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Article ; Online: Angiotensin II hypertension along the female rat tubule: predicted impact on coupled transport of Na

Edwards, Aurélie / Ralph, Donna L / Mercado, Adriana / McDonough, Alicia A

American journal of physiology. Renal physiology

2023 Volume 325, Issue 6, Page(s) F733–F749

Abstract: Chronic infusion of subpressor level of angiotensin II (ANG II) increases the abundance of ... ...

Abstract	Chronic infusion of subpressor level of angiotensin II (ANG II) increases the abundance of Na
MeSH term(s)	Female ; Animals ; Rats ; Kidney Tubules/physiopathology ; Hypertension/physiopathology ; Angiotensin II/pharmacology ; Sodium/metabolism ; Potassium/metabolism ; Rats, Sprague-Dawley ; Computer Simulation ; Male ; Symporters/metabolism
Chemical Substances	Angiotensin II (11128-99-7) ; Sodium (9NEZ333N27) ; Potassium (RWP5GA015D) ; Symporters
Language	English
Publishing date	2023-10-12
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	603837-2
ISSN	1522-1466 ; 0363-6127
ISSN (online)	1522-1466
ISSN	0363-6127
DOI	10.1152/ajprenal.00232.2023
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Article ; Online: Potential of Polyphenols to Restore SIRT1 and NAD+ Metabolism in Renal Disease.

Tovar-Palacio, Claudia / Noriega, Lilia G / Mercado, Adriana

Nutrients

2022 Volume 14, Issue 3

Abstract: SIRT1 is an ... ...

Abstract	SIRT1 is an NAD
MeSH term(s)	Humans ; Kidney/metabolism ; Kidney Diseases/drug therapy ; Kidney Diseases/metabolism ; NAD/metabolism ; Polyphenols/pharmacology ; Sirtuin 1/metabolism
Chemical Substances	Polyphenols ; NAD (0U46U6E8UK) ; SIRT1 protein, human (EC 3.5.1.-) ; Sirtuin 1 (EC 3.5.1.-)
Language	English
Publishing date	2022-02-03
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu14030653
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Article ; Online: Antimicrobial characterization of a titanium coating derived from mussel-glue and Bothrops asper snake venom for the prevention of implant-associated infections caused by Staphylococcus

Adriana Gauna / Luis Mercado / Fanny Guzmán

Electronic Journal of Biotechnology, Vol 56, Iss , Pp 31-

2022 Volume 38

Abstract: ... of the cases of orthopedic implant infection.How to cite: Gauna A, Mercado L, Guzmán F. Antimicrobial ...

Abstract	Background: Proliferation of bacteria, such as Staphylococcus aureus and Staphylococcus epidermidis, on orthopedic implants has been a challenge in orthopedic surgery, highlighting the problem of the increasing antibiotic resistance and the necessity to develop new antimicrobial agents. In this sense, antimicrobial peptides are promising candidates, which can be attached to titanium surfaces in order to make them safer. Mytilus mussels are characterized by adhering efficiently to a wide variety of surfaces, especially metallic ones, through adhesive proteins with a high content of dihydroxyphenylalanine, which is a post-translational modification of tyrosine. Results: This work refers to the synthesis of a coating based on a bifunctional peptide that combines a sequence derived from the mussel foot protein-5 and the antimicrobial peptide pEM-2, derived from Bothrops asper snake venom. The adhesive properties of this bifunctional peptide were evaluated, as well as the adhesive sequence without the pEM-2, using a Quartz Crystal Microbalance. The results showed that the presence of the antimicrobial peptide improved the adhesion; however, a loss of the bactericidal activity was observed. Even so, the adhesive sequence by itself exhibited an important antifouling activity, preventing S. aureus and S. epidermidis adhesion to titanium by 75% and 45%, respectively, although the result against S. epidermidis was not significant. Conclusions: A 13-residue peptide derived from a natural and biocompatible source, like a Mytilus mussel adhesive protein, could be projected as a protective agent on titanium surfaces against S. aureus and S. epidermidis, being responsible for two thirds of the cases of orthopedic implant infection.How to cite: Gauna A, Mercado L, Guzmán F. Antimicrobial characterization of a titanium coating derived from mussel-glue and Bothrops asper snake venom for the prevention of implant-associated infections caused by Staphylococcus. Electron J Biotechnol 2022;56. ...
Keywords	Antifouling coating ; Antimicrobial peptides ; Bothrops asper snake venom ; Implant-associated infection ; Mussel coating ; Mussel-glue ; Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5
Subject code	616
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: SLC12A cryo-EM: analysis of relevant ion binding sites, structural domains, and amino acids.

