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  1. Article ; Online: Complex genetics of Alport and Goodpasture syndromes.

    Tryggvason, Karl

    Nature reviews. Nephrology

    2021  Volume 17, Issue 10, Page(s) 635–636

    MeSH term(s) Anti-Glomerular Basement Membrane Disease/genetics ; Humans ; Mutation ; Nephritis, Hereditary/genetics
    Language English
    Publishing date 2021-10-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2490366-8
    ISSN 1759-507X ; 1759-5061
    ISSN (online) 1759-507X
    ISSN 1759-5061
    DOI 10.1038/s41581-021-00451-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: 70 K type IV collagenase (gelatinase).

    Tryggvason, K / Huhtala, P / Höyhtya, M / Hujanen, E / Hurskainen, T

    Matrix (Stuttgart, Germany). Supplement

    1992  Volume 1, Page(s) 45–50

    Abstract: Type IV collagenase (gelatinase) is a 70,000 dalton neutral metalloproteinase that specifically cleaves type IV collagen in addition to degrading denatured collagen (gelatin). It is secreted in a latent proenzyme form that is converted proteolytically in ...

    Abstract Type IV collagenase (gelatinase) is a 70,000 dalton neutral metalloproteinase that specifically cleaves type IV collagen in addition to degrading denatured collagen (gelatin). It is secreted in a latent proenzyme form that is converted proteolytically in the extracellular space to a 62,000 dalton active enzyme. The primary structure, enzymatic properties as well as gene structure, demonstrate that type IV collagenase is closely related with the other well characterized metalloproteinases, interstitial collagenase and stromelysin. However, the structure of type IV collagenase differs from the others in that it is larger and contains three internal repeats that resemble the type II domains of fibronectin. Also, initial characterization of the promoter region of the gene indicates that its regulation differs from the other proteinase genes. Type IV collagenase is presumably required for the normal turnover of basement membranes. Augmented activity is linked with the invasive potential of tumor cells and the enzyme is believed to play a major role in the penetration of basement membranes by metastatic cells. Measurements of enzyme activity and mRNA levels as well as immunostaining of a variety of tumor cells and tissues suggest that assays for the enzyme may have value in the follow-up of malignant growth.
    MeSH term(s) Amino Acid Sequence ; Animals ; Base Sequence ; Basement Membrane/metabolism ; Collagenases/chemistry ; Collagenases/genetics ; Collagenases/immunology ; Collagenases/physiology ; Genes ; Humans ; Matrix Metalloproteinase 9 ; Molecular Sequence Data ; Molecular Weight ; Neoplasm Invasiveness ; Neoplasm Proteins/physiology ; Rabbits ; Rats ; Sequence Alignment ; Substrate Specificity
    Chemical Substances Neoplasm Proteins ; Collagenases (EC 3.4.24.-) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language English
    Publishing date 1992
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ISSN 0940-1199
    ISSN 0940-1199
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online: Molecular Pathology and Genetics of Alport Syndrome

    Tryggvason, K.

    (Contributions to Nephrology ; Vol.117)

    1996  

    Series title Contributions to Nephrology ; Vol.117
    Keywords Genetics ; Nephrology ; Pathology ; Epidemiology ; Internal Medicine ; Molecular Biology ; Pediatrics ; Surgery ; Transplantation ; Urology
    Language English
    Size 1 Online-Ressource (VIII + 200 S.)
    Publisher S. Karger
    Publishing place Basel
    Document type Book ; Online
    HBZ-ID HT019542640
    ISBN 978-3-318-03381-6 ; 3-318-03381-2
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  4. Article: Structure of the human 70 K type IV collagenase gene and assignment of the gene to the q21 region of chromosome 16.

