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  1. Article ; Online: Data-Independent Acquisition and Label-Free Quantification for Quantitative Proteomics Analysis of Human Cerebrospinal Fluid.

    Makepeace, Karl A T / Rookyard, Alexander W / Das, Lipi / Vardarajan, Badri N / Chakrabarty, Jayanta K / Jain, Anu / Kang, Min Suk / Werth, Emily G / Reyes-Dumeyer, Dolly / Zerlin-Esteves, Marielba / Honig, Lawrence S / Mayeux, Richard / Brown, Lewis M

    Current protocols

    2024  Volume 4, Issue 3, Page(s) e1014

    Abstract: This article presents a practical guide to mass spectrometry-based data-independent acquisition and label-free quantification for proteomics analysis applied to cerebrospinal fluid, offering a robust and scalable approach to probing the proteomic ... ...

    Abstract This article presents a practical guide to mass spectrometry-based data-independent acquisition and label-free quantification for proteomics analysis applied to cerebrospinal fluid, offering a robust and scalable approach to probing the proteomic composition of the central nervous system. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Cerebrospinal fluid sample collection and preparation for mass spectrometry analysis Basic Protocol 2: Mass spectrometry sample analysis with data-independent acquisition Support Protocol: Data-dependent mass spectrometry and spectral library construction Basic Protocol 3: Analysis of mass spectrometry data.
    MeSH term(s) Humans ; Proteomics/methods ; Proteome/analysis ; Mass Spectrometry/methods ; Cerebrospinal Fluid Proteins/chemistry
    Chemical Substances Proteome ; Cerebrospinal Fluid Proteins
    Language English
    Publishing date 2024-04-25
    Publishing country United States
    Document type Journal Article
    ISSN 2691-1299
    ISSN (online) 2691-1299
    DOI 10.1002/cpz1.1014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Wnt/Frizzled signaling in angiogenesis.

    Zerlin, Marielba / Julius, Martin A / Kitajewski, Jan

    Angiogenesis

    2008  Volume 11, Issue 1, Page(s) 63–69

    Abstract: The roles of growth factors such as angiopoietin (Ang) and vascular endothelial growth factor (VEGF) in angiogenesis have been known for some time, yet we have just an incipient appreciation for the contribution of Wnts to this process. Cellular ... ...

    Abstract The roles of growth factors such as angiopoietin (Ang) and vascular endothelial growth factor (VEGF) in angiogenesis have been known for some time, yet we have just an incipient appreciation for the contribution of Wnts to this process. Cellular proliferation and polarity, apoptosis, branching morphogenesis, inductive processes, and the maintenance of stem cells in an undifferentiated, proliferative state are all regulated by Wnt signaling. The development and maintenance of vascular structures are dependent on all these processes, and their orchestration has, to some extent, been revealed in studies of VEGF and Ang receptors. Recent evidence links the Wnt/Frizzled signaling pathway to proper vascular growth in mammals but our knowledge of Wnt function in the vasculature is rudimentary. Further insight into vascular development and the process of angiogenesis depends on evaluating the function of novel endothelial regulatory pathways such as Wnt/Frizzled signaling.
    MeSH term(s) Animals ; Frizzled Receptors/physiology ; Humans ; Neovascularization, Physiologic/physiology ; Signal Transduction/physiology ; Wnt Proteins/physiology
    Chemical Substances Frizzled Receptors ; Wnt Proteins
    Language English
    Publishing date 2008-02-06
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 1484717-6
    ISSN 0969-6970
    ISSN 0969-6970
    DOI 10.1007/s10456-008-9095-3
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    Zs.A 5094: Show issues Location:
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  3. Article ; Online: The role of Notch and gamma-secretase inhibition in an ovarian cancer model.

    Shah, Monjri M / Zerlin, Marielba / Li, Blake Y / Herzog, Thomas J / Kitajewski, Jan K / Wright, Jason D

    Anticancer research

    2013  Volume 33, Issue 3, Page(s) 801–808

    Abstract: Background: The Notch pathway is dysregulated in ovarian cancer. We sought to examine the role of Notch and gamma-secretase (GS) inhibition in ovarian cancer.: Materials and methods: Established ovarian cancer cell lines were used. Quantitative ... ...

