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  1. Article ; Online: Quantitative Structural Mapping of Insulin Vesicle Maturation in Beta Cells.

    Deshmukh, Aneesh / Loconte, Valentina / White, Kate L

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue 29 Suppl 1, Page(s) 1166

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.597
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  2. Article ; Online: The use of soft X-ray tomography to explore mitochondrial structure and function

    Valentina Loconte / Kate L. White

    Molecular Metabolism, Vol 57, Iss , Pp 101421- (2022)

    2022  

    Abstract: Background: Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three- ... ...

    Abstract Background: Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three-dimensional whole cell mapping technique is required. Scope of review: This review highlights the use of soft X-ray tomography (SXT) as a relatively high-throughput approach to quantify mitochondrial structure and function under multiple cellular conditions. Major conclusions: The use of SXT opens the door for mapping cellular rearrangements during critical processes such as insulin secretion, stem cell differentiation, or disease progression. SXT provides unique information such as biochemical compositions or molecular densities of organelles and allows for unbiased, label-free imaging of intact whole cells. Mapping mitochondria in the context of the near-native cellular environment will reveal more information regarding mitochondrial network functions within the cell.
    Keywords Structural biology ; Cell mapping ; Tomography ; Spatial biology ; Mitochondria ; Internal medicine ; RC31-1245
    Subject code 610
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: The use of soft X-ray tomography to explore mitochondrial structure and function.

    Loconte, Valentina / White, Kate L

    Molecular metabolism

    2021  Volume 57, Page(s) 101421

    Abstract: Background: Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three- ... ...

    Abstract Background: Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three-dimensional whole cell mapping technique is required.
    Scope of review: This review highlights the use of soft X-ray tomography (SXT) as a relatively high-throughput approach to quantify mitochondrial structure and function under multiple cellular conditions.
    Major conclusions: The use of SXT opens the door for mapping cellular rearrangements during critical processes such as insulin secretion, stem cell differentiation, or disease progression. SXT provides unique information such as biochemical compositions or molecular densities of organelles and allows for unbiased, label-free imaging of intact whole cells. Mapping mitochondria in the context of the near-native cellular environment will reveal more information regarding mitochondrial network functions within the cell.
    MeSH term(s) Mitochondria ; Tomography, X-Ray/methods
    Language English
    Publishing date 2021-12-20
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 2708735-9
    ISSN 2212-8778 ; 2212-8778
    ISSN (online) 2212-8778
    ISSN 2212-8778
    DOI 10.1016/j.molmet.2021.101421
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The Role of Soft X-ray Tomography in Generating Whole-cell Models.

    Loconte, Valentina / Chen, Jian-Hua / Vanslembrouck, Bieke / Ekman, Axel A / McDermott, Gerry / Gros, Mark A Le / Larabell, Carolyn A

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue Supplement_1, Page(s) 1170

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.600
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Soft X-ray 3D imaging: A Powerful Tool for Visualizing Virus Infections with Increased Resolution and Field of View.

    Chen, Jian-Hua / Vanslembrouck, Bieke / Ekman, Axel / Loconte, Valentina / Weinhardt, Venera / LeGros, Mark / Larabell, Carolyn

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue 29 Suppl 1, Page(s) 1179–1180

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.606
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Soft X-ray 3D Imaging: A Powerful Tool for Visualizing Virus Infections with Increased Resolution and Field of View.

    Chen, Jian-Hua / Vanslembrouck, Bieke / Ekman, Axel / Loconte, Valentina / Weinhardt, Venera / LeGros, Mark / Larabell, Carolyn

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue 29 Suppl 1, Page(s) 1155–1156

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.590
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Poliglusam Nanoparticles Activate T Cell Response in Breast Cancer Cell: an In Vivo and In Vitro Study.

    Soleimani, Neda / Vaseghi, Akbar / Loconte, Valentina

    Journal of fluorescence

    2019  Volume 29, Issue 4, Page(s) 1057–1064

    Abstract: Poliglusam nanoparticles are potential therapeutic agents for the treatment of cancer. In particular, their efficacy has been reported as delivery systems in breast cancer. The aim of this study is to propose a new immunotherapeutic strategy, using ... ...

