LIVIVO - Search results -

Search results

Result 1 - 10 of total 85

Search options

Article ; Online: Parkin mRNA Expression Levels in Peripheral Blood Mononuclear Cells in Parkin-Related Parkinson's Disease.

Papagiannakis, Nikolaos / Liu, Hui / Koros, Christos / Simitsi, Athina-Maria / Stamelou, Maria / Maniati, Matina / Buena-Atienza, Elena / Kartanou, Chrysoula / Karadima, Georgia / Makrythanasis, Periklis / Vatsellas, Giannis / Valente, Enza Maria / Gasser, Thomas / Stefanis, Leonidas

Movement disorders : official journal of the Movement Disorder Society

2024 Volume 39, Issue 4, Page(s) 715–722

Abstract: Introduction: Pathogenic variants in parkin (PRKN gene) are the second most prevalent known monogenic cause of Parkinson's disease (PD). How monoallelic or biallelic pathogenic variants in the PRKN gene may affect its transcription in patient-derived ... ...

Abstract	Introduction: Pathogenic variants in parkin (PRKN gene) are the second most prevalent known monogenic cause of Parkinson's disease (PD). How monoallelic or biallelic pathogenic variants in the PRKN gene may affect its transcription in patient-derived biological material has not been systematically studied. Methods: PRKN mRNA expression levels were measured with real-time polymerase chain reaction (RT-PCR) in peripheral blood mononuclear cells (PBMCs). PBMCs were derived from PRKN-mutated PD patients (PRKN-PD) (n = 12), sporadic PD (sPD) (n = 21) and healthy controls (n = 21). Six of the PRKN-PD patients were heterozygous, four were compound heterozygous, and two were homozygous for PRKN variants. Results: A statistically significant decrease in PRKN expression levels was present, compared to healthy controls and sPD, in heterozygous (P = 0.019 and 0.031 respectively) and biallelic (P < 0.001 for both) PRKN-PD. PRKN expression levels in biallelic PD patients were uniformly very low and were reduced, albeit not significantly, compared to heterozygotes. Based on receiver operating characteristic analysis, low PRKN expression levels were a sensitive and extremely specific indicator for the presence of PRKN pathogenic variants. Conclusions: Assessment of PRKN mRNA levels in PBMCs may be a useful way to screen for biallelic pathogenic variants in the PRKN gene. Suspicion for certain variants in a heterozygous state may also be raised based on low PRKN mRNA levels. © 2024 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
MeSH term(s)	Humans ; Ubiquitin-Protein Ligases/genetics ; Parkinson Disease/genetics ; Parkinson Disease/blood ; Leukocytes, Mononuclear/metabolism ; Male ; Female ; RNA, Messenger/metabolism ; Middle Aged ; Aged ; Adult ; Mutation
Chemical Substances	parkin protein (EC 2.3.2.27) ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; RNA, Messenger
Language	English
Publishing date	2024-02-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	607633-6
ISSN	1531-8257 ; 0885-3185
ISSN (online)	1531-8257
ISSN	0885-3185
DOI	10.1002/mds.29739
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2555: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 238: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: ACSNI: An unsupervised machine-learning tool for prediction of tissue-specific pathway components using gene expression profiles.

Anene, Chinedu Anthony / Khan, Faraz / Bewicke-Copley, Findlay / Maniati, Eleni / Wang, Jun

Patterns (New York, N.Y.)

2021 Volume 2, Issue 6, Page(s) 100270

Abstract: Determining the tissue- and disease-specific circuit of biological pathways remains a fundamental goal of molecular biology. Many components of these biological pathways still remain unknown, hindering the full and accurate characterization of biological ...

