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Article: A Rare Case of Schwannoma of the Right Greater Palatine Nerve in a 17-Year-Old Female Patient.

Stoev, Lyuben / Yankov, Yanko G / Nikolaev, Nikolay I / Stoeva, Martina

2024 Volume 16, Issue 3, Page(s) e56836

Abstract: Schwannomas are not uncommon in the maxillofacial region; however, those with intraoral localization and, in particular, the hard palate are among the least frequently described. In the current case report, we present a 17-year-old girl with a ... ...

Abstract	Schwannomas are not uncommon in the maxillofacial region; however, those with intraoral localization and, in particular, the hard palate are among the least frequently described. In the current case report, we present a 17-year-old girl with a histologically verified schwannoma of the hard palate on the right, originating from the right greater palatine nerve. In her case, despite the lysis of the palatine bone from the tumor compression, the disease is asymptomatic, causing only a weak sensation of local discomfort. The lesion was removed surgically under general anesthesia and the resulting defect of the palatal mucosa was compensated by plastic reconstruction with a lingual mucosal flap on a posterior feeding base. The recovery period was uneventful.
Language	English
Publishing date	2024-03-24
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.56836
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Book ; Online: Chapter Sorption Detoxification as an Addition to Conventional Therapy of Acute Radiation Sickness and Iatrogenic Leukopenia

Sarnatskaya, V. V. / Snezhkova, E. A. / Chekhun, Vasyl F. / Vg, Nikolaev / Bilous, Anatoliy G. / Shevchuk, O. O. / Badakhivska, Kvitoslava I. / Sakhno, L. A.

2019

Keywords	Medical microbiology & virology ; leukopenia, ionizing irradiation, anti-cancer chemotherapy, granulocyte colony stimulating factor, hemosorption, enterosorption, application-sorption therapy
Size	1 Online-Ressource
Publisher	InTechOpen
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021047889
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Effect of Adrenoreceptor Stimulation on Peptidergic Regulation of Cardiac Activity in Newborn Rats.

Iskakov, N G / Anikina, T A / Nikolaev, T I / Krylova, A V / Zefirov, T L

Bulletin of experimental biology and medicine

2023 Volume 176, Issue 1, Page(s) 9–13

Abstract: We studied the effect of adrenoreceptor stimulation on the frequency of spontaneous activity and amplitude-time parameters of isometric contraction of the atrial myocardial strips from newborn rats, as well as the effect of Y receptor stimulation against ...

Abstract	We studied the effect of adrenoreceptor stimulation on the frequency of spontaneous activity and amplitude-time parameters of isometric contraction of the atrial myocardial strips from newborn rats, as well as the effect of Y receptor stimulation against the background of adrenoreceptor activation. After addition of Y
MeSH term(s)	Rats ; Animals ; Neuropeptide Y/pharmacology ; Receptors, Neuropeptide Y/agonists ; Animals, Newborn
Chemical Substances	Neuropeptide Y ; Receptors, Neuropeptide Y
Language	English
Publishing date	2023-12-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	390407-6
ISSN	1573-8221 ; 0007-4888 ; 0365-9615
ISSN (online)	1573-8221
ISSN	0007-4888 ; 0365-9615
DOI	10.1007/s10517-023-05957-1
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Article: The effect of the microelement preparation on the morphobiochemical composition of the blood of calves

S. V. Nikolaev

Agrarnaâ nauka Evro-Severo-Vostoka. 2021 June, v. 22, no. 3

2021

Abstract: ... there was an increase in total protein by 9.6 % (from 54.2 to 59.4 g / l; P<0.05) and an increase ... by 31.9% (from 4.7 * 10 12 to 3.2*10 12) and hemoglobin by 19.5% (from 87.2 to 70.2 g / l; P<0.05 ... with stable values in the experimental group, which were higher by 56.3 % (5.0*1012) and 22.2 % (90.2 g/l ...

