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Article ; Online: Sugar coordinates plant defense signaling.

Yamada, Kohji / Mine, Akira

2024 Volume 10, Issue 4, Page(s) eadk4131

Abstract: Pathogen recognition triggers energy-intensive defense systems. Although successful defense should depend on energy availability, how metabolic information is communicated to defense remains unclear. We show that sugar, especially glucose-6-phosphate ( ... ...

Abstract	Pathogen recognition triggers energy-intensive defense systems. Although successful defense should depend on energy availability, how metabolic information is communicated to defense remains unclear. We show that sugar, especially glucose-6-phosphate (G6P), is critical in coordinating defense in
MeSH term(s)	Sugars ; Signal Transduction ; Anti-Bacterial Agents ; Antifungal Agents ; Arabidopsis ; Chitin
Chemical Substances	Sugars ; Anti-Bacterial Agents ; Antifungal Agents ; Chitin (1398-61-4)
Language	English
Publishing date	2024-01-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2810933-8
ISSN	2375-2548 ; 2375-2548
ISSN (online)	2375-2548
ISSN	2375-2548
DOI	10.1126/sciadv.adk4131
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Direct Observation and Automated Measurement of Stomatal Responses to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana.

Hirata, Rikako / Takagi, Momoko / Toda, Yosuke / Mine, Akira

Journal of visualized experiments : JoVE

2024 , Issue 204

Abstract: Stomata are microscopic pores found in the plant leaf epidermis. Regulation of stomatal aperture is pivotal not only for balancing carbon dioxide uptake for photosynthesis and transpirational water loss but also for restricting bacterial invasion. While ... ...

Abstract	Stomata are microscopic pores found in the plant leaf epidermis. Regulation of stomatal aperture is pivotal not only for balancing carbon dioxide uptake for photosynthesis and transpirational water loss but also for restricting bacterial invasion. While plants close stomata upon recognition of microbes, pathogenic bacteria, such as Pseudomonas syringae pv. tomato DC3000 (Pto), reopen the closed stomata to gain access into the leaf interior. In conventional assays for assessing stomatal responses to bacterial invasion, leaf epidermal peels, leaf discs, or detached leaves are floated on bacterial suspension, and then stomata are observed under a microscope followed by manual measurement of stomatal aperture. However, these assays are cumbersome and may not reflect stomatal responses to natural bacterial invasion in a leaf attached to the plant. Recently, a portable imaging device was developed that can observe stomata by pinching a leaf without detaching it from the plant, together with a deep learning-based image analysis pipeline designed to automatically measure stomatal aperture from leaf images captured by the device. Here, building on these technical advances, a new method to assess stomatal responses to bacterial invasion in Arabidopsis thaliana is introduced. This method consists of three simple steps: spray inoculation of Pto mimicking natural infection processes, direct observation of stomata on a leaf of the Pto-inoculated plant using the portable imaging device, and automated measurement of stomatal aperture by the image analysis pipeline. This method was successfully used to demonstrate stomatal closure and reopening during Pto invasion under conditions that closely mimic the natural plant-bacteria interaction.
MeSH term(s)	Arabidopsis ; Pseudomonas syringae ; Solanum lycopersicum ; Biological Assay ; Biological Transport
Language	English
Publishing date	2024-02-09
Publishing country	United States
Document type	Journal Article ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/66112
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Article: Structure and dynamics of the plant immune signaling network in plant–bacteria interactions

Mine, Akira

Journal of general plant pathology. 2020 Nov., v. 86, no. 6

2020

Abstract: The plant innate immune system deploys receptor proteins on the cell surface or inside the cell to sense microbial invasions. Perception of microbe associated molecular patterns (MAMPs), which are typically derived from conserved molecules in a class of ... ...