Abstract	Determining the tissue- and disease-specific circuit of biological pathways remains a fundamental goal of molecular biology. Many components of these biological pathways still remain unknown, hindering the full and accurate characterization of biological processes of interest. Here we describe ACSNI, an algorithm that combines prior knowledge of biological processes with a deep neural network to effectively decompose gene expression profiles (GEPs) into multi-variable pathway activities and identify unknown pathway components. Experiments on public GEP data show that ACSNI predicts cogent components of mTOR, ATF2, and HOTAIRM1 signaling that recapitulate regulatory information from genetic perturbation and transcription factor binding datasets. Our framework provides a fast and easy-to-use method to identify components of signaling pathways as a tool for molecular mechanism discovery and to prioritize genes for designing future targeted experiments (https://github.com/caanene1/ACSNI).
Language	English
Publishing date	2021-06-11
Publishing country	United States
Document type	Journal Article
ISSN	2666-3899
ISSN (online)	2666-3899
DOI	10.1016/j.patter.2021.100270
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Differentially Expressed Circular RNAs in Peripheral Blood Mononuclear Cells of Patients with Parkinson's Disease.

Ravanidis, Stylianos / Bougea, Anastasia / Karampatsi, Dimitra / Papagiannakis, Nikolaos / Maniati, Matina / Stefanis, Leonidas / Doxakis, Epaminondas

Movement disorders : official journal of the Movement Disorder Society

2021 Volume 36, Issue 5, Page(s) 1170–1179

Abstract: Background: New noninvasive and affordable molecular approaches that will complement current practices and increase the accuracy of Parkinson's disease (PD) diagnosis are urgently needed. Circular RNAs (circRNAs) are stable noncoding RNAs that ... ...

Abstract	Background: New noninvasive and affordable molecular approaches that will complement current practices and increase the accuracy of Parkinson's disease (PD) diagnosis are urgently needed. Circular RNAs (circRNAs) are stable noncoding RNAs that accumulate with aging in neurons and are increasingly shown to regulate all aspects of neuronal development and function. Objectives: Τhe aims of this study were to identify differentially expressed circRNAs in blood mononuclear cells of patients with idiopathic PD and explore the competing endogenous RNA networks affected. Methods: Eighty-seven circRNAs were initially selected based on relatively high gene expression in the human brain. More than half of these were readily detectable in blood mononuclear cells using real-time reverse transcription-polymerase chain reaction. Comparative expression analysis was then performed in blood mononuclear cells from 60 control subjects and 60 idiopathic subjects with PD. Results: Six circRNAs were significantly down-regulated in patients with PD. The classifier that best distinguished PD consisted of four circRNAs with an area under the curve of 0.84. Cross-linking immunoprecipitation-sequencing data revealed that the RNA-binding proteins bound by most of the deregulated circRNAs include the neurodegeneration-associated FUS, TDP43, FMR1, and ATXN2. MicroRNAs predicted to be sequestered by most deregulated circRNAs have the Gene Ontology categories "protein modification" and "transcription factor activity" mostly enriched. Conclusions: This is the first study that identifies specific circRNAs that may serve as diagnostic biomarkers for PD. Because they are highly expressed in the brain and are derived from genes with essential brain functions, they may also hint on the PD pathways affected. © 2021 Biomedical Research Foundation, Academy of Athens. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
MeSH term(s)	Gene Ontology ; Humans ; Leukocytes, Mononuclear ; MicroRNAs/genetics ; Parkinson Disease/genetics ; RNA, Circular
Chemical Substances	MicroRNAs ; RNA, Circular
Language	English
Publishing date	2021-01-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	607633-6
ISSN	1531-8257 ; 0885-3185
ISSN (online)	1531-8257
ISSN	0885-3185
DOI	10.1002/mds.28467
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2555: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 238: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Immune Mechanisms of Resistance to Cediranib in Ovarian Cancer.

Gopinathan, Ganga / Berlato, Chiara / Lakhani, Anissa / Szabova, Ludmila / Pegrum, Colin / Pedrosa, Ana-Rita / Laforets, Florian / Maniati, Eleni / Balkwill, Frances R

Molecular cancer therapeutics

2022 Volume 21, Issue 6, Page(s) 1030–1043

Abstract: This article investigates mechanisms of resistance to the VEGF receptor inhibitor cediranib in high-grade serous ovarian cancer (HGSOC), and defines rational combination therapies. We used three different syngeneic orthotopic mouse HGSOC models that ... ...