    Abstract Background: The Notch pathway is dysregulated in ovarian cancer. We sought to examine the role of Notch and gamma-secretase (GS) inhibition in ovarian cancer.
    Materials and methods: Established ovarian cancer cell lines were used. Quantitative polymerase chain reaction (qPCR) was used to determine the relative expression of Notch receptor and ligands. Effects of GS inhibition on proliferation, colony formation, and downstream effectors were examined via methylthiazole tetrazolium (MTT) and Matrigel assays, and qPCR, respectively. In vivo experiments with a GS inhibitor and cisplatin were conducted on nude mice. Tumors were examined for differences in microvessel density, proliferation, and apoptosis.
    Results: Notch3 was the most up-regulated receptor. The ligands JAGGED1 and DELTA-LIKE4 were both up-regulated. GS inhibition did not affect cellular proliferation or anchorage-independent cell growth over placebo. The GS inhibitor Compound-E reduced microvessel density in vivo.
    Conclusion: GS inhibition does not directly affect cellular proliferation in ovarian carcinoma, but Notch pathway blockade may result in angiogenic alterations that may be therapeutically important.
    MeSH term(s) Amyloid Precursor Protein Secretases/antagonists & inhibitors ; Animals ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Female ; Humans ; Mice ; Models, Biological ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/pathology ; Receptors, Notch/antagonists & inhibitors ; Receptors, Notch/physiology
    Chemical Substances Receptors, Notch ; Amyloid Precursor Protein Secretases (EC 3.4.-)
    Language English
    Publishing date 2013-01-24
    Publishing country Greece
    Document type Journal Article
    ZDB-ID 604549-2
    ISSN 1791-7530 ; 0250-7005
    ISSN (online) 1791-7530
    ISSN 0250-7005
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    Zs.A 1642: Show issues Location:
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  4. Article: Glial progenitors of the neonatal subventricular zone differentiate asynchronously, leading to spatial dispersion of glial clones and to the persistence of immature glia in the adult mammalian CNS.

    Zerlin, Marielba / Milosevic, Ana / Goldman, James E

    Developmental biology

    2004  Volume 270, Issue 1, Page(s) 200–213

    Abstract: The subventricular zone (SVZ) of the developing mammalian forebrain gives rise to astrocytes and oligodendrocytes in the neocortex and white matter, and neurons in the olfactory bulb in perinatal life. We have examined the developmental fates and spatial ...

    Abstract The subventricular zone (SVZ) of the developing mammalian forebrain gives rise to astrocytes and oligodendrocytes in the neocortex and white matter, and neurons in the olfactory bulb in perinatal life. We have examined the developmental fates and spatial distributions of the descendants of single SVZ cells by infecting them in vivo at postnatal day 0-1 (P0-1) with a retroviral "library". In most cases, individual SVZ cells gave rise to either oligodendrocytes or astrocytes, but some generated both types of glia. Members of glial clones can disperse widely through the gray and white matter. Progenitors continued to divide after stopping migration, generating clusters of related cells. However, the progeny of a single SVZ cell does not differentiate synchronously: individual clones contained both mature and less mature glia after short or long intervals. For example, progenitors that settled in the white matter generated three types of clonal oligodendrocyte clusters: those composed of only myelinating oligodendrocytes, of both myelinating oligodendrocytes and non-myelinating oligodendrocytes, or of only non-myelinating cells of the oligodendrocyte lineage. Thus, some progenitors do not fully differentiate, but remain immature and may continue to cycle well into adult life.
    MeSH term(s) Animals ; Animals, Newborn ; Cell Differentiation/physiology ; Cell Lineage ; Cell Movement ; Neuroglia/cytology ; Neuroglia/physiology ; Prosencephalon/cytology ; Prosencephalon/embryology ; Prosencephalon/growth & development ; Rats ; Rats, Sprague-Dawley ; Retroviridae/genetics ; Retroviridae/metabolism ; Staining and Labeling/methods ; Stem Cells/cytology ; Stem Cells/physiology
    Language English
    Publishing date 2004-06-01
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1114-9
    ISSN 1095-564X ; 0012-1606
    ISSN (online) 1095-564X
    ISSN 0012-1606
    DOI 10.1016/j.ydbio.2004.02.024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 508: Show issues Location:
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  5. Article: Some glial progenitors in the neonatal subventricular zone migrate through the corpus callosum to the contralateral cerebral hemisphere.

    Kakita, Akiyoshi / Zerlin, Marielba / Takahashi, Hitoshi / Goldman, James E

    The Journal of comparative neurology

    2003  Volume 458, Issue 4, Page(s) 381–388

    Abstract: The great majority of glial cells of the mammalian forebrain are generated in the perinatal period from progenitors in the subventricular zone (SVZ). We investigated the migration of progenitors from the neonatal (postnatal day 0, P0) rat forebrain SVZ ... ...

