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Article ; Online: Isolation, purification, and structural characterization of polysaccharides from Codonopsis pilosula and its therapeutic effects on non-alcoholic fatty liver disease in vitro and in vivo.

Ma, Kai / Yi, Xin / Yang, Shu-Ting / Zhu, Hua / Liu, Tian-Yu / Jia, Si-Si / Fan, Jia-Hao / Hu, De-Jun / Lv, Guang-Ping / Huang, He

International journal of biological macromolecules

2024 Volume 265, Issue Pt 2, Page(s) 130988

Abstract: Codonopsis pilosula is a famous edible and medicinal plants, in which polysaccharides are recognized as one of the important active ingredients. A neutral polysaccharide (CPP-1) was purified from C. pilosula. The structure was characterized by HPSEC- ... ...

Abstract	Codonopsis pilosula is a famous edible and medicinal plants, in which polysaccharides are recognized as one of the important active ingredients. A neutral polysaccharide (CPP-1) was purified from C. pilosula. The structure was characterized by HPSEC-MALLS-RID, UV, FT-IR, GC-MS, methylation analysis, and NMR. The results showed that CPP-1 was a homogeneous pure polysaccharide, mainly containing fructose and glucose, and a small amount of arabinose. Methylation analysis showed that CPP-1 composed of →1)-Fruf-(2→, Fruf-(1→ and Glcp-(1→ residues. Combined the NMR results the structure of CPP-1 was confirmed as α-D-Glcp-(1 → [2)-β-D-Fruf-(1 → 2)-β-D-Fruf-(1]
MeSH term(s)	Animals ; Mice ; Non-alcoholic Fatty Liver Disease/drug therapy ; Codonopsis/chemistry ; Spectroscopy, Fourier Transform Infrared ; Polysaccharides/pharmacology ; Polysaccharides/therapeutic use ; Polysaccharides/chemistry ; Antioxidants/pharmacology
Chemical Substances	Polysaccharides ; Antioxidants
Language	English
Publishing date	2024-03-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.130988
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Article ; Online: Traditional Chinese Medicine and Sarcopenia

Chao-yang Guo / Yun-jing Ma / Shu-ting Liu / Ran-ran Zhu / Xiao-ting Xu / Zhen-rui Li / Lei Fang

Frontiers in Aging Neuroscience, Vol

A Systematic Review

2022 Volume 14

Abstract: Sarcopenia has become a key challenge for healthy aging in older adults. However, it remains unclear whether traditional Chinese medicine can effectively treat sarcopenia. This systematic review analyzes the current evidence for the effect of traditional ...

Abstract	Sarcopenia has become a key challenge for healthy aging in older adults. However, it remains unclear whether traditional Chinese medicine can effectively treat sarcopenia. This systematic review analyzes the current evidence for the effect of traditional Chinese medicine (TCM) on sarcopenia. We searched for articles regarding sarcopenia treated by TCM in Cochrane library, PubMed, SinoMed, Web of Science, Embase, and the China National Knowledge Infrastructure (from inception until 10 December 2021). Two researchers independently screened the literature in accordance with the inclusion and exclusion criteria designed by PICOS principles. The risk of bias was assessed by the Cochrane Risk of Bias (ROB) tool. The quality of evidence was assessed by the grading of recommendations, assessment, development, and evaluation (GRADE). Participants’ characteristics, interventions, and the relevant results of the included studies were extracted and synthesized in a narrative way. The total number of participants in the 21 included studies was 1,330. Most of the studies evaluated physical function (n = 20) and muscle strength (n = 18), and a small number of studies (n = 6) assessed muscle mass. Overall, it was found that TCM had a positive impact on muscle strength (grip strength, chair stand test) and physical function (6-m walking speed, timed up and go test, sit and reach) in patients with sarcopenia, inconsistent evidence of effects on muscle mass. However, the small sample size of the included studies led to imprecision in the results, and the presence of blinding of the studies, allocation concealment, and unreasonable problems with the control group design made the results low grade. Among these results, the quality of evidence for grip strength (n = 10) was of medium grade, and the quality of evidence related to the remaining indicators was of low grade. This systematic review showed that traditional Chinese Qigong exercises and Chinese herbal medicine have a positive and important effect on physical performance and ...
Keywords	sarcopenia ; traditional Chinese medicine ; Chinese herb ; Qigong exercise ; acupuncture ; systematic review ; Neurosciences. Biological psychiatry. Neuropsychiatry ; RC321-571
Subject code	796
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Traditional Chinese Medicine and Sarcopenia: A Systematic Review.