Abstract	The plant innate immune system deploys receptor proteins on the cell surface or inside the cell to sense microbial invasions. Perception of microbe associated molecular patterns (MAMPs), which are typically derived from conserved molecules in a class of microbes such as bacterial flagellin, triggers pattern-triggered immunity (PTI). While pathogens possess a repertoire of effectors that dampen PTI, plants detect the presence or actions of effectors and induce effector-triggered immunity (ETI) to prevent pathogen infection. PTI and ETI utilize a number of common signaling components such as reactive oxygen species, mitogen-activated protein kinases (MAPKs), and phytohormones to reprogram the cell state toward defense responses. It has become increasingly clear that these signaling components do not act in isolation but interact with each other to form an intricate network. Here, I discuss structure and dynamics of the plant immune signaling network that operate behind the interaction between the model plant Arabidopsis thaliana and the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pto).
Keywords	Arabidopsis thaliana ; Pseudomonas syringae pv. tomato ; flagellin ; innate immunity ; mitogen-activated protein kinase ; pathogens ; plant hormones ; plant pathology ; reactive oxygen species
Language	English
Dates of publication	2020-11
Size	p. 528-530.
Publishing place	Springer Japan
Document type	Article
Note	NAL-AP-2-clean ; Review
ZDB-ID	2016032-X
ISSN	1345-2630
ISSN	1345-2630
DOI	10.1007/s10327-020-00954-y
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Commensal lifestyle regulated by a negative feedback loop between Arabidopsis ROS and the bacterial T2SS.

Entila, Frederickson / Han, Xiaowei / Mine, Akira / Schulze-Lefert, Paul / Tsuda, Kenichi

Nature communications

2024 Volume 15, Issue 1, Page(s) 456

Abstract: Despite the plant health-promoting effects of plant microbiota, these assemblages also comprise potentially detrimental microbes. How plant immunity controls its microbiota to promote plant health under these conditions remains largely unknown. We find ... ...

Abstract	Despite the plant health-promoting effects of plant microbiota, these assemblages also comprise potentially detrimental microbes. How plant immunity controls its microbiota to promote plant health under these conditions remains largely unknown. We find that commensal bacteria isolated from healthy Arabidopsis plants trigger diverse patterns of reactive oxygen species (ROS) production dependent on the immune receptors and completely on the NADPH oxidase RBOHD that selectively inhibited specific commensals, notably Xanthomonas L148. Through random mutagenesis, we find that L148 gspE, encoding a type II secretion system (T2SS) component, is required for the damaging effects of Xanthomonas L148 on rbohD mutant plants. In planta bacterial transcriptomics reveals that RBOHD suppresses most T2SS gene expression including gspE. L148 colonization protected plants against a bacterial pathogen, when gspE was inhibited by ROS or mutation. Thus, a negative feedback loop between Arabidopsis ROS and the bacterial T2SS tames a potentially detrimental leaf commensal and turns it into a microbe beneficial to the host.
MeSH term(s)	Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Reactive Oxygen Species/metabolism ; Feedback ; NADPH Oxidases/genetics ; NADPH Oxidases/metabolism ; Bacteria/metabolism ; Gene Expression Regulation, Plant ; Plant Immunity/genetics
Chemical Substances	Arabidopsis Proteins ; Reactive Oxygen Species ; NADPH Oxidases (EC 1.6.3.-)
Language	English
Publishing date	2024-01-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-44724-2
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Article ; Online: Commensal lifestyle regulated by a negative feedback loop between Arabidopsis ROS and the bacterial T2SS

Frederickson Entila / Xiaowei Han / Akira Mine / Paul Schulze-Lefert / Kenichi Tsuda

Nature Communications, Vol 15, Iss 1, Pp 1-

2024 Volume 17

Abstract: Abstract Despite the plant health-promoting effects of plant microbiota, these assemblages also comprise potentially detrimental microbes. How plant immunity controls its microbiota to promote plant health under these conditions remains largely unknown. ... ...

