L / Ezzat, Shereen

Molecular and cellular biology

2018 Volume 38, Issue 16

Language	English
Publishing date	2018-07-30
Publishing country	United States
Document type	Journal Article ; Retraction of Publication
ZDB-ID	779397-2
ISSN	1098-5549 ; 0270-7306
ISSN (online)	1098-5549
ISSN	0270-7306
DOI	10.1128/MCB.00298-18
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Zs.A 1666: Show issues

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Article: Delayed morphological adjustment of the Yellow and lower Wei Rivers upstream of the Sanmenxia dam at multi-temporal scales

Zheng, Shan / Wang, Hualin / Wu, Baosheng

Catena. 2022 May, v. 212

2022

Abstract: ... at the middle Yellow and lower Wei Rivers upstream of the Sanmenxia Dam, China, based on data of water volume ... The retrogressive aggradation migrated ∼200 km upstream to the tributary of the Yellow River, i.e., the Wei River ...

Abstract	The delayed response of channel evolution upstream of dams to base-level changes has not yet been thoroughly understood. In this paper, we studied the delayed response of the morphological adjustment at the middle Yellow and lower Wei Rivers upstream of the Sanmenxia Dam, China, based on data of water volume, sediment load, cross-sectional profiles, and erosion/deposition volumes during 1960–2018. Channel evolution upstream of the dam obviously lagged behind the dynamic pool level fluctuation during the first approximately two decades following dam closure, when retrogressive aggradation/erosion phases were identified. The retrogressive aggradation migrated ∼200 km upstream to the tributary of the Yellow River, i.e., the Wei River. The impact of base-level rise may propagate backwards behind the beginning of the backwater reach, reflecting indirect backwater effects. The dam has been operated by the controlled release of “storing the clear water during nonflood seasons and discharging the turbid water during flood seasons” since 1974, and the reservoir has generally been eroded during flood seasons and aggraded during nonflood seasons. The erosion center was defined as the subreach between two adjacent cross-sections with the greatest erosion rate during the flood season, and the aggradation center was defined as that with the greatest aggradation rate during the nonflood season. The results showed that the erosion/deposition centers migrated towards the dam by ∼50–60 km during 1974–2010 and migrated upstream after ∼2010. The location of the erosion/deposition centers was related not only to the water and sediment conditions and backwater length in the current year but also to these conditions in the previous ∼4 years, reflecting the delayed response of channel adjustment or the cumulative impact of pervious conditions on channel morphology. This study helps to inform future reservoir management and the delayed channel response to base level change caused by different natural and anthropogenic disturbances.
Keywords	catenas ; evolution ; rivers ; sediment contamination ; sediments ; China ; Yellow River
Language	English
Dates of publication	2022-05
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	519608-5
ISSN	1872-6887 ; 0008-7769 ; 0341-8162
ISSN (online)	1872-6887 ; 0008-7769
ISSN	0341-8162
DOI	10.1016/j.catena.2022.106035
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Tibetan Medicine Shi-Wei-Gan-Ning-San Alleviates Carbon Tetrachloride-Induced Chronic Liver Injury by Inhibiting TGF-β1 in Wistar Rats

Ziming Jia / Yanhua Zheng / Shaohua Fu / Jingjing Qu / Jie Tian / Wen Qu / Zhinan Mei

Evidence-Based Complementary and Alternative Medicine, Vol

2022 Volume 2022

Abstract: Background. Shi-Wei-Gan-Ning-San (SWGNS) is a classic Tibetan prescription, which has obvious ...

Abstract	Background. Shi-Wei-Gan-Ning-San (SWGNS) is a classic Tibetan prescription, which has obvious clinical effects in the treatment of viral hepatitis, fatty liver, liver fibrosis, liver cirrhosis, liver cancer, and other liver injuries. However, animal studies and mechanism studies are still lacking. This study aimed to investigate its hepatoprotective efficacy and pharmacological mechanism in animal experiments. Methods. Chronic liver injury was induced by oral administration of carbon tetrachloride (CCl4) in Wistar rats for 13 weeks. SWGNS was administered orally to rats at doses of 235, 705, and 1410 mg/kg for 13 weeks. Blood samples were collected for biochemical, ELISA, and radioimmunoassay. Livers were harvested for H&E and immunohistochemical staining. The major constituents of SWGNS were analyzed by HPLC. In vitro experiments were used to explore the protective effect of Crocin on BRL-3A in the environment of H2O2. Results. SWGNS reversed weight loss is induced by CCl4. Serum assays showed that SWGNS reduced CCl4-induced alanine aminotransferase, aspartate aminotransferase, total bilirubin, and γ-glutamyltransferase levels and increased the total protein and albumin levels. Histopathological evaluation showed that SWGNS alleviated hepatic steatosis, fibrosis, and inflammation. Furthermore, SWNGS reduced CCl4-induced elevations of TGF-β1, hyaluronic acid, laminin, and collagen IV in serum and reduced the high expression of α-SMA in tissues. Moreover, Crocin I and II are the main components of SWGNS. Crocin attenuated the damaging effects of H2O2 on BRL-3A. Conclusions. In conclusion, SWGNS alleviated CCl4-induced chronic liver injury by inhibiting the TGF-β1 pathway. This plays an important role in promoting traditional Tibetan medicine in clinical practice.
Keywords	Other systems of medicine ; RZ201-999
Subject code	610
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A novel comprehensive model of set pair analysis with extenics for river health evaluation and prediction of semi-arid basin - A case study of Wei River Basin, China.

Wan, Xuhao / Yang, Tao / Zhang, Qian / Yan, Xuerong / Hu, Changtong / Sun, Laikang / Zheng, Yiwen

The Science of the total environment

2021 Volume 775, Page(s) 145845

Abstract: ... sustainable development, this study is that health evaluation index system is established in the Wei River Basin, covering ... with extenics is established to assess and predict health. The results show that Wei River System and Jing ... River System are healthy, Beiluo River System is sub-healthy, and the whole Wei River Basin is also healthy ...

Abstract	The accelerated development of urbanization in semi-arid areas is easy to cause varying degrees of disturbance to its fragile aquatic ecosystem. To find a general method for assessing the health status in semi-arid basins in China, and to provide theoretical basis for river health management and sustainable development, this study is that health evaluation index system is established in the Wei River Basin, covering a huge semi-arid area, and analyzed the biological structure of plankton obtained by field sampling and identified in laboratory, chemical conditions including dissolved oxygen, water temperature, ammonia nitrogen, pH, chlorophyll and other water physical and chemical factors determined by field instruments and sediment heavy metals analyzed in laboratory, physical habitat scored on site and social factors including water resource utilization rate and water consumption per 10,000 yuan of GDP collected in local water resource bulletin. Based on the idea of game theory, an improved coupling model of set pair analysis with extenics is established to assess and predict health. The results show that Wei River System and Jing River System are healthy, Beiluo River System is sub-healthy, and the whole Wei River Basin is also healthy; most of the cross-sections have a tendency to change to a bad level. Compared with simple weighting, set pair analysis and variable fuzzy set methods based on the corresponding sampling data and the weight in this paper, the coupling model can explain the transition and its trend between levels, reflect the certainty and uncertainty, and get more accurate results. It is suggested that daily monitoring and management should be strengthened in most sections to improve their health. And improve the development level of ecological function and social service function, give priority to the development of downstream water resources economy.
Language	English
Publishing date	2021-02-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2021.145845
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