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Article ; Online: Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament.

Yamaguchi, Tomoko / Miyata, Tomoko / Makino, Fumiaki / Namba, Keiichi

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2646, Page(s) 43–53

Abstract: The bacterial flagellum is a large assembly of about 30 different proteins and is divided into three parts: the filament that acts as a screw propeller, the hook as a universal joint, and the basal body as a rotary motor. In the case of Salmonella, the ... ...

Abstract	The bacterial flagellum is a large assembly of about 30 different proteins and is divided into three parts: the filament that acts as a screw propeller, the hook as a universal joint, and the basal body as a rotary motor. In the case of Salmonella, the filament length is 10-15 μm, which is more than ten times longer than the size of the cell. The filament is composed of only one component protein, flagellin, and is made of 11 protofilaments. The filament can form 12 different supercoiled structures as polymorphic forms. Each protofilament can take either the L (left-handed) or R (right-handed) state, and the number ratio of the protofilaments in these two states determines the shape of the supercoil. Some point mutations in flagellin make the filament straight by making all the protofilaments in one of the two states. The straight filaments enable us to use their helical symmetries for structural analysis by electron cryomicroscopy (cryoEM) and single particle image analysis. Here, we describe the methods for the purification of the flagellar filament and cryoEM data collection and image analysis.
MeSH term(s)	Flagellin/chemistry ; Cryoelectron Microscopy ; Flagella/metabolism ; Salmonella/metabolism ; Image Processing, Computer-Assisted ; Bacterial Proteins/metabolism
Chemical Substances	Flagellin (12777-81-0) ; Bacterial Proteins
Language	English
Publishing date	2023-02-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3060-0_5
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Article ; Online: Nitroxide-Mediated Controlled Radical Copolymerization of α-Trifluoromethylstyrenes with Styrenes.

Kanbara, Tadashi / Ito, Yuriko / Yamaguchi, Airi / Yajima, Tomoko

Molecules (Basel, Switzerland)

2024 Volume 29, Issue 6

Abstract: Fluorinated polymers are important materials in everyday life; however, most monomers of widely used fluoropolymers are gaseous, and their polymerization is difficult in an ordinary laboratory. Therefore, partially fluorinated polymers have recently been ...

Abstract	Fluorinated polymers are important materials in everyday life; however, most monomers of widely used fluoropolymers are gaseous, and their polymerization is difficult in an ordinary laboratory. Therefore, partially fluorinated polymers have recently been reported. As an easy-to-handle fluorine-containing monomer, α-trifluoromethylstyrene (TFMST) can be used to produce partially fluorinated polymers with trifluoromethyl groups in the main chain; however, TFMST does not homopolymerize, and there are limited reports on its copolymerization with styrene (ST). In this study, we applied the controlled radical polymerization method, which is effective for the polymerization of ST, to the copolymerization of TFMST and ST. We also showed that nitroxide-mediated polymerization is effective. The content ratio of TFMST in the TFMST-ST copolymer can be controlled between 10% and 40% by changing its monomer ratio. Additionally, the polymerization of TFMST and ST with substituents was performed to increase structural variations. The thermal stability as well as water and oil repellency of the synthesized polymers with different composition ratios and substituents were also evaluated.
Language	English
Publishing date	2024-03-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules29061214
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Article: [Implementation of Clinical Practice in Adult Nursing (Chronic Care) During the COVID-19 Pandemic: Report on the Development and Implementation of a Training Program for Clinical Shadowing].

Hagiwara, Tomoko / Yamaguchi, Kyohei

Journal of UOEH

2022 Volume 44, Issue 3, Page(s) 313–319

Abstract: Before the outbreak of COVID-19, "Clinical Practice in Adult Nursing (Chronic Care)", a professional course to acquire basic nursing practice skills for the care of adult patients with chronic diseases, was conducted on site in the wards of internal ... ...

