Article ; Online: Glucosylceramide in T cells regulates the pathology of inflammatory bowel disease.
Biochemical and biophysical research communications
2022 Volume 599, Page(s) 24–30
Abstract: ... by excessive activation of T cells. Glycosphingolipids (GSLs) are composed of lipid rafts in cellular membranes ... and T cells from the spleen. Furthermore, the knockdown of Ugcg in T cells exacerbated ... nanoparticles prevented DSS-induced colitis. These results suggested that GlcCer in T cells is involved ...
Abstract | Inflammatory bowel disease (IBD) is a chronic inflammatory disease in the colon characterized by excessive activation of T cells. Glycosphingolipids (GSLs) are composed of lipid rafts in cellular membranes, and their content is linked to immune cell function. In the present study, we investigated the involvement of GSLs in IBD. Microarray data showed that in IBD patients, the expression of only UDP-glucose ceramide glucosyltransferase (UGCG) decreased among the GSLs synthases. Ad libitum access to dextran sulfate sodium (DSS) resulted in decreased UGCG and glucosylceramide (GlcCer) content in mesenteric lymph nodes and T cells from the spleen. Furthermore, the knockdown of Ugcg in T cells exacerbated the pathogenesis of colitis, which was accompanied by a decrease in Treg levels. Treatment with GlcCer nanoparticles prevented DSS-induced colitis. These results suggested that GlcCer in T cells is involved in the pathogenesis of IBD. Furthermore, GlcCer nanoparticles are a potential efficacious therapeutic target for IBD patients. |
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MeSH term(s) | Animals ; Colitis/chemically induced ; Colitis/drug therapy ; Colitis/pathology ; Dextran Sulfate/toxicity ; Disease Models, Animal ; Glucosylceramides/administration & dosage ; Glucosylceramides/genetics ; Glucosylceramides/metabolism ; Glucosyltransferases/genetics ; Glucosyltransferases/metabolism ; Humans ; Inflammatory Bowel Diseases/metabolism ; Inflammatory Bowel Diseases/pathology ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Nanoparticles/administration & dosage ; Nanoparticles/chemistry ; T-Lymphocytes/metabolism ; T-Lymphocytes/pathology ; Mice |
Chemical Substances | Glucosylceramides ; Dextran Sulfate (9042-14-2) ; Glucosyltransferases (EC 2.4.1.-) ; ceramide glucosyltransferase (EC 2.4.1.80) |
Language | English |
Publishing date | 2022-02-03 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 205723-2 |
ISSN | 1090-2104 ; 0006-291X ; 0006-291X |
ISSN (online) | 1090-2104 ; 0006-291X |
ISSN | 0006-291X |
DOI | 10.1016/j.bbrc.2022.02.004 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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