    Abstract Poliglusam nanoparticles are potential therapeutic agents for the treatment of cancer. In particular, their efficacy has been reported as delivery systems in breast cancer. The aim of this study is to propose a new immunotherapeutic strategy, using Poliglusam nanoparticles as activators of the human immune response. Poliglusam nanoparticles were synthesized and characterized using both dynamic light scattering and electron microscopy. Whilst, their effectiveness in immune stimulation and detection of apoptosis was evaluated by cytokine and TUNEL assays. Finally, the cytokines pattern in splenocytes revealed an increase in IFN-γ production. The results of cytotoxicity on 4 T1 cells show an increase in the mortality rate with respect to the control cell line. The rate of apoptosis induced by Poliglusam nanoparticles on 4 T1 mouse breast cancer cell line is about 45% higher compared to MCF-7 human cells line, revealing the natural tendency of Poliglusam in increasing the production of IFN-γ in cancer cells. At the state-of-art of the knowledge, very few information have been achieved on the immunological effects of Poliglusam. This work is one of the first studies for the identification of non-functionalized Poliglusam nanoparticles impact on breast cancer. Thus, their immunotherapeutic effect, combined with an anticancer drug, can be employed as potential effective drug for eliminating breast cancer cells in the future.
    MeSH term(s) Animals ; Apoptosis/drug effects ; Breast Neoplasms/pathology ; Cell Transformation, Neoplastic ; Chitosan/chemistry ; Chitosan/pharmacology ; Cytokines/metabolism ; Female ; Humans ; Lymphocyte Activation/drug effects ; MCF-7 Cells ; Mice ; Mice, Inbred BALB C ; Nanoparticles ; Spleen/drug effects ; Spleen/metabolism ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology
    Chemical Substances Cytokines ; Chitosan (9012-76-4)
    Language English
    Publishing date 2019-09-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2016892-5
    ISSN 1573-4994 ; 1053-0509
    ISSN (online) 1573-4994
    ISSN 1053-0509
    DOI 10.1007/s10895-019-02423-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Nitrogen-fixing organelle in a marine alga.

    Coale, Tyler H / Loconte, Valentina / Turk-Kubo, Kendra A / Vanslembrouck, Bieke / Mak, Wing Kwan Esther / Cheung, Shunyan / Ekman, Axel / Chen, Jian-Hua / Hagino, Kyoko / Takano, Yoshihito / Nishimura, Tomohiro / Adachi, Masao / Le Gros, Mark / Larabell, Carolyn / Zehr, Jonathan P

    Science (New York, N.Y.)

    2024  Volume 384, Issue 6692, Page(s) 217–222

    Abstract: Symbiotic interactions were key to the evolution of chloroplast and mitochondria organelles, which mediate carbon and energy metabolism in eukaryotes. Biological nitrogen fixation, the reduction of abundant atmospheric nitrogen gas ( ... ...

    Abstract Symbiotic interactions were key to the evolution of chloroplast and mitochondria organelles, which mediate carbon and energy metabolism in eukaryotes. Biological nitrogen fixation, the reduction of abundant atmospheric nitrogen gas (N
    MeSH term(s) Cyanobacteria/genetics ; Cyanobacteria/metabolism ; Haptophyta/microbiology ; Nitrogen/metabolism ; Nitrogen Fixation/genetics ; Seawater/microbiology ; Symbiosis ; Mitochondria/metabolism ; Chloroplasts/metabolism
    Chemical Substances Nitrogen (N762921K75)
    Language English
    Publishing date 2024-04-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.adk1075
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 27: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 77: Show issues

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  9. Article ; Online: Soft X-ray Tomography for Mapping and Quantifying Intracellular Organelle Interactions.

    Loconte, Valentina / Singla, Jitin / Li, Angdi / Chen, Jian-Hua / Ekman, Axel / McDermott, Gerry / Sali, Andrej / Gros, Mark Le / White, Kate L / Larabell, Carolyn A

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue Supplement_1, Page(s) 1181

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.607
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Soft X-ray tomograms provide a structural basis for whole-cell modeling.

    Loconte, Valentina / Chen, Jian-Hua / Vanslembrouck, Bieke / Ekman, Axel A / McDermott, Gerry / Le Gros, Mark A / Larabell, Carolyn A

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    2022  Volume 37, Issue 1, Page(s) e22681

    Abstract: Developing in silico models that accurately reflect a whole, functional cell is an ongoing challenge in biology. Current efforts bring together mathematical models, probabilistic models, visual representations, and data to create a multi-scale ... ...

    Abstract Developing in silico models that accurately reflect a whole, functional cell is an ongoing challenge in biology. Current efforts bring together mathematical models, probabilistic models, visual representations, and data to create a multi-scale description of cellular processes. A realistic whole-cell model requires imaging data since it provides spatial constraints and other critical cellular characteristics that are still impossible to obtain by calculation alone. This review introduces Soft X-ray Tomography (SXT) as a powerful imaging technique to visualize and quantify the mesoscopic (~25 nm spatial scale) organelle landscape in whole cells. SXT generates three-dimensional reconstructions of cellular ultrastructure and provides a measured structural framework for whole-cell modeling. Combining SXT with data from disparate technologies at varying spatial resolutions provides further biochemical details and constraints for modeling cellular mechanisms. We conclude, based on the results discussed here, that SXT provides a foundational dataset for a broad spectrum of whole-cell modeling experiments.
    MeSH term(s) X-Rays ; Imaging, Three-Dimensional/methods ; Tomography, X-Ray/methods ; Organelles
    Language English
    Publishing date 2022-05-20
    Publishing country United States
    Document type Review ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 639186-2
    ISSN 1530-6860 ; 0892-6638
    ISSN (online) 1530-6860
    ISSN 0892-6638
    DOI 10.1096/fj.202200253R
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2266: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 296: Show issues

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