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Article ; Online: NEM MORCEGOS, NEM PANGOLIM

Lucio Ayres Caldas

Continentes, Iss

FOI O RINOCERONTE

2020 Volume 16

Abstract: Resumo: O presente texto apresenta elementos no campo biológico que caracterizam o coronaviruz, destaca que a ampliação desta e de outras doenças ocorrem por um esgotamento ecológico e que a proporção de impacto do coronavírus, no contexto econômico ... ...

Abstract	Resumo: O presente texto apresenta elementos no campo biológico que caracterizam o coronaviruz, destaca que a ampliação desta e de outras doenças ocorrem por um esgotamento ecológico e que a proporção de impacto do coronavírus, no contexto econômico capitalista, possui dimensões catastróficas que invadem dimensões seníveis da vida contida entre o cotidiano, o trabalho e a produção de valor. Palavras Chaves: Coronavirus, origem, impacto societário. NEITHER BATS NOR PANGOLIN: IT WAS THE RHINOCEROS Abstract: This text presents elements in the biological field that characterize the coronaviruz, highlights that the expansion of this and other diseases occur by an ecological exhaustion and that the proportion of coronavirus impact, in the capitalist economic context, has catastrophic dimensions that invade sensitive dimensions of life contained between daily life, work and value production. Keywords: Coronavirus, origin, social impact. NI MURCIÉLAGOS NI PANGOLÍN: ERA EL RINOCERONTE Resumen: Este texto presenta elementos en el campo biológico que caracterizan al coronaviruz, destaca que la expansión de esta y otras enfermedades se producen por un agotamiento ecológico y que la proporción de impacto del coronavirus, en el contexto económico capitalista, tiene dimensiones catastróficas que invaden dimensiones sensibles de la vida contenidas entre la vida cotidiana, el trabajo y la producción de valor. Palabras clave: Coronavirus, origen, impacto social
Keywords	Coronavirus ; origem ; impacto social ; Physical geography ; GB3-5030 ; Geography (General) ; G1-922
Language	Portuguese
Publishing date	2020-06-01T00:00:00Z
Publisher	Universidade Federal Rural do Rio de Janeiro
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: A Window to

Caldas, Lucio Ayres / de Souza, Wanderley

Pathogens (Basel, Switzerland)

2018 Volume 7, Issue 3

Abstract: ... ...

Abstract	The
Language	English
Publishing date	2018-08-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens7030069
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Article ; Online: SARS-CoV-2 egress from Vero cells: a morphological approach.

Caldas, Lucio Ayres / Carneiro, Fabiana Avila / Augusto, Ingrid / Corrêa, Isadora Alonso / da Costa, Luciana Jesus / Miranda, Kildare / Tanuri, Amilcar / de Souza, Wanderley

Histochemistry and cell biology

2023 Volume 161, Issue 1, Page(s) 59–67

Abstract: Despite being extensively studied because of the current coronavirus disease 2019 (COVID-19) pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interactions with mammalian cells are still poorly understood. Furthermore, little is ... ...

Abstract	Despite being extensively studied because of the current coronavirus disease 2019 (COVID-19) pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interactions with mammalian cells are still poorly understood. Furthermore, little is known about this coronavirus cycle within the host cells, particularly the steps that lead to viral egress. This study aimed to shed light on the morphological features of SARS-CoV-2 egress by utilizing transmission and high-resolution scanning electron microscopy, along with serial electron tomography, to describe the route of nascent virions towards the extracellular medium. Electron microscopy revealed that the clusters of viruses in the paracellular space did not seem to result from collective virus release. Instead, virus accumulation was observed on incurved areas of the cell surface, with egress primarily occurring through individual vesicles. Additionally, our findings showed that the emission of long membrane projections, which could facilitate virus surfing in Vero cells infected with SARS-CoV-2, was also observed in non-infected cultures, suggesting that these are constitutive events in this cell lineage.
MeSH term(s)	Animals ; Chlorocebus aethiops ; Vero Cells ; SARS-CoV-2 ; Cell Line ; COVID-19 ; Microscopy, Electron, Scanning ; Mammals
Language	English
Publishing date	2023-09-22
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1222930-1
ISSN	1432-119X ; 0301-5564 ; 0948-6143
ISSN (online)	1432-119X
ISSN	0301-5564 ; 0948-6143
DOI	10.1007/s00418-023-02239-9
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Article ; Online: Cost-effective 3D lung tissue spheroid as a model for SARS-CoV-2 infection and drug screening.

