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  1. Article: An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM).

    Ali, Rizwan / El-Boubbou, Kheireddine / Boudjelal, Mohamed

    MethodsX

    2021  Volume 8, Page(s) 101521

    Abstract: Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with ... ...

    Abstract Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging.•An easy and rapid method to visualize biological specimens under SEM.•Cells are grown on carbon tapes and gold coated.•Air drying without compromising the image quality.
    Language English
    Publishing date 2021-09-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2830212-6
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2021.101521
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Why do SARS-COV vaccines not exist? The pharma scientific intelligence and business model must be revisited!

    Boudjelal, Mohamed / Nehdi, Atef / Islam, Imadul

    Expert opinion on drug discovery

    2020  Volume 15, Issue 11, Page(s) 1233–1235

    MeSH term(s) Betacoronavirus/immunology ; COVID-19 ; COVID-19 Vaccines ; Coronavirus Infections/immunology ; Coronavirus Infections/prevention & control ; Drug Development ; Drug Discovery ; Drug Industry/standards ; Drug Industry/trends ; Humans ; Pandemics/prevention & control ; Pneumonia, Viral/prevention & control ; SARS-CoV-2 ; Viral Vaccines/immunology ; Viral Vaccines/standards ; Viral Vaccines/supply & distribution
    Chemical Substances COVID-19 Vaccines ; Viral Vaccines
    Keywords covid19
    Language English
    Publishing date 2020-06-16
    Publishing country England
    Document type Editorial
    ZDB-ID 2259618-5
    ISSN 1746-045X ; 1746-0441
    ISSN (online) 1746-045X
    ISSN 1746-0441
    DOI 10.1080/17460441.2020.1777980
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6388: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Caring for patients with rare diseases during the COVID-19 pandemic.

    Boudjelal, Mohamed / Mehyar, Nimer / Alghanem, Bandar / Almajed, Faisal / Alfadhel, Majid

    Journal of infection in developing countries

    2021  Volume 15, Issue 4, Page(s) 450–462

    Abstract: Rare diseases frequently attack and weaken the immune system, increasing the patient's vulnerability to develop severe conditions after viral infections, such as COVID-19. Many patients with rare diseases also suffer from mental retardation and ... ...

    Abstract Rare diseases frequently attack and weaken the immune system, increasing the patient's vulnerability to develop severe conditions after viral infections, such as COVID-19. Many patients with rare diseases also suffer from mental retardation and disability. These rare disease phenotypes do not emerge in older people who are susceptible to COVID-19 infection, but present at a very young age or at birth. These factors must be taken in consideration when caring for this vulnerable patient population during a pandemic, such as COVID-19. Patients with a rare disease have to take their regular medication continuously to control their condition and frequently, the medications, directly or indirectly, affect their immune system. It is important for this patient population, if infected with COVID-19 or another severe form of infection, to adjust the treatment protocol by specialists, in consultation with their own medical team. Special awareness and educational programs, understandable for mentally retarded patients, must be developed to educate them about social distancing, curfew, sanitization, and sensitization to the disease and quarantine. The COVID-19 pandemic highlighted the importance to reconsider the care required by patients with a rare disease during a pandemic or disaster, a program that should be adopted by the World Health Organization and governmental institutions for consideration.
    MeSH term(s) COVID-19/drug therapy ; COVID-19/epidemiology ; COVID-19/etiology ; COVID-19/therapy ; COVID-19 Vaccines/pharmacology ; Cardiovascular Diseases/etiology ; Cytokine Release Syndrome/etiology ; Humans ; Patient Care/psychology ; Rare Diseases/etiology ; Rare Diseases/therapy
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2021-04-30
    Publishing country Italy
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2394024-4
    ISSN 1972-2680 ; 2036-6590
    ISSN (online) 1972-2680
    ISSN 2036-6590
    DOI 10.3855/jidc.13214
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)

    Rizwan Ali / Kheireddine El-Boubbou / Mohamed Boudjelal

    MethodsX, Vol 8, Iss , Pp 101521- (2021)

    2021  

    Abstract: Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with ... ...