Abstract	Before the outbreak of COVID-19, "Clinical Practice in Adult Nursing (Chronic Care)", a professional course to acquire basic nursing practice skills for the care of adult patients with chronic diseases, was conducted on site in the wards of internal medicine, outpatient departments, and the admissions support office for a total of three weeks. Due to the spread of the COVID-19 epidemic, however, clinical practice was carried out online from May 2020, and was resumed on site at our university hospital in October 2020. A restriction was placed on the length of time spent at the hospital for on-site training, referring to the training standards based on our university's new coronavirus response manual, and a training program was developed for hospital ward training, consisting mainly of shadowing practice performed without speaking or coming into contact with patients. In principle, two students were assigned to one patient: one in the morning and the other in the afternoon. In addition, for practicing the acquisition of patient information through conversation and the provision of nursing assistance, the perspectives used in the process of shadowing were clearly defined so that students could maintain active attitudes in clinical practice, and support was provided so that students could have the experience of engaging with patients, using nurses as mediators. In the results, the students evaluated the contents of the prepared assistance based on their own nursing plan in reference to the practice of the nurse as a mediator, and compared that with the assistance practiced by the nurse based on clinical judgment, which contributed to acquiring practical learning supported by the grounds for clinical judgment. However, the students could not experience the process of building supportive relationships and the implementation of nursing skills, which remained an issue.
MeSH term(s)	Adult ; COVID-19 ; Humans ; Learning ; Pandemics ; Students
Language	Japanese
Publishing date	2022-09-12
Publishing country	Japan
Document type	Journal Article
ZDB-ID	632724-2
ISSN	2187-2864 ; 0387-821X
ISSN (online)	2187-2864
ISSN	0387-821X
DOI	10.7888/juoeh.44.313
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Article: Real-World Use of Albutrepenonacog Alfa, A Recombinant Coagulation Factor IX Albumin Fusion Protein, for Personalized Prophylaxis in Japanese Individuals With Hemophilia B: A Case Series.

Nagao, Azusa / Bingo, Masato / Yamaguchi, Tomoko / Fukutake, Katsuyuki

Cureus

2023 Volume 15, Issue 1, Page(s) e33573

Abstract: Currently, the mainstay of disease management for hemophilia B, a hemorrhagic disease caused by a congenital deficiency or molecular abnormalities of blood coagulation factor IX (FIX), is prophylaxis using FIX concentrate. On-demand injections of FIX ... ...

Abstract	Currently, the mainstay of disease management for hemophilia B, a hemorrhagic disease caused by a congenital deficiency or molecular abnormalities of blood coagulation factor IX (FIX), is prophylaxis using FIX concentrate. On-demand injections of FIX concentrate may also be required, even during prophylaxis, when a patient with hemophilia B is bleeding. Albutrepenonacog alfa (rFIX-FP) is a human albumin fusion gene recombinant FIX, which is administered once every seven, 14, or 21 days, depending on patient preferences and symptoms. Studies have demonstrated its efficacy and safety in a range of patients; however, to date, reports of real-world experiences of the use of rFIX-FP in Japan are limited. We present a case series of three Japanese individuals with moderately severe (FIX activity 1 to <2%) or severe (FIX activity <1%) hemophilia B who benefited from prophylaxis using rFIX-FP in our clinical practice setting. We highlighted the good effectiveness of rFIX-FP in a patient with moderately severe hemophilia B who required prophylaxis due to joint bleeding, which was causing deterioration of his left ankle joint, as well as in a patient with severe hemophilia B and atherothrombotic cerebral infarction, whose trough level had to be ≥5% for concomitant use of an antiplatelet drug, and in a patient with severe hemophilia B who was working in nursing care, which involved heavy labor and night shifts, and who had previously been treated with on-demand FIX concentrate. In all three cases, rFIX-FP improved disease symptoms, and the patients were able to maintain steady states of therapy due to the treatment characteristics of rFIX-FP, which stabilizes FIX at high trough levels.
Language	English
Publishing date	2023-01-09
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.33573
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Article ; Online: The maturation of iPS cell-derived brain microvascular endothelial cells by inducible-SOX18 expression.