Miranda, Guilherme A S C / Corrêa, Isadora Alonso / Amorim, Érica Almeida / Caldas, Lucio Ayres / Carneiro, Fabiana Ávila / da Costa, Luciana Jesus / Granjeiro, José Mauro / Tanuri, Amilcar / de Souza, Wanderley / Baptista, Leandra Santos

Artificial organs

2024

Abstract: Background: The SARS-CoV-2 pandemic has spurred an unparalleled scientific endeavor to elucidate the virus' structure, infection mechanisms, and pathogenesis. Two-dimensional culture systems have been instrumental in shedding light on numerous aspects ... ...

Abstract	Background: The SARS-CoV-2 pandemic has spurred an unparalleled scientific endeavor to elucidate the virus' structure, infection mechanisms, and pathogenesis. Two-dimensional culture systems have been instrumental in shedding light on numerous aspects of COVID-19. However, these in vitro systems lack the physiological complexity to comprehend the infection process and explore treatment options. Three-dimensional (3D) models have been proposed to fill the gap between 2D cultures and in vivo studies. Specifically, spheroids, composed of lung cell types, have been suggested for studying SARS-CoV-2 infection and serving as a drug screening platform. Methods: 3D lung spheroids were prepared by coculturing human alveolar or bronchial epithelial cells with human lung stromal cells. The morphology, size, and ultrastructure of spheroids before and after SARS-CoV-2 infection were analyzed using optical and electron microscopy. Immunohistochemistry was used to detect spike protein and, thus, the virus presence in the spheroids. Multiplex analysis elucidated the cytokine release after virus infection. Results: The spheroids were stable and kept their size and morphology after SARS-CoV-2 infection despite the presence of multivesicular bodies, endoplasmic reticulum rearrangement, tubular compartment-enclosed vesicles, and the accumulation of viral particles. The spheroid responded to the infection releasing IL-6 and IL-8 cytokines. Conclusion: This study demonstrates that coculture spheroids of epithelial and stromal cells can serve as a cost-effective infection model for the SARS-CoV-2 virus. We suggest using this 3D spheroid as a drug screening platform to explore new treatments related to the cytokines released during virus infection, especially for long COVID treatment.
Language	English
Publishing date	2024-02-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	441812-8
ISSN	1525-1594 ; 0160-564X
ISSN (online)	1525-1594
ISSN	0160-564X
DOI	10.1111/aor.14729
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Article ; Online: A Window to Toxoplasma gondii Egress

Lucio Ayres Caldas / Wanderley de Souza

Pathogens, Vol 7, Iss 3, p

2018 Volume 69

Abstract: The Toxoplasma gondii cellular cycle has been widely studied in many lifecycle stages; however, the egress event still is poorly understood even though different types of molecules were shown to be involved. Assuming that there is no purpose or ... ...

Abstract	The Toxoplasma gondii cellular cycle has been widely studied in many lifecycle stages; however, the egress event still is poorly understood even though different types of molecules were shown to be involved. Assuming that there is no purpose or intentionality in biological phenomena, there is no such question as “Why does the parasite leaves the host cell”, but “Under what conditions and how?”. In this review we aimed to summarize current knowledge concerning T. gondii egress physiology (signalling pathways), structures, and route.
Keywords	toxoplasma gondii ; toxoplasma egress ; electron microscopy ; Medicine ; R
Language	English
Publishing date	2018-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model.

Caldas, Lucio Ayres / Ferreira, Davis Fernandes / Freitas, Tânia Rosaria Pereira

Revista da Sociedade Brasileira de Medicina Tropical

2018 Volume 51, Issue 5, Page(s) 584–590

Abstract: Introduction: The Mayaro virus (MAYV), which is an arbovirus closely related to the Chikungunya virus, causes a dengue-like acute illness that is endemic to Central and South America. We investigated the anti-MAYV activity of prostaglandin A1 (PGA1), a ... ...