    Abstract Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging. • An easy and rapid method to visualize biological specimens under SEM. • Cells are grown on carbon tapes and gold coated. • Air drying without compromising the image quality.
    Keywords Scanning electron microscopy ; Cancer cells ; SEM preparation ; HDMS ; Biological samples ; Science ; Q
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Angiotensin II modulates THP-1-like macrophage phenotype and inflammatory signatures via angiotensin II type 1 receptor.

    Barhoumi, Tlili / Mansour, Fatmah A / Jalouli, Maroua / Alamri, Hassan S / Ali, Rizwan / Harrath, Abdel Halim / Aljumaa, Maha / Boudjelal, Mohamed

    Frontiers in cardiovascular medicine

    2023  Volume 10, Page(s) 1129704

    Abstract: Angiotensin II (Ang II) is a major component of the renin-angiotensin or renin-angiotensin-aldosterone system, which is the main element found to be involved in cardiopathology. Recently, long-term metabolomics studies have linked high levels of ... ...

    Abstract Angiotensin II (Ang II) is a major component of the renin-angiotensin or renin-angiotensin-aldosterone system, which is the main element found to be involved in cardiopathology. Recently, long-term metabolomics studies have linked high levels of angiotensin plasma to inflammatory conditions such as coronary heart disease, obesity, and type 2 diabetes. Monocyte/macrophage cellular function and phenotype orchestrate the inflammatory response in various pathological conditions, most notably cardiometabolic disease. An activation of the Ang II system is usually associated with inflammation and cardiovascular disease; however, the direct effect on monocyte/macrophages has still not been well elucidated. Herein, we have evaluated the cellular effects of Ang II on THP-1-derived macrophages. Ang II stimulated the expression of markers involved in monocyte/macrophage cell differentiation (e.g., CD116), as well as adhesion, cell-cell interaction, chemotaxis, and phagocytosis (CD15, CD44, CD33, and CD49F). Yet, Ang II increased the expression of proinflammatory markers (HLA-DR, TNF-α, CD64, CD11c, and CD38) and decreased CD206 (mannose receptor), an M2 marker. Moreover, Ang II induced cytosolic calcium overload, increased reactive oxygen species, and arrested cells in the G1 phase. Most of these effects were induced via the angiotensin II type 1 receptor (AT1R). Collectively, our results provide new evidence in support of the effect of Ang II in inflammation associated with cardiometabolic diseases.
    Language English
    Publishing date 2023-08-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2781496-8
    ISSN 2297-055X
    ISSN 2297-055X
    DOI 10.3389/fcvm.2023.1129704
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Author Correction: Integration of in vitro and in-silico analysis of Caulerpa racemosa against antioxidant, antidiabetic, and anticancer activities.

    Dissanayake, Indeewarie H / Bandaranayake, Upeka / Keerthirathna, Lakshika R / Manawadu, Chamalika / Silva, Rajitha M / Mohamed, Boudjelal / Ali, Rizwan / Peiris, Dinithi C

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 3962

    Language English
    Publishing date 2023-03-09
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-30967-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: An easy, fast and inexpensive method of preparing a biological specimen for scanning electron microscopy (SEM)

    Nadwī, Riḍwān ʻAlī / El-Boubbou, Kheireddine / Boudjelal, Mohamed

    MethodsX. 2021, v. 8 p.101521-

    2021  

    Abstract: Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with ... ...