Abstract	The paper presents an assessment of the effect of the Sedimine microelement product on the morphobiochemical composition of the blood of calves during the milk feeding period. For the experiment, 2 groups of heifers of HolsteinKholmogory breed at the age of 2-3 weeks were formed. The calves of the experimental group were injected intramuscularly with Sedimine at a dose of 5 ml three times with an interval of 7 days, and the control animals were given a saline solution in the same way. Blood for hematological and biochemical studies was obtained at the beginning of the experiment and a week after the first and last injection. According to the research it has been established, that after a single injection of Sedimine, there were no evident changes in the biochemical profile of the blood, with the exception of a decrease in the level of urea by 30.6% (from 4.9 to 3.4 mmol/l; P<0.001). After three-time administration of the drug, there was an increase in total protein by 9.6 % (from 54.2 to 59.4 g / l; P<0.05) and an increase in AlAT activity by 1.2 times (P<0.001), while in comparison with the control, the activity of AsAT was greater (P<0.01) by 43.4 % (57.5 U/l). The de Ritis coefficient in the experimental group decreased by 43.1 % (to 6.2; P<0.01), while in the control group, on the contrary, the indicator increased by 32.1 % (to 11.1). The ratio of calcium to phosphorus in all animals decreased by 28.8...32.1 % (P<0.001), but in young animals treated with Sedimine, the ratio was 16.7 % (0.84) higher compared to the intact group of calves (P<0.05). The concentration of thyroglobulin in the experimental group significantly decreased by 29.4 % (from 0.34 to 0.24; P<0.01), with stable values in calves that were treated with saline. Injections of the preparation contributed to the stable maintenance of iron in the blood of animals of the experimental group, the concentration of which was 3 times higher (P<0.01) in relation to the control, where the indicator was 5.9 micromol/l. Also, the control animals showed a decrease in the number of red blood cells by 31.9% (from 4.7 * 10 12 to 3.210 12) and hemoglobin by 19.5% (from 87.2 to 70.2 g / l; P<0.05), with stable values in the experimental group, which were higher by 56.3 % (5.01012) and 22.2 % (90.2 g/l) compared to the control (P<0.05). Changes in white blood were characterized by a higher level of white blood cells in calves of the experimental group (by 27.7 %), primarily due to a 36.7 % (4.1*109 Р<0.01) higher number of lymphocytic cells. Thus, a three-time injection of the test drug was quite effective in preventing iron deficiency in calves during milk feeding and had a positive effect on metabolic processes.
Keywords	calcium ; drugs ; hemoglobin ; iron ; milk ; phosphorus ; protein content ; sodium chloride ; thyroglobulin ; urea
Language	English
Dates of publication	2021-06
Size	p. 418-427.
Publishing place	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article
ISSN	2500-1396
DOI	10.30766/2072-9081.2021.22.3.418-427
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The effect of the microelement preparation on the morphobiochemical composition of the blood of calves

S. V. Nikolaev

Аграрная наука Евро-Северо-Востока, Vol 22, Iss 3, Pp 418-

2021 Volume 427

Abstract: ... there was an increase in total protein by 9.6 % (from 54.2 to 59.4 g / l; P<0.05) and an increase ... in the level of urea by 30.6% (from 4.9 to 3.4 mmol/l; P<0.001). After three-time administration of the drug ... greater (P<0.01) by 43.4 % (57.5 U/l). The de Ritis coefficient in the experimental group decreased by 43 ...