Guo, Chao-Yang / Ma, Yun-Jing / Liu, Shu-Ting / Zhu, Ran-Ran / Xu, Xiao-Ting / Li, Zhen-Rui / Fang, Lei

Frontiers in aging neuroscience

2022 Volume 14, Page(s) 872233

Abstract	Sarcopenia has become a key challenge for healthy aging in older adults. However, it remains unclear whether traditional Chinese medicine can effectively treat sarcopenia. This systematic review analyzes the current evidence for the effect of traditional Chinese medicine (TCM) on sarcopenia. We searched for articles regarding sarcopenia treated by TCM in Cochrane library, PubMed, SinoMed, Web of Science, Embase, and the China National Knowledge Infrastructure (from inception until 10 December 2021). Two researchers independently screened the literature in accordance with the inclusion and exclusion criteria designed by PICOS principles. The risk of bias was assessed by the Cochrane Risk of Bias (ROB) tool. The quality of evidence was assessed by the grading of recommendations, assessment, development, and evaluation (GRADE). Participants' characteristics, interventions, and the relevant results of the included studies were extracted and synthesized in a narrative way. The total number of participants in the 21 included studies was 1,330. Most of the studies evaluated physical function (
Language	English
Publishing date	2022-05-13
Publishing country	Switzerland
Document type	Systematic Review
ZDB-ID	2558898-9
ISSN	1663-4365
ISSN	1663-4365
DOI	10.3389/fnagi.2022.872233
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Article ; Online: Sensitivity and reusability of a simple microbial fuel cell-based sensor for detecting bisphenol A in wastewater.

Zhu, Ting-Jun / Lin, Chi-Wen / Liu, Shu-Hui

Chemosphere

2023 Volume 320, Page(s) 138082

Abstract: Polycarbonate plastic processing wastewater contains high concentrations of bisphenol A (BPA), requiring a real-time technology to monitor wastewater containing BPA. Since the activity of electrogenic microorganisms on the anode surface of the microbial ... ...

Abstract	Polycarbonate plastic processing wastewater contains high concentrations of bisphenol A (BPA), requiring a real-time technology to monitor wastewater containing BPA. Since the activity of electrogenic microorganisms on the anode surface of the microbial fuel cell (MFC) sensor is inhibited by exposure to contaminants, the toxicity of contaminants in wastewater can be determined by observing the variation in voltage output from the MFC sensor. The simple MFC sensor that is developed in this work exhibited a significant decrease in voltage output in BPA-containing wastewater concentration of 5-100 mg/L. Sensitivity analysis revealed that the voltage change (ΔV) was strongly correlated with the BPA concentration, with R
MeSH term(s)	Bioelectric Energy Sources ; Wastewater ; Benzhydryl Compounds/analysis ; Phenols/analysis ; Electrodes ; Electricity
Chemical Substances	Wastewater ; bisphenol A (MLT3645I99) ; Benzhydryl Compounds ; Phenols
Language	English
Publishing date	2023-02-08
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2023.138082
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Article ; Online: Gender differences in family meal frequency and their association with substance use and mental health among middle and high school students

Ting Luo / Sharon E. Cummins / Shu-Hong Zhu

Frontiers in Public Health, Vol

2023 Volume 11

Abstract: BackgroundFamily meals are associated with adolescent health outcomes. Studies have reported that girls are less likely than boys to have dinner with their families.PurposeThis study examined gender differences in family meal frequency and the ... ...

Abstract	BackgroundFamily meals are associated with adolescent health outcomes. Studies have reported that girls are less likely than boys to have dinner with their families.PurposeThis study examined gender differences in family meal frequency and the relationship between meal frequency and other health measures, using a large and representative sample of California middle and high school students.MethodsThis study analyzed data from the 2019–2020 California Student Tobacco Survey (159,904 students in grades 8, 10, and 12). Dinner with the family 5–7 times per week was defined as high frequency. Students reported substance use (of tobacco, marijuana, and alcohol) and rated their mental health and happiness in their home life. All analyses were weighted to reflect the California student population.ResultsFewer than half (44.7%) of students reported a high frequency of family meals, with boys more likely than girls and those who identified their gender in another way the least likely to do so (48.3%, 42.2%, 34.0%, respectively). Gender differences persisted across demographics and the quality of family relationships, and were evident as early as eighth grade. Less frequent family meals were associated with poorer mental health (OR = 1.34, 95% CI: 1.29–1.40) and substance use (OR = 1.27, 95% CI: 1.21–1.32), controlling for the effects of demographics and family dynamics.ConclusionGender differences in family meal frequency emerge early in adolescence and persist across demographics and family relationships. Given that family meals play a protective role in an adolescent’s life, these gender differences are concerning.
Keywords	family meal frequency ; gender differences ; family dynamics ; substance use ; mental health ; Public aspects of medicine ; RA1-1270
Subject code	796
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Oral administration of octacosanol modulates the gut bacteria and protects the intestinal barrier in ulcerative colitis mice

Miao, Shu‐Ting / Lu, Qian‐Shan / Zhou, Ying‐Jun / Chang, Ya‐Ning / Xu, Tao / Zhu, Meng‐Yu

Journal of Food Biochemistry. 2022 Oct., v. 46, no. 10 p.e14284-

2022

Abstract: Octacosanol (Oct), a kind of long‐chain fatty alcohol extracted from rice bran was applied to study its effects on alleviating ulcerative colitis (UC). Oct was orally administered at 10 mg/kg (Oct‐L) and 30 mg/kg (Oct‐H) to dextran sulfate sodium (DSS)‐ ... ...

Abstract	Octacosanol (Oct), a kind of long‐chain fatty alcohol extracted from rice bran was applied to study its effects on alleviating ulcerative colitis (UC). Oct was orally administered at 10 mg/kg (Oct‐L) and 30 mg/kg (Oct‐H) to dextran sulfate sodium (DSS)‐induced mice. Here, we reported that oral administration of 30 mg/kg Oct can significantly prevent the weight loss, colon shortening, and decrease the disease activity index (DAI) score. Oct‐H supplementation modified the intestinal flora by lowering the Firmicutes/Bacteroidetes (F/B) ratio, increasing the abundance of Prevotellaceae, S24‐7, Turicibacter, and meanwhile decreasing Enterococcus and Stenotrophomonas. Based on the PICRUSt2 analysis, Oct‐H may exert effects by anti‐inflammation and xenobiotics degradation. Furthermore, short‐chain fatty acids (SCFAs) levels were raised and the integrity of the gut barrier was protected. In conclusion, Oct‐H can relieve clinical symptoms, modulate the gut bacteria and protect the intestinal barrier in UC mice, suggesting the potential of Oct as a food supplementation in alleviating UC. PRACTICAL APPLICATIONS: Ulcerative colitis (UC) is a hard‐to‐cure disease, with increasing morbidity in recent years. Therefore, finding out a food supplement to alleviate UC is very meaningful. In this work, we showed that octacosanol significantly alleviated ulcerative colitis in mice. We revealed, for the first time, octacosanol's effects on protecting the integrity of the gut barrier, modulating the intestinal flora and its metabolism (SCFAs). Therefore, octacosanol was expected to prevent colitis in an all‐round way. Our research might also lay the theoretical foundation for the further development of related functional foods.
Keywords	Bacteroidetes ; Enterococcus ; Stenotrophomonas ; absorption barrier ; colon ; dextran sulfate ; dietary supplements ; fatty alcohols ; intestinal microorganisms ; metabolism ; morbidity ; oral administration ; rice bran ; ulcerative colitis ; weight loss ; xenobiotics
Language	English
Dates of publication	2022-10
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	433846-7
ISSN	1745-4514 ; 0145-8884
ISSN (online)	1745-4514
ISSN	0145-8884
DOI	10.1111/jfbc.14284
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Gender differences in family meal frequency and their association with substance use and mental health among middle and high school students.