Abstract	Introduction: The Mayaro virus (MAYV), which is an arbovirus closely related to the Chikungunya virus, causes a dengue-like acute illness that is endemic to Central and South America. We investigated the anti-MAYV activity of prostaglandin A1 (PGA1), a hormone which exhibits antiviral activity against both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) viruses. Further, we examined the effects of inducting the stress protein HSP70 following PGA1 treatment. Methods: Hep-2 cells infected with MAYV were treated with PGA1 (0.1-6μg/ml) 12h before infection and for different periods post-infection. Inhibition of viral replication inhibition was analyzed via viral titer determination, whereas the effect of PGA1 on viral morphogenesis was examined via transmission electron microscopy (TEM). Autoradiography (with 35S methionine labeling) and western blotting were used to assess the effect of PGA1 treatment on viral and cellular protein synthesis, and on HSP70 induction, respectively. Results: PGA1 strongly reduced viral replication in Hep-2 cells, particularly when added during the early stages of viral replication. Although PGA1 treatment inhibited viral replication by 95% at 24 hours post-infection (hpi), viral structural protein synthesis was inhibited only by 15%. TEM analysis suggested that PGA1 inhibited replication before viral morphogenesis. Western blot and densitometry analyses showed that PGA1 treatment increased HSP70 protein levels, although this was not detectable via autoradiography. Conclusions: PGA1 inhibits MAYV replication in Hep-2 cells at early stages of viral replication, prior to production of viral structural proteins, possibly via HSP70 induction.
MeSH term(s)	Alphavirus/drug effects ; Alphavirus/ultrastructure ; Animals ; Antiviral Agents/pharmacology ; Blotting, Western ; Cattle ; Cell Line ; Electrophoresis, Polyacrylamide Gel ; Epithelial Cells/ultrastructure ; Epithelial Cells/virology ; HSP70 Heat-Shock Proteins/pharmacology ; Humans ; Microscopy, Electron, Transmission ; Prostaglandins A/pharmacology ; Virus Replication/drug effects
Chemical Substances	Antiviral Agents ; HSP70 Heat-Shock Proteins ; Prostaglandins A ; prostaglandin A1 (VYR271N44P)
Language	English
Publishing date	2018-07-26
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	1038126-0
ISSN	1678-9849 ; 0037-8682
ISSN (online)	1678-9849
ISSN	0037-8682
DOI	10.1590/0037-8682-0235-2018
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Article ; Online: Microscopy analysis of Zika virus morphogenesis in mammalian cells.

Caldas, Lucio Ayres / Azevedo, Renata Campos / da Silva, Jerson Lima / de Souza, Wanderley

Scientific reports

2020 Volume 10, Issue 1, Page(s) 8370

Abstract: Zika virus (ZIKV) is an arbovirus that recently emerged in the Americas as an important pathogen mainly because of its expanded pathogenesis, and elevated tropism for neuronal cells, transposition across the placental barrier, and replication in ... ...

Abstract	Zika virus (ZIKV) is an arbovirus that recently emerged in the Americas as an important pathogen mainly because of its expanded pathogenesis, and elevated tropism for neuronal cells, transposition across the placental barrier, and replication in reproductive tract cells. Thus, transmission modes are eventually independent of an invertebrate vector, which is an atypical behavior for the flavivirus genus and indicates the need to study the replication of this virus in different cell types. Although ZIKV became a target for public health programs, the interaction of this flavivirus with the infected cell is still poorly understood. Herein, we analyzed the main stages of virus morphogenesis in mammalian cells, from establishment of the viroplasm-like zone to viral release from infected cells, using super-resolution fluorescence microscopy and electron microscopy. In addition, we compared this with other host cell types and other members of the Flaviviridae family that present a similar dynamic.
MeSH term(s)	Aedes ; Animals ; Cell Line ; Chlorocebus aethiops ; Electron Microscope Tomography ; Epithelial Cells/ultrastructure ; Epithelial Cells/virology ; Host Microbial Interactions ; Humans ; Macaca mulatta ; Microscopy, Fluorescence ; Morphogenesis ; Virus Release/physiology ; Virus Replication/physiology ; Zika Virus/growth & development ; Zika Virus/pathogenicity ; Zika Virus Infection/transmission ; Zika Virus Infection/virology
Language	English
Publishing date	2020-05-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-65409-y
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Article ; Online: Route of Zika virus infection in Aedes aegypti by transmission electron microscopy.

da Encarnação Sá-Guimarães, Thayane / Salles, Tiago Souza / Rocha Dos Santos, Carlucio / Moreira, Monica Ferreira / de Souza, Wanderley / Caldas, Lucio Ayres

BMC microbiology

2021 Volume 21, Issue 1, Page(s) 300

Abstract: Background: Zika fever has been a global health security threat, especially in the tropical and subtropical regions where most of the cases occur. The disease is caused by Zika virus (ZIKV), which belongs to the family Flaviviridae, genus Flavivirus. ... ...