Abstract	This article investigates mechanisms of resistance to the VEGF receptor inhibitor cediranib in high-grade serous ovarian cancer (HGSOC), and defines rational combination therapies. We used three different syngeneic orthotopic mouse HGSOC models that replicated the human tumor microenvironment (TME). After 4 to 5 weeks treatment of established tumors, cediranib had antitumor activity with increased tumor T-cell infiltrates and alterations in myeloid cells. However, continued cediranib treatment did not change overall survival or the immune microenvironment in two of the three models. Moreover, treated mice developed additional peritoneal metastases not seen in controls. Cediranib-resistant tumors had intrinsically high levels of IL6 and JAK/STAT signaling and treatment increased endothelial STAT3 activation. Combination of cediranib with a murine anti-IL6 antibody was superior to monotherapy, increasing mouse survival, reducing blood vessel density, and pSTAT3, with increased T-cell infiltrates in both models. In a third HGSOC model, that had lower inherent IL6 JAK/STAT3 signaling in the TME but high programmed cell death protein 1 (PD-1) signaling, long-term cediranib treatment significantly increased overall survival. When the mice eventually relapsed, pSTAT3 was still reduced in the tumors but there were high levels of immune cell PD-1 and Programmed death-ligand 1. Combining cediranib with an anti-PD-1 antibody was superior to monotherapy in this model, increasing T cells and decreasing blood vessel densities. Bioinformatics analysis of two human HGSOC transcriptional datasets revealed distinct clusters of tumors with IL6 and PD-1 pathway expression patterns that replicated the mouse tumors. Combination of anti-IL6 or anti-PD-1 in these patients may increase activity of VEGFR inhibitors and prolong disease-free survival.
MeSH term(s)	Angiogenesis Inhibitors/pharmacology ; Animals ; Carcinoma, Ovarian Epithelial ; Cell Line, Tumor ; Female ; Humans ; Indoles ; Interleukin-6 ; Mice ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/genetics ; Ovarian Neoplasms/metabolism ; Programmed Cell Death 1 Receptor ; Quinazolines ; Tumor Microenvironment
Chemical Substances	Angiogenesis Inhibitors ; Indoles ; Interleukin-6 ; Programmed Cell Death 1 Receptor ; Quinazolines ; cediranib (NQU9IPY4K9)
Language	English
Publishing date	2022-03-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2063563-1
ISSN	1538-8514 ; 1535-7163
ISSN (online)	1538-8514
ISSN	1535-7163
DOI	10.1158/1535-7163.MCT-21-0689
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 5750: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: A novel cell line panel reveals non-genetic mediators of platinum resistance and phenotypic diversity in high grade serous ovarian cancer.

Hoare, J I / Hockings, H / Saxena, J / Silva, V L / Haughey, M J / Wood, G E / Nicolini, F / Mirza, H / McNeish, I A / Huang, W / Maniati, E / Graham, T A / Lockley, M

Gynecologic oncology

2022 Volume 167, Issue 1, Page(s) 96–106

Abstract: Objectives: Resistance to cancer therapy is an enduring challenge and accurate and reliable preclinical models are lacking. We interrogated this unmet need using high grade serous ovarian cancer (HGSC) as a disease model.: Methods: We created five in ...