    Huhtala, P / Chow, L / Shows, T / Tryggvason, K

    Matrix (Stuttgart, Germany). Supplement

    1992  Volume 1, Page(s) 84

    MeSH term(s) Chromosome Mapping ; Chromosomes, Human, Pair 16 ; Collagenases/genetics ; Genes ; Humans ; Matrix Metalloproteinase 9
    Chemical Substances Collagenases (EC 3.4.24.-) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language English
    Publishing date 1992
    Publishing country Germany
    Document type Journal Article
    ISSN 0940-1199
    ISSN 0940-1199
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Chemically defined and xenogeneic-free culture method for human epidermal keratinocytes on laminin-based matrices.

    Tjin, Monica Suryana / Chua, Alvin Wen Choong / Tryggvason, Karl

    Nature protocols

    2020  Volume 15, Issue 2, Page(s) 694–711

    Abstract: The basal keratinocyte progenitor cells in cultured epithelial autografts (CEAs) regenerate human epidermis after transplantation, a curative therapy for severe burns and, recently, diseases with epidermal loss, such as junctional epidermolysis bullosa ( ... ...

    Abstract The basal keratinocyte progenitor cells in cultured epithelial autografts (CEAs) regenerate human epidermis after transplantation, a curative therapy for severe burns and, recently, diseases with epidermal loss, such as junctional epidermolysis bullosa (EB). Although a culturing technique for skin keratinocytes was developed four decades ago, the xenogeneic nature of that conventional CEA culture system restricts its use to the treatment of critical and life-threatening cases, such as severe burns on >30% of total body surface area and EB. In the present protocol, we describe how to implement a defined, xeno-free culture system that supports long-term ex vivo expansion of functional human epidermal keratinocytes. Skin-specific basement membrane proteins called laminins play important roles in the maintenance of phenotypic integrity and in supporting the survival of keratinocytes that are adhered to them. This fully human keratinocyte culture system is 'regulatory friendly' and increases the potential of epithelial cellular therapy, which can be expanded to treat less severe burns and other skin defects, such as chronic diabetic wounds. It takes between 7 and 14 d to obtain an initial culture. Conservatively, a secondary culture from the primary culture can be expanded up to 20-fold within 4-5 d once cells reach confluency.
    MeSH term(s) 3T3 Cells ; Animals ; Basement Membrane/metabolism ; Cell Culture Techniques/methods ; Epidermis/metabolism ; Feeder Cells/cytology ; Humans ; Keratinocytes/cytology ; Keratinocytes/metabolism ; Laminin/metabolism ; Mice
    Chemical Substances Laminin
    Language English
    Publishing date 2020-01-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2244966-8
    ISSN 1750-2799 ; 1754-2189
    ISSN (online) 1750-2799
    ISSN 1754-2189
    DOI 10.1038/s41596-019-0270-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Variable stiffness foot design and validation.

    Lecomte, Christophe / Ármannsdóttir, Anna Lára / Starker, Felix / Tryggvason, Heimir / Briem, Kristin / Brynjolfsson, Sigurður

    Journal of biomechanics

    2021  Volume 122, Page(s) 110440

    Abstract: Energy storing and returning prosthetic feet are commonly prescribed. Research has demonstrated advantages to use these types of prosthetic feet. However, their stiffness in the sagittal plane is fixed and cannot adapt to different walking tasks and user ...

    Abstract Energy storing and returning prosthetic feet are commonly prescribed. Research has demonstrated advantages to use these types of prosthetic feet. However, their stiffness in the sagittal plane is fixed and cannot adapt to different walking tasks and user preference. In this paper, we propose a novel prosthetic foot design capable of modulating its stiffness in the sagittal plane. The Variable Stiffness Ankle unit (VSA) is mounted on a commercially available prosthetic foot. The stiffness of the foot is adjusted with a lightweight servo motor controlled wirelessly. The stiffness change is accomplished by moving the supports points on the glass fiber leaf spring of the VSA ankle unit. We described the design and characterized changes in ankle stiffness using a mechanical test bench. A novel method was used to capture mechanical test data using a six degree of freedom load cell, allowing us to contrast mechanical and biomechanical data. A transtibial unilateral amputee performed level ground walking on an instrumented treadmill. The VSA prosthetic foot exhibited ankle stiffness change in the mechanical test bench. Ankle stiffness changes were also confirmed during the biomechanical analysis. Future work will involve additional subjects. The VSA prosthetic foot could improve user satisfaction and help prosthetist to fine tune prosthetic feet during fittings.
    MeSH term(s) Amputees ; Artificial Limbs ; Biomechanical Phenomena ; Foot ; Gait ; Prosthesis Design ; Walking
    Language English
    Publishing date 2021-04-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218076-5
    ISSN 1873-2380 ; 0021-9290
    ISSN (online) 1873-2380
    ISSN 0021-9290
    DOI 10.1016/j.jbiomech.2021.110440
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Structural stability and oxidation resistance of amorphous TaSi-based ternary alloy coatings

