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  1. Article: Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader.

    Marofi, Hossein / Rafati, Adele / Gill, Pooria

    Journal of medical signals and sensors

    2023  Volume 13, Issue 2, Page(s) 136–143

    Abstract: Background: We described here an aptamer-based magnetic nanoprobe for measuring the amount of chloramphenicol (CAP) in milk.: Methods: The nanoprobe presented in this method consists of a magnetic nanoparticle conjugated to a specific CAP aptamer. If ...

    Abstract Background: We described here an aptamer-based magnetic nanoprobe for measuring the amount of chloramphenicol (CAP) in milk.
    Methods: The nanoprobe presented in this method consists of a magnetic nanoparticle conjugated to a specific CAP aptamer. If the target is detected in the sample, the nanoprobe binds to it, and the aptamer forms a G-quadruplex structure. This structure mimics the peroxidase activity in the presence of the hemin cofactor. If tetramethylbenzidine is added to the sample containing the nanoprobe, a blue color light is observed. After adding a stop reagent solution, the color produced is measured by a microplate reader and a portable meter.
    Results: This study proves a 99% positive linear relationship between the microplate reader's results and the portable meter results.
    Conclusion: Conjugation of the aptamer to magnetic nanoparticles and applying magnetic separation operations change the nanoprobe performance by 11% for both mentioned devices.
    Language English
    Publishing date 2023-05-29
    Publishing country India
    Document type Journal Article
    ZDB-ID 2651622-6
    ISSN 2228-7477
    ISSN 2228-7477
    DOI 10.4103/jmss.jmss_177_21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Fluorescent Detection of Methicillin Resistant

    Aliasgharian, Aily / Gill, Pooria / Ahanjan, Mohammad / Rafati, Adele

    Avicenna journal of medical biotechnology

    2022  Volume 14, Issue 1, Page(s) 79–88

    Abstract: Background: Methicillin Resistance : Methods: Using molecular diagnostic methods are important for identification of : Results: Obtained results indicated that % sensitivity of QD-LAMP was 86.66% for detection of : Conclusion: The results ... ...

    Abstract Background: Methicillin Resistance
    Methods: Using molecular diagnostic methods are important for identification of
    Results: Obtained results indicated that % sensitivity of QD-LAMP was 86.66% for detection of
    Conclusion: The results suggested that the QD-LAMP assay was easy to operate and could be used for detection of
    Language English
    Publishing date 2022-04-21
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2520683-7
    ISSN 2008-4625 ; 2008-2835
    ISSN (online) 2008-4625
    ISSN 2008-2835
    DOI 10.18502/ajmb.v14i1.8546
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  3. Article ; Online: The Role of Advanced Technologies against COVID-19: Prevention, Detection, and Treatments.

    Hasanzadeh, Elham / Rafati, Adele / Tamijani, Seyedeh Masoumeh Seyed Hosseini / Rafaiee, Raheleh / Golchin, Ali / Abasi, Mozhgan

    Current stem cell research & therapy

    2022  Volume 18, Issue 6, Page(s) 800–828

    Abstract: Concurrent with the global outbreak of COVID-19, the race began among scientists to generate effective therapeutics for the treatment of COVID-19. In this regard, advanced technology such as nanotechnology, cell-based therapies, tissue engineering and ... ...

    Abstract Concurrent with the global outbreak of COVID-19, the race began among scientists to generate effective therapeutics for the treatment of COVID-19. In this regard, advanced technology such as nanotechnology, cell-based therapies, tissue engineering and regenerative medicine, nerve stimulation and artificial intelligence (AI) are attractive because they can offer new solutions for the prevention, diagnosis and treatment of COVID-19. Nanotechnology can design rapid and specific tests with high sensitivity for detecting infection and synthases new drugs and vaccines based on nanomaterials to directly deliver the intended antiviral agent to the desired site in the body and also provide new surfaces that do not allow virus adhesion. Mesenchymal stem cells and exosomes secreted from them apply in regenerative medicine and regulate inflammatory responses. Cell therapy and tissue engineering are combined to repair or substitute damaged tissues or cells. Tissue engineering using biomaterials, cells, and signaling molecules can develop new therapeutic and diagnostic platforms and help scientists fight viral diseases. Nerve stimulation technology can augment body's natural ability to modulate the inflammatory response and inhibit pro-inflammatory cytokines and consequently suppress cytokine storm. People can access free online health counseling services through AI and it helps very fast for screening and diagnosis of COVID-19 patients. This study is aimed first to give brief information about COVID-19 and the epidemiology of the disease. After that, we highlight important developments in the field of advanced technologies relevant to the prevention, detection, and treatment of the current pandemic.
    MeSH term(s) Humans ; COVID-19/prevention & control ; SARS-CoV-2 ; Artificial Intelligence ; Technology ; Nanotechnology
    Language English
    Publishing date 2022-12-06
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2251937-3
    ISSN 2212-3946 ; 1574-888X
    ISSN (online) 2212-3946
    ISSN 1574-888X
    DOI 10.2174/1574888X18666221221123505
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: DNA Nanotubes Coupled with Magnetic Nanoparticles as a Platform for Colorimetric Biosensors

