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Article ; Online: The Influence of the Relative Timing between Pole and Heel Strike on Lower Limb Loading among Young and Older Naïve Pole Walkers.

Di Bacco, Vincenzo E / Kiriella, Jeevaka B / Gage, William H

Translational sports medicine

2022 Volume 2022, Page(s) 3938075

Abstract: ... walkers. Fourteen young (4 F; 25.3 ± 5.4 years) and 8 older adults (4 F; 68.5 ± 3.2 years) performed PW ...

Abstract	Current research is unclear with respect to whether pole walking (PW) reduces lower limb loading when compared to regular walking (RW). Contradictory findings in the literature may be related to the relative timing between pole and foot contact events, which were examined in the current study among naïve pole walkers. Fourteen young (4 F; 25.3 ± 5.4 years) and 8 older adults (4 F; 68.5 ± 3.2 years) performed PW and RW trials along a force plate embedded walkway at two different visits. The time difference between pole and foot contact during both the onset of ground contact and the peak force application was calculated. Several kinetic measures were calculated for the lower limbs and poles. A significant decrease during PW, compared to RW, was found for foot impulse (2.1%;
Language	English
Publishing date	2022-07-07
Publishing country	England
Document type	Journal Article
ISSN	2573-8488
ISSN (online)	2573-8488
DOI	10.1155/2022/3938075
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Epitope-targeting platform for broadly protective influenza vaccines.

Zeigler, David F / Gage, Emily / Clegg, Christopher H

PloS one

2021 Volume 16, Issue 5, Page(s) e0252170

Abstract: ... against 5LD50 influenza B and H1N1 challenge; combining stem and M2e antigens increased survival to 90 ... in type B viruses. One new target in the HA stem stimulated 100% survival, highlighting the value ... of this simple epitope discovery strategy. A candidate influenza B vaccine targeting two adjacent HA stem sites ...

Abstract	Seasonal influenza vaccines are often ineffective because they elicit strain-specific antibody responses to mutation-prone sites on the hemagglutinin (HA) head. Vaccines that provide long-lasting immunity to conserved epitopes are needed. Recently, we reported a nanoparticle-based vaccine platform produced by solid-phase peptide synthesis (SPPS) for targeting linear and helical protein-based epitopes. Here, we illustrate its potential for building broadly protective influenza vaccines. Targeting known epitopes in the HA stem, neuraminidase (NA) active site, and M2 ectodomain (M2e) conferred 50-75% survival against 5LD50 influenza B and H1N1 challenge; combining stem and M2e antigens increased survival to 90%. Additionally, protein sequence and structural information were employed in tandem to identify alternative epitopes that stimulate greater protection; we report three novel HA and NA sites that are highly conserved in type B viruses. One new target in the HA stem stimulated 100% survival, highlighting the value of this simple epitope discovery strategy. A candidate influenza B vaccine targeting two adjacent HA stem sites led to >104-fold reduction in pulmonary viral load. These studies describe a compelling platform for building vaccines that target conserved influenza epitopes.
MeSH term(s)	Animals ; Antibodies, Viral/immunology ; Epitopes/immunology ; Hemagglutinin Glycoproteins, Influenza Virus/immunology ; Humans ; Influenza B virus/immunology ; Influenza, Human/immunology ; Influenza, Human/prevention & control ; Influenza, Human/virology ; Mice ; Orthomyxoviridae Infections/immunology ; Orthomyxoviridae Infections/virology
Chemical Substances	Antibodies, Viral ; Epitopes ; Hemagglutinin Glycoproteins, Influenza Virus
Language	English
Publishing date	2021-05-27
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S.
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0252170
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Article ; Online: Novel chlorine-extending polymer coating with prolonged antiviral activity against SARS-CoV-2.

Zhang, Y / Choi, Y W / Demir, B / Ekbataniamiri, F / Fulton, M L / Ma, M / Schang, L M / Purevdorj-Gage, L / Qiao, M

Letters in applied microbiology

2022 Volume 75, Issue 5, Page(s) 1346–1353

Abstract: We previously reported a novel polymeric surface coating, namely, HaloFilm™ that can immobilize and extend the antimicrobial activity of chlorine on surfaces. In this study, we demonstrated the continuous antiviral efficacy of HaloFilm when applied on ... ...