Abstract	Background: Zika fever has been a global health security threat, especially in the tropical and subtropical regions where most of the cases occur. The disease is caused by Zika virus (ZIKV), which belongs to the family Flaviviridae, genus Flavivirus. The virus is transmitted by Aedes mosquitoes, mostly by Aedes aegypti, during its blood meal. In this study we present a descriptive analysis, by transmission electron microscopy (TEM), of ZIKV infection in A. aegypti elected tissues at the 3rd day of infection. ZIKV vertical transmission experiments by oral infection were conducted to explore an offspring of natural infection. Results: Gut and ovary tissues harbored a higher number of viral particles. The ZIKV genome was also detected, by RT-qPCR technique, in the organism of orally infected female mosquitoes and in their eggs laid. Conclusions: The data obtained suggest that the ovary is an organ susceptible to be infected with ZIKV and that virus can be transmitted from mother to a fraction of the progeny.
MeSH term(s)	Aedes/virology ; Animals ; Female ; Intestines/virology ; Microscopy, Electron, Transmission ; Mosquito Vectors/virology ; Ovary/virology ; Ovum/virology ; RNA, Viral/genetics ; Virion/ultrastructure ; Zika Virus/physiology ; Zika Virus/ultrastructure ; Zika Virus Infection/transmission ; Zika Virus Infection/virology
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-10-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1471-2180
ISSN (online)	1471-2180
DOI	10.1186/s12866-021-02366-0
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Article ; Online: Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro.

Caldas, Lucio Ayres / Carneiro, Fabiana Avila / Monteiro, Fabio Luis / Augusto, Ingrid / Higa, Luiza Mendonça / Miranda, Kildare / Tanuri, Amilcar / de Souza, Wanderley

Biology of the cell

2021 Volume 113, Issue 6, Page(s) 281–293

Abstract: Background information: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to ... ...

Abstract	Background information: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to investigate the main structural alterations in the cytoplasm of Vero cells infected with a SARS-CoV-2 isolate from São Paulo state (Brazil). Results: Different membranous structures derived from the zippered endoplasmic reticulum were observed along with virus assembly through membrane budding. Also, we demonstrated the occurrence of annulate lamellae in the cytoplasm of infected cells and the presence of virus particles in the perinuclear space. Conclusions and significance: This study contributes to a better understanding of the cell biology of SARS-CoV-2 and the mechanisms of the interaction of the virus with the host cell that promote morphological changes, recruitment of organelles and cell components, in a context of a virus-induced membrane remodelling.
MeSH term(s)	Animals ; COVID-19 ; Chlorocebus aethiops ; Electron Microscope Tomography ; Endoplasmic Reticulum/ultrastructure ; Endoplasmic Reticulum/virology ; Humans ; Intracellular Membranes/ultrastructure ; Intracellular Membranes/virology ; Microscopy, Electron, Transmission ; Nuclear Envelope/ultrastructure ; Nuclear Envelope/virology ; SARS-CoV-2/growth & development ; SARS-CoV-2/ultrastructure ; Vero Cells ; Virus Assembly ; Virus Replication
Language	English
Publishing date	2021-03-03
Publishing country	England
Document type	Journal Article
ZDB-ID	245745-3
ISSN	1768-322X ; 0399-0311 ; 0248-4900
ISSN (online)	1768-322X
ISSN	0399-0311 ; 0248-4900
DOI	10.1111/boc.202000146
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Article: Chemical composition and anti-Mayaro virus activity of

Salles, Tiago S / Meneses, Marcelo D F / Yamamoto, Kristie A / Sá-Guimarães, Thayane E / Caldas, Lucio Ayres / Silva, Jessica H S / da Silva Ferreira, Polianna / Amaral, Ana Claudia F / Ventura, José A / Azevedo, Renata Campos / Kuster, Ricardo M / Soares, Márcia R / Ferreira, Davis F

Virusdisease

2021 Volume 32, Issue 3, Page(s) 526–534

Abstract: Brazilian traditional medicine has explored the antiviral properties of many plant extracts, including those from the Brazilian pepper tree, : Supplementary information: The online version contains supplementary material available at 10.1007/s13337- ... ...

Abstract	Brazilian traditional medicine has explored the antiviral properties of many plant extracts, including those from the Brazilian pepper tree, Supplementary information: The online version contains supplementary material available at 10.1007/s13337-021-00698-z.
Language	English
Publishing date	2021-06-04
Publishing country	India
Document type	Journal Article
ZDB-ID	2846993-8
ISSN	2347-3517 ; 2347-3584
ISSN (online)	2347-3517
ISSN	2347-3584
DOI	10.1007/s13337-021-00698-z
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