Zhang, Hongyan / Yamaguchi, Tomoko / Kawabata, Kenji

Fluids and barriers of the CNS

2023 Volume 20, Issue 1, Page(s) 10

Abstract: Background: Brain microvascular endothelial cells (BMECs) play a major role in the blood-brain barrier (BBB), and are critical for establishing an in vitro BBB model. Currently, iPSC-derived BMECs (iBMECs) have been used to construct in vitro BBB models ...

Abstract	Background: Brain microvascular endothelial cells (BMECs) play a major role in the blood-brain barrier (BBB), and are critical for establishing an in vitro BBB model. Currently, iPSC-derived BMECs (iBMECs) have been used to construct in vitro BBB models with physiological barrier functions, such as high trans-endothelial electrical resistance (TEER) and expression of transporter proteins. However, the relatively low p-glycoprotein (P-gp) level and a decrease in the efflux ratio of its substrates in iBMECs suggest their immature nature. Therefore, more mature iBMECs by optimizing the differentiation induction protocol is beneficial for establishing a more reliable in vitro BBB model for studying central nervous system (CNS) drug transport. Methods: To identify human brain endothelial cell fate-inducing factors, HUVEC was transfected with Zic3A-, Zic3B-, and Sox18-expressing lentivirus vector. Since SOX18 was found to induce BMEC properties, we used a Dox-inducible Tet-on system to express SOX18 during iBMEC differentiation and explored the impact of SOX18 expression on iBMEC maturation. Results: Sox18-mediated iBMECs achieved a higher TEER value than normal iBMECs (> 3000 Ω cm Conclusions: Inducible SOX18 expression in iBMECs gained BBB phenotypes, including high TEER values and upregulation of tight junction-related genes, endothelial cell (EC) markers, BBB transporters, and higher cell viability after treatment with puromycin. Collectively, we provide a differentiation method for the maturation of human iPS cell-derived BMECs with SOX18 expression, describing its contribution to form an in vitro BBB model for CNS drug transport studies.
MeSH term(s)	Humans ; Endothelial Cells/metabolism ; Induced Pluripotent Stem Cells/physiology ; Cells, Cultured ; Brain/blood supply ; Blood-Brain Barrier/metabolism ; SOXF Transcription Factors/metabolism
Chemical Substances	SOX18 protein, human ; SOXF Transcription Factors
Language	English
Publishing date	2023-02-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2595406-4
ISSN	2045-8118 ; 2045-8118
ISSN (online)	2045-8118
ISSN	2045-8118
DOI	10.1186/s12987-023-00408-5
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Article ; Online: A 6-Month Follow-up Study on Adherence and Satisfaction with Lower Limb Prostheses and Orthoses Delivered to Community-Dwelling People in Japan.

Yamaguchi, Tomoko / Kubota, Masafumi / Naruse, Hiroaki / Matsumine, Akihiko / Yamamura, Osamu / Tsuchiya, Hiroyuki

Progress in rehabilitation medicine

2023 Volume 8, Page(s) 20230034

Language	English
Publishing date	2023-09-27
Publishing country	Japan
Document type	Journal Article
ISSN	2432-1354
ISSN (online)	2432-1354
DOI	10.2490/prm.20230034
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Article ; Online: Hydropic leiomyoma-like ovarian tumor: a case report.

Yamaguchi, Tomoko / Kyozuka, Hyo / Kochi, Yoshihiro / Ito, Fumihiro / Odajima, Hajime / Suzuki, Daisuke / Nomura, Yasuhisa

Fukushima journal of medical science

2024 Volume 70, Issue 2, Page(s) 93–98

Abstract: Uterine leiomyomas, benign tumors common in reproductive-aged women, can display rare variants such as hydropic leiomyoma (HL), which exhibit unique histological features like zonal edema and increased vascularity. However, due to its rarity, ... ...