Abstract	The paper presents an assessment of the effect of the Sedimine microelement product on the morphobiochemical composition of the blood of calves during the milk feeding period. For the experiment, 2 groups of heifers of HolsteinKholmogory breed at the age of 2-3 weeks were formed. The calves of the experimental group were injected intramuscularly with Sedimine at a dose of 5 ml three times with an interval of 7 days, and the control animals were given a saline solution in the same way. Blood for hematological and biochemical studies was obtained at the beginning of the experiment and a week after the first and last injection. According to the research it has been established, that after a single injection of Sedimine, there were no evident changes in the biochemical profile of the blood, with the exception of a decrease in the level of urea by 30.6% (from 4.9 to 3.4 mmol/l; P<0.001). After three-time administration of the drug, there was an increase in total protein by 9.6 % (from 54.2 to 59.4 g / l; P<0.05) and an increase in AlAT activity by 1.2 times (P<0.001), while in comparison with the control, the activity of AsAT was greater (P<0.01) by 43.4 % (57.5 U/l). The de Ritis coefficient in the experimental group decreased by 43.1 % (to 6.2; P<0.01), while in the control group, on the contrary, the indicator increased by 32.1 % (to 11.1). The ratio of calcium to phosphorus in all animals decreased by 28.8.32.1 % (P<0.001), but in young animals treated with Sedimine, the ratio was 16.7 % (0.84) higher compared to the intact group of calves (P<0.05). The concentration of thyroglobulin in the experimental group significantly decreased by 29.4 % (from 0.34 to 0.24; P<0.01), with stable values in calves that were treated with saline. Injections of the preparation contributed to the stable maintenance of iron in the blood of animals of the experimental group, the concentration of which was 3 times higher (P<0.01) in relation to the control, where the indicator was 5.9 micromol/l. Also, the ...
Keywords	trace elements ; sedimine ; iodine ; selenium ; iron ; metabolism ; calves ; Agriculture ; S
Subject code	630
Language	Russian
Publishing date	2021-06-01T00:00:00Z
Publisher	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Three-dimensional quantitative muscle ultrasound in patients with facioscapulohumeral dystrophy and myotonic dystrophy.

de Jong, Leon / Greco, Anna / Nikolaev, Anton / Weijers, Gert / van Engelen, Baziel G M / de Korte, Chris L / Fütterer, Jurgen J

Muscle & nerve

2023 Volume 68, Issue 4, Page(s) 432–438

Abstract: Introduction/aims: Ultrasound imaging of muscle tissue conventionally results in two-dimensional sampling of tissue. For heterogeneously affected muscles, a sampling error using two-dimensional (2D) ultrasound can therefore be expected. In this study, ... ...

Abstract	Introduction/aims: Ultrasound imaging of muscle tissue conventionally results in two-dimensional sampling of tissue. For heterogeneously affected muscles, a sampling error using two-dimensional (2D) ultrasound can therefore be expected. In this study, we aimed to quantify and extend ultrasound imaging findings in neuromuscular disorders by using three-dimensional quantitative muscle ultrasound (3D QMUS). Methods: Patients with facioscapulohumeral dystrophy (n = 31) and myotonic dystrophy type 1 (n = 16) were included in this study. After physical examination, including Medical Research Council (MRC) scores, the tibialis anterior muscle was scanned with automated ultrasound. QMUS parameters were calculated over 15 cm of the length of the tibialis anterior muscle and were compared with a healthy reference data set. Results: With 3D QMUS local deviations from the healthy reference could be detected. Significant Pearson correlations (P < .01) between MRC score and QMUS parameters in male patients (n = 23) included the mean echo intensity (EI) (0.684), the standard deviation of EI (0.737), and the residual attenuation (0.841). In 91% of all patients, mean EI deviated by more than 1 standard deviation from the healthy reference. In general, the proportion of muscle tissue with a Z score >1 was about 50%. Discussion: In addition to mean EI, multiple QMUS parameters reported in this study are potential biomarkers for pathology. Besides a moderate correlation of mean EI with muscle weakness, two other parameters showed strong correlations: standard deviation of EI and residual attenuation. Local detection of abnormalities makes 3D QMUS a promising method that can be used in research and potentially for clinical evaluation.
MeSH term(s)	Humans ; Male ; Myotonic Dystrophy/diagnostic imaging ; Muscular Dystrophy, Facioscapulohumeral/diagnostic imaging ; Muscle, Skeletal/diagnostic imaging ; Muscle Strength/physiology ; Ultrasonography/methods
Language	English
Publishing date	2023-07-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	438353-9
ISSN	1097-4598 ; 0148-639X
ISSN (online)	1097-4598
ISSN	0148-639X
DOI	10.1002/mus.27943
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Article: Suppression of the Testis-Specific Transcription of the ZBTB32 and ZNF473 Genes in Germ Cell Tumors.