    Abstract The great majority of glial cells of the mammalian forebrain are generated in the perinatal period from progenitors in the subventricular zone (SVZ). We investigated the migration of progenitors from the neonatal (postnatal day 0, P0) rat forebrain SVZ by labeling them in vivo with a green fluorescence protein (GFP) retrovirus and monitoring their movements by time-lapse video microscopy in P3 slices. We identified a small number of progenitors that migrated tangentially within the corpus callosum (CC) and crossed the midline. These cells retained a relatively uniform morphology: the leading process was extended toward the contralateral side but showed no process branching or turning away from the migratory direction. Net migration requires the elongation of the leading process and nuclear translocation, and the migrating cells in the CC showed both modes. We confirmed the presence of unmyelinated axon bundles within the P3 CC, but failed to detect any radially directed glial processes (vimentin- or GLAST-immunolabeled fibers) spanning through the CC. Confocal images showed a close proximity between neurofilament-immunolabeled axons and the leading process of the GFP-expressing progenitors in the CC. The destination of the callosal fibers was examined by applying DiI to the right cingulum; the labeled fibers ran throughout the CC and reached the left cingulate and motor areas. The distribution and final fates of the retrovirus-labeled cells were examined in P28 brains. A small proportion of the labeled cells were found in the contralateral hemisphere, where, as oligodendrocytes and astrocytes, they colonized predominantly the cortex and the underlying white matter of the cingulate and secondary motor areas. The distribution pattern appears to coincide well with the projection direction of the callosal fibers. Thus, glial progenitors migrate across the CC, presumably in conjunction with unmyelinated axons, to colonize the contralateral hemisphere.
    MeSH term(s) Animals ; Animals, Newborn ; Axons/physiology ; Cell Movement/physiology ; Corpus Callosum/cytology ; Corpus Callosum/growth & development ; Functional Laterality/physiology ; Green Fluorescent Proteins ; Immunohistochemistry ; Luminescent Proteins ; Microscopy, Confocal ; Neuroglia/cytology ; Neuroglia/metabolism ; Rats ; Rats, Sprague-Dawley ; Retroviridae ; Stem Cells/physiology ; Telencephalon/growth & development
    Chemical Substances Luminescent Proteins ; Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2003-04-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 3086-7
    ISSN 1096-9861 ; 0021-9967 ; 0092-7317
    ISSN (online) 1096-9861
    ISSN 0021-9967 ; 0092-7317
    DOI 10.1002/cne.10597
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  6. Article ; Online: Fate Determination and Migration of Progenitors in the Postnatal Mammalian CNS

    Goldman, James E. / Zerlin, Marielba / Newman, Sharon / Zhang, Lei / Gensert, JoAnn

    Developmental Neuroscience

    1997  Volume 19, Issue 1, Page(s) 42–48

    Abstract: Using replication-deficient retroviruses to transfer marker genes into immature cells, we have characterized spatial and temporal patterns of glial progenitor migration and differentiation in the early postnatal rat forebrain and cerebellum, and ... ...

    Abstract Using replication-deficient retroviruses to transfer marker genes into immature cells, we have characterized spatial and temporal patterns of glial progenitor migration and differentiation in the early postnatal rat forebrain and cerebellum, and interneuron differentiation in the cerebellum. Progenitors do not migrate randomly, but follow discrete paths, largely confined to a coronal plane in forebrain and a sagittal plane in cerebellum. Radial glia provide one substrate for migration. In vitro studies suggest that radial glia contribute a permissive pathway along which migratory progenitors can travel and that contact with radial glia keeps progenitors in an immature, migratory state. Local environmental cues that progenitors encounter during migration may influence fate decisions substantially. Not all progenitors differentiate; some remain in an immature, proliferative state in which they do not complete differentiation, but can be induced to do so by pathological conditions.
    Keywords Development ; Migration ; Astrocyte ; Oligodendrocyte ; Radial glia ; Interneuron
    Language English
    Publisher S. Karger AG
    Publishing place Basel
    Publishing country Switzerland
    Document type Article ; Online
    ZDB-ID 556887-0
    ISSN 1421-9859 ; 0378-5866 ; 0378-5866
    ISSN (online) 1421-9859
    ISSN 0378-5866
    DOI 10.1159/000111184
    Database Karger publisher's database

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