    Abstract Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging.•An easy and rapid method to visualize biological specimens under SEM.•Cells are grown on carbon tapes and gold coated.•Air drying without compromising the image quality.
    Keywords air ; breast neoplasms ; carbon ; cell culture ; electron microscopy ; gold ; neoplasm cells ; rapid methods ; Fast and Easy method of preparing biological specimen for SEM ; Scanning electron microscopy ; Cancer cells ; SEM preparation ; HDMS ; Biological samples
    Language English
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 2830212-6
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2021.101521
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: Stimulatory effects of

    Alghamdi, Khalid / Alehaideb, Zeyad / Kumar, Ashok / Al-Eidi, Hamad / Alghamdi, Sahar S / Suliman, Rasha / Ali, Rizwan / Almourfi, Feras / Alghamdi, Saleh M / Boudjelal, Mohamed / Matou-Nasri, Sabine

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1169812

    Abstract: There is no first-line treatment for vitiligo, a skin disease characterized by a lack of melanin produced by the melanocytes, resulting in an urgent demand for new therapeutic drugs capable of stimulating melanocyte functions, including melanogenesis. In ...

    Abstract There is no first-line treatment for vitiligo, a skin disease characterized by a lack of melanin produced by the melanocytes, resulting in an urgent demand for new therapeutic drugs capable of stimulating melanocyte functions, including melanogenesis. In this study, traditional medicinal plant extracts were tested for cultured human melanocyte proliferation, migration, and melanogenesis using MTT, scratch wound-healing assays, transmission electron microscopy, immunofluorescence staining, and Western blot technology. Of the methanolic extracts,
    Language English
    Publishing date 2023-04-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1169812
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.

    Rashid, Mamoon / Ali, Rizwan / Almuzzaini, Bader / Song, Hao / AlHallaj, Alshaimaa / Abdulkarim, Al Abdulrahman / Mohamed Baz, Omar / Al Zahrani, Hajar / Mustafa Sabeena, Muhammed / Alharbi, Wardah / Hussein, Mohamed / Boudjelal, Mohamed

    Cancer medicine

    2021  Volume 10, Issue 22, Page(s) 8138–8150

    Abstract: The colony stimulating factor 2 receptor subunit beta (CSF2RB) is the common signaling subunit of the cytokine receptors for IL-3, IL-5, and GM-CSF. Several studies have shown that spontaneous and random mutants of CSF2RB can lead to ligand independence ... ...

    Abstract The colony stimulating factor 2 receptor subunit beta (CSF2RB) is the common signaling subunit of the cytokine receptors for IL-3, IL-5, and GM-CSF. Several studies have shown that spontaneous and random mutants of CSF2RB can lead to ligand independence in vitro. To date, no report(s) have been shown for the presence of potentially transforming and oncogenic CSF2RB mutation(s) clinically in cancer patients until the first reported case of a leukemia patient in 2016 harboring a germline-activating mutation (R461C). We combined exome sequencing, pathway analyses, and functional assays to identify novel somatic mutations in KAIMRC1 cells and breast tumor specimen. The patient's peripheral blood mononuclear cell (PBMC) exome served as a germline control in the identification of somatic mutations. Here, we report the discovery of a novel potentially transforming and oncogenic somatic mutation (S230I) in the CSF2RB gene of a breast cancer patient and the cell line, KAIMRC1 established from her breast tumor tissue. KAIMRC1 cells are immortalized and shown to survive and proliferate in ligand starvation condition. Immunoblot analysis showed that mutant CSF2RB signals through JAK2/STAT and PI3K/mTOR pathways in ligand starvation conditions. Screening a small molecule kinase inhibitor library revealed potent JAK2 inhibitors against KAIMRC1 cells. We, for the first time, identified a somatic, potentially transforming, and oncogenic CSF2RB mutation (S230I) in breast cancer patients that seem to be an actionable mutation leading to the development of new therapeutics for breast cancer.
    MeSH term(s) Breast Neoplasms/genetics ; Cell Line, Tumor ; Cell Proliferation ; Cytokine Receptor Common beta Subunit/metabolism ; Female ; Germ-Line Mutation ; Humans
    Chemical Substances CSF2RB protein, human ; Cytokine Receptor Common beta Subunit
    Language English
    Publishing date 2021-11-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2659751-2
    ISSN 2045-7634 ; 2045-7634
    ISSN (online) 2045-7634
    ISSN 2045-7634
    DOI 10.1002/cam4.4106
    Database MEDical Literature Analysis and Retrieval System OnLINE

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