    D.I. Snorrason / S.M. Adalsteinsson / T.K. Tryggvason / D. Dagbjartsson / A.S. Ingason / F. Magnus

    Journal of Non-Crystalline Solids: X, Vol 18, Iss , Pp 100183- (2023)

    2023  

    Abstract: Amorphous metal coatings have great potential for corrosion protection but finding alloy compositions which form a stable amorphous structure can be an overwhelming task. We use combinatorial magnetron sputtering and X-ray analysis to map out the phase ... ...

    Abstract Amorphous metal coatings have great potential for corrosion protection but finding alloy compositions which form a stable amorphous structure can be an overwhelming task. We use combinatorial magnetron sputtering and X-ray analysis to map out the phase space of TaSiM (M = Al, Cr, Fe, Ti) alloys in order to identify amorphous compositions. Atomic percentages of above 10–15 at.% of each constituent yield amorphous coatings in all four systems. TaSiAl coatings are stable when annealed in air up to and including 550 °C whereas TaSiFe, TaSiCr and TaSiTi remain amorphous up to and including 750 °C. In particular, Ta35Si15Cr50 is almost unchanged at that temperature, and has a stable surface oxide shell less than 20 nm in thickness at 650 °C. The stability of these materials at high temperatures means that they could be suitable as anti-corrosion coatings in high temperature applications.
    Keywords Amorphous ; Thin films ; Metallic glass ; Corrosion resistance ; Crystallization temperature ; Combinatorial ; Materials of engineering and construction. Mechanics of materials ; TA401-492 ; Chemistry ; QD1-999
    Subject code 669
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Utilization and outcomes of tracheostomies in the intensive care unit in Iceland in 2007-2020: A descriptive study.

    Kristinsdottir, Eyrun A / Sigvaldason, Kristinn / Karason, Sigurbergur / Jonasdottir, Rannveig J / Bodvarsdottir, Regina / Olafsson, Oddur / Tryggvason, Geir / Gudbjartsson, Tomas / Sigurdsson, Martin I

    Acta anaesthesiologica Scandinavica

    2022  Volume 66, Issue 8, Page(s) 996–1002

    Abstract: Background: Tracheostomies are commonly utilized in ICU patients due to prolonged mechanical ventilation, upper airway obstruction, or surgery in the face/neck region. However, practices regarding the timing of placement and utilization vary. This study ...