Abstract	Objectives: Resistance to cancer therapy is an enduring challenge and accurate and reliable preclinical models are lacking. We interrogated this unmet need using high grade serous ovarian cancer (HGSC) as a disease model. Methods: We created five in vitro and two in vivo platinum-resistant HGSC models and characterised the entire cell panel via whole genome sequencing, RNASeq and creation of intraperitoneal models. Results: Mutational signature analysis indicated that platinum-resistant cell lines evolved from a pre-existing ancestral clone but a unifying mutational cause for drug resistance was not identified. However, cisplatin-resistant and carboplatin-resistant cells evolved recurrent changes in gene expression that significantly overlapped with independent samples obtained from multiple patients with relapsed HGSC. Gene Ontology Biological Pathways (GOBP) related to the tumour microenvironment, particularly the extracellular matrix, were repeatedly enriched in cisplatin-resistant cells, carboplatin-resistant cells and also in human resistant/refractory samples. The majority of significantly over-represented GOBP however, evolved uniquely in either cisplatin- or carboplatin-resistant cell lines resulting in diverse intraperitoneal behaviours that reflect different clinical manifestations of relapsed human HGSC. Conclusions: Our clinically relevant and usable models reveal a key role for non-genetic factors in the evolution of chemotherapy resistance. Biological pathways relevant to the extracellular matrix were repeatedly expressed by resistant cancer cells in multiple settings. This suggests that recurrent gene expression changes provide a fitness advantage during platinum therapy and also that cancer cell-intrinsic mechanisms influence the tumour microenvironment during the evolution of drug resistance. Candidate genes and pathways identified here could reveal therapeutic opportunities in platinum-resistant HGSC.
MeSH term(s)	Carboplatin/pharmacology ; Carboplatin/therapeutic use ; Carcinoma, Ovarian Epithelial ; Cell Line ; Cisplatin/pharmacology ; Cisplatin/therapeutic use ; Drug Resistance, Neoplasm/genetics ; Female ; Humans ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/genetics ; Ovarian Neoplasms/pathology ; Platinum/therapeutic use ; Tumor Microenvironment/genetics
Chemical Substances	Platinum (49DFR088MY) ; Carboplatin (BG3F62OND5) ; Cisplatin (Q20Q21Q62J)
Language	English
Publishing date	2022-07-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	801461-9
ISSN	1095-6859 ; 0090-8258
ISSN (online)	1095-6859
ISSN	0090-8258
DOI	10.1016/j.ygyno.2022.07.027
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 885: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: IL-17 mediates resistance to anti-VEGF therapy.

Maniati, Eleni / Hagemann, Thorsten

Nature medicine

2013 Volume 19, Issue 9, Page(s) 1092–1094

Abstract: Interleukin-17 (IL-17) released in the tumor microenvironment in response to drugs blocking vascular endothelial growth factor (VEGF) triggers stromal-derived inflammatory and VEGF-independent angiogenic programs that induce the drug refractoriness found ...

Abstract	Interleukin-17 (IL-17) released in the tumor microenvironment in response to drugs blocking vascular endothelial growth factor (VEGF) triggers stromal-derived inflammatory and VEGF-independent angiogenic programs that induce the drug refractoriness found in cancers resistant to anti-angiogenic therapy.
MeSH term(s)	Angiogenesis Inhibitors/pharmacology ; Animals ; Drug Resistance, Neoplasm ; Female ; Humans ; Interleukin-17/metabolism ; Male ; Neoplasms/drug therapy ; Neovascularization, Pathologic/immunology ; Th17 Cells/immunology ; Vascular Endothelial Growth Factor A/antagonists & inhibitors
Chemical Substances	Angiogenesis Inhibitors ; Interleukin-17 ; Vascular Endothelial Growth Factor A ; vascular endothelial growth factor A, mouse
Language	English
Publishing date	2013-09-07
Publishing country	United States
Document type	News ; Comment
ZDB-ID	1220066-9
ISSN	1546-170X ; 1078-8956
ISSN (online)	1546-170X
ISSN	1078-8956
DOI	10.1038/nm.3333
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4212: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 39: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Replication stress generates distinctive landscapes of DNA copy number alterations and chromosome scale losses.

Shaikh, Nadeem / Mazzagatti, Alice / De Angelis, Simone / Johnson, Sarah C / Bakker, Bjorn / Spierings, Diana C J / Wardenaar, René / Maniati, Eleni / Wang, Jun / Boemo, Michael A / Foijer, Floris / McClelland, Sarah E

Genome biology

2022 Volume 23, Issue 1, Page(s) 223

Abstract: Background: A major driver of cancer chromosomal instability is replication stress, the slowing or stalling of DNA replication. How replication stress and genomic instability are connected is not known. Aphidicolin-induced replication stress induces ... ...

