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  1. Article ; Online: Improving the quality of antenatal screening and early intervention for alcohol and other drug use: protocol for a multi-stage approach to systems reform.

    Stevens, Matthew W R / Cooper, Megan / Cusack, Lynette / Ali, Robert L / Briley, Annette L

    Addiction science & clinical practice

    2024  Volume 19, Issue 1, Page(s) 2

    Abstract: Background: Alcohol, tobacco and illicit drug use during pregnancy can cause significant harm to women and their developing fetuses. Despite recommendations for abstinence during pregnancy, some women continue to use, making screening for substance use ... ...

    Abstract Background: Alcohol, tobacco and illicit drug use during pregnancy can cause significant harm to women and their developing fetuses. Despite recommendations for abstinence during pregnancy, some women continue to use, making screening for substance use during antenatal clinic attendances an important strategy for reducing risk. This study aims to improve the rates of screening and intervention for substance use among pregnant women, including appropriate referral for those who may be substance-dependent. The protocol outlined here focuses on a multi-stage implementation study.
    Methods: This study will occur in four phases. Phase 1 will identify a baseline rate of screening and subsequent care at the antenatal clinics of two, South Australian hospital-based maternity services, through a retrospective case note audit. Rates of self-reported substance use identified in the case notes will also be compared against representative data from Adelaide Primary Health Network to establish rates of over or underreporting. Phase 2 will involve an online Training Needs Analysis of midwifery staff working at those services, to assess their knowledge, attitudes, beliefs, and commitment to the care of women who use substances during pregnancy. Phase 3 will involve a training package for all midwifery staff at those services, focused on routine screening for substance use, and how to provide appropriate care. Outcome measures from phase 2 will be reassessed during phase 3 and any changes since training will be evaluated. Phase 4 will then repeat phase 1 to compare the changes in rates of both screening and any associated intervention before and after training.
    Discussion: From a public health perspective, this project has the potential to make a significant impact on reducing risk of harm from substance use disorders among pregnant women, and contribute to better health outcomes for their children.
    Trial registration:  This trial has been pre-registered under the Open Science Framework.
    Registration: https://doi.org/10.17605/OSF.IO/73FDZ .
    MeSH term(s) Pregnancy ; Child ; Female ; Humans ; Retrospective Studies ; Australia ; Ethanol ; Prenatal Diagnosis ; Substance-Related Disorders/diagnosis ; Substance-Related Disorders/therapy
    Chemical Substances Ethanol (3K9958V90M)
    Language English
    Publishing date 2024-01-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2492632-2
    ISSN 1940-0640 ; 1940-0640
    ISSN (online) 1940-0640
    ISSN 1940-0640
    DOI 10.1186/s13722-023-00434-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: A repositioning screen using an FGFR2 splicing reporter reveals compounds that regulate epithelial-mesenchymal transitions and inhibit growth of prostate cancer xenografts.

    Li, Ling / Zheng, Jinxia / Stevens, Megan / Oltean, Sebastian

    Molecular therapy. Methods & clinical development

    2022  Volume 25, Page(s) 147–157

    Abstract: Research in the area of hallmarks of cancer has opened the possibility of designing new therapies based on modulating these cancer properties. We present here a screen designed to find chemicals that modulate epithelial-mesenchymal transitions (EMTs) in ... ...

    Abstract Research in the area of hallmarks of cancer has opened the possibility of designing new therapies based on modulating these cancer properties. We present here a screen designed to find chemicals that modulate epithelial-mesenchymal transitions (EMTs) in prostate cancer. For screening, we used a repurposing library and, as a readout, an FGFR2-based splicing reporter, which has been shown previously to be a sensor for EMTs. Various properties of cancer cells were assessed, signaling pathways investigated, and
    Language English
    Publishing date 2022-03-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2872938-9
    ISSN 2329-0501 ; 2329-0501
    ISSN (online) 2329-0501
    ISSN 2329-0501
    DOI 10.1016/j.omtm.2022.03.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Improving the quality of antenatal screening and early intervention for alcohol and other drug use

    Matthew W. R. Stevens / Megan Cooper / Lynette Cusack / Robert L. Ali / Annette L. Briley

    Addiction Science & Clinical Practice, Vol 19, Iss 1, Pp 1-

    protocol for a multi-stage approach to systems reform

    2024  Volume 8

    Abstract: Abstract Background Alcohol, tobacco and illicit drug use during pregnancy can cause significant harm to women and their developing fetuses. Despite recommendations for abstinence during pregnancy, some women continue to use, making screening for ... ...

