LIVIVO - Search results -

Search results

Result 1 - 10 of total 12

Search options

Article ; Online: Marker-based assays for studying gut transit in gnotobiotic and conventional mouse models.

Koester, Sean T / Li, Naisi / Lachance, Daniel M / Dey, Neelendu

STAR protocols

2021 Volume 2, Issue 4, Page(s) 100938

Abstract: Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of ... ...

Abstract	Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of the red carmine dye and fluorescence isothiocyanate-dextran marker-based assays for characterizing gut transit with spatial resolution, along with an optional intracolonic infusion protocol for studying the effects of metabolites on colonic transit. These protocols can be adapted for use in gnotobiotic and conventional mouse models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
MeSH term(s)	Animals ; Biomarkers/metabolism ; Disease Models, Animal ; Feces/chemistry ; Female ; Gastrointestinal Tract/chemistry ; Gastrointestinal Tract/metabolism ; Gastrointestinal Transit/physiology ; Germ-Free Life ; Male ; Mice
Chemical Substances	Biomarkers
Language	English
Publishing date	2021-11-05
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2021.100938
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Marker-based assays for studying gut transit in gnotobiotic and conventional mouse models

Sean T. Koester / Naisi Li / Daniel M. Lachance / Neelendu Dey

STAR Protocols, Vol 2, Iss 4, Pp 100938- (2021)

2021

Abstract: Summary: Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe ... ...

Abstract	Summary: Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of the red carmine dye and fluorescence isothiocyanate-dextran marker-based assays for characterizing gut transit with spatial resolution, along with an optional intracolonic infusion protocol for studying the effects of metabolites on colonic transit. These protocols can be adapted for use in gnotobiotic and conventional mouse models.For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
Keywords	Microbiology ; Model Organisms ; Science (General) ; Q1-390
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Microbiome-encoded bile acid metabolism modulates colonic transit times.

Li, Naisi / Koester, Sean T / Lachance, Daniel M / Dutta, Moumita / Cui, Julia Yue / Dey, Neelendu

iScience

2021 Volume 24, Issue 6, Page(s) 102508

Abstract: Gut motility is regulated by the microbiome via mechanisms that include bile acid metabolism. To localize the effects of microbiome-generated bile acids, we colonized gnotobiotic mice with different synthetic gut bacterial communities that were ... ...

Abstract	Gut motility is regulated by the microbiome via mechanisms that include bile acid metabolism. To localize the effects of microbiome-generated bile acids, we colonized gnotobiotic mice with different synthetic gut bacterial communities that were metabolically phenotyped using a functional
Language	English
Publishing date	2021-05-05
Publishing country	United States
Document type	Journal Article
ISSN	2589-0042
ISSN (online)	2589-0042
DOI	10.1016/j.isci.2021.102508
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Variability in digestive and respiratory tract Ace2 expression is associated with the microbiome.

Koester, Sean T / Li, Naisi / Lachance, Daniel M / Morella, Norma M / Dey, Neelendu

PloS one

2021 Volume 16, Issue 3, Page(s) e0248730

Abstract: COVID-19 (coronavirus disease 2019) patients exhibiting gastrointestinal symptoms are reported to have worse prognosis. Ace2 (angiotensin-converting enzyme 2), the gene encoding the host protein to which SARS-CoV-2 spike proteins bind, is expressed in ... ...