Abstract	We previously reported a novel polymeric surface coating, namely, HaloFilm™ that can immobilize and extend the antimicrobial activity of chlorine on surfaces. In this study, we demonstrated the continuous antiviral efficacy of HaloFilm when applied on stainless steel and cotton gauze as two representative models for non-porous and porous surfaces against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Forty-eight hours post HaloFilm application and chlorination and 2 h post the viral challenge, the inoculum titre was reduced by 2.25 ± 0.33 and ≥4.36 ± 0.23 log
MeSH term(s)	Humans ; SARS-CoV-2 ; Antiviral Agents/pharmacology ; Chlorine/pharmacology ; Polymers/pharmacology ; Stainless Steel ; Copper ; COVID-19
Chemical Substances	Antiviral Agents ; Chlorine (4R7X1O2820) ; Polymers ; Stainless Steel (12597-68-1) ; Copper (789U1901C5)
Language	English
Publishing date	2022-08-31
Publishing country	England
Document type	Journal Article
ZDB-ID	632584-1
ISSN	1472-765X ; 0266-8254
ISSN (online)	1472-765X
ISSN	0266-8254
DOI	10.1111/lam.13804
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Article ; Online: Epitope-targeting platform for broadly protective influenza vaccines.

David F Zeigler / Emily Gage / Christopher H Clegg

PLoS ONE, Vol 16, Iss 5, p e

2021 Volume 0252170

Abstract	Seasonal influenza vaccines are often ineffective because they elicit strain-specific antibody responses to mutation-prone sites on the hemagglutinin (HA) head. Vaccines that provide long-lasting immunity to conserved epitopes are needed. Recently, we reported a nanoparticle-based vaccine platform produced by solid-phase peptide synthesis (SPPS) for targeting linear and helical protein-based epitopes. Here, we illustrate its potential for building broadly protective influenza vaccines. Targeting known epitopes in the HA stem, neuraminidase (NA) active site, and M2 ectodomain (M2e) conferred 50-75% survival against 5LD50 influenza B and H1N1 challenge; combining stem and M2e antigens increased survival to 90%. Additionally, protein sequence and structural information were employed in tandem to identify alternative epitopes that stimulate greater protection; we report three novel HA and NA sites that are highly conserved in type B viruses. One new target in the HA stem stimulated 100% survival, highlighting the value of this simple epitope discovery strategy. A candidate influenza B vaccine targeting two adjacent HA stem sites led to >104-fold reduction in pulmonary viral load. These studies describe a compelling platform for building vaccines that target conserved influenza epitopes.
Keywords	Medicine ; R ; Science ; Q
Subject code	571
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: BECC438b TLR4 agonist supports unique immune response profiles from nasal and muscular DTaP pertussis vaccines in murine challenge models.

DeJong, Megan A / Wolf, M Allison / Bitzer, Graham J / Hall, Jesse M / Fitzgerald, Nicholas A / Pyles, Gage M / Huckaby, Annalisa B / Petty, Jonathan E / Lee, Katherine / Barbier, Mariette / Bevere, Justin R / Ernst, Robert K / Damron, F Heath

Infection and immunity

2024 Volume 92, Issue 3, Page(s) e0022323

Abstract: The protection afforded by acellular pertussis vaccines wanes over time, and there is a need to develop improved vaccine formulations. Options to improve the vaccines involve the utilization of different adjuvants and administration via different routes. ...