    Abstract Abstract Background Alcohol, tobacco and illicit drug use during pregnancy can cause significant harm to women and their developing fetuses. Despite recommendations for abstinence during pregnancy, some women continue to use, making screening for substance use during antenatal clinic attendances an important strategy for reducing risk. This study aims to improve the rates of screening and intervention for substance use among pregnant women, including appropriate referral for those who may be substance-dependent. The protocol outlined here focuses on a multi-stage implementation study. Methods This study will occur in four phases. Phase 1 will identify a baseline rate of screening and subsequent care at the antenatal clinics of two, South Australian hospital-based maternity services, through a retrospective case note audit. Rates of self-reported substance use identified in the case notes will also be compared against representative data from Adelaide Primary Health Network to establish rates of over or underreporting. Phase 2 will involve an online Training Needs Analysis of midwifery staff working at those services, to assess their knowledge, attitudes, beliefs, and commitment to the care of women who use substances during pregnancy. Phase 3 will involve a training package for all midwifery staff at those services, focused on routine screening for substance use, and how to provide appropriate care. Outcome measures from phase 2 will be reassessed during phase 3 and any changes since training will be evaluated. Phase 4 will then repeat phase 1 to compare the changes in rates of both screening and any associated intervention before and after training. Discussion From a public health perspective, this project has the potential to make a significant impact on reducing risk of harm from substance use disorders among pregnant women, and contribute to better health outcomes for their children. Trial registration: This trial has been pre-registered under the Open Science Framework. Registration: ...
    Keywords Medicine (General) ; R5-920 ; Social pathology. Social and public welfare. Criminology ; HV1-9960
    Subject code 360
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Detection of genomic mutations in blood and urine free circulating tumour DNA in patients with inoperable and metastatic lung adenocarcinoma harbouring an

    Brooks, Helen / Li, Ling / Addeo, Alfredo / Stevens, Megan / Comins, Charles / Oltean, Sebastian

    Frontiers in oncology

    2023  Volume 13, Page(s) 1197037

    Abstract: The development of methodologies to analyse circulating tumour DNA (ctDNA) in the blood or urine of cancer patients provides an invaluable resource that can be used for diagnosis and prognosis and to evaluate response to treatments. Lung cancer has seen ... ...

    Abstract The development of methodologies to analyse circulating tumour DNA (ctDNA) in the blood or urine of cancer patients provides an invaluable resource that can be used for diagnosis and prognosis and to evaluate response to treatments. Lung cancer has seen in the last years a revolution in treatment strategy with the use of several classes of EGFR inhibitors. However, almost invariably, resistance to such therapies appears. In this paper, we describe a pilot, longitudinal study with 20 patients with confirmed
    Language English
    Publishing date 2023-07-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2023.1197037
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Temporal parameters of enhanced opioid reward after initial opioid exposure in rats.

    Moerke, Megan J / Negus, S Stevens

    Psychopharmacology

    2021  Volume 238, Issue 3, Page(s) 725–734

    Abstract: Rationale: Mu opioid receptor agonists are indispensable for the treatment of pain, but clinical use carries the inherent risk of transition from effective treatment to abuse. Abuse potential appears to increase rapidly during periods of initial opioid ... ...

    Abstract Rationale: Mu opioid receptor agonists are indispensable for the treatment of pain, but clinical use carries the inherent risk of transition from effective treatment to abuse. Abuse potential appears to increase rapidly during periods of initial opioid exposure in humans, and this increase in opioid reward during initial opioid exposure can be modeled in rats using an intracranial self-stimulation (ICSS) procedure.
    Objectives: The goal of the present study was to examine temporal parameters of this phenomenon.
    Methods: Adult male Sprague-Dawley rats responded for electrical brain stimulation using a frequency-rate ICSS procedure. In the first experiment, rats received daily morphine injections for 6 days, and morphine effects on ICSS were re-determined 1 day, 1 week, or 1 month after the repeated morphine treatment regimen to evaluate the persistence of enhanced opioid reward. In the second experiment, rats received six repeated morphine injections with different interdose intervals (two per day, one per day, every other day, every fourth day), and morphine effects were re-determined 1 day after the last dose to determine dosing frequencies sufficient to produce enhanced opioid reward.
    Results: Results of the first experiment indicated that enhanced opioid reward was greatest 1 day after the morphine treatment regimen and completely dissipated after 4 weeks. The second experiment indicated that all dosing frequencies tested were sufficient to produce enhanced reward.
    Conclusions: Taken together, these results suggest that enhancement of opioid reward after initial opioid exposure is relatively transient but can be produced by a range of different dosing frequencies.
    MeSH term(s) Analgesics, Opioid/administration & dosage ; Analgesics, Opioid/pharmacology ; Animals ; Brain/drug effects ; Brain/metabolism ; Electric Stimulation ; Male ; Morphine/administration & dosage ; Morphine/pharmacology ; Pain/drug therapy ; Pain/metabolism ; Pain/psychology ; Rats ; Rats, Sprague-Dawley ; Receptors, Opioid, mu/agonists ; Reward ; Self Stimulation/drug effects ; Time Factors
    Chemical Substances Analgesics, Opioid ; Receptors, Opioid, mu ; Morphine (76I7G6D29C)
    Language English
    Publishing date 2021-01-07
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 130601-7
    ISSN 1432-2072 ; 0033-3158
    ISSN (online) 1432-2072
    ISSN 0033-3158
    DOI 10.1007/s00213-020-05725-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Effects of naltrexone on amphetamine choice in rhesus monkeys and rats.

    Robinson, Hannah L / Moerke, Megan Jo / Banks, Matthew L / Negus, S Stevens

    Experimental and clinical psychopharmacology

    2023  Volume 31, Issue 6, Page(s) 1080–1091

    Abstract: Clinical amphetamine use is constrained by high abuse potential, and amphetamine use disorder is a persistent clinical problem with no approved medications for its treatment. The opioid antagonist naltrexone has been reported to reduce some abuse-related ...

    Abstract Clinical amphetamine use is constrained by high abuse potential, and amphetamine use disorder is a persistent clinical problem with no approved medications for its treatment. The opioid antagonist naltrexone has been reported to reduce some abuse-related effects of amphetamine. This study used an amphetamine-versus-food choice procedure in rhesus monkeys and rats to test the hypothesis that naltrexone might serve as either (a) a maintenance medication for amphetamine use disorder treatment or (b) an "abuse-deterrent" adjunct to clinical amphetamine formulations. Male rhesus monkeys and male and female rats were trained to choose between increasing unit doses of intravenous amphetamine and an alternative food reinforcer during daily behavioral sessions. Experiment 1 evaluated effectiveness of continuous naltrexone maintenance to reduce amphetamine-versus-food choice in both monkeys and rats. Experiment 2 combined naltrexone with amphetamine in fixed-proportion amphetamine + naltrexone mixtures to evaluate the effectiveness of naltrexone in both species to reduce mixture choice relative to amphetamine-alone choice. Amphetamine maintained a dose-dependent increase in amphetamine choice in both monkeys and rats. Naltrexone maintenance did not significantly decrease amphetamine choice in either species. Addition of naltrexone to amphetamine reduced amphetamine choices per session in monkeys, but behavior was not reallocated to food choice, and in rats, the addition of naltrexone only decreased food choice without significantly affecting amphetamine choice. These results argue against the use of naltrexone as either (a) a maintenance medication for treatment of amphetamine use disorder or (b) an "abuse-deterrent" adjunct to amphetamine for clinical applications. (PsycInfo Database Record (c) 2023 APA, all rights reserved).
    MeSH term(s) Male ; Female ; Rats ; Animals ; Amphetamine/pharmacology ; Naltrexone/pharmacology ; Naltrexone/therapeutic use ; Macaca mulatta ; Cocaine ; Narcotic Antagonists/pharmacology ; Narcotic Antagonists/therapeutic use ; Substance-Related Disorders/drug therapy ; Choice Behavior ; Dose-Response Relationship, Drug ; Self Administration
    Chemical Substances Amphetamine (CK833KGX7E) ; Naltrexone (5S6W795CQM) ; Cocaine (I5Y540LHVR) ; Narcotic Antagonists
    Language English
    Publishing date 2023-05-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1209960-0
    ISSN 1936-2293 ; 1064-1297
    ISSN (online) 1936-2293
    ISSN 1064-1297
    DOI 10.1037/pha0000655
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: He did it! Or did I just see him on Twitter? Social media influence on eyewitness identification.