Abstract	Background: A major driver of cancer chromosomal instability is replication stress, the slowing or stalling of DNA replication. How replication stress and genomic instability are connected is not known. Aphidicolin-induced replication stress induces breakages at common fragile sites, but the exact causes of fragility are debated, and acute genomic consequences of replication stress are not fully explored. Results: We characterize DNA copy number alterations (CNAs) in single, diploid non-transformed cells, caused by one cell cycle in the presence of either aphidicolin or hydroxyurea. Multiple types of CNAs are generated, associated with different genomic regions and features, and observed copy number landscapes are distinct between aphidicolin and hydroxyurea-induced replication stress. Coupling cell type-specific analysis of CNAs to gene expression and single-cell replication timing analyses pinpointed the causative large genes of the most recurrent chromosome-scale CNAs in aphidicolin. These are clustered on chromosome 7 in RPE1 epithelial cells but chromosome 1 in BJ fibroblasts. Chromosome arm level CNAs also generate acentric lagging chromatin and micronuclei containing these chromosomes. Conclusions: Chromosomal instability driven by replication stress occurs via focal CNAs and chromosome arm scale changes, with the latter confined to a very small subset of chromosome regions, potentially heavily skewing cancer genome evolution. Different inducers of replication stress lead to distinctive CNA landscapes providing the opportunity to derive copy number signatures of specific replication stress mechanisms. Single-cell CNA analysis thus reveals the impact of replication stress on the genome, providing insights into the molecular mechanisms which fuel chromosomal instability in cancer.
MeSH term(s)	Humans ; DNA Copy Number Variations ; Aphidicolin/pharmacology ; Hydroxyurea/pharmacology ; Neoplasms/genetics ; DNA ; Chromosomal Instability ; Chromosomes ; Chromatin
Chemical Substances	Aphidicolin (38966-21-1) ; Hydroxyurea (X6Q56QN5QC) ; DNA (9007-49-2) ; Chromatin
Language	English
Publishing date	2022-10-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2040529-7
ISSN	1474-760X ; 1474-760X
ISSN (online)	1474-760X
ISSN	1474-760X
DOI	10.1186/s13059-022-02781-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Extracellular matrix educates an immunoregulatory tumor macrophage phenotype found in ovarian cancer metastasis.

Puttock, E H / Tyler, E J / Manni, M / Maniati, E / Butterworth, C / Burger Ramos, M / Peerani, E / Hirani, P / Gauthier, V / Liu, Y / Maniscalco, G / Rajeeve, V / Cutillas, P / Trevisan, C / Pozzobon, M / Lockley, M / Rastrick, J / Läubli, H / White, A /

Pearce, O M T

Nature communications

2023 Volume 14, Issue 1, Page(s) 2514

Abstract: Recent studies have shown that the tumor extracellular matrix (ECM) associates with immunosuppression, and that targeting the ECM can improve immune infiltration and responsiveness to immunotherapy. A question that remains unresolved is whether the ECM ... ...

Abstract	Recent studies have shown that the tumor extracellular matrix (ECM) associates with immunosuppression, and that targeting the ECM can improve immune infiltration and responsiveness to immunotherapy. A question that remains unresolved is whether the ECM directly educates the immune phenotypes seen in tumors. Here, we identify a tumor-associated macrophage (TAM) population associated with poor prognosis, interruption of the cancer immunity cycle, and tumor ECM composition. To investigate whether the ECM was capable of generating this TAM phenotype, we developed a decellularized tissue model that retains the native ECM architecture and composition. Macrophages cultured on decellularized ovarian metastasis shared transcriptional profiles with the TAMs found in human tissue. ECM-educated macrophages have a tissue-remodeling and immunoregulatory phenotype, inducing altered T cell marker expression and proliferation. We conclude that the tumor ECM directly educates this macrophage population found in cancer tissues. Therefore, current and emerging cancer therapies that target the tumor ECM may be tailored to improve macrophage phenotype and their downstream regulation of immunity.
MeSH term(s)	Humans ; Female ; Macrophages/metabolism ; Extracellular Matrix/metabolism ; Ovarian Neoplasms/pathology ; Phenotype ; Tumor Microenvironment
Language	English
Publishing date	2023-05-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-38093-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Designing an Outer Membrane Protein (Omp-W) Based Vaccine for Immunization against

Tehrani, Sadra S / Jahangiri, Abolfazl / Taheri-Anganeh, Mortaza / Maghsoudi, Hossein / Khalili, Saeed / Fana, Saeed E / Maniati, Mahmood / Amani, Jafar

Recent patents on biotechnology

2020 Volume 14, Issue 4, Page(s) 312–324

Abstract: ... structure were predicted while overexpressed in E. coli.: Results: The designed vaccine is a stable ... overexpressed in an E. coli.: Conclusion: The multi-epitope vaccine is a suitable stimulator ...