Abstract	The protection afforded by acellular pertussis vaccines wanes over time, and there is a need to develop improved vaccine formulations. Options to improve the vaccines involve the utilization of different adjuvants and administration via different routes. While intramuscular (IM) vaccination provides a robust systemic immune response, intranasal (IN) vaccination theoretically induces a localized immune response within the nasal cavity. In the case of a
MeSH term(s)	Animals ; Mice ; Whooping Cough/prevention & control ; Diphtheria-Tetanus-acellular Pertussis Vaccines ; Toll-Like Receptor 4 ; Pertussis Vaccine ; Diphtheria-Tetanus-Pertussis Vaccine ; Bordetella pertussis ; Adjuvants, Immunologic ; Immunity ; Antibodies, Bacterial
Chemical Substances	Diphtheria-Tetanus-acellular Pertussis Vaccines ; Toll-Like Receptor 4 ; Pertussis Vaccine ; Diphtheria-Tetanus-Pertussis Vaccine ; Adjuvants, Immunologic ; Antibodies, Bacterial
Language	English
Publishing date	2024-02-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	218698-6
ISSN	1098-5522 ; 0019-9567
ISSN (online)	1098-5522
ISSN	0019-9567
DOI	10.1128/iai.00223-23
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Zs.A 684: Show issues

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Article ; Online: Need for a Cardiogenic Shock Team Collaborative-Promoting a Team-Based Model of Care to Improve Outcomes and Identify Best Practices.

Senman, Balimkiz / Jentzer, Jacob C / Barnett, Christopher F / Bartos, Jason A / Berg, David D / Chih, Sharon / Drakos, Stavros G / Dudzinski, David M / Elliott, Andrea / Gage, Ann / Horowitz, James M / Miller, P Elliott / Sinha, Shashank S / Tehrani, Behnam N / Yuriditsky, Eugene / Vallabhajosyula, Saraschandra / Katz, Jason N

Journal of the American Heart Association

2024 Volume 13, Issue 6, Page(s) e031979

Abstract: Cardiogenic shock continues to carry a high mortality rate despite contemporary care, with no breakthrough therapies shown to improve survival over the past few decades. It is a time-sensitive condition that commonly results in cardiovascular ... ...

Abstract	Cardiogenic shock continues to carry a high mortality rate despite contemporary care, with no breakthrough therapies shown to improve survival over the past few decades. It is a time-sensitive condition that commonly results in cardiovascular complications and multisystem organ failure, necessitating multidisciplinary expertise. Managing patients with cardiogenic shock remains challenging even in well-resourced settings, and an important subgroup of patients may require cardiac replacement therapy. As a result, the idea of leveraging the collective cognitive and procedural proficiencies of multiple providers in a collaborative, team-based approach to care (the "shock team") has been advocated by professional societies and implemented at select high-volume clinical centers. A slowly maturing evidence base has suggested that cardiogenic shock teams may improve patient outcomes. Although several registries exist that are beginning to inform care, particularly around therapeutic strategies of pharmacologic and mechanical circulatory support, none of these are currently focused on the shock team approach, multispecialty partnership, education, or process improvement. We propose the creation of a Cardiogenic Shock Team Collaborative-akin to the successful Pulmonary Embolism Response Team Consortium-with a goal to promote sharing of care protocols, education of stakeholders, and discovery of how process and performance may influence patient outcomes, quality, resource consumption, and costs of care.
MeSH term(s)	Humans ; Shock, Cardiogenic/diagnosis ; Shock, Cardiogenic/therapy ; Shock, Cardiogenic/etiology
Language	English
Publishing date	2024-03-08
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2653953-6
ISSN	2047-9980 ; 2047-9980
ISSN (online)	2047-9980
ISSN	2047-9980
DOI	10.1161/JAHA.123.031979
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Article ; Online: Family carer experiences of hospice care at home: Qualitative findings from a mixed methods realist evaluation.

Abrahamson, Vanessa / Wilson, Patricia / Barclay, Stephen / Brigden, Charlotte / Gage, Heather / Greene, Kay / Hashem, Ferhana / Mikelyte, Rasa / Rees-Roberts, Melanie / Silsbury, Graham / Goodwin, Mary / Swash, Brooke / Wee, Bee / Williams, Peter / Butler, Claire

Palliative medicine

2023 Volume 37, Issue 10, Page(s) 1529–1539

Abstract: Background: Hospice-at-home aims to enable patients approaching end-of-life to die at home and support their carers. A wide range of different service models exists but synthesised evidence on how best to support family carers to provide sustainable end- ...

Abstract	Background: Hospice-at-home aims to enable patients approaching end-of-life to die at home and support their carers. A wide range of different service models exists but synthesised evidence on how best to support family carers to provide sustainable end-of-life care at home is limited. Aim: To explore what works best to promote family carers' experiences of hospice-at-home. Design: Realist evaluation with mixed methods. This paper focuses on qualitative interviews with carers (to gain their perspective and as proxy for patients) and service providers from 12 case study sites in England. Interviews were coded and programme theories were refined by the research team including two public members. Setting/participants: Interviews with carers (involved daily) of patients admitted to hospice-at-home services ( Results: Post bereavement, 76.4% of carers thought that they had received as much help and support as they needed and most carers (75.8%) rated the help and support as excellent or outstanding. Of six final programme theories capturing key factors relevant to providing optimum services, those directly relevant to carer experiences were: integration and co-ordination of services; knowledge, skills and ethos of hospice staff; volunteer roles; support directed at the patient-carer dyad. Conclusions: Carers in hospice-at-home services identified care to be of a higher quality than generic community services. Hospice staff were perceived as having 'time to care', communicated well and were comfortable with dying and death. Hands-on care was particularly valued in the period close to death.
MeSH term(s)	Humans ; Hospice Care ; Caregivers ; Home Care Services ; Palliative Care/methods ; Terminal Care
Language	English
Publishing date	2023-10-21
Publishing country	England
Document type	Journal Article
ZDB-ID	639247-7
ISSN	1477-030X ; 0269-2163
ISSN (online)	1477-030X
ISSN	0269-2163
DOI	10.1177/02692163231206027
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Zs.A 2285: Show issues

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Article ; Online: Improving Follow-up Attendance for Discharged Emergency Care Patients Using Automated Phone System to Self-schedule: A Randomized Controlled Trial.

Bauer, Kyla L / Sogade, Omolade O / Gage, Brian F / Ruoff, Brent / Lewis, Lawrence

Academic emergency medicine : official journal of the Society for Academic Emergency Medicine

2020 Volume 28, Issue 2, Page(s) 197–205

Abstract: Background: Automated phone appointment reminders have improved adherence with follow-up appointments in a variety of hospital settings, but have mixed results in patients discharged from the emergency department (ED). Increasing adherence to follow-up ... ...

Abstract	Background: Automated phone appointment reminders have improved adherence with follow-up appointments in a variety of hospital settings, but have mixed results in patients discharged from the emergency department (ED). Increasing adherence to follow-up care has been a priority in the ED to improve patient outcomes and reduce unnecessary future visits. Methods: We conducted a prospective randomized open, blinded end-point (PROBE) trial of 278 adult patients discharged from the ED and referred to a provider for follow-up care. Participants in the intervention arm received a self-scheduling text or phone message that automatically connected them to their referral provider to schedule a follow-up appointment and sent them appointment reminders. Those in the control arm received standard-of-care written instructions to contact listed referral providers. The primary outcome was time to appointment. The secondary outcome was time to return visit to the ED. Results: The automated reminders increased the cumulative incidence of keeping the referral appointment after ED discharge (p < 0.001, Gray's test). Of participants randomized to the automated phone intervention, 49.3% (n = 74) kept their follow-up appointment versus 23.4% (n = 30) in the control arm, with a hazard ratio (HR) and 95% confidence interval (CI) over the duration of the study period of 2.4 (1.6 to 3.7; p < 0.001). In a sensitivity analysis using 30 days of follow-up data, 42.0% (n = 63) of participants randomized to the phone intervention kept their follow-up versus 21.1% (n = 27) in the control arm, with a HR (95% CI) of 2.2 (1.4 to 3.5; p < 0.001). There was no difference in ED revisits between the intervention and control group within 120 days postdischarge. Conclusions: An automated self-scheduling phone system significantly improved follow-up adherence after ED discharge, but did not decrease ED revisits.
MeSH term(s)	Adult ; Aftercare ; Appointments and Schedules ; Emergency Service, Hospital ; Follow-Up Studies ; Humans ; Patient Discharge ; Prospective Studies ; Reminder Systems
Language	English
Publishing date	2020-08-05
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1329813-6
ISSN	1553-2712 ; 1069-6563
ISSN (online)	1553-2712
ISSN	1069-6563
DOI	10.1111/acem.14080
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Zs.A 4479: Show issues

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Article ; Online: Neuronal activity-related transcription is blunted in immature compared to mature dentate granule cells.

