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  1. Book: Cell-free gene expression

    Karim, Ashty S. / Jewett, Michael C.

    methods and protocols

    (Methods in molecular biology ; 2433 ; Springer protocols)

    2022  

    Author's details edited by Ashty S. Karim, Michael C. Jewett
    Series title Methods in molecular biology ; 2433
    Springer protocols
    Collection
    Keywords Gene expression ; Synthetic biology
    Subject code 572.865
    Language English
    Size xv, 435 Seiten, Illustrationen, Diagramme, 26 cm
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    Note Hier auch später erschienene, unveränderte Nachdrucke
    HBZ-ID HT021200535
    ISBN 978-1-0716-1997-1 ; 978-1-0716-2000-7 ; 9781071619988 ; 1-0716-1997-7 ; 1-0716-2000-2 ; 1071619985
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -2433- not available for loan Lesesaal EG

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  2. Article ; Online: A balancing act.

    Landwehr, Grant M / Jewett, Michael C

    Nature chemical biology

    2022  Volume 19, Issue 2, Page(s) 127–128

    Language English
    Publishing date 2022-12-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2202962-X
    ISSN 1552-4469 ; 1552-4450
    ISSN (online) 1552-4469
    ISSN 1552-4450
    DOI 10.1038/s41589-022-01183-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Zs.MG 90: Show issues

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  3. Article: Ribosome Pool Engineering Increases Protein Biosynthesis Yields.

    Kofman, Camila / Willi, Jessica A / Karim, Ashty S / Jewett, Michael C

    ACS central science

    2024  Volume 10, Issue 4, Page(s) 871–881

    Abstract: The biosynthetic capability of the bacterial ribosome motivates efforts to understand and harness sequence-optimized versions for synthetic biology. However, functional differences between natively occurring ribosomal RNA (rRNA) operon sequences remain ... ...

    Abstract The biosynthetic capability of the bacterial ribosome motivates efforts to understand and harness sequence-optimized versions for synthetic biology. However, functional differences between natively occurring ribosomal RNA (rRNA) operon sequences remain poorly characterized. Here, we use an
    Language English
    Publishing date 2024-03-20
    Publishing country United States
    Document type Journal Article
    ISSN 2374-7943
    ISSN 2374-7943
    DOI 10.1021/acscentsci.3c01413
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Progress in Engineering Synthetic Cells and Cell-Free Systems.

    Dogterom, Marileen / Kamat, Neha P / Jewett, Michael C / Adamala, Katarzyna P

    ACS synthetic biology

    2024  Volume 13, Issue 3, Page(s) 695–696

    MeSH term(s) Cell-Free System ; Artificial Cells ; Synthetic Biology ; Metabolic Engineering
    Language English
    Publishing date 2024-03-02
    Publishing country United States
    Document type Editorial
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.4c00100
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Mobile Translation Systems Generate Genomically Engineered

    Gowland, Samuel / Jewett, Michael C

    ACS synthetic biology

    2022  Volume 11, Issue 9, Page(s) 2969–2978

    Abstract: Cellular translation is responsible for the synthesis of proteins, a highly diverse class of macromolecules that form the basis of biological function. ... ...

    Abstract Cellular translation is responsible for the synthesis of proteins, a highly diverse class of macromolecules that form the basis of biological function. In
    MeSH term(s) Amino Acyl-tRNA Synthetases/genetics ; Amino Acyl-tRNA Synthetases/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Phenotype ; RNA, Transfer/genetics ; Recombinases/genetics ; Recombinases/metabolism
    Chemical Substances Recombinases ; RNA, Transfer (9014-25-9) ; Amino Acyl-tRNA Synthetases (EC 6.1.1.-)
    Language English
    Publishing date 2022-08-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.2c00099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Ribosomes read new languages.

    Jewett, Michael C / Hammerling, Michael J

    Nature chemical biology

    2020  Volume 16, Issue 5, Page(s) 486–488

    MeSH term(s) Codon ; Language ; Ribosomes
    Chemical Substances Codon
    Language English
    Publishing date 2020-04-06
    Publishing country United States
    Document type News ; Comment
    ZDB-ID 2202962-X
    ISSN 1552-4469 ; 1552-4450
    ISSN (online) 1552-4469
    ISSN 1552-4450
    DOI 10.1038/s41589-020-0522-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  7. Article ; Online: Using High-Throughput Experiments To Screen

    Lin, Liang / Kightlinger, Weston / Warfel, Katherine F / Jewett, Michael C / Mrksich, Milan

    ACS synthetic biology

    2024  Volume 13, Issue 4, Page(s) 1290–1302

    Abstract: The important roles that protein glycosylation plays in modulating the activities and efficacies of protein therapeutics have motivated the development of synthetic glycosylation systems in living bacteria and in vitro. A key challenge is the lack of ... ...

    Abstract The important roles that protein glycosylation plays in modulating the activities and efficacies of protein therapeutics have motivated the development of synthetic glycosylation systems in living bacteria and in vitro. A key challenge is the lack of glycosyltransferases that can efficiently and site-specifically glycosylate desired target proteins without the need to alter primary amino acid sequences at the acceptor site. Here, we report an efficient and systematic method to screen a library of glycosyltransferases capable of modifying comprehensive sets of acceptor peptide sequences in parallel. This approach is enabled by cell-free protein synthesis and mass spectrometry of self-assembled monolayers and is used to engineer a recently discovered prokaryotic
    MeSH term(s) Glycosylation ; Glycosyltransferases/metabolism ; Bacterial Proteins/metabolism ; Glucosyltransferases/metabolism ; Peptides/metabolism ; Bacteria/metabolism
    Chemical Substances N-glycosyltransferase, Nocardia aerocolonigenes (EC 2.4.1.-) ; Glycosyltransferases (EC 2.4.-) ; Bacterial Proteins ; Glucosyltransferases (EC 2.4.1.-) ; Peptides
    Language English
    Publishing date 2024-03-25
    Publishing country United States
    Document type Journal Article
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.3c00769
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs.

    Seki, Kosuke / Galindo, Joey L / Karim, Ashty S / Jewett, Michael C

    ACS chemical biology

    2023  Volume 18, Issue 6, Page(s) 1324–1334

    Abstract: Non-canonical amino acids (ncAAs) can be incorporated into peptides and proteins to create new properties and functions. Site-specific ncAA incorporation is typically enabled by orthogonal translation systems comprising a stop codon suppressing tRNA ( ... ...

    Abstract Non-canonical amino acids (ncAAs) can be incorporated into peptides and proteins to create new properties and functions. Site-specific ncAA incorporation is typically enabled by orthogonal translation systems comprising a stop codon suppressing tRNA (typically UAG), an aminoacyl-tRNA synthetase, and an ncAA of interest. Unfortunately, methods to discover and characterize suppressor tRNAs are limited because of laborious and time-consuming workflows in living cells. In this work, we develop an
    MeSH term(s) Codon, Terminator/genetics ; RNA, Transfer/chemistry ; Amino Acids/chemistry ; Amino Acyl-tRNA Synthetases/genetics ; Amino Acyl-tRNA Synthetases/metabolism ; Green Fluorescent Proteins/genetics ; Gene Expression
    Chemical Substances Codon, Terminator ; RNA, Transfer (9014-25-9) ; Amino Acids ; Amino Acyl-tRNA Synthetases (EC 6.1.1.-) ; Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2023-05-31
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.3c00051
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Cell Extracts from Bacteria and Yeast Retain Metabolic Activity after Extended Storage and Repeated Thawing.

    Rasor, Blake J / Karim, Ashty S / Alper, Hal S / Jewett, Michael C

    ACS synthetic biology

    2023  Volume 12, Issue 3, Page(s) 904–908

    Abstract: Cell-free synthetic biology enables rapid prototyping of biological parts and synthesis of proteins or metabolites in the absence of cell growth constraints. Cell-free systems are frequently made from crude cell extracts, where composition and activity ... ...

    Abstract Cell-free synthetic biology enables rapid prototyping of biological parts and synthesis of proteins or metabolites in the absence of cell growth constraints. Cell-free systems are frequently made from crude cell extracts, where composition and activity can vary significantly based on source strain, preparation and processing, reagents, and other considerations. This variability can cause extracts to be treated as black boxes for which empirical observations guide practical laboratory practices, including a hesitance to use dated or previously thawed extracts. To better understand the robustness of cell extracts over time, we assessed the activity of cell-free metabolism during storage. As a model, we studied conversion of glucose to 2,3-butanediol. We found that cell extracts from
    MeSH term(s) Saccharomyces cerevisiae/metabolism ; Cell Extracts ; Freezing ; Bacteria
    Chemical Substances Cell Extracts
    Language English
    Publishing date 2023-02-27
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.2c00685
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Lyophilization of premixed COVID-19 diagnostic RT-qPCR reactions enables stable long-term storage at elevated temperature.

    Hammerling, Michael J / Warfel, Katherine F / Jewett, Michael C

    Biotechnology journal

    2021  Volume 16, Issue 7, Page(s) e2000572

    Abstract: Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) diagnostic tests for SARS-CoV-2 are the cornerstone of the global testing infrastructure. However, these tests require cold-chain shipping to distribute, and the labor of skilled ... ...

    Abstract Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) diagnostic tests for SARS-CoV-2 are the cornerstone of the global testing infrastructure. However, these tests require cold-chain shipping to distribute, and the labor of skilled technicians to assemble reactions and interpret the results. Strategies to reduce shipping and labor costs at the point-of-care could aid in diagnostic testing scale-up and response to the COVID-19 outbreak, as well as in future outbreaks. In this study we test both lab-developed and commercial SARS-CoV-2 diagnostic RT-qPCR mixes for the ability to be stabilized against elevated temperature by lyophilization. Fully assembled reactions were lyophilized and stored for up to a month at ambient or elevated temperature and were subsequently assayed for their ability to detect dilutions of synthetic SARS-CoV-2 RNA. Of the mixes tested, we show that one commercial mix can maintain activity and sensitivity after storage for at least 30 days at ambient temperature after lyophilization. We also demonstrate that lyoprotectants such as disaccharides can stabilize freeze-dried diagnostic reactions against elevated temperatures (up to 50°C) for at least 30 days. We anticipate that the incorporation of these methods into SARS-CoV-2 diagnostic testing will improve testing pipelines by reducing labor at the testing facility and eliminating the need for cold-chain shipping.
    MeSH term(s) COVID-19 ; Freeze Drying ; Humans ; RNA, Viral/genetics ; SARS-CoV-2 ; Sensitivity and Specificity ; Temperature
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-06-10
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2221885-3
    ISSN 1860-7314 ; 1860-6768
    ISSN (online) 1860-7314
    ISSN 1860-6768
    DOI 10.1002/biot.202000572
    Database MEDical Literature Analysis and Retrieval System OnLINE

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