Abstract	Abstract Despite the plant health-promoting effects of plant microbiota, these assemblages also comprise potentially detrimental microbes. How plant immunity controls its microbiota to promote plant health under these conditions remains largely unknown. We find that commensal bacteria isolated from healthy Arabidopsis plants trigger diverse patterns of reactive oxygen species (ROS) production dependent on the immune receptors and completely on the NADPH oxidase RBOHD that selectively inhibited specific commensals, notably Xanthomonas L148. Through random mutagenesis, we find that L148 gspE, encoding a type II secretion system (T2SS) component, is required for the damaging effects of Xanthomonas L148 on rbohD mutant plants. In planta bacterial transcriptomics reveals that RBOHD suppresses most T2SS gene expression including gspE. L148 colonization protected plants against a bacterial pathogen, when gspE was inhibited by ROS or mutation. Thus, a negative feedback loop between Arabidopsis ROS and the bacterial T2SS tames a potentially detrimental leaf commensal and turns it into a microbe beneficial to the host.
Keywords	Science ; Q
Subject code	580
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Age-related appearance of lamellar structures in lens capsule of cataractous eyes and its pathological significance.

Hirata, Akira / Mine, Keiko / Hayashi, Ken

Journal of cataract and refractive surgery

2022 Volume 48, Issue 7, Page(s) 844–849

Abstract: Purpose: To determine morphological changes in the lens capsule with aging.: Setting: Hayashi Eye Hospital, Fukuoka, Japan.: Design: Cross-sectional study.: Methods: 25 eyes from the older patient group (aged 80 years or older) and 25 eyes from ...

Abstract	Purpose: To determine morphological changes in the lens capsule with aging. Setting: Hayashi Eye Hospital, Fukuoka, Japan. Design: Cross-sectional study. Methods: 25 eyes from the older patient group (aged 80 years or older) and 25 eyes from the younger patient group (aged 65 years or younger) who were diagnosed with cataract and indicated for surgery were included in the study. After continuous curvilinear capsulorhexis, the anterior lens capsule was collected, immediately fixed, and processed for electron microscopy analysis. Backscattered electron images of the cross-section of the anterior lens capsule were observed under a scanning electron microscope. The ultrastructure of the anterior lens capsule was observed and compared between the groups. Factors associated with the occurrence of the lamellar structure were also identified, with the presence or absence of a lamellar structure as an objective variable and preoperative clinical characteristics as the explanatory variables. Results: 50 eyes of 50 patients were included. In the younger patient group, 20 eyes (80%) had a homogeneous lens capsule, whereas 5 eyes had lamellar structures. By contrast, in the older patient group, 5 eyes had homogeneous structures, while the remaining 20 eyes (80%) had lamellar structures. 1 eye showed capsular delamination. The only significant factor for the occurrence of lamellar structures was age group ( P < .01, nominal logistic regression analysis). Conclusions: Lamellar structures appear in the anterior capsule during aging. The appearance of lamellar structures indicates fragility of the lens capsule, which may, in turn, lead to capsular delamination or lens dislocation in some cases.
MeSH term(s)	Anterior Capsule of the Lens/pathology ; Capsulorhexis/methods ; Cataract/complications ; Cross-Sectional Studies ; Humans ; Lens Capsule, Crystalline/surgery
Language	English
Publishing date	2022-03-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632744-8
ISSN	1873-4502 ; 0886-3350
ISSN (online)	1873-4502
ISSN	0886-3350
DOI	10.1097/j.jcrs.0000000000000967
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Article ; Online: Contractility of temporal inverted internal limiting membrane flap after vitrectomy for macular hole.

Hirata, Akira / Mine, Keiko / Hayashi, Ken

Scientific reports

2021 Volume 11, Issue 1, Page(s) 20035

Abstract: We investigated the postoperative visual outcomes and morphological changes of the internal limiting membrane (ILM) flap, in patients who underwent the temporal inverted ILM flap technique for macular hole (MH). Between August 2018 and February 2020, 22 ... ...