    Adele Rafati / Ali Zarrabi / Pooria Gill

    Nanomedicine Research Journal, Vol 5, Iss 1, Pp 63-

    2020  Volume 74

    Abstract: This study describes the fabrication techniques for two forms of magnetic DNA nanotubes (MDNTs) and their applications as platforms for developing colorimetric assays. The first form of MDNTs was DNTs filled-up with magnetic nanoparticles (MNPs) and the ... ...

    Abstract This study describes the fabrication techniques for two forms of magnetic DNA nanotubes (MDNTs) and their applications as platforms for developing colorimetric assays. The first form of MDNTs was DNTs filled-up with magnetic nanoparticles (MNPs) and the second one was DNTs arayed with MNPs on their extrior surfaces. Then the both forms of MDNTs were employed as platforms for attaching a specific insulin aptamer. The sensitivity and accuracy of the insulin measurement using both platforms were studied and compared with enzyme-linked immunosorbent assay (ELISA) as the standard method for the measurement of insulin in cliniccal laboratories. Applying the magnetic field to MDNTs led to enhance the ability of insulin capturing by the aptamer array in serum and subsequently by removing unspecified contents led to precised detection. For specific detection of insulin and its measurement in this study, a G-rich DNA aptamer with HRP-mimicking activity was used that simulated the peroxidase performance when the insulin was trapped with the aptamer. The presence of MDNTs made a high flexibility, a greater convergence of the connectivity to superficial aptamers, and hence the availability of these aptamers to increase the target molecules and subsequently increased the sensitivity of the measurements. This colorimetric method can be used as a novel biosensores for detection of any target molecules.
    Keywords magnetic dna nanotubes (mdnts) ; insulin ; g-quadruplex aptamer ; hrp-mimicking activity ; Medicine ; R
    Subject code 500
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher Iranian Society of Nanomedicine
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Ultrastructural characterizations of DNA nanotubes using scanning tunneling and atomic force microscopes.

    Rafati, Adele / Gill, Pooria

    Journal of microscopy and ultrastructure

    2015  Volume 4, Issue 1, Page(s) 1–5

    Abstract: The potential applications of scanning tunneling microscopy and atomic force microscopy for the characterizations of DNA nanotubes in nanoscale have been described here. The nanotubes were designed using the Cadnano software according to ... ...

    Abstract The potential applications of scanning tunneling microscopy and atomic force microscopy for the characterizations of DNA nanotubes in nanoscale have been described here. The nanotubes were designed using the Cadnano software according to M
    Language English
    Publishing date 2015-09-28
    Publishing country India
    Document type Journal Article
    ISSN 2213-8803
    ISSN (online) 2213-8803
    DOI 10.1016/j.jmau.2015.08.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Fabrication of DNA nanotubes with an array of exterior magnetic nanoparticles.

    Rafati, Adele / Zarrabi, Ali / Gill, Pooria

    Materials science & engineering. C, Materials for biological applications

    2017  Volume 79, Page(s) 216–220

    Abstract: Described here a methodology for arraying of magnetic nanoparticles (MNPs) on the surface of DNA nanotubes (DNTs). Positioning of magnetic nanoparticles at exterior surface of DNTs were shaped after self-assembling of oligonucleotide staples within an ... ...

    Abstract Described here a methodology for arraying of magnetic nanoparticles (MNPs) on the surface of DNA nanotubes (DNTs). Positioning of magnetic nanoparticles at exterior surface of DNTs were shaped after self-assembling of oligonucleotide staples within an M13mp18 DNA scaffold via an origami process. The staples were partially labeled with biotin to be arrayed at the surface of DNTs. Gel retardation assay of the DNTs carrying magnetic nanoparticles indicated a reversely behavioral electrophoretic movement in comparison to the nanotubes have been demonstrated previously. Also, high resolution transmission electron microscopy confirmed positioning magnetic nanoparticles at the exterior surface of DNTs, correctly. Ultrastructural characteristics of these DNA nanotubes using atomic force microscopy demonstrated topographic heights on their surfaces formed through positioning of magnetic nanoparticles outside the tubules. This nanoarchitecture would be potential for multiple arraying of nanoparticles that those be useful as functionalized chimeric nanocarriers for developing novel nanodrugs and nanobiosensors.
    Language English
    Publishing date 2017-10-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2012160-X
    ISSN 1873-0191 ; 0928-4931
    ISSN (online) 1873-0191
    ISSN 0928-4931
    DOI 10.1016/j.msec.2017.05.044
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