Luo, Ting / Cummins, Sharon E / Zhu, Shu-Hong

Frontiers in public health

2023 Volume 11, Page(s) 1123396

Abstract: Background: Family meals are associated with adolescent health outcomes. Studies have reported that girls are less likely than boys to have dinner with their families.: Purpose: This study examined gender differences in family meal frequency and the ... ...

Abstract	Background: Family meals are associated with adolescent health outcomes. Studies have reported that girls are less likely than boys to have dinner with their families. Purpose: This study examined gender differences in family meal frequency and the relationship between meal frequency and other health measures, using a large and representative sample of California middle and high school students. Methods: This study analyzed data from the 2019-2020 California Student Tobacco Survey (159,904 students in grades 8, 10, and 12). Dinner with the family 5-7 times per week was defined as high frequency. Students reported substance use (of tobacco, marijuana, and alcohol) and rated their mental health and happiness in their home life. All analyses were weighted to reflect the California student population. Results: Fewer than half (44.7%) of students reported a high frequency of family meals, with boys more likely than girls and those who identified their gender in another way the least likely to do so (48.3%, 42.2%, 34.0%, respectively). Gender differences persisted across demographics and the quality of family relationships, and were evident as early as eighth grade. Less frequent family meals were associated with poorer mental health (OR = 1.34, 95% CI: 1.29-1.40) and substance use (OR = 1.27, 95% CI: 1.21-1.32), controlling for the effects of demographics and family dynamics. Conclusion: Gender differences in family meal frequency emerge early in adolescence and persist across demographics and family relationships. Given that family meals play a protective role in an adolescent's life, these gender differences are concerning.
MeSH term(s)	Male ; Female ; Adolescent ; Humans ; Mental Health ; Sex Factors ; Meals ; Substance-Related Disorders/epidemiology ; Students/psychology
Language	English
Publishing date	2023-04-14
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2023.1123396
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Article: Analysis of Influencing Factors of Family Resilience in Children with Congenital Tibial Pseudoarthrosis.

Xie, Jian-Hui / Mei, Hai-Bo / Ouyang, Ya-Qi / Huang, Yuan / Liu, Kun / Zhu, Guang-Hui / Hou, Shu-Ting / Wang, Lan-Ying

Risk management and healthcare policy

2022 Volume 15, Page(s) 331–337

Abstract: Aim: To investigate the status of the family resilience score of caregivers of children with congenital pseudarthrosis of the tibia (CPT) and analyse its influencing factors to provide a reference and basis for clinical formulation of a family ... ...

Abstract	Aim: To investigate the status of the family resilience score of caregivers of children with congenital pseudarthrosis of the tibia (CPT) and analyse its influencing factors to provide a reference and basis for clinical formulation of a family resilience intervention programme. Methods: A total of 379 families of children with CPT were included in this study, which used a combination questionnaire to investigate the families of children with CPT. The researchers sent a link to the questionnaire to all eligible subjects to conduct a survey on the characteristics of families of children with CPT. Single factor analysis was used to compare the total scores of family resilience. Multiple linear regression was used to determine meaningful variables for single factor analysis. Results: The average family resilience score was 151.79 ± 30.79, the lowest score was 20 and the highest score was 231. The CPT usually occurred in unilateral limbs (37.63%). The payment methods of medical expenses were through a rural cooperative medical system (44.85%). The caregivers were mostly the mothers (62.80%) and caregivers were 31-40 years old (62.27%). Most caregivers were farmers (30.34%). The average monthly income of the families in the past year was 1000-5000 yuan (54.09%). There were significant differences in family resilience scores between caregivers' working conditions ( Conclusion: The caregivers' working conditions and the average monthly income of families were the influencing factors in the family resilience score.
Language	English
Publishing date	2022-02-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2495128-6
ISSN	1179-1594
ISSN	1179-1594
DOI	10.2147/RMHP.S339503
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Article ; Online: VFG-Chip