Abstract	Uterine leiomyomas, benign tumors common in reproductive-aged women, can display rare variants such as hydropic leiomyoma (HL), which exhibit unique histological features like zonal edema and increased vascularity. However, due to its rarity, comprehensive clinical knowledge about HL is limited. We report a case of a 49-year-old Japanese woman who was premenopausal and nulliparous, presenting with a two-year history of abdominal distension. An MRI scan revealed a 20 cm mass in the posterior part of the uterus, exhibiting characteristics suggestive of an ovarian tumor. During laparotomy, a cystic tumor connected with a swollen fibroid was found, and pathology confirmed HL. This case emphasizes that hydropic leiomyomas can mimic malignant tumors on ultrasonography due to their atypical features, necessitating additional evaluations using alternative imaging techniques or histopathological examinations for accurate diagnosis and appropriate management. The patient recovered uneventfully, broadening our understanding of HL's clinical presentation.
MeSH term(s)	Humans ; Female ; Middle Aged ; Leiomyoma/pathology ; Leiomyoma/diagnostic imaging ; Ovarian Neoplasms/pathology ; Ovarian Neoplasms/diagnostic imaging ; Uterine Neoplasms/pathology ; Uterine Neoplasms/diagnostic imaging ; Magnetic Resonance Imaging ; Diagnosis, Differential
Language	English
Publishing date	2024-03-15
Publishing country	Japan
Document type	Case Reports ; Journal Article
ZDB-ID	958255-1
ISSN	2185-4610 ; 0016-2590
ISSN (online)	2185-4610
ISSN	0016-2590
DOI	10.5387/fms.2023-22
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Article ; Online: Utility of salivary cortisol profile as a predictive biomarker in nurses' turnover risk: a preliminary study.

Yamaguchi, Shinya / Fujita, Tomoko / Kato, Shintaro / Yoshimitsu, Yuichi / Ito, Yoichi M / Yano, Rika

Journal of physiological anthropology

2024 Volume 43, Issue 1, Page(s) 1

Abstract: Background: Predicting nurse turnover risk is crucial due to the global nursing shortage; however, existing predictors, such as fatigue and burnout, lack objectivity. Salivary cortisol is a non-invasive marker of stress and fatigue, but its utility in ... ...

Abstract	Background: Predicting nurse turnover risk is crucial due to the global nursing shortage; however, existing predictors, such as fatigue and burnout, lack objectivity. Salivary cortisol is a non-invasive marker of stress and fatigue, but its utility in predicting nurse turnover risk is unknown. We examined whether salivary cortisol profiles across three different day shifts in a month are predictors of the extent of nurses' reluctance to stay in their current jobs. Methods: This preliminary longitudinal study followed forty female nurses who engaged in shift work at a university hospital for 3 months. Data at enrollment were collected including demographics, working conditions, chronic fatigue (the Japanese version of the Occupational Fatigue/Exhaustion Recovery Scale), and burnout (Japanese Burnout scale). Salivary cortisol was measured before the three different day shifts (after awakening) during the first month, and the means of these measurements were used as the cortisol profile. The extent of reluctance to stay was assessed using the numerical rating scale at 3 months. Results: Among the forty female nurses (mean [SD] age, 28.3 [5.1]), all completed follow-up and were included in the analysis. The cortisol profile was associated with the extent of reluctance to stay (P = 0.017), and this association was significant despite adjustments for chronic fatigue and burnout (P = 0.005). A multiple regression model with chronic fatigue, burnout, and job tenure explained 41.5% of the variation in reluctance to stay. When the cortisol profile was added to this model, the association of the cortisol profile was significant (P = 0.006) with an R Conclusions: This preliminary study conducted in an actual clinical setting indicated the potential of the salivary cortisol profile across three different day shifts in a month to predict nurses' reluctance to stay in their current jobs. The combination of subjective indicators and the cortisol profile would be useful in predicting nurses' turnover risk.
MeSH term(s)	Humans ; Female ; Adult ; Hydrocortisone ; Longitudinal Studies ; Fatigue Syndrome, Chronic ; Burnout, Professional ; Nurses ; Surveys and Questionnaires
Chemical Substances	Hydrocortisone (WI4X0X7BPJ)
Language	English
Publishing date	2024-01-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2248072-9
ISSN	1880-6805 ; 1880-6791 ; 1345-3475
ISSN (online)	1880-6805
ISSN	1880-6791 ; 1345-3475
DOI	10.1186/s40101-023-00349-w
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Article ; Online: An infected urachal cyst presenting as repeated cystitis in a child.