Parylak, Sarah L / Qiu, Fan / Linker, Sara B / Gallina, Iryna S / Lim, Christina K / Preciado, David / McDonald, Aidan H / Zhou, Xavier / Gage, Fred H

Hippocampus

2023 Volume 33, Issue 4, Page(s) 412–423

Abstract: Immature dentate granule cells (DGCs) generated in the hippocampus during adulthood are believed to play a unique role in dentate gyrus (DG) function. Although immature DGCs have hyperexcitable membrane properties in vitro, the consequences of this ... ...

Abstract	Immature dentate granule cells (DGCs) generated in the hippocampus during adulthood are believed to play a unique role in dentate gyrus (DG) function. Although immature DGCs have hyperexcitable membrane properties in vitro, the consequences of this hyperexcitability in vivo remain unclear. In particular, the relationship between experiences that activate the DG, such as exploration of a novel environment (NE), and downstream molecular processes that modify DG circuitry in response to cellular activation is unknown in this cell population. We first performed quantification of immediate early gene (IEG) proteins in immature (5-week-old) and mature (13-week-old) DGCs from mice exposed to a NE. Paradoxically, we observed lower IEG protein expression in hyperexcitable immature DGCs. We then isolated nuclei from active and inactive immature DGCs and performed single-nuclei RNA-Sequencing. Compared to mature nuclei collected from the same animal, immature DGC nuclei showed less activity-induced transcriptional change, even though they were classified as active based on expression of ARC protein. These results demonstrate that the coupling of spatial exploration, cellular activation, and transcriptional change differs between immature and mature DGCs, with blunted activity-induced changes in immature cells.
MeSH term(s)	Mice ; Animals ; Dentate Gyrus/physiology ; Neurons/physiology ; Hippocampus ; Neurogenesis/physiology
Language	English
Publishing date	2023-02-22
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1074352-2
ISSN	1098-1063 ; 1050-9631
ISSN (online)	1098-1063
ISSN	1050-9631
DOI	10.1002/hipo.23515
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Article ; Online: Novel chlorine‐extending polymer coating with prolonged antiviral activity against SARS‐CoV‐2

Zhang, Y. / Choi, Y. W. / Demir, B. / Ekbataniamiri, F. / Fulton, M. L. / Ma, M. / Schang, L. M. / Purevdorj‐Gage, L. / Qiao, M.

Letters in Applied Microbiology. 2022 Nov., v. 75, no. 5 p.1346-1353

2022

Abstract	We previously reported a novel polymeric surface coating, namely, HaloFilm™ that can immobilize and extend the antimicrobial activity of chlorine on surfaces. In this study, we demonstrated the continuous antiviral efficacy of HaloFilm when applied on stainless steel and cotton gauze as two representative models for non‐porous and porous surfaces against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Forty‐eight hours post HaloFilm application and chlorination and 2 h post the viral challenge, the inoculum titre was reduced by 2.25 ± 0.33 and ≥4.36 ± 0.23 log₁₀ TCID₅₀ on non‐porous and porous surfaces, respectively. The half‐life of the virus was shorter (13.86 min) on a HaloFilm‐coated surface than what has been reported on copper (46.44 min).
Keywords	Severe acute respiratory syndrome coronavirus 2 ; antiviral properties ; chlorination ; chlorine ; copper ; cotton ; half life ; inoculum ; polymers ; stainless steel ; viruses
Language	English
Dates of publication	2022-11
Size	p. 1346-1353.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	632584-1
ISSN	1472-765X ; 0266-8254
ISSN (online)	1472-765X
ISSN	0266-8254
DOI	10.1111/lam.13804
Database	NAL-Catalogue (AGRICOLA)

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