Abstract	We investigated the postoperative visual outcomes and morphological changes of the internal limiting membrane (ILM) flap, in patients who underwent the temporal inverted ILM flap technique for macular hole (MH). Between August 2018 and February 2020, 22 eyes of 22 patients with idiopathic or myopic MH who underwent vitrectomy with ILM flap were included in this study and followed-up for more than 6 months. Postoperative MH status, comparison of best-corrected visual acuity (BCVA) before and 6 months after surgery, changes in the ILM flap area at 1 and 6 months postoperatively, and the factors related to changes in ILM flap size, were analyzed. MH closure was achieved in all of the patients. The BCVA at 6 months postoperatively (0.18 ± 0.15) was significantly better than the preoperative BCVA of 0.63 ± 0.37 (P < 0.001, paired t test). The area of the ILM flap decreased significantly from 3.25 ± 1.27 mm
MeSH term(s)	Aged ; Basement Membrane/pathology ; Basement Membrane/surgery ; Epiretinal Membrane/pathology ; Epiretinal Membrane/surgery ; Female ; Humans ; Male ; Middle Aged ; Myopia/complications ; Prospective Studies ; Reoperation ; Retinal Detachment/etiology ; Retinal Detachment/pathology ; Retinal Detachment/surgery ; Retinal Perforations/etiology ; Retinal Perforations/pathology ; Retinal Perforations/surgery ; Surgical Flaps/pathology ; Surgical Flaps/surgery ; Visual Acuity ; Vitrectomy/adverse effects
Language	English
Publishing date	2021-10-08
Publishing country	England
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-99509-0
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Article ; Online: Two stages of salvaging an extensively necrotic foot with chronic limb-threatening ischemia by arterialization of great saphenous vein and free latissimus dorsi musculocutaneous flap transfer for wound coverage with the arterialized vein as the recipient vessel: A case report.

Kagaya, Yu / Ohura, Norihiko / Miyamoto, Akira / Ozaki, Mine

Microsurgery

2022 Volume 43, Issue 2, Page(s) 166–170

Abstract: Patients with chronic limb-threatening ischemia (CLTI) without other options for adequate arterial revascularization could undergo deep (or distal) venous arterialization for limb salvage. Additionally, patients with extensive foot wound with CLTI ... ...

Abstract	Patients with chronic limb-threatening ischemia (CLTI) without other options for adequate arterial revascularization could undergo deep (or distal) venous arterialization for limb salvage. Additionally, patients with extensive foot wound with CLTI sometimes require free flap transfer for limb salvage. We herein report a case of successful reconstructive limb-salvage surgery for an extensively necrotic foot with CLTI, using a two-stage operation involving venous arterialization using foot-perforating veins and subsequent free flap transfer (with preservation of the arterialized vein). The patient was a 59-year-old man with CLTI. The patient's right foot had dry necrotic tissue after Lisfranc joint amputation. Only one straight-line to the posterior tibial artery was achieved after endovascular therapies (four times). At the first stage of surgery, an arterial-venous shunt bypass from the superficial femoral artery to the distal great saphenous vein (GSV) (near the foot-perforating vein) was created. Arterial blood supply reached the necrotic area via the foot venous circulation system. At the second stage of surgery, free latissimus dorsi musculocutaneous flap (8 × 27 cm) transfer with preservation of the arterialized vein was performed. The pedicle artery was anastomosed to the bypass graft (end-to-side). The pedicle vein was anastomosed to the proximal stump of the GSV (end-to-end). The flap and residual foot survived completely, at a one-year follow-up postoperatively. An indocyanine green bypass-through angiography revealed the angiosome through the venous arterialization bypass graft, which included the flap; entire forefoot; and partial regions of the midfoot and heel. This two-stage operation might be considered a useful option for limb-salvage and complete wound-healing in patients with severe non-healing wound with CLTI. The two methods could compensate and overcome the problems of either method: incomplete wound-healing after venous arterialization, and the absence of a recipient artery for free flap transfer.
MeSH term(s)	Male ; Humans ; Middle Aged ; Saphenous Vein/transplantation ; Chronic Limb-Threatening Ischemia ; Myocutaneous Flap/surgery ; Superficial Back Muscles/transplantation ; Treatment Outcome ; Limb Salvage/methods ; Ischemia/surgery ; Peripheral Arterial Disease/surgery
Language	English
Publishing date	2022-12-22
Publishing country	United States
Document type	Case Reports
ZDB-ID	605524-2
ISSN	1098-2752 ; 0738-1085
ISSN (online)	1098-2752
ISSN	0738-1085
DOI	10.1002/micr.31001
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Article ; Online: Contractility of temporal inverted internal limiting membrane flap after vitrectomy for macular hole