Fukuhara, Masahiro / Uchida, Yasuyuki / Yamaguchi, Yoshiki / Sato, Tomoe / Izaki, Tomoko

Pediatrics international : official journal of the Japan Pediatric Society

2022 Volume 64, Issue 1, Page(s) e15086

MeSH term(s)	Child ; Cystitis/diagnosis ; Humans ; Urachal Cyst/complications ; Urachal Cyst/diagnosis
Language	English
Publishing date	2022-03-23
Publishing country	Australia
Document type	Journal Article
ZDB-ID	1470376-2
ISSN	1442-200X ; 1328-8067
ISSN (online)	1442-200X
ISSN	1328-8067
DOI	10.1111/ped.15086
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Article ; Online: Utility of salivary cortisol profile as a predictive biomarker in nurses’ turnover risk

Shinya Yamaguchi / Tomoko Fujita / Shintaro Kato / Yuichi Yoshimitsu / Yoichi M. Ito / Rika Yano

Journal of Physiological Anthropology, Vol 43, Iss 1, Pp 1-

a preliminary study

2024 Volume 10

Abstract: Abstract Background Predicting nurse turnover risk is crucial due to the global nursing shortage; however, existing predictors, such as fatigue and burnout, lack objectivity. Salivary cortisol is a non-invasive marker of stress and fatigue, but its ... ...

Abstract	Abstract Background Predicting nurse turnover risk is crucial due to the global nursing shortage; however, existing predictors, such as fatigue and burnout, lack objectivity. Salivary cortisol is a non-invasive marker of stress and fatigue, but its utility in predicting nurse turnover risk is unknown. We examined whether salivary cortisol profiles across three different day shifts in a month are predictors of the extent of nurses’ reluctance to stay in their current jobs. Methods This preliminary longitudinal study followed forty female nurses who engaged in shift work at a university hospital for 3 months. Data at enrollment were collected including demographics, working conditions, chronic fatigue (the Japanese version of the Occupational Fatigue/Exhaustion Recovery Scale), and burnout (Japanese Burnout scale). Salivary cortisol was measured before the three different day shifts (after awakening) during the first month, and the means of these measurements were used as the cortisol profile. The extent of reluctance to stay was assessed using the numerical rating scale at 3 months. Results Among the forty female nurses (mean [SD] age, 28.3 [5.1]), all completed follow-up and were included in the analysis. The cortisol profile was associated with the extent of reluctance to stay (P = 0.017), and this association was significant despite adjustments for chronic fatigue and burnout (P = 0.005). A multiple regression model with chronic fatigue, burnout, and job tenure explained 41.5% of the variation in reluctance to stay. When the cortisol profile was added to this model, the association of the cortisol profile was significant (P = 0.006) with an R 2 of 0.529 (ΔR 2 = 0.114). Conclusions This preliminary study conducted in an actual clinical setting indicated the potential of the salivary cortisol profile across three different day shifts in a month to predict nurses’ reluctance to stay in their current jobs. The combination of subjective indicators and the cortisol profile would be useful in predicting nurses' ...
Keywords	Personnel turnover ; Nurses ; Saliva ; Cortisol ; Fatigue ; Burnout ; Physical anthropology. Somatology ; GN49-298
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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