Trejo, Fátima / Elizalde, Stephanie / Mercado, Adriana / Gamba, Gerardo / de losHeros, Paola

American journal of physiology. Cell physiology

2023 Volume 325, Issue 4, Page(s) C921–C939

Abstract: The solute carrier family 12A (SLC12A) superfamily of membrane transporters modulates the movement of cations coupled with chloride across the membrane. In doing so, these cotransporters are involved in numerous aspects of human physiology: cell volume ... ...

Abstract	The solute carrier family 12A (SLC12A) superfamily of membrane transporters modulates the movement of cations coupled with chloride across the membrane. In doing so, these cotransporters are involved in numerous aspects of human physiology: cell volume regulation, ion homeostasis, blood pressure regulation, and neurological action potential via intracellular chloride concentration modulation. Their physiological characterization has been largely studied; however, understanding the mechanics of their function and the relevance of structural domains or specific amino acids has been a pending task. In recent years, single-particle cryogenic electron microscopy (cryo-EM) has been successfully applied to members of the SLC12A family including all K
MeSH term(s)	Humans ; Cryoelectron Microscopy ; Amino Acids ; Chlorides/metabolism ; Sodium/metabolism ; Cations ; Binding Sites
Chemical Substances	Amino Acids ; Chlorides ; Sodium (9NEZ333N27) ; Cations
Language	English
Publishing date	2023-08-07
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	392098-7
ISSN	1522-1563 ; 0363-6143
ISSN (online)	1522-1563
ISSN	0363-6143
DOI	10.1152/ajpcell.00089.2023
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Article: Antimicrobial characterization of a titanium coating derived from mussel-glue and Bothrops asper snake venom for the prevention of implant-associated infections caused by Staphylococcus

Gauna, Adriana / Mercado, Luis / Guzmán, Fanny

Electronic Journal of Biotechnology. 2022 Mar., v. 56

2022

Abstract: ... of orthopedic implant infection. How to cite: Gauna A, Mercado L, Guzmán F. Antimicrobial characterization ...

Abstract	Proliferation of bacteria, such as Staphylococcus aureus and Staphylococcus epidermidis, on orthopedic implants has been a challenge in orthopedic surgery, highlighting the problem of the increasing antibiotic resistance and the necessity to develop new antimicrobial agents. In this sense, antimicrobial peptides are promising candidates, which can be attached to titanium surfaces in order to make them safer. Mytilus mussels are characterized by adhering efficiently to a wide variety of surfaces, especially metallic ones, through adhesive proteins with a high content of dihydroxyphenylalanine, which is a post-translational modification of tyrosine. This work refers to the synthesis of a coating based on a bifunctional peptide that combines a sequence derived from the mussel foot protein-5 and the antimicrobial peptide pEM-2, derived from Bothrops asper snake venom. The adhesive properties of this bifunctional peptide were evaluated, as well as the adhesive sequence without the pEM-2, using a Quartz Crystal Microbalance. The results showed that the presence of the antimicrobial peptide improved the adhesion; however, a loss of the bactericidal activity was observed. Even so, the adhesive sequence by itself exhibited an important antifouling activity, preventing S. aureus and S. epidermidis adhesion to titanium by 75% and 45%, respectively, although the result against S. epidermidis was not significant. A 13-residue peptide derived from a natural and biocompatible source, like a Mytilus mussel adhesive protein, could be projected as a protective agent on titanium surfaces against S. aureus and S. epidermidis, being responsible for two thirds of the cases of orthopedic implant infection. How to cite: Gauna A, Mercado L, Guzmán F. Antimicrobial characterization of a titanium coating derived from mussel-glue and Bothrops asper snake venom for the prevention of implant-associated infections caused by Staphylococcus. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2022.02.001.
Keywords	Bothrops ; Mytilus ; Staphylococcus epidermidis ; adhesion ; antibacterial properties ; antibiotic resistance ; antifouling activities ; antimicrobial peptides ; biotechnology ; dihydroxyphenylalanine ; mussels ; orthopedics ; post-translational modification ; prostheses ; quartz crystal microbalance ; snake venoms ; surgery ; titanium ; tyrosine
Language	English
Dates of publication	2022-03
Size	p. 31-38.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2020598-3
ISSN	0717-3458
ISSN	0717-3458
DOI	10.1016/j.ejbt.2022.02.001
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Article ; Online: Assessment of learning in online academic programs from the digital transformation impelled by Covid-19

Ana Judith Paredes-Chacín / Luz Adriana Vargas-Escobar / Alicia Inciarte-González / Carolina Mercado-Porras

Revista de Ciencias Sociales, Vol 29, Iss

2023 Volume 1

Abstract: The objective of the study is to determine the stages of the evaluation of learning in online academic programs as a result of the digital transformation impelled by the Covid-19 pandemic in higher education. It is based on the digital transformation ... ...

Abstract	The objective of the study is to determine the stages of the evaluation of learning in online academic programs as a result of the digital transformation impelled by the Covid-19 pandemic in higher education. It is based on the digital transformation adopted in higher education since the Covid-19 pandemic, which accelerated the renewal of existing curricular programs and teaching-learning processes with a fragmented evaluation process. Through an explanatory research - purposeful and qualitative approach, the variables and metric indicators of pertinence, relevance and frequency of production of literature associated with the theme are studied to process the data. Likewise, a questionnaire was designed for the pilot study, selecting 25 teachers as sample units from two accredited private higher education institutions in Colombia located in Santiago de Cali and Barranquilla. The findings determined a 0.946 margin of reliability of the instrument for its application in the second phase and a matrix of indicators and the system of relations on the components and stages of the evaluation system were generated. The systematized practice of the stages is proposed as a conclusive basis for the definition of metrics that determine the incidence on the application of the evaluation of learning from academic programs mediated by digital technologies.
Keywords	learning evaluation ; online training ; academic programs ; higher education ; digital technologies ; Political science (General) ; JA1-92 ; Social sciences (General) ; H1-99
Subject code	027
Language	Spanish
Publishing date	2023-02-01T00:00:00Z
Publisher	Universidad del Zulia
Document type	Article ; Online
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Article ; Online: Cystic fibrosis-related mortality trends in Mexico between 1999 and 2020.

Bustamante, Adriana E / Fernández, Lucía T / Rivas, Lissette C / Mercado-Longoria, Roberto

Pediatric pulmonology

2022 Volume 58, Issue 2, Page(s) 632–634

MeSH term(s)	Humans ; Cystic Fibrosis/epidemiology ; Mexico/epidemiology ; Cystic Fibrosis Transmembrane Conductance Regulator
Chemical Substances	Cystic Fibrosis Transmembrane Conductance Regulator (126880-72-6)
Language	English
Publishing date	2022-11-18
Publishing country	United States
Document type	Letter
ZDB-ID	632784-9
ISSN	1099-0496 ; 8755-6863
ISSN (online)	1099-0496
ISSN	8755-6863
DOI	10.1002/ppul.26239
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Article ; Online: Framework for the assessment of the safety culture in the oil and gas industry.

Rocha, Raoni / Duarte, Francisco / Lima, Francisco P A / Mercado, Marina / Araújo, Adriana / Garotti, Luciano / Campos, Magno

International journal of occupational safety and ergonomics : JOSE

2023 Volume 30, Issue 1, Page(s) 224–237

Abstract: Approaches to safety culture assessment may have many limits if supported exclusively by quantitative methods. Based on this, a research team developed a quantitative-qualitative approach to assess the maturity of the safety culture on an oil platform. ... ...

Abstract	Approaches to safety culture assessment may have many limits if supported exclusively by quantitative methods. Based on this, a research team developed a quantitative-qualitative approach to assess the maturity of the safety culture on an oil platform. To that end, the team sought to develop and test a method consisting of an initial ethnographic phase followed by four other distinct phases: definition of homogeneous groups; production of customized questionnaires; quantitative evaluation; and qualitative assessment. The results show the emergence of trends, from pre-defined themes in safety culture to specific levels of maturity for each of the homogeneous groups. At the same time, it was perceived that the maturity level of the groups is defined from the daily work practices developed by each one of them. This experience allowed us to propose a framework for assessing the maturity levels of safety culture for the oil and gas industry.
MeSH term(s)	Humans ; Safety Management ; Oil and Gas Industry ; Industry
Language	English
Publishing date	2023-12-27
Publishing country	England
Document type	Journal Article
ZDB-ID	1335568-5
ISSN	2376-9130 ; 1080-3548
ISSN (online)	2376-9130
ISSN	1080-3548
DOI	10.1080/10803548.2023.2293389
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Article ; Online: Variable thermal plasticity of leaf functional traits in Andean tropical montane forests.