Abstract	COVID-19 (coronavirus disease 2019) patients exhibiting gastrointestinal symptoms are reported to have worse prognosis. Ace2 (angiotensin-converting enzyme 2), the gene encoding the host protein to which SARS-CoV-2 spike proteins bind, is expressed in the gut and therefore may be a target for preventing or reducing severity of COVID-19. Here we test the hypothesis that Ace2 expression in the gastrointestinal and respiratory tracts is modulated by the microbiome. We used quantitative PCR to profile Ace2 expression in germ-free mice, conventional raised specific pathogen-free mice, and gnotobiotic mice colonized with different microbiota. Intestinal Ace2 expression levels were significantly higher in germ-free mice compared to conventional mice. A similar trend was observed in the respiratory tract. Intriguingly, microbiota depletion via antibiotics partially recapitulated the germ-free phenotype, suggesting potential for microbiome-mediated regulation of Ace2 expression. Variability in intestinal Ace2 expression was observed in gnotobiotic mice colonized with different microbiota, partially attributable to differences in microbiome-encoded proteases and peptidases. Together, these data suggest that the microbiome may be one modifiable factor determining COVID-19 infection risk and disease severity.
MeSH term(s)	Adenomatous Polyposis Coli Protein/deficiency ; Adenomatous Polyposis Coli Protein/genetics ; Angiotensin-Converting Enzyme 2/genetics ; Angiotensin-Converting Enzyme 2/metabolism ; Animals ; Colon/enzymology ; Female ; Gastrointestinal Microbiome ; Gene Expression ; Interleukin-10/deficiency ; Interleukin-10/genetics ; Intestine, Small/enzymology ; Lung/enzymology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout
Chemical Substances	Adenomatous Polyposis Coli Protein ; adenomatous polyposis coli protein, mouse ; Interleukin-10 (130068-27-8) ; Ace2 protein, mouse (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
Language	English
Publishing date	2021-03-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0248730
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Variability in digestive and respiratory tract Ace2 expression is associated with the microbiome.

Sean T Koester / Naisi Li / Daniel M Lachance / Norma M Morella / Neelendu Dey

PLoS ONE, Vol 16, Iss 3, p e

2021 Volume 0248730

Abstract	COVID-19 (coronavirus disease 2019) patients exhibiting gastrointestinal symptoms are reported to have worse prognosis. Ace2 (angiotensin-converting enzyme 2), the gene encoding the host protein to which SARS-CoV-2 spike proteins bind, is expressed in the gut and therefore may be a target for preventing or reducing severity of COVID-19. Here we test the hypothesis that Ace2 expression in the gastrointestinal and respiratory tracts is modulated by the microbiome. We used quantitative PCR to profile Ace2 expression in germ-free mice, conventional raised specific pathogen-free mice, and gnotobiotic mice colonized with different microbiota. Intestinal Ace2 expression levels were significantly higher in germ-free mice compared to conventional mice. A similar trend was observed in the respiratory tract. Intriguingly, microbiota depletion via antibiotics partially recapitulated the germ-free phenotype, suggesting potential for microbiome-mediated regulation of Ace2 expression. Variability in intestinal Ace2 expression was observed in gnotobiotic mice colonized with different microbiota, partially attributable to differences in microbiome-encoded proteases and peptidases. Together, these data suggest that the microbiome may be one modifiable factor determining COVID-19 infection risk and disease severity.
Keywords	Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Microbiome-encoded bile acid metabolism modulates colonic transit times

Naisi Li / Sean T. Koester / Daniel M. Lachance / Moumita Dutta / Julia Yue Cui / Neelendu Dey

iScience, Vol 24, Iss 6, Pp 102508- (2021)

2021

Abstract: Summary: Gut motility is regulated by the microbiome via mechanisms that include bile acid metabolism. To localize the effects of microbiome-generated bile acids, we colonized gnotobiotic mice with different synthetic gut bacterial communities that were ... ...

Abstract	Summary: Gut motility is regulated by the microbiome via mechanisms that include bile acid metabolism. To localize the effects of microbiome-generated bile acids, we colonized gnotobiotic mice with different synthetic gut bacterial communities that were metabolically phenotyped using a functional in vitro screen. Using two different marker-based assays of gut transit, we inferred that bile acids exert effects on colonic transit. We validated this using an intra-colonic bile acid infusion assay and determined that these effects were dependent upon signaling via the bile acid receptor, TGR5. The intra-colonic bile acid infusion experiments further revealed sex-biased bile acid-specific effects on colonic transit, with lithocholic acid having the largest pro-motility effect. Transcriptional responses of the enteric nervous system (ENS) were stereotypic, regional, and observed in response to different microbiota, their associated bile acid profiles, and even to a single diet ingredient, evidencing exquisite sensitivity of the ENS to environmental perturbations.
Keywords	Microbiology ; Microbiome ; Gastroenterology ; Science ; Q
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Chemical Purification of Terbium-155 from Pseudo-Isobaric Impurities in a Mass Separated Source Produced at CERN.

Webster, Ben / Ivanov, Peter / Russell, Ben / Collins, Sean / Stora, Thierry / Ramos, Joao Pedro / Köster, Ulli / Robinson, Andrew Paul / Read, David

Scientific reports

2019 Volume 9, Issue 1, Page(s) 10884

Abstract: Four terbium radioisotopes ( ...

Abstract	Four terbium radioisotopes (
Language	English
Publishing date	2019-07-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-47463-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2.

Bolyen, Evan / Rideout, Jai Ram / Dillon, Matthew R / Bokulich, Nicholas A / Abnet, Christian C / Al-Ghalith, Gabriel A / Alexander, Harriet / Alm, Eric J / Arumugam, Manimozhiyan / Asnicar, Francesco / Bai, Yang / Bisanz, Jordan E / Bittinger, Kyle / Brejnrod, Asker / Brislawn, Colin J / Brown, C Titus / Callahan, Benjamin J / Caraballo-Rodríguez, Andrés Mauricio / Chase, John /

Cope, Emily K / Da Silva, Ricardo / Diener, Christian / Dorrestein, Pieter C / Douglas, Gavin M / Durall, Daniel M / Duvallet, Claire / Edwardson, Christian F / Ernst, Madeleine / Estaki, Mehrbod / Fouquier, Jennifer / Gauglitz, Julia M / Gibbons, Sean M / Gibson, Deanna L / Gonzalez, Antonio / Gorlick, Kestrel / Guo, Jiarong / Hillmann, Benjamin / Holmes, Susan / Holste, Hannes / Huttenhower, Curtis / Huttley, Gavin A / Janssen, Stefan / Jarmusch, Alan K / Jiang, Lingjing / Kaehler, Benjamin D / Kang, Kyo Bin / Keefe, Christopher R / Keim, Paul / Kelley, Scott T / Knights, Dan / Koester, Irina / Kosciolek, Tomasz / Kreps, Jorden / Langille, Morgan G I / Lee, Joslynn / Ley, Ruth / Liu, Yong-Xin / Loftfield, Erikka / Lozupone, Catherine / Maher, Massoud / Marotz, Clarisse / Martin, Bryan D / McDonald, Daniel / McIver, Lauren J / Melnik, Alexey V / Metcalf, Jessica L / Morgan, Sydney C / Morton, Jamie T / Naimey, Ahmad Turan / Navas-Molina, Jose A / Nothias, Louis Felix / Orchanian, Stephanie B / Pearson, Talima / Peoples, Samuel L / Petras, Daniel / Preuss, Mary Lai / Pruesse, Elmar / Rasmussen, Lasse Buur / Rivers, Adam / Robeson, Michael S / Rosenthal, Patrick / Segata, Nicola / Shaffer, Michael / Shiffer, Arron / Sinha, Rashmi / Song, Se Jin / Spear, John R / Swafford, Austin D / Thompson, Luke R / Torres, Pedro J / Trinh, Pauline / Tripathi, Anupriya / Turnbaugh, Peter J / Ul-Hasan, Sabah / van der Hooft, Justin J J / Vargas, Fernando / Vázquez-Baeza, Yoshiki / Vogtmann, Emily / von Hippel, Max / Walters, William / Wan, Yunhu / Wang, Mingxun / Warren, Jonathan / Weber, Kyle C / Williamson, Charles H D / Willis, Amy D / Xu, Zhenjiang Zech / Zaneveld, Jesse R / Zhang, Yilong / Zhu, Qiyun / Knight, Rob / Caporaso, J Gregory

Nature biotechnology

2019 Volume 37, Issue 8, Page(s) 852–857

MeSH term(s)	Computational Biology ; Data Science ; Databases, Factual ; Humans ; Microbiota ; Software
Language	English
Publishing date	2019-10-15
Publishing country	United States
Document type	Letter ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1311932-1
ISSN	1546-1696 ; 1087-0156
ISSN (online)	1546-1696
ISSN	1087-0156
DOI	10.1038/s41587-019-0209-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2167: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 137: Show issues
Zs.MG 43: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Author Correction: Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2.

Nature biotechnology

2019 Volume 37, Issue 9, Page(s) 1091

Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

Abstract	An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Language	English
Publishing date	2019-08-13
Publishing country	United States
Document type	Published Erratum
ZDB-ID	1311932-1
ISSN	1546-1696 ; 1087-0156
ISSN (online)	1546-1696
ISSN	1087-0156
DOI	10.1038/s41587-019-0252-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2167: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 137: Show issues
Zs.MG 43: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2

Bolyen, Evan / Rideout, Jai Ram / Dillon, Matthew R. / Bokulich, Nicholas A. / Abnet, Christian C. / Al-Ghalith, Gabriel A. / Alexander, Harriet / Alm, Eric J. / Arumugam, Manimozhiyan / Asnicar, Francesco / Bai, Yang / Bisanz, Jordan E. / Bittinger, Kyle / Brejnrod, Asker / Brislawn, Colin J. / Brown, Titus C. / Callahan, Benjamin J. / Caraballo-Rodríguez, Andrés Mauricio / Chase, John /

Cope, Emily K. / da Silva, Ricardo / Diener, Christian / Dorrestein, Pieter C. / Douglas, Gavin M. / Durall, Daniel M. / Duvallet, Claire / Edwardson, Christian F. / Ernst, Madeleine / Estaki, Mehrbod / Fouquier, Jennifer / Gauglitz, Julia M. / Gibbons, Sean M. / Gibson, Deanna L. / Gonzalez, Antonio / Gorlick, Kestrel / Guo, Jiarong / Hillmann, Benjamin / Holmes, Susan / Holste, Hannes / Huttenhower, Curtis / Huttley, Gavin A. / Janssen, Stefan / Jarmusch, Alan K. / Jiang, Lingjing / Kaehler, Benjamin D. / Kang, Kyo Bin / Keefe, Christopher R. / Keim, Paul / Kelley, Scott T. / Knights, Dan / Koester, Irina / Kosciolek, Tomasz / Kreps, Jorden / Langille, Morgan G.I. / Lee, Joslynn / Ley, Ruth / Liu, Yong Xin / Loftfield, Erikka / Lozupone, Catherine / Maher, Massoud / Marotz, Clarisse / Martin, Bryan D. / McDonald, Daniel / McIver, Lauren J. / Melnik, Alexey V. / Metcalf, Jessica L. / Morgan, Sydney C. / Morton, Jamie T. / Naimey, Ahmad Turan / Navas-Molina, Jose A. / Nothias, Louis Felix / Orchanian, Stephanie B. / Pearson, Talima / Peoples, Samuel L. / Petras, Daniel / Preuss, Mary Lai / Pruesse, Elmar / Rasmussen, Lasse Buur / Rivers, Adam / Robeson, Michael S. / Rosenthal, Patrick / Segata, Nicola / Shaffer, Michael / Shiffer, Arron / Sinha, Rashmi / Song, Se Jin / Spear, John R. / Swafford, Austin D. / Thompson, Luke R. / Torres, Pedro J. / Trinh, Pauline / Tripathi, Anupriya / Turnbaugh, Peter J. / Ul-Hasan, Sabah / van der Hooft, Justin J.J. / Vargas, Fernando / Vázquez-Baeza, Yoshiki / Vogtmann, Emily / von Hippel, Max / Walters, William / Wan, Yunhu / Wang, Mingxun / Warren, Jonathan / Weber, Kyle C. / Williamson, Charles H.D. / Willis, Amy D. / Xu, Zhenjiang Zech / Zaneveld, Jesse R. / Zhang, Yilong / Zhu, Qiyun / Knight, Rob / Caporaso, J.G.

Nature Biotechnology

2019 Volume 37, Issue 8

Keywords	Life Science
Language	English
Publishing country	nl
Document type	Article ; Online
ZDB-ID	1311932-1
ISSN	1546-1696 ; 1087-0156
ISSN (online)	1546-1696
ISSN	1087-0156
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

In stock of ZB MED Cologne/Königswinter

Zs.A 2167: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 137: Show issues
Zs.MG 43: Show issues

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito