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  1. Article ; Online: Mouse Brain Tissue Preparation for Scanning Electron Microscopy.

    Mori, Kosuke / Takada, Chihiro / Okada, Hideshi / Tomita, Hiroyuki

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2794, Page(s) 63–70

    Abstract: Scanning electron microscopy (SEM) is used to observe the surface structure of an object by irradiating an electron beam onto the sample and detecting the reflected and emitted electrons. Because of its large depth of focus, SEM can provide the three- ... ...

    Abstract Scanning electron microscopy (SEM) is used to observe the surface structure of an object by irradiating an electron beam onto the sample and detecting the reflected and emitted electrons. Because of its large depth of focus, SEM can provide the three-dimensional structure of small surfaces that cannot be observed using an optical microscope. Furthermore, the cross-sectional structure of the tissue can be observed by freeze-cracking. Observing the ultrastructure of organisms that contain large amounts of water in their bodies while maintaining high resolution is challenging; however, this has recently become possible. Here, we explain the fixation and freeze-cracking method for mouse brain samples.
    MeSH term(s) Animals ; Mice ; Microscopy, Electron, Scanning ; Cross-Sectional Studies ; Electrons ; Histological Techniques ; Brain
    Language English
    Publishing date 2024-04-17
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3810-1_6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Editorial: Vascular Endothelial Glycocalyx in Cardiovascular Disease.

    Tomita, Hiroyuki / Suzuki, Kodai / Komatsu, Masanobu / Okada, Hideshi

    Frontiers in cardiovascular medicine

    2022  Volume 9, Page(s) 952022

    Language English
    Publishing date 2022-07-01
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2781496-8
    ISSN 2297-055X
    ISSN 2297-055X
    DOI 10.3389/fcvm.2022.952022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The endothelial glycocalyx-All the same? No, it is not.

    Suzuki, Kodai / Miura, Tomotaka / Okada, Hideshi

    Acute medicine & surgery

    2023  Volume 10, Issue 1, Page(s) e896

    Abstract: The endothelial glycocalyx covers the lumen of blood vessels throughout the body and plays an important role in endothelial homeostasis. Advances in electron microscopy techniques have provided clues to better understand the structure and composition of ... ...

    Abstract The endothelial glycocalyx covers the lumen of blood vessels throughout the body and plays an important role in endothelial homeostasis. Advances in electron microscopy techniques have provided clues to better understand the structure and composition of identical vascular endothelial glycocalyx. The morphology and thickness of the endothelial glycocalyx differ from organ to organ. The content of the endothelial glycocalyx covering the vascular lumen differs even in the brain, heart, and lungs, which have the same continuous capillaries. Various types of inflammation are known to attenuate the endothelial glycocalyx; however, we found that the morphology of the glycocalyx damaged by acute inflammation differed from that damaged by chronic inflammation. Acute inflammation breaks the endothelial glycocalyx unevenly, whereas chronic inflammation leads to the overall shortening of the endothelial glycocalyx. The same drug has different effects on the endothelial glycocalyx, depending on the location of the target blood vessels. This difference in response may reflect not only the size and shape of the endothelial glycocalyx but also the different constituents. In the cardiac tissue, the expression of glypican-1, a core protein of the endothelial glycocalyx, was enhanced. By contrast, in the pulmonary tissue, the expression of heparan sulfate 6-O-sulfotransferase 1 and endothelial cell-specific molecule-1 significantly increased in the treatment group compared with that in the no-treatment group. In this review, we present the latest findings on the evolution of the vascular endothelial glycocalyx and consider the microstructural differences.
    Language English
    Publishing date 2023-10-04
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2751184-4
    ISSN 2052-8817 ; 2052-8817
    ISSN (online) 2052-8817
    ISSN 2052-8817
    DOI 10.1002/ams2.896
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  4. Article ; Online: Editorial

    Hiroyuki Tomita / Kodai Suzuki / Masanobu Komatsu / Hideshi Okada

    Frontiers in Cardiovascular Medicine, Vol

    Vascular Endothelial Glycocalyx in Cardiovascular Disease

    2022  Volume 9

    Keywords endothelium ; endothelial glycocalyx ; cardiovascular disease ; vascular biology ; microcirculation ; Diseases of the circulatory (Cardiovascular) system ; RC666-701
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Glycocalyx analysis of bladder cancer: three-dimensional images in electron microscopy and vicia villosa lectin as a marker for invasiveness in frozen sections.

    Enomoto, Torai / Okada, Hideshi / Tomita, Hiroyuki / Iinuma, Koji / Nakane, Keita / Tobisawa, Yuki / Hara, Akira / Koie, Takuya

    Frontiers in cell and developmental biology

    2024  Volume 11, Page(s) 1308879

    Abstract: Introduction: ...

    Abstract Introduction:
    Language English
    Publishing date 2024-01-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2023.1308879
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  6. Article ; Online: Form follows function: The endothelial glycocalyx.

    Suzuki, Akio / Tomita, Hiroyuki / Okada, Hideshi

    Translational research : the journal of laboratory and clinical medicine

    2022  Volume 247, Page(s) 158–167

    Abstract: Three types of capillaries, namely continuous, fenestrated, and sinusoidal, form the microvascular system; each type has a specialized structure and function to respond to the demands of the organs they supply. The endothelial glycocalyx, a gel-like ... ...

    Abstract Three types of capillaries, namely continuous, fenestrated, and sinusoidal, form the microvascular system; each type has a specialized structure and function to respond to the demands of the organs they supply. The endothelial glycocalyx, a gel-like layer of glycoproteins that covers the luminal surface of the capillary endothelium, is also thought to maintain organ and vascular homeostasis by exhibiting different morphologies based on the functions of the organs and capillaries in which it is found. Recent advances in analytical technology have enabled more detailed observations of the endothelial glycocalyx, revealing that it indeed differs in structure across various organs. Furthermore, differences in the lectin staining patterns suggest the presence of different endothelial glycocalyx components across various organs. Interestingly, injury to the endothelial glycocalyx due to various pathologic and physiological stimuli causes the release of these components into the blood. Thus, circulating glycocalyx components may be useful biomarkers of organ dysfunction and disease severity. Moreover, a recent study suggested that chronic injury to the glycocalyx reduces the production of these glycocalyx components and changes their structure, leading it to become more vulnerable to external stimuli. In this review, we have summarized the various endothelial glycocalyx structures and their functions.
    MeSH term(s) Biomarkers ; Endothelium, Vascular/pathology ; Glycocalyx/pathology ; Glycocalyx/physiology
    Chemical Substances Biomarkers
    Language English
    Publishing date 2022-04-11
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2246684-8
    ISSN 1878-1810 ; 1532-6543 ; 1931-5244
    ISSN (online) 1878-1810 ; 1532-6543
    ISSN 1931-5244
    DOI 10.1016/j.trsl.2022.03.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  7. Article: Right subclavian artery injury during catheter insertion into the right internal jugular vein treated with endovascular stent graft placement after balloon occlusion test: A case report.

    Tamaoki, Yuto / Kamidani, Ryo / Okada, Hideshi / Miyake, Takahito / Suzuki, Kodai / Yoshida, Takahiro / Kumada, Keisuke / Yoshida, Shozo / Ogura, Shinji

    Radiology case reports

    2024  Volume 19, Issue 7, Page(s) 2579–2584

    Abstract: Subclavian artery injuries during internal jugular vein puncture when attempting central venous catheter insertion are rare. A 60-year-old man undergoing treatment for neuromyelitis optica with paralysis and sensory loss developed a complication during ... ...

    Abstract Subclavian artery injuries during internal jugular vein puncture when attempting central venous catheter insertion are rare. A 60-year-old man undergoing treatment for neuromyelitis optica with paralysis and sensory loss developed a complication during catheter placement into his right internal jugular vein for plasmapheresis. His previous physician felt resistance and discontinued the procedure. The patient later developed mild dyspnea and dysphagia. Computed tomography scans indicated thrombus formation and tracheal deviation. Contrast-enhanced computed tomography scans showed right subclavian artery injury with extravasation and a large pseudoaneurysm. Following transferal to our hospital, he was stable and asymptomatic; however, contrast-enhanced computed tomography scans showed a pseudoaneurysm located proximal to the right subclavian artery. Considering challenges with compression hemostasis and the invasiveness of open surgery, endovascular treatment was selected using a VIABAHN stent graft. A balloon occlusion test of the right vertebral artery was performed to assess stroke risk. Prophylactic embolization of the right vertebral artery, internal thoracic artery, and thyrocervical trunk were performed to prevent a type 2 endoleak. On hospital day 5, our patient showed no postoperative complications and was transferred to the referring hospital. Follow-up imaging showed the graft was intact with no pseudoaneurysm, confirming successful treatment. Endovascular treatment with a stent graft is highly effective for peripheral artery injuries. Using a balloon occlusion test to assess collateral blood flow and stroke risk is essential pretreatment, especially when a graft might occlude the vertebral artery. Balloon occlusion tests are recommended when planning treatment for iatrogenic and other types of subclavian artery injuries.
    Language English
    Publishing date 2024-04-13
    Publishing country Netherlands
    Document type Case Reports
    ZDB-ID 2406300-9
    ISSN 1930-0433
    ISSN 1930-0433
    DOI 10.1016/j.radcr.2024.03.025
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  8. Article ; Online: Glycocalyx analysis of bladder cancer

    Torai Enomoto / Hideshi Okada / Hiroyuki Tomita / Koji Iinuma / Keita Nakane / Yuki Tobisawa / Akira Hara / Takuya Koie

    Frontiers in Cell and Developmental Biology, Vol

    three-dimensional images in electron microscopy and vicia villosa lectin as a marker for invasiveness in frozen sections

    2024  Volume 11

    Abstract: Introduction: The abnormal glycocalyx (GCX) on the surface of cancer cells has been reported to be tall and aberrantly glycosylated and has been linked to the progression and spread of cancer—a finding also observed in bladder cancer. However, the ... ...

    Abstract Introduction: The abnormal glycocalyx (GCX) on the surface of cancer cells has been reported to be tall and aberrantly glycosylated and has been linked to the progression and spread of cancer—a finding also observed in bladder cancer. However, the characteristics of GCX in various types of human bladder cancer remain unknown, and herein, we aimed to provide information on the diversity of glycan components in the GCX of bladder cancers and to shed light on their characteristics.Methods: We used scanning electron microscopy and lanthanum staining to examine the surface GCX of human bladder carcinomas in three-dimensional images, showing the bulky GCX in some carcinomas. We also examined glycan alterations in early to progressive stages of bladder cancers using 20 distinct lectin stains on frozen sections from transurethral resection of bladder tumors.Results and discussion: Distinctive Vicia villosa lectin (VVL) staining was observed in invasive urothelial carcinomas, including those with muscle invasion and variant components. In the clinical setting, cancers with atypia of grades 2–3 had a significantly higher VVL scoring intensity than those with grade 1 atypia (p < 0.005). This study identified that a specific lectin, VVL, was more specific to invasive urothelial carcinomas. This lectin, which selectively binds to sites of cancer progression, is a promising target for drug delivery in future clinical investigations.
    Keywords glycocalyx ; cancer ; lectin ; urinary bladder cancer ; metastasis ; scanning electron microscopy ; Biology (General) ; QH301-705.5
    Subject code 610
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article: Point of tangency between coronavirus disease and endothelial injury.

    Sampei, So / Okada, Hideshi / Tomita, Hiroyuki / Suzuki, Akio / Nawa, Takahide / Ogura, Shinji

    International journal of emergency medicine

    2021  Volume 14, Issue 1, Page(s) 76

    Language English
    Publishing date 2021-12-20
    Publishing country England
    Document type Letter
    ZDB-ID 2411462-5
    ISSN 1865-1380 ; 1865-1372
    ISSN (online) 1865-1380
    ISSN 1865-1372
    DOI 10.1186/s12245-021-00403-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Adjunct Acupuncture Improved Respiratory Status and Weaning from Mechanical Ventilation After Severe COVID-19 Pneumonia.

    Matsumoto-Miyazaki, Jun / Okada, Hideshi / Miura, Tomotaka / Kumada, Keisuke / Naruse, Genki / Miyazaki, Nagisa / Suzuki, Kodai / Yoshida, Shozo / Ogura, Shinji / Okura, Hiroyuki

    Medical acupuncture

    2024  Volume 36, Issue 1, Page(s) 45–52

    Abstract: Background: A patient with severe COVID-19 pneumonia had adjunctive acupuncture to improve respiration and facilitate weaning off prolonged mechanical ventilation (MV).: Case: A man in his 40s with COVID-19 was in an advanced critical-care center on ... ...

    Abstract Background: A patient with severe COVID-19 pneumonia had adjunctive acupuncture to improve respiration and facilitate weaning off prolonged mechanical ventilation (MV).
    Case: A man in his 40s with COVID-19 was in an advanced critical-care center on symptom day 5 for respiratory failure due to pneumonia requiring MV therapy. He received high-dose corticosteroid pulse therapy, antiviral agents, and multiple antibiotics for complicated bacterial pneumonia and bacteremia. Repeated MV weaning attempts failed, although his pneumonia gradually improved. Then, acupuncture 4 times per week was started to improve his respiration and facilitate MV weaning from day 49 of his symptoms' onset.
    Results: His weaning-related indices improved, including reductions in respiratory rate and Rapid Shallow Breath Index. His O
    Conclusions: Acupuncture improved respiration and facilitated MV weaning in a patient with respiratory failure secondary to COVID-19. Adjunctive acupuncture may benefit such patients and others after severe pneumonia. Large cohort studies are needed.
    Language English
    Publishing date 2024-02-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2296110-0
    ISSN 1933-6594 ; 1933-6586
    ISSN (online) 1933-6594
    ISSN 1933-6586
    DOI 10.1089/acu.2023.0064
    Database MEDical Literature Analysis and Retrieval System OnLINE

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