    Abstract Background: Tracheostomies are commonly utilized in ICU patients due to prolonged mechanical ventilation, upper airway obstruction, or surgery in the face/neck region. However, practices regarding the timing of placement and utilization vary. This study provides a nationwide overview of tracheostomy utilization and outcomes in the ICU over a 14-year period.
    Methods: A retrospective study including all patients that received a tracheostomy during their ICU stay in Iceland between 2007 and 2020. Data were retrieved from hospital records on admission cause, comorbidities, indication for tracheostomy insertion, duration of mechanical ventilation before and after tracheostomy placement, extubation attempts, complications, length of ICU and hospital stay and survival. Descriptive statistics were provided, and survival analysis was performed using Cox regression.
    Results: A total of 336 patients (median age 64 years, 33% females) received a tracheostomy during the study period. The most common indication for tracheostomy insertion was respiratory failure, followed by neurological disorders. The median duration of mechanical ventilation prior to tracheostomy insertion was 9 days and at least one extubation had been attempted in 35% of the cases. Percutaneous tracheostomies were 32%. The overall rate of complications was 25% and the most common short-term complication was bleeding (5%). In-hospital mortality was 33%. The one- and five-year survival rate was 60% and 44%, respectively.
    Conclusions: We describe a whole-nation practice of tracheostomies. A notable finding is the relatively low rate of extubation attempts prior to tracheostomy insertion. Future work should focus on standardization of assessing the need for tracheostomy and the role of extubation attempts prior to tracheostomy placement.
    MeSH term(s) Female ; Humans ; Iceland/epidemiology ; Intensive Care Units ; Length of Stay ; Male ; Middle Aged ; Respiration, Artificial ; Retrospective Studies ; Tracheostomy
    Language English
    Publishing date 2022-06-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 80002-8
    ISSN 1399-6576 ; 0001-5172
    ISSN (online) 1399-6576
    ISSN 0001-5172
    DOI 10.1111/aas.14105
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Skin mesenchymal niches maintain and protect AML-initiating stem cells.

    Sandhow, Lakshmi / Cai, Huan / Leonard, Elory / Xiao, Pingnan / Tomaipitinca, Luana / Månsson, Alma / Kondo, Makoto / Sun, Xiaoyan / Johansson, Anne-Sofie / Tryggvason, Karl / Kasper, Maria / Järås, Marcus / Qian, Hong

    The Journal of experimental medicine

    2023  Volume 220, Issue 10

    Abstract: Leukemia cutis or leukemic cell infiltration in skin is one of the common extramedullary manifestations of acute myeloid leukemia (AML) and signifies a poorer prognosis. However, its pathogenesis and maintenance remain understudied. Here, we report ... ...

    Abstract Leukemia cutis or leukemic cell infiltration in skin is one of the common extramedullary manifestations of acute myeloid leukemia (AML) and signifies a poorer prognosis. However, its pathogenesis and maintenance remain understudied. Here, we report massive AML cell infiltration in the skin in a transplantation-induced MLL-AF9 AML mouse model. These AML cells could regenerate AML after transplantation. Prospective niche characterization revealed that skin harbored mesenchymal progenitor cells (MPCs) with a similar phenotype as BM mesenchymal stem cells. These skin MPCs protected AML-initiating stem cells (LSCs) from chemotherapy in vitro partially via mitochondrial transfer. Furthermore, Lama4 deletion in skin MPCs promoted AML LSC proliferation and chemoresistance. Importantly, more chemoresistant AML LSCs appeared to be retained in Lama4-/- mouse skin after cytarabine treatment. Our study reveals the characteristics and previously unrecognized roles of skin mesenchymal niches in maintaining and protecting AML LSCs during chemotherapy, meriting future exploration of their impact on AML relapse.
    MeSH term(s) Animals ; Mice ; Prospective Studies ; Stem Cells ; Skin ; Leukemia, Myeloid, Acute ; Mesenchymal Stem Cells
    Language English
    Publishing date 2023-07-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218343-2
    ISSN 1540-9538 ; 0022-1007
    ISSN (online) 1540-9538
    ISSN 0022-1007
    DOI 10.1084/jem.20220953
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Nephrin: role in normal kidney and in disease.

    Tryggvason, K

    Advances in nephrology from the Necker Hospital

    2001  Volume 31, Page(s) 221–234

    MeSH term(s) Humans ; Kidney/physiology ; Kidney/physiopathology ; Kidney Diseases/genetics ; Kidney Diseases/physiopathology ; Membrane Proteins ; Proteins/genetics ; Proteins/metabolism
    Chemical Substances Membrane Proteins ; Proteins ; nephrin
    Language English
    Publishing date 2001
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 121702-1
    ISSN 0084-5957
    ISSN 0084-5957
    Database MEDical Literature Analysis and Retrieval System OnLINE

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