Shu-Ting Xie / Long-Jun Ding / Fu-Yi Huang / Yi Zhao / Xin-Li An / Jian-Qiang Su / Guo-Xin Sun / Ya-Qiong Song / Yong-Guan Zhu

Environment International, Vol 172, Iss , Pp 107761- (2023)

A high-throughput qPCR microarray for profiling virulence factor genes from the environment

2023

Abstract: As zoonotic pathogens are threatening public health globally, the virulence factor genes (VFGs) they carry underlie latent risk in the environment. However, profiling VFGs in the environment is still in its infancy due to lack of efficient and reliable ... ...

Abstract	As zoonotic pathogens are threatening public health globally, the virulence factor genes (VFGs) they carry underlie latent risk in the environment. However, profiling VFGs in the environment is still in its infancy due to lack of efficient and reliable quantification tools. Here, we developed a novel high-throughput qPCR (HT-qPCR) chip, termed as VFG-Chip, to comprehensively quantify the abundances of targeted VFGs in the environment. A total of 96 VFGs from four bacterial pathogens including Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, and Salmonella enterica were targeted by 120 primer pairs, which were involved in encoding five types of virulence factors (VFs) like toxin, adherence, secretion system, immune evasion/invasion, and iron uptake. The specificity of VFG-Chip was both verified computationally and experimentally, with high identity of amplicon sequencing and melting curves analysis proving its robust capability. The VFG-Chip also displayed high sensitivity (by plasmid serial dilution test) and amplification efficiency averaging 97.7%. We successfully applied the VFG-Chip to profile the distribution of VFGs along a wastewater treatment system with 69 VFGs detected in total. Overall, the VFG-Chip provides a robust tool for comprehensively quantifying VFGs in the environment, and thus provides novel information in assessing the health risks of zoonotic pathogens in the environment.
Keywords	Virulence factor genes ; High-throughput qPCR ; VFG-Chip ; Zoonotic pathogenic bacteria ; Wastewater treatment system ; Environmental sciences ; GE1-350
Subject code	600
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Azadirachtin Inhibits Nuclear Receptor

Fan, Shu-Ting / Wu, Mian-Zhi / Liu, Chang / Li, Hua-Hong / Huang, Shang-Huan / Zheng, Zi-Jing / Ye, Xi-Yu / Tan, Jin-Fang / Zhu, Guan-Heng

Journal of agricultural and food chemistry

2023 Volume 71, Issue 42, Page(s) 15497–15505

Abstract: Azadirachtin has been used to control agricultural pests for a long time; however, the molecular mechanism of azadirachtin on lepidopterans is still not clear. In this study, the fourth instar larvae of fall armyworm were fed with azadirachtin, and then ... ...

Abstract	Azadirachtin has been used to control agricultural pests for a long time; however, the molecular mechanism of azadirachtin on lepidopterans is still not clear. In this study, the fourth instar larvae of fall armyworm were fed with azadirachtin, and then the ecdysis was blocked in the fourth instar larval stage (L4). The prothoracic glands (PGs) of the treated larvae were dissected for RNA sequencing to determine the effect of azadirachtin on ecdysis inhibition. Interestingly, one of the PG-enriched genes, the nuclear hormone receptor 3 (
MeSH term(s)	Animals ; Molting/genetics ; Larva/metabolism ; Spodoptera/genetics ; Receptors, Cytoplasmic and Nuclear/genetics ; Receptors, Cytoplasmic and Nuclear/metabolism
Chemical Substances	azadirachtin (O4U1SAF85H) ; Receptors, Cytoplasmic and Nuclear
Language	English
Publishing date	2023-10-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c05508
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