    Kleider-Offutt, Heather M / Stevens, Beth B / Capodanno, Megan

    Memory (Hove, England)

    2021  Volume 30, Issue 4, Page(s) 493–504

    Abstract: Eyewitness identification is fallible, and suggestive post-event information is known to facilitate error; however, whether social media valence affects identification decisions is unknown. After viewing crime videos of various race perpetrators, ... ...

    Abstract Eyewitness identification is fallible, and suggestive post-event information is known to facilitate error; however, whether social media valence affects identification decisions is unknown. After viewing crime videos of various race perpetrators, participants saw post-event Twitter photos of the perpetrator or a foil that varied in valence. Participants attempted identification from a lineup including both individuals and rated the confidence and source (i.e., video, Twitter) of their selection. Results showed that Twitter photos of the perpetrator increased the likelihood of accurate identification and related confidence, whereas seeing the foil reduced the likelihood of a correct identification and related confidence. Remembering the perpetrator from the crime influenced correct identification, while remembering other incorrect sources (e.g., Twitter) only lead to misidentification. Twitter valence and perpetrator race did not impact outcomes. Results suggest that difficulty in identifying a perpetrator is underpinned by source monitoring confusion which is exacerbated by viewing social media that includes innocent suspects.
    MeSH term(s) Crime ; Humans ; Male ; Mental Recall ; Recognition, Psychology ; Social Media
    Language English
    Publishing date 2021-07-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 1147478-6
    ISSN 1464-0686 ; 0965-8211
    ISSN (online) 1464-0686
    ISSN 0965-8211
    DOI 10.1080/09658211.2021.1953080
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Modulation of Receptor Tyrosine Kinase Activity through Alternative Splicing of Ligands and Receptors in the VEGF-A/VEGFR Axis.

    Stevens, Megan / Oltean, Sebastian

    Cells

    2019  Volume 8, Issue 4

    Abstract: Vascular endothelial growth factor A (VEGF-A) signaling is essential for physiological and pathological angiogenesis. Alternative splicing of the VEGF-A pre-mRNA gives rise to a pro-angiogenic family of isoforms with a differing number of amino acids ( ... ...

    Abstract Vascular endothelial growth factor A (VEGF-A) signaling is essential for physiological and pathological angiogenesis. Alternative splicing of the VEGF-A pre-mRNA gives rise to a pro-angiogenic family of isoforms with a differing number of amino acids (VEGF-A
    MeSH term(s) Alternative Splicing/genetics ; Animals ; Humans ; Ligands ; Neovascularization, Physiologic/genetics ; Receptors, Vascular Endothelial Growth Factor/genetics ; Signal Transduction ; Vascular Endothelial Growth Factor A/genetics
    Chemical Substances Ligands ; Vascular Endothelial Growth Factor A ; Receptors, Vascular Endothelial Growth Factor (EC 2.7.10.1)
    Language English
    Publishing date 2019-03-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells8040288
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Profiling cognitive workload in an unmanned vehicle control task with cognitive models and physiological metrics.