Cox, Andrew J F / González-Caro, Sebastián / Meir, Patrick / Hartley, Iain P / Restrepo, Zorayda / Villegas, Juan C / Sanchez, Adriana / Mercado, Lina M

Plant, cell & environment

2023 Volume 47, Issue 3, Page(s) 731–750

Abstract: Tropical montane forests (TMFs) are biodiversity hotspots and provide vital ecosystem services, but they are disproportionately vulnerable to climate warming. In the Andes, cold-affiliated species from high elevations are being displaced at the hot end ... ...

Abstract	Tropical montane forests (TMFs) are biodiversity hotspots and provide vital ecosystem services, but they are disproportionately vulnerable to climate warming. In the Andes, cold-affiliated species from high elevations are being displaced at the hot end of their thermal distributions by warm-affiliated species migrating upwards from lower elevations, leading to compositional shifts. Leaf functional traits are strong indicators of plant performance and at the community level have been shown to vary along elevation gradients, reflecting plant adaptations to different environmental niches. However, the plastic response of such traits to relatively rapid temperature change in Andean TMF species remains unknown. We used three common garden plantations within a thermosequence in the Colombian Andes to investigate the warming and cooling responses of key leaf functional traits in eight cold- and warm-affiliated species with variable thermal niches. Cold-affiliated species shifted their foliar nutrient concentrations when exposed to warming, while all other traits did not significantly change; contrastingly, warm-affiliated species were able to adjust structural, nutrient and water-use efficiency traits from acquisitive to conservative strategies in response to cooling. Our findings suggest that cold-affiliated species will struggle to acclimate functional traits to warming, conferring warm-affiliated species a competitive advantage under climate change.
MeSH term(s)	Ecosystem ; Trees/physiology ; Tropical Climate ; Forests ; Plant Leaves/physiology
Language	English
Publishing date	2023-12-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	391893-2
ISSN	1365-3040 ; 0140-7791
ISSN (online)	1365-3040
ISSN	0140-7791
DOI	10.1111/pce.14778
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Article ; Online: Associations of salivary aldosterone levels during pregnancy with maternal blood pressure and birth weight-for-gestational age in a Mexico City birth cohort.

Galván-Valencia, Oscar / Sanders, Alison P / Ariza, Ana Carolina / Burris, Heather H / Ortiz-Panozo, Eduardo / Svensson, Katherine / Mercado-García, Adriana / Téllez-Rojo, Martha Maria / Wright, Robert O / Tamayo-Ortiz, Marcela

Journal of perinatology : official journal of the California Perinatal Association

2024

Abstract: Objective: To determine associations of maternal salivary aldosterone with blood pressure (BP) in pregnancy and infant birth weight-for-gestational age (BWGA).: Methods: We measured maternal salivary aldosterone, BP and BWGA z-scores in 471 Mexico ... ...

Abstract	Objective: To determine associations of maternal salivary aldosterone with blood pressure (BP) in pregnancy and infant birth weight-for-gestational age (BWGA). Methods: We measured maternal salivary aldosterone, BP and BWGA z-scores in 471 Mexico City pregnancy cohort participants and performed multivariable linear regression of BP and BWGA on log-aldosterone levels. Results: Log-aldosterone was positively associated with diastolic BP (β = 0.12 95% CI: 0.04, 0.21). There were no main effects of log-aldosterone on BWGA. However, we detected an interaction between log-aldosterone and BP in association with BWGA; higher log-aldosterone was associated with lower BWGA in the lowest (β = -0.12, 95% CI: -0.26, 0.02) and highest (β = -0.12, 95% CI: -0.29, 0.06) BP tertiles. In contrast, in the middle BP tertile the association was positive (β = 0.09, 95% CI: -0.02, 0.20), p for interaction = 0.03. Conclusion: Higher maternal salivary aldosterone is positively associated with diastolic BP and may affect fetal growth differently depending on concurrent maternal blood pressure.
Language	English
Publishing date	2024-03-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	645021-0
ISSN	1476-5543 ; 0743-8346
ISSN (online)	1476-5543
ISSN	0743-8346
DOI	10.1038/s41372-024-01909-3
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