Bulanenkova, S S / Filyukova, O B / Snezhkov, E V / Akopov, S B / Nikolaev, L G

Acta naturae

2022 Volume 14, Issue 3, Page(s) 85–94

Abstract: The family of genes containing C2H2 zinc finger domains, which has more than 700 members, is one of the largest in the genome. Of particular interest are C2H2 genes with potential tissue-specific transcription, which determine the functional properties ... ...

Abstract	The family of genes containing C2H2 zinc finger domains, which has more than 700 members, is one of the largest in the genome. Of particular interest are C2H2 genes with potential tissue-specific transcription, which determine the functional properties of individual cell types, including those associated with pathological processes. The aim of this work was to identify C2H2 family genes with tissue-specific transcription and analyze changes in their activity during tumor progression. To search for these genes, we used four databases containing data on gene transcription in human tissues obtained by RNA-Seq analysis. The analysis showed that, although the major part of the C2H2 family genes is transcribed in virtually all tissues, a group of genes has tissue-specific transcription, with most of the transcripts being found in the testis. After having compared all four databases, we identified nine such genes. The testis-specific transcription was confirmed for two of them, namely ZBTB32 and ZNF473, using quantitative PCR of cDNA samples from different organs. A decrease in ZBTB32 and ZNF473 transcription levels was demonstrated in germ cell tumors. The studied genes can serve as candidate markers in germ cell tumors.
Language	English
Publishing date	2022-09-22
Publishing country	Russia (Federation)
Document type	Journal Article
ISSN	2075-8251
ISSN	2075-8251
DOI	10.32607/actanaturae.11620
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Article ; Online: Сell clusters isolation in glioblastomas and their functional and molecular characterization using new morphometric approaches.

Nikitin, P V / Musina, G R / Fayzullin, A L / Bakulina, A A / Nikolaev, V N / Mikhailov, V P / Werkenbark, L / Kjelin, M / Usachev, D Yu / Timashev, P S

Computers in biology and medicine

2023 Volume 164, Page(s) 107322

Abstract: Background: Digital pathology has come a long way in terms of creating tools to improve existing diagnostic approaches. However, several pathology fields, such as neuropathology, are still characterized by low coverage from machine learning tools and ... ...

Abstract	Background: Digital pathology has come a long way in terms of creating tools to improve existing diagnostic approaches. However, several pathology fields, such as neuropathology, are still characterized by low coverage from machine learning tools and neural network analysis, which may be due to the complexity of the internal cellular and molecular structure of the corresponding neoplasms, including glioblastomas. Method: In the framework of this study, using advanced proprietary tools for obtaining images of histological slides and their deep morphometric analysis, we studied samples of 198 patients with glioblastoma with the selection of morphometric cell clusters. Also, cells of each cluster were isolated, and their proliferative, migratory, invasive activity, survival ability, aerobic glycolysis activity, and chemo- and radioresistance were studied. Results: Four morphometric clusters were identified, including small-cell cluster, paracirculonuclear cluster, hypochromic cluster, and macronuclear cluster, which significantly differed in morphometric parameters and functional parameters. Hypochromic cluster cells demonstrated the highest proliferation activity; macronuclear cluster was the most active glucose consumer; paracirculonuclear cluster had the most prominent migratory and invasive activity and hypoxia resistance; small-cell cluster demonstrated predominantly average values of all parameters. Moreover, additional analysis revealed the presence of a separate subcluster of stem cell elements that correspond in their molecular properties to glioma stem cells and are present in all four clusters. It also turned out that several key molecular parameters of glioblastoma, such as mutational modifications in the EGFR, PDGFRA, and NF1 genes, along with the molecular GBM subtype, are significantly correlated with the identified cell clusters. Conclusions: Thus, the results represent an up-and-coming innovation in the practical field of digital pathology and fundamental questions of glioma carcinogenesis.
MeSH term(s)	Humans ; Glioblastoma/genetics ; Glioblastoma/pathology ; Glioma ; Neural Networks, Computer ; Brain Neoplasms/genetics
Language	English
Publishing date	2023-08-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	127557-4
ISSN	1879-0534 ; 0010-4825
ISSN (online)	1879-0534
ISSN	0010-4825
DOI	10.1016/j.compbiomed.2023.107322
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Article: Efficacy of dispersed fibrous carbon sorbent in treatment of malignant fungating wounds.

Sakhno, L A / Babenko, L P / Lazarenko, L M / Korotych, V G / Sarnatskaya, V V / Snezhkova, E A / Spivak, M Ya / Nikolaev, V G

Experimental oncology

2021 Volume 43, Issue 4, Page(s) 359–364

Abstract: Aim: To evaluate the influence of dispersed fibrous carbon sorbent (DFCS) on malignant fungating wounds (MFWs) in Guerin's carcinoma-bearing rats with an assessment of wound bacterial microflora.: Materials and methods: The study was performed on ... ...

Abstract	Aim: To evaluate the influence of dispersed fibrous carbon sorbent (DFCS) on malignant fungating wounds (MFWs) in Guerin's carcinoma-bearing rats with an assessment of wound bacterial microflora. Materials and methods: The study was performed on female Wistar rats inoculated subcutaneously with Guerin T8 carcinoma into the upper interscapular region. On day 20, the tumors were infected by injecting the suspension of S. aureus 8325-4. After 24 h, gauze dressings were applied daily to the wounds in the control group and DFCS in the experimental one. The state of the wounds was assessed by visual control with photo monitoring, odor control on a verbal rating scale and microbiological analysis of qualitative and quantitative characteristics of wound microbiota. Results: On the 3rd day after the start of dressings, the number of S. aureus of 8325-4 strain was significantly higher in the wounds of rats in control group than experimental one (5.14 ± 0.27 vs 3.43 ± 0.33 lg CFU/ml). The total number of Staphylococci in the DFCS-treated wounds did not differ statistically from the number of S. aureus 8325-4 strain whereas in control ones were higher by an order of magnitude. On the 10th day the total number of Staphylococci and S. aureus 8325-4 in particular, remained consistently high in the wounds of the control rats, while was almost five orders of magnitude lower and represented mainly by S. aureus 8325-4 in the experimental ones. The total number of aerobic and optionally anaerobic microorganisms was significantly lower as well. A greater variety of microorganisms in the gauze-covered wounds, as well as an their increased number were accompanied by enhancement of the wound odor from "noticeable" to "strong". There was an appearance of a "barely noticeable" odor in only one animal from the experimental group. Conclusion: The study has demonstrated the ability of DFCS to control substantially the bacterial microflora as well as malodor of MWFs in vivo. The results obtained can contribute to solving the problem of improving the quality of palliative care for patients with malignant and other chronic wounds.
MeSH term(s)	Animals ; Carbon ; Female ; Humans ; Neoplasms/complications ; Rats ; Rats, Wistar ; Staphylococcus aureus
Chemical Substances	Carbon (7440-44-0)
Language	English
Publishing date	2021-12-29
Publishing country	Ukraine
Document type	Journal Article
ZDB-ID	2176927-8
ISSN	1812-9269 ; 0204-3564
ISSN	1812-9269 ; 0204-3564
DOI	10.32471/exp-oncology.2312-8852.vol-43-no-4.16757
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Article ; Online: PTCH1 inactivation is sufficient to cause basaloid follicular hamartoma in paediatric Nevoid basal cell carcinoma syndrome.

Blanchard, G / Yurchenko, A A / Pop, O T / Weibel, L / Theiler, M / Hauser, V / Fraitag, S / Guenova, E / Flatz, L / Nikolaev, S I / Hohl, D

Journal of the European Academy of Dermatology and Venereology : JEADV

2022 Volume 36, Issue 11, Page(s) e954–e956

MeSH term(s)	Basal Cell Nevus Syndrome/complications ; Carcinoma, Basal Cell/complications ; Carcinoma, Basal Cell/pathology ; Child ; Hamartoma/complications ; Hamartoma/pathology ; Humans ; Skin Neoplasms/complications ; Skin Neoplasms/pathology
Language	English
Publishing date	2022-07-08
Publishing country	England
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	1128828-0
ISSN	1468-3083 ; 0926-9959
ISSN (online)	1468-3083
ISSN	0926-9959
DOI	10.1111/jdv.18382
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