    Stevens, Christopher A / Morris, Megan B / Fisher, Christopher R / Myers, Christopher W

    Military psychology : the official journal of the Division of Military Psychology, American Psychological Association

    2022  Volume 35, Issue 6, Page(s) 507–520

    Abstract: In the present study, we use Cognitive Metrics Profiling (CMP) to capture variance in cognitive load within a complex unmanned vehicle control task. We aim to demonstrate convergent validity with existing workload measurement methods, and to decompose ... ...

    Abstract In the present study, we use Cognitive Metrics Profiling (CMP) to capture variance in cognitive load within a complex unmanned vehicle control task. We aim to demonstrate convergent validity with existing workload measurement methods, and to decompose workload into constituent cognitive resources to aid in diagnosing causes of workload. A cognitive model of the task was developed and examined to determine the extent to which it could predict behavioral performance, subjective workload, and validated physiological workload metrics. We also examined model activity to draw insights regarding loaded cognitive capacities. We found that composite workload from the model predicted physiological metrics, performance, and subjective workload. Moreover, the model indicates that differences in workload were driven largely by procedural, declarative, and temporal memory demands. We have found preliminary evidence of correspondence between workload predictions of a CMP model and physiological measures of workload. This suggests our approach captures interesting aspects of workload in a complex task environment and may provide a theoretical link between behavioral, physiological, and subjective metrics. This approach may provide a means to design effective workload mitigation interventions and improve decision-making about personnel tasking and automation.
    MeSH term(s) Task Performance and Analysis ; Workload/psychology ; Automation ; Cognition
    Language English
    Publishing date 2022-10-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2021130-2
    ISSN 1532-7876 ; 0899-5605
    ISSN (online) 1532-7876
    ISSN 0899-5605
    DOI 10.1080/08995605.2022.2130673
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Modulation of the Apoptosis Gene Bcl-x Function Through Alternative Splicing.

    Stevens, Megan / Oltean, Sebastian

    Frontiers in genetics

    2019  Volume 10, Page(s) 804

    Abstract: Apoptosis plays a vital role in cell homeostasis during development and disease. Bcl-x, a member of the Bcl-2 family of proteins, is a mitochondrial transmembrane protein that functions to regulate the intrinsic apoptosis pathway. An alternative splicing ...

    Abstract Apoptosis plays a vital role in cell homeostasis during development and disease. Bcl-x, a member of the Bcl-2 family of proteins, is a mitochondrial transmembrane protein that functions to regulate the intrinsic apoptosis pathway. An alternative splicing (AS) event in exon 2 of Bcl-x results in two isoforms of Bcl-x with antagonistic effects on cell survival: Bcl-xL (long isoform), which is anti-apoptotic, and Bcl-xS (short isoform), which is pro-apoptotic. Bcl-xL is the most abundant Bcl-x protein and functions to inhibit apoptosis by a number of different mechanisms including inhibition of Bax. In contrast, Bcl-xS can directly bind to and inhibit the anti-apoptotic Bcl-xL and Bcl-2 proteins, resulting in the release of the pro-apoptotic Bak. There are multiple splice factors and signaling pathways that influence the Bcl-xL/Bcl-xS splicing ratio, including serine/arginine-rich (SR) proteins, heterogeneous nuclear ribonucleoproteins (hnRNPs), transcription factors, and cytokines. Dysregulation of the AS of Bcl-x has been implicated in cancer and diabetes. In cancer, the upregulation of Bcl-xL expression in tumor cells can result in resistance to chemotherapeutic agents. On the other hand, dysregulation of Bcl-x AS to promote Bcl-xS expression has been shown to be detrimental to pancreatic β-cells in diabetes, resulting in β-cell apoptosis. Therefore, manipulation of the splice factor, transcription factor, and signaling pathways that modulate this splicing event is fast emerging as a therapeutic avenue in the treatment of cancer and diabetes.
    Language English
    Publishing date 2019-09-06
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2019.00804
    Database MEDical Literature Analysis and Retrieval System OnLINE

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