Akira Hirata / Keiko Mine / Ken Hayashi

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 10

Abstract: Abstract We investigated the postoperative visual outcomes and morphological changes of the internal limiting membrane (ILM) flap, in patients who underwent the temporal inverted ILM flap technique for macular hole (MH). Between August 2018 and February ... ...

Abstract	Abstract We investigated the postoperative visual outcomes and morphological changes of the internal limiting membrane (ILM) flap, in patients who underwent the temporal inverted ILM flap technique for macular hole (MH). Between August 2018 and February 2020, 22 eyes of 22 patients with idiopathic or myopic MH who underwent vitrectomy with ILM flap were included in this study and followed-up for more than 6 months. Postoperative MH status, comparison of best-corrected visual acuity (BCVA) before and 6 months after surgery, changes in the ILM flap area at 1 and 6 months postoperatively, and the factors related to changes in ILM flap size, were analyzed. MH closure was achieved in all of the patients. The BCVA at 6 months postoperatively (0.18 ± 0.15) was significantly better than the preoperative BCVA of 0.63 ± 0.37 (P < 0.001, paired t test). The area of the ILM flap decreased significantly from 3.25 ± 1.27 mm2 at 1 month to 3.13 ± 1.23 mm2 at 6 months (P = 0.024, Wilcoxon signed-rank test). Two eyes showed an ILM flap contraction of more than 20%, and one eye required reoperation due to an increase in metamorphopsia and decreased visual acuity. Among age, sex, ILM flap area at 1 month, preoperative BCVA, and axial length, ILM flap contraction was correlated with patient age and ILM flap area. Although vitrectomy with the inverted ILM flap technique confers a good visual outcome, the ILM flap may contract in younger patients.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: General anesthesia for cesarean section in a pregnant woman with systemic vascular malformation: a case report.

Takeuchi, Noriko / Koshihara, Misa / Motoyasu, Akira / Tokumine, Joho / Nakazawa, Harumasa / Ozaki, Mine / Yorozu, Tomoko

JA clinical reports

2023 Volume 9, Issue 1, Page(s) 88

Abstract: Background: Vascular malformations are composed of morphologically abnormal vascular tissue, and when located in the head and neck region, they can make it difficult to secure the airway during general anesthesia.: Case presentation: A 28-year-old ... ...

Abstract	Background: Vascular malformations are composed of morphologically abnormal vascular tissue, and when located in the head and neck region, they can make it difficult to secure the airway during general anesthesia. Case presentation: A 28-year-old pregnant woman with vascular malformations in the pharynx was scheduled to undergo a cesarean section, for which spinal anesthesia was initially chosen. However, after magnetic resonance imaging results revealed the presence of multiple vascular malformations in the lumbar multifidus muscles, spinal anesthesia was considered to be of high risk. Thus, the patient was subjected to general anesthesia tracheal intubation under sedation, and the course of the surgery was without complications. Conclusions: Because the pathophysiology and clinical sequelae of vascular malformations may be involved in complications, thorough presurgical evaluation of the patient's physical condition and careful anesthesia planning should be done.
Language	English
Publishing date	2023-12-14
Publishing country	Germany
Document type	Journal Article
ISSN	2363-9024
ISSN (online)	2363-9024
DOI	10.1186/s40981-023-00682-0
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