Abstract	Background: Cholera triggered by Vibrio cholerae remains the main reason for morbidity and mortality all over the world. In addition, salmonellosis is regarded as an infectious disease that makes it essential for the identification and detection of Salmonella. With a beta-barrel structure consisting of eight non-parallel beta strands, OmpW family is widely distributed among gram-negative bacteria. Moreover, OmpW isolated from S. typhimurium and Vibrio cholerae can be used in vaccine design. Methods: Topology prediction was determined. T-cell and B-cell epitopes were selected from exposed areas, and sequence conservancy was evaluated. The remaining loops and inaccessible residues were removed to prepare OmpW-1. High antigenicity peptides were detected to replace inappropriate residues to obtain OmpW-2. Physicochemical properties were assessed, and antigenicity, hydrophobicity, flexibility, and accessibility were compared to the native Omp-W structure. Low score areas were removed from the designed structure for preparing the OmpW-3. To construct OmpW-4, TTFrC was used as T-CD4+ cell-stimulating factor and CTB as adjuvant to the end of the C-terminal of this sequence, which can increase the antigenicity and sequence density. The sequences were re-analyzed to delete the unfavorable residues. Besides, the solubility of the mature OmpW and the designed structure were predicted while overexpressed in E. coli. Results: The designed vaccine is a stable protein that has immune cells recognizing epitopes and is considered as an antigen. The construct can be overexpressed in an E. coli. Conclusion: The multi-epitope vaccine is a suitable stimulator for the immune system and would be a candidate for experimental research. Recent patents describe numerous inventions related to the clinical facets of vaccine peptide against human infectious disease.
MeSH term(s)	Antigens, Bacterial/chemistry ; Antigens, Bacterial/genetics ; Antigens, Bacterial/immunology ; Bacterial Outer Membrane Proteins/chemistry ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/immunology ; Bacterial Vaccines ; Cholera/microbiology ; Computational Biology ; Computer Simulation ; Epitopes/chemistry ; Epitopes/immunology ; Humans ; Patents as Topic ; Salmonella/chemistry ; Salmonella/immunology ; Salmonella Infections/microbiology ; Vaccines, Subunit ; Vibrio cholerae/chemistry ; Vibrio cholerae/immunology
Chemical Substances	Antigens, Bacterial ; Bacterial Outer Membrane Proteins ; Bacterial Vaccines ; Epitopes ; Vaccines, Subunit
Language	English
Publishing date	2020-09-28
Publishing country	United Arab Emirates
Document type	Journal Article
ISSN	2212-4012
ISSN (online)	2212-4012
DOI	10.2174/1874609813666200929113341
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Were Byzantine monks of the 13th-15th centuries holders of imperial grants?

Maniati-Kokkini Triantafyllitsa

Zbornik Radova Vizantološkog Instituta, Vol 2013, Iss 50-2, Pp 629-

2013 Volume 644

Abstract: A small number of imperial grants to monks appear in the Byzantine sources from the late 13th to the 15th centuries, and mainly in the 14th, before the Serbian expansion in Macedonia. Especially privileged with the right of bequeathal and dedication, ... ...

Abstract	A small number of imperial grants to monks appear in the Byzantine sources from the late 13th to the 15th centuries, and mainly in the 14th, before the Serbian expansion in Macedonia. Especially privileged with the right of bequeathal and dedication, they were given later to the monastery to which each of the monks belonged, mostly to the Serbian monastery of Chilandar in Athos. Due to characteristic differences they represent a special category between the pronoiai of laymen and the oikonomiai of monasteries.
Keywords	Athos ; basilikon ; Chilandar ; chrysobull ; eleemosyne ; gonikos-e ; horismos ; hyperpyron ; (imperial) grant ; kathegoumenos ; Menoikeion ; metochion ; monastery ; monk ; monydrion ; nomisma ; oikonomia ; oikonomos ; Palaiologoi ; Patmos ; paroikos ; posotes ; praktikon (of paradosis) ; pronoia ; pronoiar ; prostagma ; tax-exemption ; tax ; will ; History (General) and history of Europe ; D
Language	Bulgarian
Publishing date	2013-01-01T00:00:00Z
Publisher	Institute for Byzantine Studies of the Serbian Academy of Sciences and Arts
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED